Effect of microbial transglutaminase and setting condition on gel properties of blend fish protein isolate recovered by alkaline solubilisation/isoelectric precipitation

2018 ◽  
Vol 54 (3) ◽  
pp. 762-770
Author(s):  
Mehdi Abdollahi ◽  
Masoud Rezaei ◽  
Ali Jafarpour ◽  
Ingrid Undeland
2020 ◽  
Vol 67 (3) ◽  
Author(s):  
Abhilash Sasidharan ◽  
V. J. Michu ◽  
Chiranjiv Pradhan ◽  
S. Sabu

Threadfin bream (Nemipterus japonicus) is an important marine fish species used in the preparation of surimi. The waste generated from surimi production contains large quantity of recoverable proteins, which could be utilised for byproduct development. In this study, Isoelectric precipitation (IEP) technique as a method to recover proteins from fish processing waste was attempted. The alkali aided IEP process extracted the fish protein isolate (FPI) efficiently with a yield of 84.13±0.11%. The highest protein yield was recorded at a homogenised sample to water ratio of 1:9. Maximum protein content was found at pH 13 with 19.63 ±0.52% yield. FPI extracted had higher protein content, less ash and low lipid contents compared to the fish processing waste. The mean yield and protein composition of FPI extracted atdifferent treatments of pH and sample to water ratiowere found to be significantly different (p<0.05). Sample to water ratio of 1:9 and pH 13 was found to be the best combination among the different treatments attempted for FPI extraction from threadfin bream processing waste.


10.4194/afs16 ◽  
2021 ◽  
Vol 01 (01) ◽  
pp. AFS16-AFS16
Author(s):  
Gülsün Özyurt ◽  
Ahmet Faruk Yesilsu

Fish protein isolates extracted from underutilised fish species were used for coating material of anchovy oils and their nutritive value was investigated in this study. For this purpose, Klunzinger’s ponyfish (Equulites klunzingeri) proteins were extracted by using pH shifting process. Micro particles were prepared with anchovy oil (Engraulis encrasicolus) as core material (10%), and as wall materials a ratio of 5% and 10% fish protein isolate (FPI) was used. Maltodextrin (DE: 18:20) was added to both groups in a ratio of 10%. The emulsions were fed immediately into a Buchi Mini Spray Dryer (B290, Switzerland). The inlet temperatures, feed rate and aspiration rate were maintained at 160 oC, 15 mL/min and 35m3/h, respectively. The lipid, protein and moisture contents of anchovy oil microcapsules containing 5% FPI and 10% FPI were found as 43.76-43.09%, 4.34- 9.82% and 3.95-3.92%, respectively. The main amino acids in microcapsule samples were lysine, glutamic acid, and leucine which constituted in the range of 349-578 mg/100 g sample for microcapsules containing 5% FPI, and 805-1547 mg/100 g sample for microcapsules containing 10% FPI. In addition to that, essential and non-essential amino acids (E/NE) ratio for microencapsulated fish oil with 5% FPI and 10% FPI were determined 0.92 and 0.95, respectively. As a result of this study, it can be concluded that the addition of fish protein isolate enhanced the nutritive value of microencapsulated fish oil


2014 ◽  
Vol 39 ◽  
pp. 58-67 ◽  
Author(s):  
Yasir Ali Arfat ◽  
Soottawat Benjakul ◽  
Thummanoon Prodpran ◽  
Kazufumi Osako

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