New SNPs in the IGF2 gene and association between this gene and backfat thickness and lean meat content in Large White pigs

2006 ◽  
Vol 123 (3) ◽  
pp. 204-207 ◽  
Author(s):  
Z. Vykoukalova ◽  
A. Knoll ◽  
J. Dvorak ◽  
S. Cepica
2009 ◽  
Vol 54 (No. 10) ◽  
pp. 443-447 ◽  
Author(s):  
J. Zrůstová ◽  
A. Knoll ◽  
T. Urban ◽  
S. Čepica

Visfatin encoded by the <I>NAMPT</I> gene <I>(nicotinamide phosphoribosyltransferase,</I> formerly also known as <I>PBEF – pre-B cell colony-enhancing factor</I>) is suggested to play a role in lipid metabolism and pathophysiology of diabetes mellitus type 2. A new microsatellite <I>SCZ004</I> was detected within intron 9 of the <I>NAMPT</I> gene. In Czech Large White (<I>n</I> = 95) frequencies of alleles <I>282, 286, 287, 299</I>, and <I>304</I> were 0.02, 0.39, 0.07, 0.04, and 0.48, respectively. Allele <I>286</I> was predominant also in Landrace (<I>n</I> = 11) and Black Pied Prestice (<I>n</I> = 11) breeds. Association analysis was carried out between previously reported SNP AM999341:g.669T>C in intron 9 of the <I>NAMPT</I> gene and backfat thickness, average daily gain and lean meat content in Czech Large White (<I>n</I> = 215), Black Pied Prestice (<I>n</I> = 96) and Landrace (<I>n</I> = 105). The <I>CC</I> genotype was associated with higher backfat thickness (<I ≤ 0.01) in Black Pied Prestice, however in Czech Large White <I>CC</I> was associated with lower backfat thickness when compared to <I>TT</I> and <I>CT</I> genotypes (<I ≤ 0.05). In Czech Large White, <I>CC</I> genotype was associated with higher lean meat content when compared to <I>CT</I> (not significant) and <I>TT</I> genotype (<I ≤ 0.05) while in Landrace <I>CC</I> had the lowest lean meat content when compared to <I>CT</I> and <I>TT</I> genotypes but only the difference between <I>CC</I> and <I>CT</I>T was statistically significant (<I ≤ 0.05). In Black Pied Prestice no association with lean meat content was found. Average daily gain was not associated with the SNP in any breed.


2011 ◽  
Vol 51 (No. 6) ◽  
pp. 253-261 ◽  
Author(s):  
N. Kernerová ◽  
J. Václavovský ◽  
V. Matoušek ◽  
Z. Hanyková

The objectives of the paper were to evaluate carcass value in reared gilts on the basis of carcass analysis during the station test of purebred progeny of pigs and ultrasonically with Sonomark-100 or Piglog-105 instruments in the framework of valid methodology for performance testing, and to verify a pos-sibility of prediction of backfat thickness and/or average daily weight gain since birth and lean meat content of gilts as the elements of objectification for the evaluation of their body condition. In total 54 gilts of two breeds were evaluated: the dam breed Czech Large White and the sire breed Czech Large White &ndash; sire line. Four-parameter Richards function was used for growth evaluation. The regression function y = 63.870 &ndash; 0.447 bt<sub>1</sub> &ndash; 0.510 bt<sub>2</sub> + 0.128 MLLT was applied in ultrasonic instruments for the calculation of lean meat content. We calculated linear regression functions for the conversion of performance testing parameters (gain from birth, average backfat thickness and lean meat content) from live weight on the day of measurement per live weight declared during selections in gilts. &nbsp;


Author(s):  
Pavla Chalupová ◽  
T. Urban ◽  
A. Knoll

This research focused on recently described MspI and VspI SNPs in interleukin-18 gene and their association with selected performance traits (backfat thickness; lean meat content, average daily gain from birth, average daily gain in test, breeding value for average daily gain, for lean meat content, for reproduction and total breeding value) in a population of 344 Czech Large White sows. Both SNPs were genotyped by PCR-RFLP. In this work, these polymorphisms were associated with backfat thickness and lean meat content. Animals with genotype AA (MspI SNP) had significantly (P < 0.05) lower backfat thickness and higher lean meat content (0.77 ± 0.02 and 62.16 ± 0.23, resp.) compared to GA (0.83 ± 0.02 and 61.40 ± 0.18, resp.). Animals with genotype AA (VspI SNP) had significantly (P < 0.05) lower lean meat content and higher backfat thickness (61.10 ± 0.36 and 0.85 ± 0.03, resp.) compared to GA (62.02 ± 0.16 and 0.77 ± 0.02, resp.). No association between IL-18 polymorphisms and other performance traits was found. Our study revealed that IL-18 could be candidate gene for backfat thickness and lean meat content in pigs.


Agriculture ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 331
Author(s):  
Pavel Nevrkla ◽  
Eva Václavková ◽  
Miroslav Rozkot

The study was designed for the comparison of barrows and gilts of two contrasting genotypes in terms of growth ability, carcass value and meat quality. A total of 76 pigs (from 7 ± 1 kg to 95 ± 6 kg) of the indigenous breed Prestice Black-Pied (PB) and the commercial hybrid of Large White × Landrace × Large Whitesireline (CH) were included in this study. The groups were balanced regarding genotype and sex. Live weight at the age of 79 days (p < 0.001), live weight at slaughter (p < 0.01), average daily gain (p < 0.001), weight of carcass and left carcass side (p < 0.05) and ham proportion (p < 0.001) were higher in the CH group. The values of the given parameters were higher in barrows than in gilts. The effect of genotype and sex interaction was observed in lean meat content (p < 0.05) and backfat thickness (p < 0.01). The CH gilts showed the highest lean meat content and the lowest backfat thickness compared to the lowest values in the PB barrows. The value of pH45 (p < 0.01) and intramuscular fat content were higher in the PB group. The lightness (p < 0.001), yellowness (p < 0.01), drip loss (p < 0.05) and protein content were lower in the PB group. Higher values of lightness (p < 0.05), yellowness (p < 0.05) and cholesterol content (p < 0.05) were observed in the boars. Oxidative stability of the meat (malondialdehyde content) was more favorable in the PB pigs. The effect of genotype and sex interaction was confirmed for the pH24 value (the highest value was found in the PB barrows and the lowest in the CH barrows, p < 0.001) and for the dry matter content (lowest in the PB gilts and highest in the CH barrows, p < 0.05). Overall, the PB pigs were characterized by inferior growth and carcass parameters, however, with more favorable parameters for meat quality.


Author(s):  
Ramutis Klimas ◽  
Asta Klimienė

Genetic Trend and Relationship of Meatiness Traits of Different Breed Pigs Raised in Lithuania The purpose of this study was to determine the genetic trend of leanness for purebred pigs, and to determine the relations between meatiness traits, age and live weight of animals. The estimation of meatiness traits (backfat and loin lean thickness, lean meat percentage) of purebred Lithuanian White (LW), Large White (La.W), Yorkshire (Y), Landrace (L), Duroc (D) and Pietrain (P) pigs grown in breeding centres was evaluated by apparatus Piglog 105, during 2000-2006. In 2006, the average lean meat percentage of purebred pigs in the breeding centres of Lithuania ranged from 56.9% (LW) to 59.5% (D). During the observation period (2000-2006), the genetic trend of the leanness of LW was 6.9% (P < 0.001), La.W and L, respectively, was 2.2 and 2.5% (P < 0.01), D was 1.6% (P < 0.05), Y was 0.2% and P was 0.6%. A higher genetic trend of the leanness in the LW pig breed can be explained by import of English La.W boars. Correlation analysis showed that lean meat percentage of pigs is more related to backfat thickness (r = from -0.84 to -0.95, P < 0.001), than to loin lean thickness (r = from 0.11 to 0.30). Live weight of pigs had more influence on mentioned meatiness traits than age.


2011 ◽  
Vol 56 (No. 8) ◽  
pp. 337-344 ◽  
Author(s):  
F. Weisz ◽  
T. Urban ◽  
P. Chalupová ◽  
A. Knoll

&nbsp;In this study association analyses were performed between genes tagged SNP (IGF2, NAMPT, DGAT1, MYF4, MC3R, MC4R and MYOD1), performance traits (backfat thickness, lean meat content, average daily gain from birth to the end of the test, average daily gain in test) and estimated breeding values (EBVs) in a population of Czech Large White sows (n = 101). Genotyping of all SNPs was performed by SNapShot with the exception of SNP within NAMPT gene for which HpaII PCR-RFLP assay was used. The following significant associations between genes tagged SNPs and traits or EBVs were found out: DGAT1 &ndash; lean meat content (AG &gt; AA, P &le; 0.05), MC4R &ndash; EBV for lean meat content (GG &gt; AA, P &le; 0.05; GG &gt; AG, P &le; 0.05), IGF2 &ndash; EBV for reproduction (piglets born alive in the second and subsequent parity) (AG &gt; AA, P &le; 0.05) and total EBV (AG &gt; AA, P &le; 0.01) and MC3R &ndash; EBV for average daily gain (CT &gt; TT, P &le; 0.05).


Author(s):  
L. Sládek

The aim of this study was to establish the impact of slaughter weight, sex and the intramuscular fat (IMF) content of the musculus longissimus lumborum et thoracis (MLLT) on the carcass value of (Czech Large White x Czech Landrace) x Duroc hybrid pigs. The average lean meat content was higher in gilts (56.51 %) than in barrows (55.89 %). Average IMF content was recorded as 3.76 % in barrows and 3.42 % in gilts. The slaughter weight of the pigs did have an impact on the IMF content of the MLLT. The higher average slaughter weight (121.61 kg or 120.68 kg) of pigs intended for slaughter was established in pigs with a higher average IMF content (3.52 % or 5.20 %). Neither sex nor slaughter weight affected the monitored indicators of meat colour. The IMF content of the MLLT had no significant impact on carcass value indicators.


2002 ◽  
Vol 75 (1) ◽  
pp. 25-32 ◽  
Author(s):  
I.A.K. Youssao ◽  
V. Verleyen ◽  
P.L. Leroy

AbstractReal-time ultrasound data of backfat thickness, longissimus muscle depth and longissimus area were carried out on 335 pigs (164 gilts and 171 barrows) using the Pie Medical Scanner 200 equipped with an ASP-18 probe and 3·5 MHz to predict carcass lean content in positive stress Pietrain (TT) and negative stress Pietrain (CC or CT). They were given food ad libitum and slaughtered at an average age of 213 days and an average weight of 101 kg. The day before slaughter, longitudinal and transverse images were taken at the last rib. After slaughter, the lean meat content was estimated by a CGM (capteur gras-maigre) equipped with an 8-mm diameter Sydel probe. The carcass lean proportion was higher in homozygote TT than homozygote CC and heterozygote CT individuals (P < 0·05). Gilts had more lean meat than barrows (P < 0·05). The correlation between the lean meat proportion and ultrasound backfat thickness (UBFT) or ultrasound longissimus muscle depth (ULMD) respectively was moderate. The prediction of lean meat proportion using UBFT, ULMD and ULMA gave an R2 which varied from 0·35 to 0·79. Real-time ultrasound is a tool that could potentially be used to predict the composition of pig carcasses before slaughter particularly if measurements can be taken with a higher degree of accuracy than at present.


Author(s):  
Václav Matoušek ◽  
N. Kernerová ◽  
L. Máchal ◽  
J. Václavovský

A study was conducted in a set of hybrid gilts of F1 generation of Czech Large White x Czech Landrace breeds coming from a multiplication herd. The objective of the study was to test a possibility of using the measured traits, i.e. backfat thickness (BT) and lean meat content (LM), for objective determination of changes in the body condition score of gilts during rearing and in relation to their subsequent fertility in parities 1. At the ultrasonic measurement in the multiplication herd the purchased gilts showed the average daily gain (ADG) from birth 612.5 g, average backfat thickness (ABT) 8.34 mm, loin muscle depth (LMD) 48.83 mm and LM 62.26%. At the first determination of own performance traits in a production herd the values of ABT, LMD and LM were 13.26 mm, D 59.29 mm and 58.83%, respectively. In a subsequent measurement (at the onset of standing heat) the respective values of ABT, LMD and LM were 15.15 mm, 62.31 mm and 57.42%. Correlation coefficients for the measured traits of own performance in relation to reproductive traits in parities 1 were mostly low, statistically insignificant.


2019 ◽  
Vol 35 (2) ◽  
pp. 153-161
Author(s):  
Marija Gogic ◽  
Cedomir Radovic ◽  
Dragan Radojkovic ◽  
Radomir Savic ◽  
Maja Petricevic ◽  
...  

In the present study, the aim was to determine the impact of the following factors: age, farm, and gilt genotype, as well as the regression impact of body weight at the end of the performance test on the following tested properties: age at the end of the test/final age (FA), lifetime daily gain (LDG), the backfat thickness measured in two places (according to the Main Breeding program for Central Serbia), the depth of the long back muscle (BM) and the estimated lean meat content/meatiness (M). The study included two farms of pigs (farm 1 and farm 2), for three consecutive years (year 1, year 2 and year 3). The number of tested heads per year was 974 (year 1), 1311 (year 2) and 757 (year 3). The tested gilts were of Swedish Landrace, Large White and Duroc breeds. The gilts originated from 97 sires, while the number of daughters per sires ranged from 10 to 100. The results show that the Duroc animals were the oldest (245 days), which had the highest values for both measures of backfat thickness, but the lowest values for meatiness. In the third study year, the lowest average values were determined for the properties of the LDG, BM and M. The female animals from the farm 1 showed less growth/gain and had lower values for the estimated meatiness. As a result of the study, it was established that all included factors had a very high statistically significant influence on the variation of the tested properties (P <0.001), only the genotype of gilts showed a high statistically significant effect on the BM property (P <0.01).


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