scholarly journals Magnetic Fe 3 O 4 @graphene oxide improves the therapeutic effects of embryonic stem cells on acute liver damage

2021 ◽  
Author(s):  
Tahereh Foroutan ◽  
Mohammad Zaman Kassaee ◽  
Mahdi Salari ◽  
Fatemeh Ahmady ◽  
Fatemeh Molavi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Graphene Oxide ◽  
Embryonic Stem Cells ◽  
Liver Damage ◽  
Embryonic Stem ◽  
Therapeutic Effects ◽  
Acute Liver Damage

Graphene Oxide Nanocolloids Induce Autophagy-Lysosome Dysfunction in Mouse Embryonic Stem Cells

2019 ◽  
Vol 15 (2) ◽  
pp. 340-351 ◽  
Author(s):  
Min Wei ◽  
Zhenfa Fu ◽  
Che Wang ◽  
Wei Zheng ◽  
Song Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Graphene Oxide ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells

Evaluating the cytotoxicity of graphene oxide using embryonic stem cells‐derived cells

2020 ◽  
Vol 108 (6) ◽  
pp. 1321-1328
Author(s):  
Le Hu ◽  
Yan Fu ◽  
Liyuan Rong ◽  
Xinji Yang ◽  
Yueyue Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Graphene Oxide ◽  
Embryonic Stem Cells ◽  
Embryonic Stem

Graphene oxide promotes the differentiation of mouse embryonic stem cells to dopamine neurons

Nanomedicine ◽  
2014 ◽  
Vol 9 (16) ◽  
pp. 2445-2455 ◽  
Author(s):  
Dehua Yang ◽  
Ting Li ◽  
Minghan Xu ◽  
Feng Gao ◽  
Juan Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Graphene Oxide ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Dopamine Neurons

Hepatocyte-Like Cells Can Be Derived from Human Umbilical Cord Blood and Embryonic Stem Cells: Tested in a Novel Mouse Model

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 3490-3490
Author(s):  
Ping Zhou ◽  
Ryan Lahey ◽  
Daniel Cortes ◽  
Yetunde Olusanya ◽  
Sarah Hohm ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cells ◽  
Mouse Model ◽  
Cord Blood ◽  
Liver Damage ◽  
Embryonic Stem ◽  
Human Cells ◽  
Embryoid Bodies ◽  
Recipient Mouse

Abstract Liver transplantation remains the only therapeutic option for many acute and chronic end-stage liver diseases. However, this approach is limited by a serious shortage of donor organs required for transplantation. Hepatocytes have been reported to be generated from cells not originated from liver, such as hematopoietic stem cells, mesenchymal stem cells and most recently embryonic stem cells. However, the frequency of these stem cell-derived hepatocytes is very low in most studies. Therefore, the significance of stem cell contribution to the repair of liver damage is still controversial. To further explore this potential, we used the beta-glucuronidase (GUSB)-null NOD/SCID/MPSVII mouse model for better identification of engrafted human cells. Enriched cord blood primitive cells (lineage depleted cells with high aldehyde dehydrogenase activity, ALDHhiLin−) were transplanted into irradiated NOD/SCID/MPSVII mice. One month after transplantation, carbon tetrachloride (CCl4) was administrated into the mice twice a week for 4 weeks to induce liver damage. In this model, ALDHhiLin− cells efficiently engrafted in the recipient mouse livers as demonstrated by GUSB positive immunohistological staining and the presence of human Alu DNA using PCR. The percentage of human cells in these livers ranged between 3% and 14.2% using quantitative real-time PCR. These engrafted cells improved recovery of the mice from toxic insult, and significantly increased the numbers of surviving mice. Furthermore, human liver-specific a-1-antitrypsin mRNA and albumin protein were expressed in the recipient livers. Interestingly, human vs. murine centromeric fluorescent in situ hybridization analysis on the liver sections demonstrated that most human cells were not fused to mouse cells. However, mouse nuclei were detected in the majority of the albumin-expressing cells, suggesting that fusion had occurred and was responsible for the appearance of donor derived hepatocyte-like cells. With the goal of achieving higher levels of liver reconstitution than had been possible using the adult stem cells, we began studying engraftment of human embryonic stem cells (hESC), which theoretically have the potential to regenerate any tissue. The H1 cell line was cultured on mouse embryonic fibroblasts then allowed to form embryoid bodies (EBs) in suspension culture for 7 days with or without further expansion and differentiation in attached culture for another month. EBs were dissociated into a single cell suspension and transplanted into NOD/SCID/MPSVII mice or NOD/SCID/IL2Rγ−/− mice via the tail vein after 300 RADs sublethal radiation with or without CCl4 administration. Two months post-transplantation, the human EB-derived cells were found to be well engrafted in the NOD/SCID/MPSVII mouse livers, spleens and kidneys, using the clear-cut enzymatic identification method for cells expressing normal levels of beta-glucuronidase in the mice, which are null for the enzyme. Human DNA was also detected in the recipient mouse liver. Most interestingly, human albumin-expressing cells were also found in the livers of engrafted mice. Our data indicate that the progeny of cord blood stem cells can significantly enhance survival of mice with severe liver damage, and that fusion can occur between transplanted and recipient cells. This could be a normal mechanism of liver repair, since hepatocytes exist normally as multinucleate cells. We also demonstrate that the progeny of hESC can be effectively dissociated and transplanted intravenously, then home to the liver and differentiate to the hepatocyte lineage in an immune deficient mouse model of liver damage.

scholarly journals Performance of the Polydopamine-Graphene Oxide Composite Substrate in the Osteogenic Differentiation of Mouse Embryonic Stem Cells

2021 ◽  
Vol 22 (14) ◽  
pp. 7323
Author(s):  
Na Young Shim ◽  
Jung Sun Heo
Keyword(s):  
Stem Cells ◽  
Graphene Oxide ◽  
Embryonic Stem Cells ◽  
Osteogenic Differentiation ◽  
Signaling Pathways ◽  
Embryonic Stem ◽  
Matrix Mineralization ◽  
Smad Signaling ◽  
Integrin Α5 ◽  
In Cells

Graphene oxide (GO) is a biocompatible material considered a favorable stem cell culture substrate. In this study, GO was modified with polydopamine (PDA) to facilitate depositing GO onto a tissue culture polystyrene (PT) surface, and the osteogenic performance of the PDA/GO composite in pluripotent embryonic stem cells (ESCs) was investigated. The surface chemistry of the PDA/GO-coated PT surface was analyzed by scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). A high cell viability of ESCs cultured on the PDA/GO composite-coated surface was initially ensured. Then, the osteogenic differentiation of the ESCs in response to the PDA/GO substrate was assessed by alkaline phosphatase (ALP) activity, intracellular calcium levels, matrix mineralization assay, and evaluation of the mRNA and protein levels of osteogenic factors. The culture of ESCs on the PDA/GO substrate presented higher osteogenic potency than that on the uncoated control surface. ESCs cultured on the PDA/GO substrate expressed significantly higher levels of integrin α5 and β1, as well as bone morphogenetic protein receptor (BMPR) types I and II, compared with the control groups. The phosphorylation of extracellular signal-regulated kinase (ERK)1/2, p38, and c-Jun-N-terminal kinase (JNK) mitogen-activated protein kinases (MAPKs) was observed in ESCs culture on the PDA/GO substrate. Moreover, BMP signal transduction by SMAD1/5/8 phosphorylation was increased more in cells on PDA/GO than in the control. The nuclear translocation of SMAD1/5/8 in cells was also processed in response to the PDA/GO substrate. Blocking activation of the integrin α5/β1, MAPK, or SMAD signaling pathways downregulated the PDA/GO-induced osteogenic differentiation of ESCs. These results suggest that the PDA/GO composite stimulates the osteogenic differentiation of ESCs via the integrin α5/β1, MAPK, and BMPR/SMAD signaling pathways.

scholarly journals Cytotoxicity Evaluation of Ammonia-Modified Graphene Oxide Particles in Lung Cancer Cells and Embryonic Stem Cells

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Milena Keremidarska-Markova ◽  
Kamelia Hristova-Panusheva ◽  
Tonya Andreeva ◽  
Giorgio Speranza ◽  
Dayong Wang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Stem Cells ◽  
Graphene Oxide ◽  
Embryonic Stem Cells ◽  
Cancer Cells ◽  
Human Lung ◽  
Embryonic Stem ◽  
A549 Cells ◽  
Lung Cancer Cells ◽  
Human Lung Cancer

Potential toxicity of graphene oxide (GO) is a subject of increasing research interest in the recent years. Here, we have evaluated the cytotoxicity of ammonia-modified GO (GO-NH2) and pristine GO particles in human lung cancer cells, A549 and embryonic stem cells, Lep3 exposed to different particles concentrations (0.1, 1, 10, 20, and 50 μg/ml) for different times (24 and 48h). Compared with GO, GO-NH2 particles possessed smaller size, positive surface charge and higher thickness. An increased propensity to aggregation in cell cultures was also found for GO-NH2 particles. Cytotoxicity evaluation revealed that GO-NH2 particles are more toxic than pristine GO. Applied at concentrations of 10, 20 and 50 μg/ml for 24h they affect significantly cell morphology of viable embryonic stem cells whereas human lung cancer A549 cells seem to be relatively more resistant to short-time exposure. After 48h exposure however cell proliferation of A549 cells was strongly suppressed in a dose-dependent manner while the proliferation ability of embryonic stem cells was not affected. These results suggested that both GO particles exert different degree of cytotoxicity which is time, dose and cell dependent. In general, ammonia-modified GO particles are more toxic than the pristine GO which should be taken into account for future biomedical applications.

Suspended graphene oxide nanosheets maintain the self-renewal of mouse embryonic stem cells via down-regulating the expression of Vinculin

Biomaterials ◽  
2018 ◽  
Vol 171 ◽  
pp. 1-11 ◽  
Author(s):  
Guoxin Jing ◽  
Zhaojie Wang ◽  
Xizhen Zhuang ◽  
Xiaolie He ◽  
Huijun Wu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Graphene Oxide ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
The Self ◽  
Graphene Oxide Nanosheets ◽  
Self Renewal ◽  
Suspended Graphene

Comparison of cell cycle components, apoptosis and cytoskeleton-related molecules and therapeutic effects of flavopiridol and geldanamycin on the mouse fibroblast, lung cancer and embryonic stem cells

Tumor Biology ◽  
2016 ◽  
Vol 37 (9) ◽  
pp. 12423-12440 ◽  
Author(s):  
Huseyin Aktug ◽  
Eda Acikgoz ◽  
Aysegul Uysal ◽  
Fatih Oltulu ◽  
Gulperi Oktem ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Cycle ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Fibroblast ◽  
Therapeutic Effects

Controlling the in vitro differentiation of embryonic stem cells for myocardial tissue engineering applications

2009 ◽  
Author(s):  
Dong-Bo Ou ◽  
Rui Chen ◽  
Xiong-Tao Liu ◽  
Jing-Jing Guo ◽  
Hong-Tao Wang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Myocardial Tissue ◽  
In Vitro Differentiation ◽  
Engineering Applications ◽  
Myocardial Tissue Engineering
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