Effect of short-term water restriction in hot season on some blood parameters and immune response to Newcastle disease vaccine of local and commercial layers in the late phase of production

This trail was suggested to evaluate the amount of inherited haemagglutination inhibition (HI) antibodies for Newcastle Disease (ND) from hens to their own progeny via yolk, blood samples were collected from broiler breeders at 51st week of age aiming the collection their sera, these hens were previously vaccinated with ND-killed vaccine at the age 5 and 120 days respectively via subcutaneous route; and ND-alive vaccine at 1and 18 days then monthly intervals by aerosol, random samples of eggs were collected from panels or hatchery machines either after 24h., to detect amount and location of HI antibodies through them, day old chicks were submitted to the same protocol of blood collection as well as mothers. The results showed that the combination manner of vaccines is an ideal way of HI antibodies peak elevation, these antibodies can pass vertically from dam to progeny through yolk, and the yolk material can be used to detect the HI antibody titer by routine process.

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Study of Using Thymbra spicata leaves to reduce the toxic immunosuppresive effect of aflatoxin in broilers

The Iraqi Journal of Veterinary Medicine ◽

10.30539/iraqijvm.v32i1.774 ◽

2008 ◽

Vol 32 (1) ◽

pp. 140-147

Author(s):

Hatem M. M. Al-Naemey

Keyword(s):

Immune Response ◽

Newcastle Disease ◽

Suppressive Effect ◽

The Third ◽

The Impact ◽

Thymbra Spicata ◽

Newcastle Disease Vaccine ◽

Protection Ratio

The present study performed to evaluate the impact of addition ofThymbra spicata leaves powder of aflatoxin contaminated ration inreduction of the aflatoxin immuno suppressive effect in the immuneresponse induced by Newcastle disease vaccine in broilers.The study conducted in three groups, The first group fed aflatoxin freeration, second group fed aflatoxin contaminated ration without anytreatment, while the third group fed aflatoxin contaminated rationsupplemented of 2% Thymbra spicata leaves powder.All chick groups were vaccinated twice with Newcastle disease vaccine(NDV) at 10 and 20 days of age. ELIZA test was carried out to estimate thehumeral immune response at 6, 19 and 31 days of age. Infection challengwere done at day 32 of age with velogenic ND strain (Z-2003).The results showed that the first group has high significante antibodies titerreach to 3239±249.3 at 19days of age and 10186+845.3 at 31 days of agewith significant level 0.01 and high protection ratio against challenge(100%)at 32 days of age, while the second group has the low antibodies titerreach to 2622.6±27.3 at 19 days of age and 5712.5±786.9at 31daysof ageand low protection ratio against challenge(66.67), the third group’santibodies titer and protection ratio lower than first group and higher thansecond group ,the titers are 3060±542.5 at 19 days of age and 5712.5±786.9at31 days of age while the protection ratio against challenge is 80% at 32days of age .The results showed the immuno suppressive effect of aflatoxin as in secondgroup and the role of Thymbra spicata leaves powder in reduction of theseeffect.

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Sunflower seed oil combined with ginseng stem-leaf saponins as an adjuvant to enhance the immune response elicited by Newcastle disease vaccine in chickens

Vaccine ◽

10.1016/j.vaccine.2020.05.063 ◽

2020 ◽

Vol 38 (33) ◽

pp. 5343-5354 ◽

Cited By ~ 2

Author(s):

Lijia Yuan ◽

Yong Wang ◽

Xiaodan Ma ◽

Xuemei Cui ◽

Meiqian Lu ◽

...

Keyword(s):

Immune Response ◽

Newcastle Disease ◽

Sunflower Seed ◽

Seed Oil ◽

Sunflower Seed Oil ◽

Stem Leaf ◽

Newcastle Disease Vaccine

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Preparation of the inactivated Newcastle disease vaccine by plasma activated water and evaluation of its protection efficacy

Applied Microbiology and Biotechnology ◽

10.1007/s00253-019-10106-8 ◽

2019 ◽

Vol 104 (1) ◽

pp. 107-117 ◽

Cited By ~ 1

Author(s):

Zhou Hongzhuan ◽

Tian Ying ◽

Su Xia ◽

Guo Jinsong ◽

Zhang Zhenhua ◽

...

Keyword(s):

Immune Response ◽

Newcastle Disease ◽

Volume Ratio ◽

Enzyme Linked Immunosorbent Assay ◽

Cell Mediated Immunity ◽

Inactivated Vaccine ◽

Virus Inactivation ◽

Humoral Immune ◽

Plasma Activated Water ◽

Newcastle Disease Vaccine

AbstractVaccination has been regarded as the most effective way to reduce death and morbidity caused by infectious diseases in the livestock industry. In this study, plasma activated water (PAW) was introduced to prepare the inactivated Newcastle disease vaccine. Humoral immune response was tested by hemagglutination inhibition (HI) assay and enzyme-linked immunosorbent assay (ELISA). In addition, cell-mediated immune response was evaluated by lymphocyte proliferation assay and flow cytometry. The results demonstrated that the vaccine prepared by PAW at appropriate volume ratio could induce similar antibody titers in specific pathogen-free (SPF) chickens compared with the formaldehyde-inactivated vaccine. The challenge experiment further confirmed that the vaccine prepared by PAW conferred solid protection against virulent NDV. Moreover, it was found that the vaccine could promote the proliferation of lymphocytes and stimulate cell-mediated immunity of SPF chickens. Furthermore, analysis of electron spin resonance (ESR) spectroscopy and physicochemical properties of PAW suggested reactive oxygen and nitrogen species (RONS) played an essential role in the virus inactivation. Therefore, this study indicated that NDV treated by PAW in an appropriate ratio retained immunogenicity on the premise of virus inactivation. PAW as a promising strategy could be used to prepare inactivated vaccine for Newcastle disease.

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