Studies on the lactoperoxidase system: reaction kinetics and antibacterial activity using two methods for hydrogen peroxide generation

1992 ◽  
Vol 72 (2) ◽  
pp. 146-153 ◽  
Author(s):  
D.A. Dionysius ◽  
P.A. Grieve ◽  
A.C. Vos
Keyword(s):  
Hydrogen Peroxide ◽  
Antibacterial Activity ◽  
Reaction Kinetics ◽  
Lactoperoxidase System ◽  
Hydrogen Peroxide Generation

Antibacterial Activity of the Lactoperoxidase System Against Listeria monocytogenes and Staphylococcus aureus in Milk

1990 ◽  
Vol 53 (12) ◽  
pp. 1010-1014 ◽  
Author(s):  
DAVID N. KAMAU ◽  
STEPHANIE DOORES ◽  
KENNETH M. PRUITT
Keyword(s):  
Hydrogen Peroxide ◽  
Staphylococcus Aureus ◽  
Growth Rate ◽  
Antibacterial Activity ◽  
Listeria Monocytogenes ◽  
Initial Period ◽  
Similar Magnitude ◽  
Lactoperoxidase System ◽  
Bactericidal Effects ◽  
And Staphylococcus Aureus

The lactoperoxidase system (LPS) was activated by addition of thiocyanate (SCN−) and hydrogen peroxide (H2O2) and utilizing the inherent milk lactoperoxidase (LP). For Listeria monocytogenes studies, initial concentrations of 2.4 mM SCN− and 0.6 mM H2O2 were added. The corresponding concentrations were 1.2 mM SCN− and 0.3 mM H2O2 for Staphylococcus aureus studies. The LPS increased the predicted time to reach half the maximum attainable CFU/ml by 326 h for L. monocytogenes at 10°C and by 6.3 h at 35°C. For S. aureus, the corresponding increases were 36 h at 10°C and 2.4 h at 37°C. During the initial period after activation of the LPS, bactericidal effects against L. monocytogenes at 35°C and S. aureus at 37°C were observed. After recovery from the effects of the LPS, growth rate of each pathogen was of similar magnitude as in the H2O2-treated and untreated milk, with the exception of L. monocytogenes at 10°C.

scholarly journals Enhanced Antibacterial Activity of Lactoperoxidase–Thiocyanate–Hydrogen Peroxide System in Reduced-Lactose Milk Whey

2019 ◽  
Vol 2019 ◽  
pp. 1-6
Author(s):  
Ahmad Ni’matullah Al-Baarri ◽  
Novia Tri Damayanti ◽  
Anang Mohamad Legowo ◽  
İsmail Hakkı Tekiner ◽  
Shigeru Hayakawa
Keyword(s):  
Hydrogen Peroxide ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Enzymatic Reaction ◽  
Bovine Milk ◽  
Milk Whey ◽  
E Coli ◽  
Lactoperoxidase System ◽  
Skimmed Milk ◽  
Derivative Products

The product of the lactoperoxidase system (LPOS) has been developed as a preservative agent to inhibit foodborne bacteria, but its action was, heretofore, limited to several original compounds in milk. This research was conducted to analyze the application of the lactoperoxidase system against Escherichia coli in fresh bovine milk and its derivative products to determine the strength of antibacterial activity. Lactoperoxidase was purified from bovine whey using the SP Sepharose Big Beads Column. The enzymatic reaction involving lactoperoxidase, thiocyanate, and hydrogen peroxide was used to generate the antibacterial agent from LPOS. This solution was then added to milk, skimmed milk, untreated whey, reduced-LPO whey, reduced-lactose whey, and high-lactose solution containing E. coli at an initial count of 6.0 log CFU/mL. LPOS showed the greatest reduction of bacteria (1.68 ± 0.1 log CFU/mL) in the reduced-lactose whey among the products tested. This result may lead to a method for enhancement of the antimicrobial activity of LPOS in milk and derived products.

Inactivation of microbiota and selected spoilage and pathogenic bacteria in milk by combinations of ultrasound, hydrogen peroxide, and active lactoperoxidase system

2016 ◽  
Vol 61 ◽  
pp. 120-125 ◽  
Author(s):  
Ahamed Kamal Shamila-Syuhada ◽  
Li-Oon Chuah ◽  
Wan Abdullah Wan-Nadiah ◽  
Lai Hoong Cheng ◽  
Abbas F.M. Alkarkhi ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Pathogenic Bacteria ◽  
Lactoperoxidase System

Hydrogen Peroxide Generation with 100% Faradaic Efficiency on Metal-Free Carbon Black

ACS Catalysis ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 2454-2459
Author(s):  
Zhe Wang ◽  
Qin-Kun Li ◽  
Chenhao Zhang ◽  
Zhihua Cheng ◽  
Weiyin Chen ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Carbon Black ◽  
Free Carbon ◽  
Faradaic Efficiency ◽  
Metal Free ◽  
Hydrogen Peroxide Generation

scholarly journals Peroxiredoxin 2 oxidation reveals hydrogen peroxide generation within erythrocytes during high-dose vitamin C administration

Redox Biology ◽  
2021 ◽  
Vol 43 ◽  
pp. 101980
Author(s):  
Andree G. Pearson ◽  
Juliet M. Pullar ◽  
John Cook ◽  
Emma S. Spencer ◽  
Margreet CM. Vissers ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Vitamin C ◽  
High Dose ◽  
Peroxiredoxin 2 ◽  
Hydrogen Peroxide Generation ◽  
High Dose Vitamin

scholarly journals Hydrogen Peroxide Generation Induces pp60srcActivation in Human Platelets

2003 ◽  
Vol 279 (3) ◽  
pp. 1665-1675 ◽  
Author(s):  
Juan A. Rosado ◽  
Pedro C. Redondo ◽  
Ginés M. Salido ◽  
Emilio Gómez-Arteta ◽  
Stewart O. Sage ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Human Platelets ◽  
Hydrogen Peroxide Generation
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