scholarly journals Enhanced Antibacterial Activity of Lactoperoxidase–Thiocyanate–Hydrogen Peroxide System in Reduced-Lactose Milk Whey

2019 ◽  
Vol 2019 ◽  
pp. 1-6
Author(s):  
Ahmad Ni’matullah Al-Baarri ◽  
Novia Tri Damayanti ◽  
Anang Mohamad Legowo ◽  
İsmail Hakkı Tekiner ◽  
Shigeru Hayakawa
Keyword(s):  
Hydrogen Peroxide ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Enzymatic Reaction ◽  
Bovine Milk ◽  
Milk Whey ◽  
E Coli ◽  
Lactoperoxidase System ◽  
Skimmed Milk ◽  
Derivative Products

The product of the lactoperoxidase system (LPOS) has been developed as a preservative agent to inhibit foodborne bacteria, but its action was, heretofore, limited to several original compounds in milk. This research was conducted to analyze the application of the lactoperoxidase system against Escherichia coli in fresh bovine milk and its derivative products to determine the strength of antibacterial activity. Lactoperoxidase was purified from bovine whey using the SP Sepharose Big Beads Column. The enzymatic reaction involving lactoperoxidase, thiocyanate, and hydrogen peroxide was used to generate the antibacterial agent from LPOS. This solution was then added to milk, skimmed milk, untreated whey, reduced-LPO whey, reduced-lactose whey, and high-lactose solution containing E. coli at an initial count of 6.0 log CFU/mL. LPOS showed the greatest reduction of bacteria (1.68 ± 0.1 log CFU/mL) in the reduced-lactose whey among the products tested. This result may lead to a method for enhancement of the antimicrobial activity of LPOS in milk and derived products.

Antibacterial Activity of Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa

2017 ◽  
Vol 8 (03) ◽  
Author(s):  
Kyoung- Sun Seo ◽  
Seong Woo Jin ◽  
Seongkyu Choi ◽  
Kyeong Won Yun
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Methanol Extract ◽  
The Other ◽  
Diffusion Method ◽  
E Coli ◽  
Agar Diffusion ◽  
Agar Diffusion Method ◽  
Crude Methanol Extract

The antibacterial activity of three Cupressaceae plants (Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa) was tested against three bacteria using the agar diffusion method. The ether and ethylacetate fraction of crude methanol extract from the three plants showed potent antibacterial activity against the tested microorganisms. The result showed that Staphylococcus aureus revealed the most sensitivity among the tested bacteria. Thujaoccidentalisether fraction and Thujaorientalis hexane fraction exhibited the highest antibacterial activity against Staphylococcus aureus. E. coli was shown the highest MIC values compared to the other two tested bacteria, which indicates the lowest antibacterial activity against the bacterium. This study promises an interesting future for designing a potentially active antibacterial agent from the three Cupressaceae plants.

scholarly journals A Comparison of Tissue Engineering Scaffolds Incorporated with Manuka Honey of Varying UMF

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Katherine R. Hixon ◽  
Tracy Lu ◽  
Sarah H. McBride-Gagyi ◽  
Blythe E. Janowiak ◽  
Scott A. Sell
Keyword(s):  
Hydrogen Peroxide ◽  
Tissue Engineering ◽  
Antibacterial Agent ◽  
Antibacterial Properties ◽  
Cell Number ◽  
Bacterial Clearance ◽  
Manuka Honey ◽  
Tissue Engineering Scaffolds ◽  
E Coli ◽  
Electrospun Scaffolds

Purpose. Manuka honey (MH) is an antibacterial agent specific to the islands of New Zealand containing both hydrogen peroxide and a Unique Manuka Factor (UMF). Although the antibacterial properties of MH have been studied, the effect of varying UMF of MH incorporated into tissue engineered scaffolds have not. Therefore, this study was designed to compare silk fibroin cryogels and electrospun scaffolds incorporated with a 5% MH concentration of various UMF.Methods. Characteristics such as porosity, bacterial clearance and adhesion, and cytotoxicity were compared.Results. Pore diameters for all cryogels were between 51 and 60 µm, while electrospun scaffolds were 10 µm. Cryogels of varying UMF displayed clearance of approximately 0.16 cm forE. coliandS. aureus. In comparison, the electrospun scaffolds clearance ranged between 0.5 and 1 cm. A glucose release of 0.5 mg/mL was observed for the first 24 hours by all scaffolds, regardless of UMF. With respect to cytotoxicity, neither scaffold caused the cell number to drop below 20,000.Conclusions. Overall, when comparing the effects of the various UMF within the two scaffolds, no significant differences were observed. This suggests that the fabricated scaffolds in this study displayed similar bacterial effects regardless of the UMF value.

scholarly journals Cinnamon Oil as an Antibacterial Agent on Natural Paper from Banana Peel (Musa paradisiaca Linn.)

2019 ◽  
Vol 4 (1) ◽  
pp. 42
Author(s):  
Widiastuti Agustina Eko Setyowati ◽  
Elfi Susanti VH ◽  
Iis Alviah ◽  
Melati Kusuma Wardani
Keyword(s):  
Antibacterial Activity ◽  
Water Content ◽  
Antibacterial Agent ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Banana Peel ◽  
Cinnamon Oil ◽  
E Coli ◽  
Musa Paradisiaca ◽  
Oil Additives

<p>The research about the use of cinnamon oil as an antibacterial agent on natural paper from Banana <em>(musa<strong> </strong>paradisiaca Linn.) </em>Peel has been conducted. The preparation of the paper using alkalization method, with the use of NaOH 4% at the temperature of 100<sup>o</sup>C for 1.5 hours. The paper is made in two variations, ie with additive cinnamon oil and without additive. The natural paper produced are tested for pH based on SNI ISO 6588-1.2010 and water content by dry-oven method based on SNI ISO 287:2010. The antibacterial activities were tested against <em>Staphylococcus aureus</em> and <em>Escherichia coli </em>using Kirby-Bauer disc diffusion method. The paper produced meet the standard of ISO 6519:2016 about Basic Paper for Laminated Plastic Wrapping Paper within the parameter of pH and water content. The results showed that the paper with additive cinnamon oil have antibacterial activity againts <em>S</em><em>.</em><em> aureus</em> and <em>E. coli </em>with inhibition diameter zone 15 mm and 13 mm respectively, whereas paper without cinnamon oil additives has no antibacterial activity against either <em>S</em><em>.</em><em> aureus</em> or <em>E. coli. </em> These result indicate that cinnamon oil can be used as an antibacterial agent on the natural paper from Banana peel <em>(musa paradisiaca </em>Linn.<em>)</em>.</p>

Antibacterial Activity of Nanoparticles Produced by Ethanol Extract of Boesenbergia rotunda Rhizome Loaded with Chitosan and Alginic Acid Against Streptococcus mutans by In Vitro

2017 ◽  
Vol 7 (03) ◽  
Author(s):  
Sri Atun ◽  
Sri Handayani ◽  
Melia Aliffiana ◽  
Hajar Nur Afifah ◽  
Anna Rakhmawati
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Methanol Extract ◽  
Alginic Acid ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
E Coli ◽  
Crude Methanol Extract

The antibacterial activity of three Cupressaceae plants (Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa) was tested against three bacteria using the agar diffusion method. The ether and ethylacetate fraction of crude methanol extract from the three plants showed potent antibacterial activity against the tested microorganisms. The result showed that Staphylococcus aureus revealed the most sensitivity among the tested bacteria. Thujaoccidentalisether fraction and Thujaorientalis hexane fraction exhibited the highest antibacterial activity against Staphylococcus aureus. E. coli was shown the highest MIC values compared to the other two tested bacteria, which indicates the lowest antibacterial activity against the bacterium. This study promises an interesting future for designing a potentially active antibacterial agent from the three Cupressaceae plants.

scholarly journals Synthesis, Characterization and Antibacterial Activity of Carbamate Derivatives of Isatin

2018 ◽  
Vol 34 (4) ◽  
pp. 2026-2030 ◽  
Author(s):  
Sarrah Sattar Jabbar
Keyword(s):  
Amino Acids ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Antibacterial Properties ◽  
E Coli ◽  
Good Antibacterial Activity ◽  
Diffusion Technique ◽  
Derivatives Of ◽  
Isatin Derivatives

In search of novel antibacterial agent, a series of new isatin derivatives (3a-d) have been synthesized by condensation isatin (2,3-indolinendione) with piperidine (hexahydropyridine), hydrazine hydrate and Boc-amino acids respectively. Compounds synthesized have been characterized by IR spectroscopy and elemental analysis. In addition, the in vitro antibacterial properties have been tested against E. coli, P. aeruginosa, and Bacillus cereus, S. aureus by employing the well diffusion technique. A majority of the synthesized compounds were showing good antibacterial activity and from comparisons of the compounds, compound 3d has been determined to be the most active compound.

Cotton Fabric Finished by WPU/LPAA Modified Nano-ZnO for Antibacterial Applications

2014 ◽  
Vol 711 ◽  
pp. 123-128
Author(s):  
Qun Li ◽  
Pei Yao Li ◽  
Xi Hui Zhao ◽  
Xiao Wen Li ◽  
Jian Ping Wang ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Antibacterial Activity ◽  
Surface Modification ◽  
Cotton Fabric ◽  
Antibacterial Agent ◽  
Antimicrobial Properties ◽  
Cotton Fabrics ◽  
E Coli ◽  
Baking Process ◽  
Fabric Surface

An antibacterial agent (ZPU) was prepared by surface modification of nanoZnO with aliphatic aqueous polyurethane (WPU) and polyacrylates sodium of lower molecular weight (LPAA). Then two kinds of cotton fabrics were dipped in ZPU and finally finished by paking-baking process. ZPU and the finished cotton fabrics were characterized by SEM, TEM and DLS. The antimicrobial properties of the cotton fabrics were investigated. The results indicated that ZnO retained nanosize with little aggregation on the fabric surface. The finished cotton fabrics showed obviously antibacterial activity againstS. aureusandE. coliwith the 24 h antibacterial rate of 99%.

Antibacterial Activity of the Lactoperoxidase System Against Listeria monocytogenes and Staphylococcus aureus in Milk

1990 ◽  
Vol 53 (12) ◽  
pp. 1010-1014 ◽  
Author(s):  
DAVID N. KAMAU ◽  
STEPHANIE DOORES ◽  
KENNETH M. PRUITT
Keyword(s):  
Hydrogen Peroxide ◽  
Staphylococcus Aureus ◽  
Growth Rate ◽  
Antibacterial Activity ◽  
Listeria Monocytogenes ◽  
Initial Period ◽  
Similar Magnitude ◽  
Lactoperoxidase System ◽  
Bactericidal Effects ◽  
And Staphylococcus Aureus

The lactoperoxidase system (LPS) was activated by addition of thiocyanate (SCN−) and hydrogen peroxide (H2O2) and utilizing the inherent milk lactoperoxidase (LP). For Listeria monocytogenes studies, initial concentrations of 2.4 mM SCN− and 0.6 mM H2O2 were added. The corresponding concentrations were 1.2 mM SCN− and 0.3 mM H2O2 for Staphylococcus aureus studies. The LPS increased the predicted time to reach half the maximum attainable CFU/ml by 326 h for L. monocytogenes at 10°C and by 6.3 h at 35°C. For S. aureus, the corresponding increases were 36 h at 10°C and 2.4 h at 37°C. During the initial period after activation of the LPS, bactericidal effects against L. monocytogenes at 35°C and S. aureus at 37°C were observed. After recovery from the effects of the LPS, growth rate of each pathogen was of similar magnitude as in the H2O2-treated and untreated milk, with the exception of L. monocytogenes at 10°C.

scholarly journals Antibacterial epoxy composites with addition of natural Artemisia annua waste

e-Polymers ◽  
2020 ◽  
Vol 20 (1) ◽  
pp. 262-271
Author(s):  
Chun Wu ◽  
Yongsi Yan ◽  
Yucheng Wang ◽  
Ping Sun ◽  
Rongrong Qi
Keyword(s):  
Escherichia Coli ◽  
Mechanical Property ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Epoxy Resins ◽  
Artemisia Annua ◽  
Antibacterial Property ◽  
Curing Temperature ◽  
Epoxy Composites ◽  
E Coli

AbstractAntibacterial epoxy resins (EP) have great potential in medical and electronic fields. During the process of extracting artemisinin from Artemisia annua, artemisia naphtha (AN) is generated as waste. The components of AN show antibacterial activity, and hence, it is introduced as a novel antibacterial agent in the epoxy matrix. In this study, the properties of epoxy resins with various AN loading were investigated. The results showed that AN/EP composites presented strong antibacterial activity against Escherichia coli and Staphylococcus aureus at the sterilization ratio of 100% against E. coli and 99.96% against S. aureus, respectively. Meanwhile, the thermal properties (curing temperature and glass transition temperature) of AN/EP composites remained well, and the mechanical property was even improved. Especially, the flexural strength of AN/EP composites could be reinforced by 62.9% when the content of AN was up to 5 wt%. For comparison, Artemisia annua powder (AAP), which was directly smashed from natural A. annua, was also mixed with epoxy resins as an antibacterial agent and showed excellent antibacterial property. Therefore, antibacterial epoxy composites containing A. annua waste as a natural resource with the enhanced mechanical property may have enormous potential in future biological and healthcare fields.

scholarly journals Antibacterial activity of CaO from blood cockle shells (Anadara granosa) calcination against Escherichia coli

2020 ◽  
Vol 21 (6) ◽  
Author(s):  
AMANDA FIRZA RUSDARYANTI ◽  
ULFAH AMALIA ◽  
SLAMET SUHARTO
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Mesh Size ◽  
Inhibition Zone ◽  
Optimal Size ◽  
E Coli ◽  
Powder Concentration ◽  
Blood Cockle ◽  
Anadara Granosa

Abstract. Rusdaryanti AF, Amalia U, Suharto S. 2020. Antibacterial activity of CaO from blood cockle shells (Anadara granosa) calcination against Escherichia coli. Biodiversitas 21: 2826-2830. Calcium carbonate (CaCO3) in blood clamp shells (BCS) was able to be converted into calcium oxide (CaO) through a calcination process. Some research stated that CaO can be used in the food industries b, one of which is as an antibacterial agent. The purpose of this study was to determine the most optimal size of BCS's powder during calcination as an antibacterial agent and its effect on the activity of Escherichia coli. Data were analyzed using analysis of variance and Kruskal-Wallis test. The results showed that 200 mesh size of BCS’s powder produced the highest yield of CaO at about 98.68% compared to 120 and 230 mesh size. The best concentration of CaO powder as an antibacterial was 3.5% with a pH of 11.3 ± 0.17. CaO powder had antibacterial activity against E. coli with minimum inhibitory concentration’s value (MIC) of-0.115; a minimum bactericidal concentration’s value (MBC) of 0 CFU/mL; inhibition zone of 3.23 ± 0.2 mm. By the PCR method, DNA degradation has occurred in E. coli cells. The differences of CaO powder concentration had significant effect (P <0.05) on the inhibition zone of E. coli.

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