Serum osteocalcin in donkeys as evaluated with an equine-specific radioimmunoassay

2004 ◽  
Vol 88 (1-2) ◽  
pp. 1-6 ◽  
Author(s):  
B. Carstanjen ◽  
H. Amory ◽  
I. Youssao ◽  
B. Remy
Keyword(s):  
Serum Osteocalcin ◽  
Specific Radioimmunoassay

ASSESSMENT OF RADIOIODINATED HORMONE PREPARATIONS

1969 ◽  
Vol 62 (1_Suppl) ◽  
pp. S134-S144 ◽  
Author(s):  
W. M. Hunter
Keyword(s):  
Immunological Reactivity ◽  
Specific Radioimmunoassay ◽  
Specific Activities

ABSTRACT The preparation of radioiodinated human LH and human FSH with specific activities of 50—150 μc/μg for use in specific radioimmunoassay systems is described. Methods for minimising iodination damage, for removing severely damaged fractions after iodination and for assessing the immunological reactivity of the final products are detailed. The obligation upon immunoassayists to demonstrate that their labelled preparations do indeed represent the hormones under consideration is discussed and criteria which may contribute evidence on this question are put forward.

Detection and Quantification of 7-Hydroxydehydroepiandrosterone Epimers in Three Body Fluids

2002 ◽  
Vol 67 (1) ◽  
pp. 10-18 ◽  
Author(s):  
Richard Hampl ◽  
Martin Hill ◽  
Luboslav Stárka
Keyword(s):  
Healthy Subjects ◽  
Serum Levels ◽  
Body Fluids ◽  
Gas Chromatography Mass Spectrometry ◽  
Specific Radioimmunoassay ◽  
Order Of Magnitude ◽  
Three Body ◽  
First Time ◽  
Nanomolar Range ◽  
Detection And Quantification

3β,7α-Dihydroxyandrost-5-en-17-one (1) (7α-OH-DHEA) and its 7β-hydroxy epimer 2 (7β-OH-DHEA) - 7α- and 7β-hydroxydehydroepiandrosterone - were detected and quantified in three human body fluids: in blood serum, saliva and ejaculate. Specific radioimmunoassay and gas chromatography-mass spectrometry have been used. For the first time the data on changes of these dehydroepiandrosterone metabolites are reported for a representative group of healthy subjects of both sexes (172 females and 217 males) during the life span. The serum levels of both 7-hydroxydehydroepiandrosterone epimers in serum and also in semen were in the low nanomolar range, while concentrations by one order of magnitude lower were found in saliva, but still within the detection limit. The results will serve as a basis for comparative studies of 7-hydroxydehydroepiandrosterone levels under various pathophysiological conditions, with a particular respect to autoimmune disorders.

Bone density and bone health alteration in boys with Duchenne Muscular Dystrophy: a prospective observational study

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Renu Suthar ◽  
B. V. Chaithanya Reddy ◽  
Manisha Malviya ◽  
Titiksha Sirari ◽  
Savita Verma Attri ◽  
...  
Keyword(s):  
Lumbar Spine ◽  
Bone Health ◽  
Prospective Observational Study ◽  
Bone Fractures ◽  
Long Bone ◽  
Vertebral Compression Fractures ◽  
Serum Osteocalcin ◽  
Z Score ◽  
X Ray ◽  
Compression Fractures

Abstract Objectives Boys with Duchenne Muscular Dystrophy (DMD) are at increased risk for compromised bone health, manifesting as low-impact trauma long bone fractures and vertebral compression fractures. Methods In a prospective observational study, we studied bone health parameters in North Indian boys with DMD. We consecutively enrolled ambulatory boys with DMD on glucocorticoid therapy. Bone health was evaluated with X-ray spine, Dual-energy X-ray absorptiometry (DXA), serum calcium, vitamin D3 (25[OH]D), 1,25-dihyroxyvitamin D3 (1,25[OH]2D3), serum osteocalcin, osteopontin, and N terminal telopeptide of type 1 collagen (Ntx) levels. Results A total of 76 boys with DMD were enrolled. The median age was 8.5 (interquartile range [IQR] 7.04–10.77) years. Among these, seven (9.2%) boys had long bone fractures, and four (5.3%) had vertebral compression fractures. Fifty-four (71%) boys underwent DXA scan, and among these 31 (57%) had low bone mineral density (BMD, ≤−2 z-score) at the lumbar spine. The mean BMD z-score at the lumbar spine was −2.3 (95% confidence interval [CI] = −1.8, −2.8), and at the femoral neck was −2.5 (95% CI = −2, −2.9). 25(OH)D levels were deficient in 68 (89.5%, n=76) boys, and 1,25(OH)2D3 levels were deficient in all. Mean serum osteocalcin levels were 0.68 ± 0.38 ng/mL (n=54), serum osteopontin levels were 8.6 ± 4.6 pg/mL (n=54) and serum Ntx levels were 891 ± 476 nmol/L (n=54). Boys with low BMD received glucocorticoids for longer duration, in comparison to those with normal BMD (median, IQR [16.9 (6–34) months vs. 7.8 (4.8–13.4) months]; p=0.04). Conclusions Bone health is compromised in North Indian boys with DMD. BMD at the lumbar spine is reduced in more than half of boys with DMD and nearly all had vitamin D deficiency on regular vitamin D supplements. Longer duration of glucocorticoid therapy is a risk factor for low BMD in our cohort.

scholarly journals Follicle stimulating hormone and estradiol alter immune response in osteoarthritic mice in an opposite manner

2021 ◽  
Vol 35 ◽  
pp. 205873842110161
Author(s):  
Lyudmila Belenska-Todorova ◽  
Ralitsa Zhivkova ◽  
Maya Markova ◽  
Nina Ivanovska
Keyword(s):  
Follicle Stimulating Hormone ◽  
Endocrine System ◽  
Synovial Cell ◽  
Nuclear Factor Κb ◽  
Cartilage Degradation ◽  
Osteoclast Formation ◽  
Blue Staining ◽  
Serum Osteocalcin ◽  
Tnf Α ◽  
Stimulating Hormone

Although a number of studies have shown that the occurrence and progression of osteoarthritis (OA) is related to endocrine system dysfunction, there is limited evidence about what roles sex hormones play. The aim of the present study was to examine the capacity of 17β-estradiol (ED) and follicle stimulating hormone (FSH) to alter the differentiation of bone marrow (BM) cells in arthritic mice. The experiments were conducted in collagenase-induced osteoarthritis in mice. Cartilage degradation was observed by safranin and toluidine blue staining. Flow cytometry was used to define different BM and synovial cell populations. The influence of FSH and ED on osteoclastogenesis was studied in BM cultures and on the osteoblastogenesis in primary calvarial cultures. The levels of IL-8, TNF-α, FSH, and osteocalcin were estimated by ELISA. FSH increased cartilage degradation and serum osteocalcin levels, while ED abolished it and lowered serum osteocalcin. FSH elevated the percentage of monocytoid CD14+/RANK+ and B cell CD19+/RANK+ cells in contrast to ED which inhibited the accumulation of these osteogenic populations. Also, ED changed the percentage of CD105+/F4/80+ and CD11c+ cells in the synovium. FSH augmented and ED suppressed macrophage colony-stimulating factor (M-CSF) + receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast (OC) formation, and this correlated with a respective increase and decrease of IL-8 secretion. FSH did not influence osteoblast (OB) formation while ED enhanced this process in association with changes of TNF-α, IL-8, and osteocalcin production. ED reduced osteoclast generation in bone. The key outcome of the current study is that both hormones influenced BM cell differentiation, with FSH favoring osteoclast formation and ED favoring osteoblast accumulation.

Measurement of Leukotriene Release from Rat Peritoneal Cells with a Specific Radioimmunoassay

1984 ◽  
Vol 435 (1 First Colloqu) ◽  
pp. 113-115
Author(s):  
DAVID AHARONY ◽  
PAUL DOBSON ◽  
PETER BERNSTEIN ◽  
ROBERT D. KRELL ◽  
J. BRYAN SMITH
Keyword(s):  
Peritoneal Cells ◽  
Specific Radioimmunoassay

scholarly journals Immunochemical analysis of cartilage proteoglycans. Radioimmunoassay of the molecules and the substructures

1980 ◽  
Vol 187 (3) ◽  
pp. 687-694 ◽  
Author(s):  
J Wieslander ◽  
D Heinegárd
Keyword(s):  
Hyaluronic Acid ◽  
Uronic Acid ◽  
Chondroitin Sulphate ◽  
Relative Content ◽  
Binding Region ◽  
Rich Region ◽  
Link Protein ◽  
Specific Radioimmunoassay ◽  
Cartilage Proteoglycans ◽  
Hyaluronic Acid Binding

Antibodies specifically reacting with the link proteins, the hyaluronic acid-binding region and chondroitin sulphate-peptides were used to design specific radioimmunoassay procedures. The sensitivity of the method used for the link protein was about 20 ng/ml, and the other two components could be determined at concentrations of about 2 ng/ml. The radioimmunoassay procedures were tested by using proteoglycan subfractions or fragments thereof. The procedures used to quantify link protein and hyaluronic acid-binding region showed no cross-interference. Fragments of trypsin-digested proteoglycan monomers still reacted in the radioimmunoassay for hyaluronic acid-binding region. Subfractions of proteoglycan monomers separated according to size had a gradually higher relative content of the hyaluronic acid-binding region compared with both chondroitin sulphate-peptides and uronic acid, when the molecules were smaller. The proteoglycans therefore may contain a variably large chondroitin sulphate-rich region, which has a constant substitution with polysaccharide side chains.

Validation of a specific radioimmunoassay to measure plasma cortisol in the fetal sheep

Steroids ◽  
1979 ◽  
Vol 33 (1) ◽  
pp. 45-54 ◽  
Author(s):  
David M. Magyar ◽  
John E. Buster ◽  
Carlene W. Eisner ◽  
Peter W. Nathanielsz ◽  
Rene Oliveros ◽  
...  
Keyword(s):  
Plasma Cortisol ◽  
Fetal Sheep ◽  
Specific Radioimmunoassay

The effect of levothyroxine in comparison with placebo on serum osteocalcin levels in patients with subclinical hypothyroidism

2021 ◽  
Vol 16 ◽  
Author(s):  
Reihaneh Rezaee ◽  
Masoud Mohebbi ◽  
Mozhgan Afkhamizadeh ◽  
Mohammad Ali Yaghoubi ◽  
Mona Najaf Najafi ◽  
...  
Keyword(s):  
Thyroid Hormones ◽  
Subclinical Hypothyroidism ◽  
Intervention Group ◽  
Serum Levels ◽  
Overt Hypothyroidism ◽  
Serum Osteocalcin ◽  
Significant Difference ◽  
Before And After ◽  
Metabolic Conditions ◽  
And Control

Background and Objective: Subclinical hypothyroidism can potentially develop to overt hypothyroidism. Thyroid hormones have substantial roles in metabolism and glucose homeostasis and thus are closely related to determinant factors of metabolic syndromes, such as obesity and insulin resistance. Osteocalcin is considered a predictor of metabolic conditions in thyroid diseases. This study aimed to investigate the effect of levothyroxine vs. placebo on serum osteocalcin levels in patients with subclinical hypothyroidism. Methods: This randomized clinical trial was performed on 30 patients with subclinical hypothyroidism who were referred to the endocrine clinics of Ghaem and Imam Reza hospitals in Mashhad, Iran. After giving informed consent, patients were randomly divided into intervention (50 µg/day levothyroxine for 2 months) and control (placebo) groups. Serum levels of osteocalcin, thyroid hormones, lipid profile, insulin, and fasting glucose, as well as other clinical and anthropometric data, were measured at baseline and at the end of the study. SPSS was used to analyze the data and P<0.05 was considered significant. Results: Mean age in the intervention and control groups was 35.07 ± 9.94 and 31.30 ± 4.30, respectively (P=0.20). There was no significant difference between osteocalcin levels before and after the intervention in either of the groups (P=0.54). TSH level was significantly decreased in the levothyroxine group after the intervention (P<0.01). T4 level was significantly increased in the intervention group (P=0.02). Conclusion: Levothyroxine had no significant effect on increasing the levels of serum osteocalcin in patients with subclinical hypothyroidism. We have registered the trial in the Iranian registry of clinical trials (IRCT) with the registration code IRCT20171129037677N1.

Abstract P111: Sleep duration is associated with serum osteocalcin level in an elderly Korean population: the Korean Urban and Rural Elderly (KURE) study

Circulation ◽  
2014 ◽  
Vol 129 (suppl_1) ◽  
Author(s):  
Ju-Mi Lee ◽  
Hansol Choi ◽  
Kyung Min Kim ◽  
YooSik Youm ◽  
Yumie Rhee ◽  
...  
Keyword(s):  
Bone Formation ◽  
Sleep Duration ◽  
Linear Regression Analysis ◽  
Korean Population ◽  
Health Examination ◽  
Central Research ◽  
Serum Osteocalcin ◽  
Cross Sectional ◽  
Osteocalcin Level ◽  
Serum Osteocalcin Level

Importance: Association between sleep duration and health related outcome studies have been published consistently. However there have been few studies of the association between sleep duration and bone formation in elderly. Objective: The aim of this study is to examine association between sleep duration and serum osteocalcin level in an elderly Korean population. Design: Cross sectional analysis using baseline cohort data. Setting: Community based setting Participants: Among the total of 927 participants, we excluded 1 participant because of the absence on sleep duration answer. A final total of 926 participants (624 women and 302 men aged 64-87 years) who completed baseline health examination in 2012 were enrolled in this study. Measurement: Sleep duration was measured by interviewer-assisted questionnaire. Participants answered their regular time of going to bed, time of opening eyes in the morning, and time of regular nap using open questions during the passing year. Serum osteocalcin level, a known biomarker of osteogenesis, was measured by electrochemiluminescence immunoassay method in the central research laboratory. The association between sleep duration (minutes/day) and serum osteoclacin level (ng/ml) was assessed by multiple linear regression analysis. Results: In the total population, sleep duration had significant association with serum osteocalcin level (ß=-0.008, p=0.013). One hour increase in sleep duration was associated with 0.48 ng/ml increase in serum osteocalcin level. This association remained after controlling for age, sex, body mass index, cigarette smoking, alcohol drinking, physical activity, and serum 25(OH) vitamin D (ß=0.012, p<0.001). This association was consistent before and after adjusting confounders in women (ß=-0.010, p=0.019, ß=-0.011, p=0.009, respectively) and men (ß=-0.013, p=0.012, ß=-0.013, p=0.011, respectively). Conclusions: Our findings suggest that longer sleep duration is associated with higher bone formation process in an elderly of Korean.

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