Glycinebetaine Accumulation, Physiological Characterizations and Growth Efficiency in Salt-tolerant and Salt-sensitive Lines of Indica Rice (Oryza sativa L. ssp. indica) in Response to Salt Stress

Salt-tolerant mutants are valuable resources for basic and applied research on plant salt tolerance. Here, we report the isolation and characterisation of a salt-tolerant rice (Oryza sativa L.) mutant. This mutant was identified from an ethyl methanesulfonate-induced Nipponbare mutant library, designated as rice salt tolerant 1 (rst1). The rst1 mutant was tolerant to salt stress and showed significantly higher shoot biomass and chlorophyll content, but lower lipid peroxidation and electrolyte leakage under NaCl stress. The improved salt tolerance of this mutant may be due mainly to its enhanced ability to restrict Na+ accumulation in shoots under salt stress conditions. Genetic analysis indicated that the salt tolerance of the rst1 mutant was controlled by a single recessive gene. Quantitative trait locus (QTL) mapping for salt tolerance was performed using an F2 population of rst1 × Peiai 64. Two QTLs were detected, in which the locus on chromosome 6 was determined to be the candidate locus of the rst1 gene. The rst1 locus was subsequently shown to reside within a 270.4-kb region defined by the markers IM29432 and IM29702. This result will be useful for map-based cloning of the rst1 gene and for marker-assisted breeding for salt tolerance in rice.

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Salt Stress Responsive Polypeptides in Germinating Seeds and Young Seedlings of Indica Rice (Oryza sativa L.)

Journal of Plant Physiology ◽

10.1016/s0176-1617(11)81696-2 ◽

1994 ◽

Vol 143 (2) ◽

pp. 250-253 ◽

Cited By ~ 7

Author(s):

U. Radha Rani ◽

A.R. Reddy

Keyword(s):

Oryza Sativa ◽

Salt Stress ◽

Oryza Sativa L ◽

Indica Rice ◽

Germinating Seeds

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In vitro characterization of salt stress effects and the selection of salt tolerant plants in rice (Oryza sativa L.)

Theoretical and Applied Genetics ◽

10.1007/bf00263275 ◽

1986 ◽

Vol 71 (5) ◽

pp. 757-760 ◽

Cited By ~ 22

Author(s):

P. Janardhan Reddy ◽

K. Vaidyanath

Keyword(s):

Oryza Sativa ◽

Salt Stress ◽

Oryza Sativa L ◽

Salt Tolerant ◽

Stress Effects ◽

Tolerant Plants ◽

In Vitro Characterization ◽

Selection Of

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Combination of traits at two developmental stages under salt stress as a measure of tolerance in a reciprocally crossed rice (Oryza sativa) population

Crop and Pasture Science ◽

10.1071/cp19560 ◽

2020 ◽

Vol 71 (4) ◽

pp. 334 ◽

Cited By ~ 2

Author(s):

Sabrina M. Elias ◽

M. Sazzadur Rahman ◽

Sumaiya F. Khan ◽

Sudip Biswas ◽

Taslima Haque ◽

...

Keyword(s):

Oryza Sativa ◽

Salt Stress ◽

Reciprocal Cross ◽

Developmental Stages ◽

Oryza Sativa L ◽

Principal Component ◽

Maturity Stage ◽

Salt Tolerant ◽

Rice Varieties ◽

Breeding Lines

The rice (Oryza sativa L.) landrace Horkuch from Bangladesh maintains efficient photosynthesis and detoxification under salt stress and was therefore considered to be a useful donor for tolerance traits. Reciprocally crossed bi-parental mapping populations were generated from salt-tolerant Horkuch and high-yielding salt-sensitive variety IR29, in order to identify superior salt-tolerant high-yielding lines as donors. The present study reports on the phenotypic screening data of ~300 F3 segregating populations from the reciprocal cross and their parental lines in seedlings and screening of a subset at maturity stage under gradual salt stress of 12 dS m–1 for seedlings and 8 dS m–1 for mature plants. Correlation, broad-sense heritability and principal component analyses for salt tolerance as well as yield-related traits were conducted in the populations at the two developmental stages. Level of salt injury was found to be correlated with traits such as filled grain weight at maturity stage and biomass-related traits at the seedling stage. This association between yield-related and survival traits helped to identify tolerant and sensitive plants, which were predicative of agronomic performance under salt stress. Moreover, use of the reciprocal-cross population showed how cytoplasmic inheritance of specific traits such as K+ concentrations can affect characteristics of donor plants. Measurement of a large number of traits and analysis of their co-inherited interrelation can therefore help identify the best performing plants under salt stress for effective breeding strategies. The data are being utilised in mapping of quantitative trait loci, and selected progenies are being used as breeding lines for producing durable salt-tolerant, high-yielding rice varieties.

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