A Comparison of Manual and Robot Assisted Retinal Vein Cannulation in Chicken Chorioallantoic Membrane

Experimental data in this study demonstrate that integrin alpha v beta 3 is fundamentally involved in the maturation of blood vessels during embryonic neovascularization (vasculogenesis). Integrin alpha v beta 3 was specifically expressed on the surface of angioblasts during vessel development in quail embryos and vitronectin, a ligand for alpha v beta 3, localized to the basal surface of these cells. More importantly, microinjection of the anti-alpha v beta 3 monoclonal antibody, LM609, disrupted the normal pattern of vascular development. After exposure to LM609 the angioblasts in experimental embryos appeared as clusters of rounded cells lacking normal cellular protrusions. This led to disruption of lumen formation and abnormal vessel patterning. These findings demonstrate that during vasculogenesis ligation of integrin alpha v beta 3 on the surface of primordial endothelial cells is critical for the differentiation and maturation of blood vessels. Similar studies on chicken chorioallantoic membrane showed that LM609 blocks angiogenesis. Together the two studies suggest that integrin alpha v beta 3 plays a role in neovascularization of tissues.

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Chicken Chorioallantoic Membrane Angiogenesis Model

Methods in Molecular Biology - Cardiovascular Development ◽

10.1007/978-1-61779-523-7_5 ◽

2011 ◽

pp. 47-57 ◽

Cited By ~ 42

Author(s):

Domenico Ribatti

Keyword(s):

Chorioallantoic Membrane ◽

Chicken Chorioallantoic Membrane ◽

Angiogenesis Model

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P2.11-39 Multimodal Monitoring of Patient-Derived Early-Stage Lung Adenocarcinoma with the Chicken Chorioallantoic Membrane System

Journal of Thoracic Oncology ◽

10.1016/j.jtho.2019.08.1739 ◽

2019 ◽

Vol 14 (10) ◽

pp. S809

Author(s):

E. Koziolek ◽

B. Villegas ◽

G. Abdelhady ◽

J. Liu ◽

J. Barrio ◽

...

Keyword(s):

Lung Adenocarcinoma ◽

Early Stage ◽

Chorioallantoic Membrane ◽

Membrane System ◽

Multimodal Monitoring ◽

Chicken Chorioallantoic Membrane

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STAT3/5 Inhibitors Suppress Proliferation in Bladder Cancer and Enhance Oncolytic Adenovirus Therapy

International Journal of Molecular Sciences ◽

10.3390/ijms21031106 ◽

2020 ◽

Vol 21 (3) ◽

pp. 1106 ◽

Cited By ~ 7

Author(s):

Sruthi V. Hindupur ◽

Sebastian C. Schmid ◽

Jana Annika Koch ◽

Ahmed Youssef ◽

Eva-Maria Baur ◽

...

Keyword(s):

Bladder Cancer ◽

Cell Cycle Progression ◽

Chorioallantoic Membrane ◽

Oncolytic Adenovirus ◽

Cycle Progression ◽

Cellular Processes ◽

Division Cell ◽

Chicken Chorioallantoic Membrane ◽

Cam Model ◽

Phosphorylated Stat3

The JAK-STAT signalling pathway regulates cellular processes like cell division, cell death and immune regulation. Dysregulation has been identified in solid tumours and STAT3 activation is a marker for poor outcome. The aim of this study was to explore potential therapeutic strategies by targeting this pathway in bladder cancer (BC). High STAT3 expression was detected in 51.3% from 149 patient specimens with invasive bladder cancer by immunohistochemistry. Protein expression of JAK, STAT and downstream targets were confirmed in 10 cell lines. Effects of the JAK inhibitors Ruxolitinib and BSK-805, and STAT3/5 inhibitors Stattic, Nifuroxazide and SH-4-54 were analysed by cell viability assays, immunoblotting, apoptosis and cell cycle progression. Treatment with STAT3/5 but not JAK1/2 inhibitors reduced survival, levels of phosphorylated STAT3 and Cyclin-D1 and increased apoptosis. Tumour xenografts, using the chicken chorioallantoic membrane (CAM) model responded to Stattic monotherapy. Combination of Stattic with Cisplatin, Docetaxel, Gemcitabine, Paclitaxel and CDK4/6 inhibitors showed additive effects. The combination of Stattic with the oncolytic adenovirus XVir-N-31 increased viral replication and cell lysis. Our results provide evidence that inhibitors against STAT3/5 are promising as novel mono- and combination therapy in bladder cancer.

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