scholarly journals Three-dimensional fast elemental mapping by soft X-ray dual-energy focal stacks imaging

2021 ◽  
Vol 28 (3) ◽  
Author(s):  
Limei Ma ◽  
Zijian Xu ◽  
Zhi Guo ◽  
Benjamin Watts ◽  
Jinyou Lin ◽  
...  
Keyword(s):  
Spatial Distribution ◽  
Materials Science ◽  
Three Dimensional ◽  
Dual Energy ◽  
Acquisition Time ◽  
Imaging Method ◽  
Reconstruction Method ◽  
Elemental Mapping ◽  
Contrast Imaging ◽  
X Ray

The three-dimensional (3D) dual-energy focal stacks (FS) imaging method has been developed to quickly obtain the spatial distribution of an element of interest in a sample; it is a combination of the 3D FS imaging method and two-dimensional (2D) dual-energy contrast imaging based on scanning transmission soft X-ray microscopy (STXM). A simulation was firstly performed to verify the feasibility of the 3D elemental reconstruction method. Then, a sample of composite nanofibers, polystyrene doped with ferric acetylacetonate [Fe(acac)3], was further investigated to quickly reveal the spatial distribution of Fe(acac)3 in the sample. Furthermore, the data acquisition time was less than that for STXM nanotomography under similar resolution conditions and did not require any complicated sample preparation. The novel approach of 3D dual-energy FS imaging, which allows fast 3D elemental mapping, is expected to provide invaluable information for biomedicine and materials science.

scholarly journals Three-dimensional Construction of Micrometer Level in Rat Stomach by Synchrotron RadiationThree-dimensional Construction of Micrometer Level in Rat Stomach by Synchrotron Radiation

2020 ◽  
Author(s):  
Qiang Tao ◽  
Chen-Chen Gao ◽  
Xue-Hong Tong ◽  
Shizhen Yuan ◽  
Tian-tian Wang ◽  
...  
Keyword(s):  
Synchrotron Radiation ◽  
Phase Contrast ◽  
Three Dimensional ◽  
Image Resolution ◽  
Phase Contrast Imaging ◽  
Imaging Method ◽  
Rat Stomach ◽  
Contrast Imaging ◽  
X Ray ◽  
3 Dimensional

Abstract Objectives This article shows an imaging method of the stomach that does not use imaging agents. X-ray phase-contrast images of different stages of gastric development were taken using X-ray in-line phase-contrast imaging (XILPCI). The aim of the study was to demonstrate that XILPCI is a micron imaging method for gastric structures. Methods The stomachs of 4-, 6- and 12-week-old rats were removed and cleaned. XILPCI has 1000 times greater soft tissue contrast than that of X-ray traditional absorption radiography. The projection images of the rats’ stomachs were recorded by an XILPCI charge coupled device (CCD) at 9 μm image resolution. Results The X-ray in-line phase-contrast images of the different stages of rat gastric specimens clearly showed the gastric architectures and the details of the gastroduodenal region. 3-dimensional stomach anatomical structure images were reconstruction. Conclusion The reconstructed gastric 3D images can clearly display the internal structure of the stomach. XILPCI may be a useful method for medical research in the future. Keywords: Synchrotron radiation phase-contrast imaging, 3-dimensional gastric structure images

scholarly journals Three-dimensional Construction of Micrometer Level in Rat Stomach by Synchrotron Radiation

2020 ◽  
Author(s):  
Qiang Tao ◽  
Chen-Chen Gao ◽  
Xue-Hong Tong ◽  
Shi-Zhen Yuan ◽  
Jingdong Xu
Keyword(s):  
Synchrotron Radiation ◽  
Phase Contrast ◽  
Structural Changes ◽  
Three Dimensional ◽  
Image Resolution ◽  
Imaging Method ◽  
Contrast Imaging ◽  
X Ray ◽  
3 Dimensional ◽  
Tissue Contrast

Abstract Background: The structural changes of gastric mucosa are considered as an important window of early gastric lesions. This article shows an imaging method of the stomach that does not use imaging agents. X-ray phase-contrast images of different stages of gastric development were taken using micrometer level X-ray in-line phase-contrast imaging (XILPCI) technique on synchrotron radiation facility. The aim of the study was to demonstrate that the imaging technique is an appropriate method for micron imaging of the gastric structures.Methods: The stomachs of 4-, 6- and 12-week-old rats were removed and cleaned. XILPCI has 1000 times greater soft tissue contrast than that of X-ray traditional absorption radiography. The projection images of the rats stomachs were recorded by an XILPCI charge coupled device (CCD) at 9 μm image resolution.Results: The X-ray in-line phase-contrast images of the different stages of rats’ gastric specimens clearly showed the gastric architectures and the details of the gastro-duodenal region. 3-dimensional stomach anatomical structure images were reconstruction. Conclusion: The reconstructed gastric 3D images can clearly display the internal structure of the stomach. XILPCI may be a useful method for medical research in the future.

scholarly journals Three-dimensional Construction of Micrometer Level in Rat Stomach by Synchrotron Radiation

2020 ◽  
Author(s):  
Qiang Tao ◽  
Chen-Chen Gao ◽  
Xue-Hong Tong ◽  
Shi-Zhen Yuan ◽  
Jingdong Xu
Keyword(s):  
Synchrotron Radiation ◽  
Phase Contrast ◽  
Structural Changes ◽  
Three Dimensional ◽  
Image Resolution ◽  
Imaging Method ◽  
Contrast Imaging ◽  
X Ray ◽  
3 Dimensional ◽  
Tissue Contrast

Abstract Background: The structural changes of gastric mucosa are considered as an important window of early gastric lesions. This article shows an imaging method of the stomach that does not use imaging agents. X-ray phase-contrast images of different stages of gastric development were taken using micrometer level X-ray in-line phase-contrast imaging (XILPCI) technique on synchrotron radiation facility. The aim of the study was to demonstrate that the imaging technique is an appropriate method for micron imaging of the gastric structures.Methods: The stomachs of 4-, 6- and 12-week-old rats were removed and cleaned. XILPCI has 1000 times greater soft tissue contrast than that of X-ray traditional absorption radiography. The projection images of the rats stomachs were recorded by an XILPCI charge coupled device (CCD) at 9 μm image resolution.Results: The X-ray in-line phase-contrast images of the different stages of rats’ gastric specimens clearly showed the gastric architectures and the details of the gastro-duodenal region. 3-dimensional stomach anatomical structure images were reconstruction.Conclusion: The reconstructed gastric 3D images can clearly display the internal structure of the stomach. XILPCI may be a useful method for medical research in the future.

scholarly journals Three-Dimensional Construction of Micrometer Level in Rat Stomach by Synchrotron Radiation

2020 ◽  
Author(s):  
Qiang Tao ◽  
Chen-Chen Gao ◽  
Xue-Hong Tong ◽  
Shi-Zhen Yuan ◽  
Jingdong Xu
Keyword(s):  
Synchrotron Radiation ◽  
Phase Contrast ◽  
Structural Changes ◽  
Three Dimensional ◽  
Image Resolution ◽  
Imaging Method ◽  
Contrast Imaging ◽  
X Ray ◽  
3 Dimensional ◽  
Tissue Contrast

Abstract Background: The structural changes of gastric mucosa are considered as an important window of early gastric lesions. This article shows an imaging method of the stomach that does not use imaging agents. X-ray phase-contrast images of different stages of gastric development were taken using micrometer level X-ray in-line phase-contrast imaging (XILPCI) technique on synchrotron radiation facility. The aim of the study was to demonstrate that the imaging technique is an appropriate method for micron imaging of the gastric structures.Methods: The stomachs of 4-, 6- and 12-week-old rats were removed and cleaned. XILPCI has 1000 times greater soft tissue contrast than that of X-ray traditional absorption radiography. The projection images of the rats’ stomachs were recorded by an XILPCI charge coupled device (CCD) at 9 μm image resolution.Results: The X-ray in-line phase-contrast images of the different stages of rat gastric specimens clearly showed the gastric architectures and the details of the gastro-duodenal region. 3-dimensional stomach anatomical structure images were reconstruction. Conclusion: The reconstructed gastric 3D images can clearly display the internal structure of the stomach. XILPCI may be a useful method for medical research in the future.

scholarly journals Three-dimensional construction of micrometer level in rat stomach by synchrotron radiation

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Qiang Tao ◽  
Chenchen Gao ◽  
Xuehong Tong ◽  
Shizhen Yuan ◽  
Jingdong Xu
Keyword(s):  
Synchrotron Radiation ◽  
Phase Contrast ◽  
Structural Changes ◽  
Three Dimensional ◽  
Image Resolution ◽  
Imaging Method ◽  
Contrast Imaging ◽  
X Ray ◽  
3 Dimensional ◽  
Tissue Contrast

Abstract Background The structural changes of gastric mucosa are considered as an important window of early gastric lesions. This article shows an imaging method of the stomach that does not use imaging agents. X-ray phase-contrast images of different stages of gastric development were taken using micrometer level X-ray in-line phase-contrast imaging (XILPCI) technique on synchrotron radiation facility. The aim of the study was to demonstrate that the imaging technique is an appropriate method for micron imaging of the gastric structures. Methods The stomachs of 4-, 6- and 12-week-old rats were removed and cleaned. XILPCI has 1000 times greater soft tissue contrast than that of X-ray traditional absorption radiography. The projection images of the rats stomachs were recorded by an XILPCI charge coupled device (CCD) at 9-μm image resolution. Results The X-ray in-line phase-contrast images of the different stages of rats’ gastric specimens clearly showed the gastric architectures and the details of the gastro-duodenal region. 3-dimensional (3D) stomach anatomical structure images were reconstruction. Conclusion The reconstructed gastric 3D images can clearly display the internal structure of the stomach. XILPCI may be a useful method for medical research in the future.

Study of the Pump of a Mosquito

2011 ◽  
Author(s):  
Kenji Kikuchi ◽  
Osamu Mochizuki
Keyword(s):  
Oral Cavity ◽  
Phase Contrast ◽  
Three Dimensional ◽  
Blood Flow Rate ◽  
Pressure Head ◽  
Imaging Method ◽  
Contrast Imaging ◽  
Pump Frequency ◽  
Pump System ◽  
X Ray

Structures and functions of a mosquito’s pump system were investigated experimentally to clarify the mechanistic aspects of this optimized bio-pump. The three-dimensional strucure of the pump that was reconstructed by 1500 slices of the mosquito’s head was obtained using a histopathological method. The mosquito’s pump motion is predictable, and the pump volume can be estimated by the three-dimensional structural observations. The pump volume of the pumps, an oral cavity pump and pharynx pump, were estimated as being 7.92×10−4mm3, 63.3×10−4mm3 respectively. The frequency of the mosquito’s pumps was predicted to be 2.09–16.7 Hz, and was estimated using the pump volume and the volumetric blood flow rate. The motion of the mosquito’s pumps was visualized directly by an x-ray phase contrast imaging method using synchrotron radiation. It was found that the mosquito’s pumps (i.e., the oral cavity pump and the pharynx pump) worked alternately with a 4.23 Hz pump frequency. Moreover, the phase difference of each pump was almost 0.5, suggesting that the mosquito’s pumps worked in opposite phases. In addition, the mosquito’s pumps were found to work as a inline pump, which is suitable for increasing the pressure head of pump.

Macroprobe Mode for the Study of Segregation in Steels

1990 ◽  
Vol 48 (2) ◽  
pp. 260-261
Author(s):  
Auclair Gilles ◽  
Benoit Danièle
Keyword(s):  
Mechanical Properties ◽  
Spatial Distribution ◽  
High Performance ◽  
Volume Fraction ◽  
Sheet Steel ◽  
Acquisition Time ◽  
Chemical Information ◽  
X Ray ◽  
Accurate Quantitative Analysis ◽  
Optical Micrographs

During these last 10 years, high performance correction procedures have been developed for classical EPMA, and it is nowadays possible to obtain accurate quantitative analysis even for soft X-ray radiations. It is also possible to perform EPMA by adapting this accurate quantitative procedures to unusual applications such as the measurement of the segregation on wide areas in as-cast and sheet steel products.The main objection for analysis of segregation in steel by means of a line-scan mode is that it requires a very heavy sampling plan to make sure that the most significant points are analyzed. Moreover only local chemical information is obtained whereas mechanical properties are also dependant on the volume fraction and the spatial distribution of highly segregated zones. For these reasons we have chosen to systematically acquire X-ray calibrated mappings which give pictures similar to optical micrographs. Although mapping requires lengthy acquisition time there is a corresponding increase in the information given by image anlysis.

Recent Developments In Monochromatic Microprobe X-Ray Fluorescence (MMXRF)

1998 ◽  
Vol 4 (S2) ◽  
pp. 378-379
Author(s):  
Z. W. Chen ◽  
D. B. Wittry
Keyword(s):  
Materials Science ◽  
High Vacuum ◽  
Three Dimensional ◽  
High Sensitivity ◽  
X Rays ◽  
Fluorescence Excitation ◽  
X Ray ◽  
Quantitative Microanalysis ◽  
Recent Developments ◽  
Fifth Order

A monochromatic x-ray microprobe based on a laboratory source has recently been developed in our laboratory and used for fluorescence excitation. This technique provides high sensitivity (ppm to ppb), nondestructive, quantitative microanalysis with minimum sample preparation and does not require a high vacuum specimen chamber. It is expected that this technique (MMXRF) will have important applications in materials science, geological sciences and biological science.Three-dimensional focusing of x-rays can be obtained by using diffraction from doubly curved crystals. In our MMXRF setup, a small x-ray source was produced by the bombardment of a selected target with a focused electron beam and a toroidal mica diffractor with Johann pointfocusing geometry was used to focus characteristic x-rays from the source. In the previous work ∼ 108 photons/s were obtained in a Cu Kα probe of 75 μm × 43 μm in the specimen plane using the fifth order reflection of the (002) planes of mica.

scholarly journals A Method to Improve Electron Density Measurement of Cone-Beam CT Using Dual Energy Technique

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Kuo Men ◽  
Jian-Rong Dai ◽  
Ming-Hui Li ◽  
Xin-Yuan Chen ◽  
Ke Zhang ◽  
...  
Keyword(s):  
Electron Density ◽  
Cone Beam Ct ◽  
Density Measurement ◽  
Dual Energy ◽  
Cone Beam ◽  
Imaging Method ◽  
Basis Material ◽  
X Ray ◽  
Dual Energy Imaging ◽  
Set Up

Purpose. To develop a dual energy imaging method to improve the accuracy of electron density measurement with a cone-beam CT (CBCT) device.Materials and Methods. The imaging system is the XVI CBCT system on Elekta Synergy linac. Projection data were acquired with the high and low energy X-ray, respectively, to set up a basis material decomposition model. Virtual phantom simulation and phantoms experiments were carried out for quantitative evaluation of the method. Phantoms were also scanned twice with the high and low energy X-ray, respectively. The data were decomposed into projections of the two basis material coefficients according to the model set up earlier. The two sets of decomposed projections were used to reconstruct CBCT images of the basis material coefficients. Then, the images of electron densities were calculated with these CBCT images.Results. The difference between the calculated and theoretical values was within 2% and the correlation coefficient of them was about 1.0. The dual energy imaging method obtained more accurate electron density values and reduced the beam hardening artifacts obviously.Conclusion. A novel dual energy CBCT imaging method to calculate the electron densities was developed. It can acquire more accurate values and provide a platform potentially for dose calculation.

Full-field X-ray diffraction microscopy using polymeric compound refractive lenses

2014 ◽  
Vol 47 (6) ◽  
pp. 1882-1888 ◽  
Author(s):  
J. Hilhorst ◽  
F. Marschall ◽  
T. N. Tran Thi ◽  
A. Last ◽  
T. U. Schülli
Keyword(s):  
Imaging Techniques ◽  
Light And Electron Microscopy ◽  
Added Value ◽  
Acquisition Time ◽  
Imaging Method ◽  
X Ray Diffraction ◽  
Polymeric Compound ◽  
Full Field ◽  
X Ray ◽  
Diffraction Imaging

Diffraction imaging is the science of imaging samples under diffraction conditions. Diffraction imaging techniques are well established in visible light and electron microscopy, and have also been widely employed in X-ray science in the form of X-ray topography. Over the past two decades, interest in X-ray diffraction imaging has taken flight and resulted in a wide variety of methods. This article discusses a new full-field imaging method, which uses polymer compound refractive lenses as a microscope objective to capture a diffracted X-ray beam coming from a large illuminated area on a sample. This produces an image of the diffracting parts of the sample on a camera. It is shown that this technique has added value in the field, owing to its high imaging speed, while being competitive in resolution and level of detail of obtained information. Using a model sample, it is shown that lattice tilts and strain in single crystals can be resolved simultaneously down to 10−3° and Δa/a= 10−5, respectively, with submicrometre resolution over an area of 100 × 100 µm and a total image acquisition time of less than 60 s.

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