Relationship between protamine deficiency with fertilization rate and incidence of sperm premature chromosomal condensation post-ICSI

The most rational way to reduce the eutrophic load on reservoirs-coolers of energy enterprises, in which the technological processes of energy production and growing fish products are not disrupted, is the introduction of fish-reclaimers into the reservoir. In this regard, full-system fisheries of this type include the production of fish-planting material of herbivorous species, in particular grass carp Ctenopharyngodon idella in their work plan. In the production conditions of Belovsky Fisheries LLC, a technology for growing planting material and commercial products of the white Amur has been developed, which has fully justified itself. At the same time, certain difficulties are associated with obtaining high-quality sexual products of herbivorous fish. Due to the technological features and hydrochemical regime of the reservoir, the production of plant-eating fish planting material is carried out with a high risk of over-aging of sexual products. Analysis of the results of spawning campaigns revealed a number of problems, including: zero percent of fertilization, early maturation and release of part of the eggs by females after a permissive injection, and gonad thrombosis. To solve the problem of low fertilization rate, special attention should be paid to sufficient oxygen concentration in water during pre-spawning operations with producers of Cupid. The problem of over-aging of sexual products and gonad thrombosis is proposed to be solved by using a flexible scheme of hormonal stimulation of producers.

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Restraint stress of male mice triggers apoptosis in spermatozoa and spermatogenic cells via activating the TNF-α system

Zygote ◽

10.1017/s0967199419000844 ◽

2020 ◽

Vol 28 (2) ◽

pp. 160-169 ◽

Cited By ~ 1

Author(s):

Jie Zhang ◽

De-Ling Kong ◽

Bin Xiao ◽

Hong-Jie Yuan ◽

Qiao-Qiao Kong ◽

...

Keyword(s):

Psychological Stress ◽

Restraint Stress ◽

Semen Quality ◽

Sperm Quality ◽

Fertilization Rate ◽

Seminiferous Tubules ◽

Spermatogenic Cells ◽

Male Mice ◽

Testicular Cells ◽

Tnf Α

SummaryStudies have indicated that psychological stress impairs human fertility and that various stressors can induce apoptosis of testicular cells. However, the mechanisms by which psychological stress on males reduces semen quality and stressors induce apoptosis in testicular cells are largely unclear. Using a psychological (restraint) stress mouse model, we tested whether male psychological stress triggers apoptosis of spermatozoa and spermatogenic cells through activating tumour necrosis factor (TNF)-α signalling. Wild-type or TNF-α−/− male mice were restrained for 48 h before examination for apoptosis and expression of TNF-α and TNF receptor 1 (TNFR1) in spermatozoa, epididymis, seminiferous tubules and spermatogenic cells. The results showed that male restraint significantly decreased fertilization rate and mitochondrial membrane potential, while increasing levels of malondialdehyde, active caspase-3, TNF-α and TNFR1 in spermatozoa. Male restraint also increased apoptosis and expression of TNF-α and TNFR1 in caudae epididymides, seminiferous tubules and spermatogenic cells. Sperm quality was also significantly impaired when spermatozoa were recovered 35 days after male restraint. The restraint-induced damage to spermatozoa, epididymis and seminiferous tubules was significantly ameliorated in TNF-α−/− mice. Furthermore, incubation with soluble TNF-α significantly reduced sperm motility and fertilizing potential. Taken together, the results demonstrated that male psychological stress induces apoptosis in spermatozoa and spermatogenic cells through activating the TNF-α system and that the stress-induced apoptosis in spermatogenic cells can be translated into impaired quality in future spermatozoa.

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Deleted in malignant brain tumor 1 is secreted in the oviduct and involved in the mechanism of fertilization in equine and porcine species

Reproduction ◽

10.1530/rep-13-0007 ◽

2013 ◽

Vol 146 (2) ◽

pp. 119-133 ◽

Cited By ~ 29

Author(s):

Barbara Ambruosi ◽

Gianluca Accogli ◽

Cécile Douet ◽

Sylvie Canepa ◽

Géraldine Pascal ◽

...

Keyword(s):

Brain Tumor ◽

Western Blot ◽

Immunohistochemical Analysis ◽

Fertilization Rate ◽

Malignant Brain Tumor ◽

Estrus Cycle ◽

Immunofluorescence Analysis ◽

Oviductal Fluid ◽

Porcine Spermatozoa

Oviductal environment affects preparation of gametes for fertilization, fertilization itself, and subsequent embryonic development. The aim of this study was to evaluate the effect of oviductal fluid and the possible involvement of deleted in malignant brain tumor 1 (DMBT1) on IVF in porcine and equine species that represent divergent IVF models. We first performed IVF after pre-incubation of oocytes with or without oviductal fluid supplemented or not with antibodies directed against DMBT1. We showed that oviductal fluid induces an increase in the monospermic fertilization rate and that this effect is canceled by the addition of antibodies, in both porcine and equine species. Moreover, pre-incubation of oocytes with recombinant DMBT1 induces an increase in the monospermic fertilization rate in the pig, confirming an involvement of DMBT1 in the fertilization process. The presence of DMBT1 in the oviduct at different stages of the estrus cycle was shown by western blot and confirmed by immunohistochemical analysis of ampulla and isthmus regions. The presence of DMBT1 in cumulus–oocyte complexes was shown by western blot analysis, and the localization of DMBT1 in the zona pellucida and cytoplasm of equine and porcine oocytes was observed using immunofluorescence analysis and confocal microscopy. Moreover, we showed an interaction between DMBT1 and porcine spermatozoa using surface plasmon resonance studies. Finally, a bioinformatic and phylogenetic analysis allowed us to identify the DMBT1 protein as well as a DMBT1-like protein in several mammals. Our results strongly suggest an important role of DMBT1 in the process of fertilization.

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Sperm DNA fragmentation measured by sperm chromatin dispersion impacts morphokinetic parameters, fertilization rate and blastocyst quality in ICSI treatments

Zygote ◽

10.1017/s0967199421000332 ◽

2021 ◽

pp. 1-8

Author(s):

Shikai Wang ◽

Weihong Tan ◽

Yueyue Huang ◽

Xianbao Mao ◽

Zhengda Li ◽

...

Keyword(s):

Dna Fragmentation ◽

Embryo Quality ◽

Fertilization Rate ◽

Sperm Chromatin ◽

Sperm Dna Fragmentation ◽

Blastocyst Formation ◽

High Quality ◽

Morphokinetic Parameters ◽

Sperm Dna ◽

Blastocyst Quality

Summary To determine the effects of sperm DNA fragmentation (SDF) on embryo morphokinetic parameters, cleavage patterns and embryo quality, this retrospective study analyzed 151 intracytoplasmic sperm injection (ICSI) cycles (1152 embryos collected) between November 2016 and June 2019. SDF was assessed using sperm chromatin dispersion. The cycles were divided into two groups based on the SDF rate: SDF < 15% (n = 114) and SDF ≥ 15% (n = 37). The embryo morphokinetic parameters, cleavage patterns, and embryo quality were compared between the two groups. The morphokinetic parameters tPNf, t2, t3, t4, t5, t6, and t8 were achieved significantly earlier in the SDF < 15% group compared with in the SDF ≥ 15% group. The fertilization and 2PN rates seemed to be significantly higher in the SDF < 15% group compared with in the SDF ≥ 15% group, while the abnormal cleavage rates were similar. However, a significantly higher rate of chaotic cleavage (CC) was observed in the SDF ≥ 15% group. The D3 high-quality embryo and available embryo rates were similar between the two groups. The blastocyst formation, high-quality blastocyst, and available blastocyst rates in the SDF < 15% group were significantly higher than those in the SDF ≥ 15% group. With an increase in SDF level, the chemical pregnancy, clinical pregnancy and implantation rates tended to decrease, while the miscarriage rate increased. This study demonstrated that SDF ≥ 15% reduces the fertilization rate of ICSI cycles and affects certain morphokinetic parameters. A higher SDF level can also induce a higher rate of CC, with subsequent decreases in the blastocyst formation rate and blastocyst quality.

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Fertilization and Shading Trials to Promote Pinus nigra Seedlings’ Nursery Growth under the Climate Change Demands

Sustainability ◽

10.3390/su13063563 ◽

2021 ◽

Vol 13 (6) ◽

pp. 3563

Author(s):

Marianthi Tsakaldimi ◽

Panagiota Giannaki ◽

Vladan Ivetić ◽

Nikoleta Kapsali ◽

Petros Ganatsas

Keyword(s):

Tree Species ◽

Block Design ◽

Pinus Nigra ◽

Fertilization Rate ◽

Early Growth ◽

Climate Scenarios ◽

Northern Greece ◽

Randomized Block Design ◽

Below Ground ◽

High Fertilization

Pinus nigra is one of the most widely used tree species for reforestation within its geographical distribution, as well as being a potential substitute for other tree species in Central Europe under future climate scenarios. P. nigra is transplanted into the field as two-year or three-year old seedlings because of its relatively low growth rate in the nursery. This study investigated the effects of fertilization programs and shading on P. nigra seedlings, aiming to accelerate early growth, and thus to reduce the nursery rearing time. The experiment (a completely randomized block design) was conducted in an open-air nursery by sowing seeds from Grevena, Northern Greece, in Quick pots filled with peat and perlite in a 2:1 ratio. The seedlings were subjected to two levels of fertilization—5 and 10 g L−1 NPK (30-10-10)—and two shading levels: 50% and 70%. At the ends of the first and second nursery growing season, we recorded the seedlings’ above- and below-ground morphology and biomass data. The results show that the application of all of the treatments produced seedlings which met the targeted quality standards for outplanting. However, the combination of a high fertilization rate and low shading level resulted in seedlings of a higher morphological quality, which is often considered to be an indicator for a successful seedling establishment in the field.

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Ovaprim treatment promotes oocyte development and milt fertilization rate in diploid and triploid African catfish (Clarias gariepinus)

Aquaculture International ◽

10.1007/s10499-011-9419-y ◽

2011 ◽

Vol 19 (6) ◽

pp. 1025-1034 ◽

Cited By ~ 15

Author(s):

Ali Karami ◽

Annie Christianus ◽

Hadi Zokaeifar ◽

Khairul Zamri Saad ◽

Fahmi T. J. Imraan ◽

...

Keyword(s):

Clarias Gariepinus ◽

Fertilization Rate ◽

Oocyte Development ◽

African Catfish

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Soil Carbon Dioxide Emissions from Sorghum–Sunflower Rotation in Rainfed Semi-arid Tropical Alfisols: Effects of Fertilization Rate and Legume Biomass Incorporation

Communications in Soil Science and Plant Analysis ◽

10.1080/00103624.2012.689399 ◽

2012 ◽

Vol 43 (14) ◽

pp. 1915-1929 ◽

Cited By ~ 1

Author(s):

V. Ramesh ◽

B. Venkateswarlu ◽

K. L. Sharma ◽

S. P. Wani

Keyword(s):

Carbon Dioxide ◽

Soil Carbon ◽

Carbon Dioxide Emissions ◽

Fertilization Rate ◽

Soil Carbon Dioxide ◽

Semi Arid

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111 Metsulfuron and variable nitrogen application rates on infected tall fescue: effects on stocker performance, forage yield, nutritive value, ergovaline concentration and botanical composition

Journal of Animal Science ◽

10.1093/jas/skz397.070 ◽

2020 ◽

Vol 98 (Supplement_2) ◽

pp. 31-31

Author(s):

Kevin R Meng ◽

Eric Bailey ◽

Josh Zeltwanger ◽

Hannah Allen ◽

Mikaela Adams ◽

...

Keyword(s):

Species Composition ◽

Tall Fescue ◽

Nutritive Value ◽

Ergot Alkaloids ◽

Fertilization Rate ◽

Forage Yield ◽

Nitrogen Application ◽

Forage Production ◽

Seed Head ◽

Application Rates

Abstract Chemical seed-head suppression of endophyte infected tall fescue (Lolium arundinaceum) improves stocker cattle performance but may decrease forage yield. Spring nitrogen application increases tall fescue growth with a concomitant increase in ergot alkaloids, produced by the symbiotic endophyte Epichloë coenophiala. We hypothesized that greater amounts of nitrogen applied to tall fescue would increase forage yield and offset losses in forage production from chemical suppression of seed-heads with metsulfuron without effect on alkaloid concentration. Ninety-six steers (270 ± 20 kg) were randomly assigned to one of sixteen paddocks (1.8 ha) on April 18 and continuously grazed for 57 d. Paddocks were blocked by previous use (n = 4) and randomly assigned to one of four treatments; no metsulfuron, no nitrogen (NEGCON), metsulfuron with 0 (MET0), 67 (MET67), or 134 (MET134) kg/ha of ammonium nitrate, applied March 11. Steers grazing MET0 paddocks were removed 17 d early due to insufficient forage availability. Steer weight, forage yield, forage nutritive value and ergot alkaloids in forage samples were measured monthly. Seed-head frequency and species composition were determined in June. Metsulfuron application reduced (P < 0.01) tall fescue seed-heads by 80%. Metsulfuron decreased (P = 0.03) ergovaline but ergovaline increased (P < 0.01) at each monthly sampling across treatments. Nitrogen had no impact on ergovaline concentration (P = 0.50). Forage yield tended to be least (P = 0.07) for MET0, intermediate for NEGCON and MET67, and tended to be greatest for MET134 (P = 0.08). Steer ADG was not affected by treatment (P < 0.80). Metsulfuron decreased NDF (P=0.02) regardless of fertilization rate. Forage CP increased with fertilization (P < 0.01) and no differences were detected between NEGCON and MET0 (P = 0.45). Species composition was not impacted (P >0.07) by treatment. Metsulfuron decreased seed-head growth and ergovaline concentration in tall fescue. Additional nitrogen fertilizer ameliorated forage yield lost to metsulfuron application but did not impact steer gain.

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