Detection of corn stunt spiroplasma in vivo by ELISA using antisera to extracts from infected corn plants (Zea mays)

1982 ◽  
Vol 31 (4) ◽  
pp. 289-297 ◽  
Author(s):  
S.J. EDEN-GREEN
Keyword(s):  
Zea Mays ◽  
Corn Stunt ◽  
Corn Stunt Spiroplasma ◽  
Corn Plants

Defects of helicity and motility in the corn stunt spiroplasma, Spiroplasma kunkelii

1989 ◽  
Vol 35 (12) ◽  
pp. 1087-1091
Author(s):  
I.-M. Lee ◽  
R. E. Davis
Keyword(s):  
Cell Shape ◽  
Agar Medium ◽  
Polyacrylamide Gel Electrophoresis ◽  
Broth Culture ◽  
Serum Free Medium ◽  
Primary Isolation ◽  
Spiroplasma Kunkelii ◽  
Corn Stunt ◽  
Corn Stunt Spiroplasma ◽  
Corn Plants

Serum-free medium LD59 was used for primary isolation of Spiroplasma kunkelii (corn stunt spiroplasma) from diseased corn plants from Mississippi. Several strains were cloned in triplicate from one primary pure culture. Some of the strains were helical in cell shape, some were nonhelical, and some were partially helical, consisting of helical and nonhelical regions in the same cell. All helical strains except one (strain I-15) exhibited flexional and rotational motility in broth culture. Partially helical strains exhibited rotational movement in helical regions only. Nonhelical cells exhibited only flexional motility. These three morphological types produced three colony types on medium LD59 solidified with 0.8% agar. Nonhelical strains produced minute "fried egg" colonies (≤ 0.2 mm in diameter after 20 days incubation), while partially helical strains produced small colonies with granular centers surrounded by satellite colonies. Strains with normal helicity in general produced large, uniformly diffuse colonies (up to 2 mm), but one helical strain (I-15) appeared to be nonmotile in broth culture and to exhibit little translational motility in agar medium, accounting for development of minute granular colonies (about 0.2 mm in diameter). All new strains were serologically closely related to known strains of S. kunkelii, but polyacrylamide gel electrophoresis analysis of membrane proteins revealed minor differences. This is the first report of nonhelical strains of S. kunkelii and of helical strains with apparent impairment in translational motility.Key words: Spiroplasma kunkelii, nonhelical spiroplasmas, Mollicutes.

Utilization of Cogon (Imperata cylindrica L.) Silage and Urea-Treated Corn (Zea mays L.) Stover as Affected by Supplementation

2020 ◽  
Vol 24 (1) ◽  
Author(s):  
Elmar Patiga ◽  
◽  
Lolito Bestil ◽  
Hershey Mondejar ◽  
◽  
...  
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
Imperata Cylindrica

In vivo, urea-treated, silage, concentrate: Ipil-ipil supplement

scholarly journals Nitric Oxide as an Attenuator of Ecophysiological Changes in Corn (Zea mays L.) Plants Submitted to Copper Toxicity

2021 ◽  
Vol 8 (4) ◽  
pp. 735
Author(s):  
Ana Ecidia de Araújo Brito ◽  
Jessica Suellen Silva Teixeira ◽  
Diana Jhulia Palheta de Sousa ◽  
Evelyn Fátima Lima de Souza ◽  
Gabriel Ito dos Santos Teixeira ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Zea Mays ◽  
Sodium Nitroprusside ◽  
Zea Mays L ◽  
Copper Toxicity ◽  
Maximum Activity ◽  
Nitric Oxide Donor ◽  
Water Control ◽  
Ps Ii ◽  
Corn Plants

The aim of this work was to evaluate the attenuating effect of the nitric oxide donor (sodium nitroprusside-NPS) on the ecophysiological responses of corn plants (Zea mays L.) submitted to copper toxicity. The corn seeds of the K9606 VIP3 variety were soaked for 48 hours in Germitest with solution containing treatment with sodium nitroprusside Na2 [Fe (CN) 5 NO] 2H2O (0, 200 and 300µM), sodium ferrocyanide Na4Fe (CN) 6 ( 300, 100 and 0 µM) respectively and deionized water (control), sown in buckets with 15 kg of soil incubated for 50 days containing copper concentrations CuSO4.5H2O (0, 60 and 200 mg kg-1). The design consisted of randomized blocks with 12 treatments and 4 repetitions, making a total of 48 plants. in ecophysiological variables: height, leaf area and number of leaves, stem diameter increased by 32, 66% and 11.29% in the treatments with 60 mg kg-1 of copper and 200 mg kg-1 of copper, respectively. There was no effect of treatments on the chlorophyll content measured by the SPAD index and gas exchange. The chlorophyll a fluorescence variables indicate that the concentration of 200 mg kg-1 of copper caused damage to the structure of the PS II reaction center complexes and indicate a slightly protective effect of nitric oxide-NO present in sodium nitroprusside and cyanide present in sodium ferrocyanide, reflecting a smooth functioning of the maximum activity of photosystem II and the electron transport chain.

scholarly journals SKRINNING FITOKIMIA, UJI AKTIVITAS ANTIOKSIDAN DAN TABIR SURYA PADA TANAMAN JAGUNG (Zea mays L.)

PHARMACON ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 194
Author(s):  
David Albert Pangemanan ◽  
Edi Suryanto ◽  
Paulina V. Y. Yamlean
Keyword(s):  
Antioxidant Activity ◽  
Zea Mays ◽  
Zea Mays L ◽  
Total Phenolic ◽  
Flavonoid Compounds ◽  
A Value ◽  
Maximum Protection ◽  
Area North ◽  
Corn Plants ◽  
Corn Kernels

ABSTRACT Corn (Zea Mays L.) is widely developed in Indonesia. Corn kernels showed total phenolic and antioxidant activity. Antioxidants have the potential as photoprotectors, therefore, plants containing phenolic compounds can be used in the prevention of free radicals. For this reason, researcher is interested in further researching the phytochemical screening, antioxidant activity and sunscreen testing from extracts of stems, leaves, hair and leaf wrapping from corn (Zea mays L.). The samples used were corn plants (corn silk, corn leaves, corn stalks and leaf wrapping corn) originating from Kauditan area, North Minahasa. The results of the study showed that the leaf contained saponin and flavonoid compounds, the stem contained alkaloid and saponin compounds, the hair contained alkaloid and flavonoid compounds, and the leaf wrapping only contained alkaloid compounds. The highest to lowest total phenolic content values starting from the stem were 46.93 μg / mL, the leaf wrapping 37.76 μg / mL, the leaf 26.63 μg / mL and hair 14.49 μg / mL. The leaf has the highest antioxidant activity with a value of 72.81% followed by 62.87% stem then wrapping leaf 43.13%  and finally hair 29.14%. The SPF value of the stem was included in the ultra protection with a value of 16.117 followed by the leaves included in the maximum protection with an SPF value of 10.902. While the hair with SPF value of 0.6 and wrapping leaf with SPF value of 0.222 are included in the minimum protection. Keywords: Corn (Zea Mays L.), Sunscreen, Antioxidants, Phenol ABSTRAK Jagung (Zea Mays L.) banyak dikembangkan di Indonesia. Biji jagung menunjukkan kadar total fenolik dan aktivitas antioksidan. Antioksidan memiliki potensi sebagai fotoprotektor, oleh karena itu, tanaman yang mengandung senyawa fenolik dapat digunakan dalam pencegahan radikal bebas. Untuk itu peneliti tertarik untuk meneliti lebih lanjut tentang skrining fitokimia, uji aktivitas antioksidan dan tabir surya dari ekstrak batang, daun, rambut, dan daun pembungkus dari jagung (Zea mays L.). Sampel yang digunakan ialah tanaman jagung (rambut jagung, daun jagung, batang jagung dan daun pembungkus jagung) yang berasal dari daerah Kauditan, Minahasa Utara. Hasil dari penelitian menunjukkan daun mengandung senyawa saponin dan flavonoid, batang mengandung senyawa alkaloid dan saponin, rambut mengandung senyawa alkaloid dan flavonoid, dan daun pembungkus hanya mengandung senyawa alkaloid. Nilai kandungan total fenolik  yang paling tinggi sampai paling rendah dimulai dari batang sebesar 46.93 μg/mL, daun pembungkus 37.76 μg/mL, daun 26.63 μg/mL dan rambut 14.49 μg/mL. Daun memiliki aktivitas antioksidan yang paling tinggi dengan nilai 72.81% diikuti oleh batang 62.87% kemudian daun pembungkus 43.13% dan yang terakhir adalah rambut 29.14%. Nilai SPF batang termasuk dalam proteksi ultra dengan nilai 16,117 diikuti oleh daun yang termasuk dalam proteksi maksimal dengan nilai SPF 10,902. Sedangkan rambut dengan nilai SPF  0,6 dan daun pembungkus dengan nilai SPF 0,222 termasuk dalam proteksi minimal. Kata Kunci : Jagung (Zea Mays L.), Tabir Surya, Antioksidan, Fenol

scholarly journals Dosage Determination of Ethnopharmacologically Used Fermented Zea mays subsp. mays Liquor Extract of Bambusa vulgaris Schrad. Ex. J.C. Wendel Leaf Against Salmonella typhi

2020 ◽  
Vol 15 (4) ◽  
Author(s):  
Ruth Adefolakemi Gabriel-Ajobiewe ◽  
Blessing Mosope Gabriel ◽  
Mojisola Christiana Cyril-Olutayo ◽  
Felix O. Olorunmola ◽  
Kehinde Temitope Adegbehingbe
Keyword(s):  
Zea Mays ◽  
Biological Activities ◽  
Salmonella Typhi ◽  
Effective Dosage ◽  
Herbal Products ◽  
Tropical Regions ◽  
In Vivo Assay ◽  
Bamboo Leaves ◽  
Bambusa Vulgaris

Background: Bambusa vulgaris (bamboo) is a common plant in tropical regions of the world, with multiple biological activities. Cereal steep liquors have been identified to act against some fecal bacteria. Given the increasing prevalence of antimicrobial resistance coupled with the cost of antibiotics, there is a need for mitigation efforts and the shift by locals to herbal products. Methods: The factorial study design was employed. Bamboo leaves were washed, dried, milled, and soxhlet extracted for 16 h using the steep fermented liquor from Zea mays subsp. mays as the solvent. Rotary evaporated extracts of various weights were used to carry out sensitivity and minimum inhibitory concentration (MIC) tests. Oral acute toxicity was determined. The in vivo assay was used to determine the effective dosage for the treatment of infected mice while distilled water and ciprofloxacin served as negative and positive controls, respectively. Results: The average inhibition zone diameters in the sensitivity test at 25, 50, and 100 mg/mL concentrations were 16.20 ± 0.06 mm, 19.10 ± 0.02 mm, and 22.10 ± 0.05 mm, respectively, while the MIC against Salmonella typhi was 25 mg/mL. The extract was found to be safe at up to 5000 mg/kg dose, which means that the LD50 was ≥ 3808 mg/kg. The Widal test revealed that mice responded to treatment with the extract at different concentrations (50, 100, and 200 mg/mL) as the number of days increased. The in vivo assay on day 14 showed the bactericidal property at a dosage of 200 mg/mL. Conclusions: Bamboo leaves extract in steep liquor from fermented yellow maize displayed excellent pharmacological activity against the pathogenic organism of typhoid fever with a potential source of active agents.

scholarly journals Biologi Stenocranus pacificus Kirkaldy (Hemiptera: Delphacidae) pada tanaman jagung (Zea mays L.) di rumah kasa

2020 ◽  
Vol 17 (2) ◽  
pp. 104
Author(s):  
Dosma Ulina Simbolon ◽  
Maryani Cyccu Tobing ◽  
Darma Bakti
Keyword(s):  
Zea Mays ◽  
Life Cycle ◽  
Sex Ratio ◽  
Zea Mays L ◽  
Instar Nymph ◽  
Fourth Instar ◽  
The Third ◽  
First Instar ◽  
Corn Plants ◽  
North Sumatra

<p><em>Stenocranus pacificus </em>Kirkaldy (Hemiptera: Delphacidae) is destructive pest on corn plants in South Lampung and it has been reported to cause corn damages in North Sumatra. The  objective of this research was to study some aspects biology of <em>S. pacificus</em> on corn plants in screenhouse. The research was conducted by observing the biology of <em>S. pacificus</em> that was reared on corn plants in screenhouse.<em> </em>The results showed that life cycle of <em>S. pacificus </em>was 38–47 (41,60 ± 3,19) days: egg was 9–11 (10,20 ± 0,79) days, the first instar nymph was 3–4 (3,70 ± 0,48) days, the second instar nymph was 3–4 (3,90 ± 0,32) days, the third instar nymph was 3–4 (3,70 ± 0,48) days, the fourth instar nymph was 3–4 (3,80 ± 0,42) days, and the fifth instar nymph was 3–4 (3,60 ± 0,52) days. Age of female was 13–17 (15,30 ± 1,34) days. It was longer than age of male which was 8–12 (10,10 ± 1,20) days. Female could produce 181–214 (197,60 ± 11,64) eggs during its life. The sex ratio was 1:1,98.</p>

Persian clover and maize silage. III. Rumen fermentation and balance of nutrients when clover and silage are fed to lactating dairy cows

1994 ◽  
Vol 45 (8) ◽  
pp. 1783 ◽  
Author(s):  
CR Stockdale
Keyword(s):  
Zea Mays ◽  
Dairy Cows ◽  
Milk Fat ◽  
Maize Silage ◽  
Lactating Dairy Cows ◽  
Degradation Rates ◽  
Persian Clover ◽  
Yield Responses ◽  
Trifolium Resupinatum

Six rumen fistulated dairy cows, at various stages of lactation, were housed in metabolism stalls on a total of nine occasions in three experiments in which various combinations of Persian clover (Trifolium resupinatum) and maize (Zea mays) silage were offered. These experiments considered some aspects of digestion of Persian clover and Persian clover/maize silage diets in order to understand the good milk yield responses attributed to maize silage measured in previous research. Rumen ammonia concentrations and nylon bag degradation rates suggested that the cows made better use of one or both feeds when Persian clover and maize silage were offered together than they did when Persian clover was offered alone. While Persian clover and maize silage appeared to be a substantially inferior diet in terms of apparent in vivo digestibility coefficients and availability of nutrients when compared with Persian clover offered alone, milk yields were unaffected. In addition, no matter how much maize silage was offered, milk fat content remained constant. These results were attributed to an improved balance of absorbed nutrients.

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