A CONTINUOUS CULTURE STUDY OF PHOSPHATE UPTAKE, GROWTH RATE AND POLYPHOSPHATE IN SCENEDESMUS SP.1

1973 ◽  
Vol 9 (4) ◽  
pp. 495-506 ◽  
Author(s):  
G-Yull Rhee
Keyword(s):  
Growth Rate ◽  
Continuous Culture ◽  
Phosphate Uptake ◽  
Culture Study

CONTINUOUS CULTURE OF BRUCELLA ABORTUS S.19

1961 ◽  
Vol 7 (4) ◽  
pp. 491-505 ◽  
Author(s):  
Andreas H. W. Hauschild ◽  
Hilliard Pivnick
Keyword(s):  
Growth Rate ◽  
Continuous Culture ◽  
Dilution Rate ◽  
Brucella Abortus ◽  
Specific Growth ◽  
Limiting Factor ◽  
Continuous Growth ◽  
Viable Cells ◽  
Metabolic Products ◽  
Growth Of Bacteria

An apparatus is described for the continuous growth of bacteria. Brucella abortus S.19 has been grown in continuous culture for periods up to 3 weeks with populations up to 2 × 1011viable cells per ml and without the establishment of nonsmooth variants.Concentrations between 3 × 109and 2 × 1011cells per ml could be maintained as a function of the dilution rate without the requirement of a known limiting factor in the medium. In a series of steady-state conditions, the specific growth rate increased steadily up to 0.28 hour−1with decreasing population levels.Incidence of mutants was governed by the dilution rate and could also be reduced by various chelating substances.In continuous growth combined with continuous dialysis, population levels were approximately twice those obtained in continuous growth without dialysis. The effect of dialysis appears to be the continuous removal of growth-limiting metabolic products.

Evolution of Escherichia coli During Growth in a Constant Environment

Genetics ◽  
1987 ◽  
Vol 116 (3) ◽  
pp. 349-358
Author(s):  
Robert B Helling ◽  
Christopher N Vargas ◽  
Julian Adams
Keyword(s):  
Escherichia Coli ◽  
Growth Rate ◽  
Specific Growth Rate ◽  
Continuous Culture ◽  
Specific Growth ◽  
Maximum Specific Growth Rate ◽  
Constant Environment ◽  
Single Clone ◽  
Mixed Populations

ABSTRACT Populations of Escherichia coli, initiated with a single clone and maintained for long periods in glucose-limited continuous culture, developed extensive polymorphisms. In one population, examined after 765 generations, two majority and two minority types were identified. Stable mixed populations were reestablished from the isolated strains. Factors involved in the development of this polymorphism included differences in the maximum specific growth rate and in the transport of glucose, and excretion of metabolites by some clones which were utilized by minority clones.

Investigation of a nutrient-growth model using a continuous culture of natural phytoplankton

1978 ◽  
Vol 58 (4) ◽  
pp. 923-941 ◽  
Author(s):  
K. J. Jones ◽  
P. Tett ◽  
A. C. Wallis ◽  
B. J. B. Wood
Keyword(s):  
Growth Rate ◽  
Continuous Culture ◽  
Algal Growth ◽  
Nutrient Status ◽  
Inorganic Phosphorus ◽  
Particulate Phosphorus ◽  
Particulate Carbon ◽  
Particulate Nitrogen ◽  
Rate Of Increase ◽  
Dominance Pattern

Phytoplankton from Loch Creran, Argyll was maintained in continuous culture for 41 days. During most of this time the mixture of species retained the diversity and dominance pattern typical of summer phytoplankton in the loch, notwithstanding the manipulation of algal nutrient status to bring about phosphorus control of growth. Results suggest that most detritus was washed out early in the experiment.The aim of the experiment was to test the applicability to natural multispecies phytoplankton of part of Droop's (1974, 1975) model of nutrient-limited algal growth. ‘Quasi-steady states’ with respect to concentration of chlorophyll, particulate carbon (PC), particulate phosphorus (PP) and particulate nitrogen (PN), wereobserved at two levels of input of dissolved inorganic phosphorus. At other times the algal phosphorus quota (estimated from the ratio of PP to PC) varied from about 0·002 to 0·015 atoms P per atom C. Specific growth rate was estimated from the rate of increase of PC. During part of the experiment, growth rate was linearly related to reciprocal phosphorus quota, thus confirming the applicability of the model.

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