Differential binding of BvgA to two classes of virulence genes of Bordetella pertussis directs promoter selectivity by RNA polymerase

1996 ◽  
Vol 21 (3) ◽  
pp. 557-565 ◽  
Author(s):  
Tao Zu ◽  
Riccardo Manetti ◽  
Rino Rappuoli ◽  
Vincenzo Scarlato
Keyword(s):  
Rna Polymerase ◽  
Bordetella Pertussis ◽  
Virulence Genes ◽  
Differential Binding

scholarly journals Overexpression of the RNA Polymerase Alpha Subunit Reduces Transcription of Bvg-Activated Virulence Genes inBordetella pertussis

2000 ◽  
Vol 182 (2) ◽  
pp. 529-531 ◽  
Author(s):  
Nicholas H. Carbonetti ◽  
Alla Romashko ◽  
Teresa J. Irish
Keyword(s):  
Rna Polymerase ◽  
Virulence Factor ◽  
Pertussis Toxin ◽  
Bordetella Pertussis ◽  
Virulence Genes ◽  
Transcriptional Activator ◽  
Alpha Subunit ◽  
Terminal Domain

ABSTRACT Overexpression of the RNA polymerase alpha subunit inBordetella pertussis reduces expression of the virulence factor pertussis toxin. Here we show that this reduction is at the level of transcription, is reversed by overexpression of the transcriptional activator BvgA, and is dependent on the C-terminal domain of alpha.

scholarly journals Control of a programmed cell death pathway in Pseudomonas aeruginosa by an antiterminator

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jennifer M. Peña ◽  
Samantha M. Prezioso ◽  
Kirsty A. McFarland ◽  
Tracy K. Kambara ◽  
Kathryn M. Ramsey ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Dna Damage ◽  
Cell Death ◽  
Programmed Cell Death ◽  
Rna Polymerase ◽  
Virulence Genes ◽  
Opportunistic Pathogen ◽  
Transcription Activator ◽  
Present Evidence ◽  
Cell Death Pathway

AbstractIn Pseudomonas aeruginosa the alp system encodes a programmed cell death pathway that is switched on in a subset of cells in response to DNA damage and is linked to the virulence of the organism. Here we show that the central regulator of this pathway, AlpA, exerts its effects by acting as an antiterminator rather than a transcription activator. In particular, we present evidence that AlpA positively regulates the alpBCDE cell lysis genes, as well as genes in a second newly identified target locus, by recognizing specific DNA sites within the promoter, then binding RNA polymerase directly and allowing it to bypass intrinsic terminators positioned downstream. AlpA thus functions in a mechanistically unusual manner to control the expression of virulence genes in this opportunistic pathogen.

The use of molecular techniques to study microbial determinants of pathogenicity

1983 ◽  
Vol 303 (1114) ◽  
pp. 219-225 ◽  
Keyword(s):  
Bordetella Pertussis ◽  
Virulence Genes ◽  
Genetic Manipulation ◽  
Enteric Bacteria ◽  
Molecular Techniques ◽  
Transfer System ◽  
Genetic Determinants ◽  
Genetic Transfer ◽  
New Methods ◽  
Considerable Success

A variety of new methods in DNA biochemistry, molecular biology and genetics have become available for the analysis of microbial determinants of pathogenicity. It has never been easier to focus upon specific genetic determinants and to manipulate them directly to meet experimental goals. Although the principles of genetic manipulation have been used with considerable success in enteric bacteria, it is not always a straightforward matter with other microorganisms. We were unable to clone Bordetella pertussis determinants of pathogenicity directly in Escherichia coli K12 by selecting for their protein products. It was possible, however, to develop a genetic transfer system and methods for the identification of specific Bordetella virulence genes. These studies not only provided the basis for the eventual successful genetic cloning of Bordetella pertussis genes but also provided an example showing that the molecular cloning of virulence genes is not always an easy task, nor even necessarily the best initial approach to take.

scholarly journals New Virulence-Activated and Virulence-Repressed Genes Identified by Systematic Gene Inactivation and Generation of Transcriptional Fusions in Bordetella pertussis

2000 ◽  
Vol 182 (20) ◽  
pp. 5902-5905 ◽  
Author(s):  
Rudy Antoine ◽  
Sylvie Alonso ◽  
Dominique Raze ◽  
Loïc Coutte ◽  
Sarah Lesjean ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Bordetella Pertussis ◽  
In Silico ◽  
Virulence Genes ◽  
Gene Inactivation ◽  
Gene Encoding ◽  
Genes Encoding ◽  
Virulence Regulator ◽  
Transcriptional Fusions

ABSTRACT An in silico scan of the partially completed genome sequence ofBordetella pertussis and analyses of transcriptional fusions generated with a new integrational vector were used to identify new potential virulence genes. The genes encoding a putative siderophore receptor, adhesins, and an autotransporter protein appeared to be regulated in a manner similar to Bordetella virulence genes by the global virulence regulator BvgAS. In contrast, the gene encoding a putative intimin-like protein appeared to be repressed under conditions of virulence.

scholarly journals Effect of mutations causing overexpression of RNA polymerase alpha subunit on regulation of virulence factors in Bordetella pertussis.

1994 ◽  
Vol 176 (23) ◽  
pp. 7267-7273 ◽  
Author(s):  
N H Carbonetti ◽  
T M Fuchs ◽  
A A Patamawenu ◽  
T J Irish ◽  
H Deppisch ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Virulence Factors ◽  
Bordetella Pertussis ◽  
Alpha Subunit

scholarly journals Surveillance of Circulating Bordetella pertussis Strains in Europe during 1998 to 2015

2018 ◽  
Vol 56 (5) ◽  
Author(s):  
Alex-Mikael Barkoff ◽  
Jussi Mertsola ◽  
Denis Pierard ◽  
Tine Dalby ◽  
Silje Vermedal Hoegh ◽  
...  
Keyword(s):  
Bordetella Pertussis ◽  
Virulence Genes ◽  
Genomic Structure ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Content Type ◽  
Present Collection ◽  
Genomic Analyses ◽  
The Common ◽  
Induced Immunity

ABSTRACT One reason for increased pertussis incidence is the adaptation of Bordetella pertussis to vaccine-induced immunity by modulating its genomic structure. This study, EUpert IV, includes 265 isolates collected from nine European countries during 2012 to 2015 ( n = 265) and compares the results to previous EUpert I to III studies (1998 to 2009). The analyses included genotyping, serotyping, pulsed-field gel electrophoresis (PFGE), and multilocus variable-number tandem-repeat analysis (MLVA). Genotyping results showed only small variations among the common virulence genes of B. pertussis . The frequencies of serotypes Fim2 and Fim3 varied among the four collections. Genomic analyses showed that MLVA type 27 increased to 80% between the periods of 1998 to 2001 and 2012 to 2015. Two PFGE profiles, BpSR3 (29.4%) and BpSR10 (27.2%), constituted more than 50% of the circulating isolates in the present collection. Our study indicates that the European B. pertussis population is changing and became more homogenous after the introduction of acellular pertussis vaccines.

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