scholarly journals Effect of Hatching Eggs Injection with Inactivated Newcastle Disease Vaccine and Two Doses of Cod Liver Oil on some Productive Traits and Immune Response of Broilers

2017 ◽  
Vol 5 (2) ◽  
pp. 100-106
Author(s):  
Mushtaq Talib Abdulwahid ◽  
Mashaan Abbas Abd Al-Zuhairy ◽  
Mayada Jawad Kadhum
Keyword(s):  
Immune Response ◽  
Newcastle Disease ◽  
Cod Liver Oil ◽  
Productive Traits ◽  
Hatching Eggs ◽  
Newcastle Disease Vaccine

scholarly journals Effect of injection hatching eggs with Newcastle disease vaccine and different doses of vitamin E on some productive traits and immune response of broilers

2015 ◽  
Vol 39 (2) ◽  
pp. 98-107
Author(s):  
Mushtaq T. Abdulwahid
Keyword(s):  
Immune Response ◽  
Vitamin E ◽  
Newcastle Disease ◽  
Control Group ◽  
Feed Conversion ◽  
Productive Traits ◽  
Hatching Eggs ◽  
And Control ◽  
Newcastle Disease Vaccine ◽  
Different Doses

     This study aimed to investigate the effects of injection hatching eggs with different doses of vitamin E on productive traits, some physiological and immune response to Newcastle disease vaccine. Two hundred fertile eggs of broiler breeder Ross (308) strain were incubated in automatic incubator machine after divided into four treatments (50 eggs per treatment) with two replicates. First treatment was injected 0.1ml/egg of phosphate buffer sterile into amniotic fluid at day 18th of incubation which it was considered as control group, second treatment was injected with 0.1ml/egg of inactivated Newcastle disease vaccine, third treatment was injected with 0.1ml/egg of inactivated Newcastle disease vaccine and 0.1 ml/egg of vitamin E, fourth treatment was injected with 0.1ml/egg of inactivated Newcastle disease vaccine and 0.15 ml/egg of vitamin E. All injected eggs were carried back into incubator for complete hatching process. Hatched chicks were transferred to the hall in farm of the Veterinary Medicine College/University of Baghdad; therefore, the chicks were distributed into four treatments with two replicates depending on the previously treated groups until fifth week of age. The results revealed that the treated groups with vitamin E were significantly (P≤0.05) increased in hatchability percentage, body weight, weight gain, as well as significant reduce in feed intake and improvement in feed conversion ratio as compared with second group and control, the results of third group showed significantly increased (P≤0.05) in antibody titers against Newcastle disease virus at aged (21 and 35) day as compared with the other treated groups and control.

scholarly journals Effect of injection the broiler hatching eggs with vitamin E and cod liver oil on some their productive traits and immune response to Newcastle disease vaccine

2019 ◽  
Vol 37 (2) ◽  
pp. 199-205
Author(s):  
Mushtaq T. Abdulwahid
Keyword(s):  
Vitamin E ◽  
Newcastle Disease ◽  
Broiler Chicks ◽  
Cod Liver Oil ◽  
Feed Conversion ◽  
Broiler Breeder ◽  
Treatment Groups ◽  
Antibody Titers ◽  
Productive Traits ◽  
Hatching Eggs

Four hundred fertile eggs of broiler breeder Ross strain were incubated in commercial Hatchery. Eggs were injected into amniotic fluid on 18th day of incubation after divided into four treatment groups (100 eggs per treatment) with two replicates. First treatment was injected 100 µl of PBS, second treatment was injected with100 µl of inactivated ND vaccine, the third treatment was injected with 100 µl of inactivated ND vaccine and 50 µl vitamin E (oily form) and finally the fourth treatment was injected with 100 µl of inactivated ND vaccine and 50 µl Cod liver oil. All injected eggs were carried back into hatchery for complete hatching process. Hatched chicks were transported to the farm of the Veterinary Medicine College/University of Baghdad for 42 days from 6/10/2011 to 16/11/2011. The hatched chicks from the previously treated groups were distributed into four treatments with two replicates for up to the experimental end. Results of treated groups showed significant (P ≤ 0.05) increase in body weight, weight gain, as well as significant reduce in feed intake and improvement in feed conversion ratio, also, antibody titers against Newcastle disease virus of treated groups showed significant (P ≤ 0.05) increase progressively with age until the end of the experiment compared with broiler chicks of control.

scholarly journals Effects of polysaccharide from Ophiopogon japonicus on immune response to Newcastle disease vaccine in chicken

2016 ◽  
Vol 36 (12) ◽  
pp. 1155-1159 ◽  
Author(s):  
Xu Song ◽  
Mei Cao ◽  
Zhongqiong Yin ◽  
Renyong Jia ◽  
Yuanfeng Zou ◽  
...  
Keyword(s):  
Immune Response ◽  
Newcastle Disease ◽  
Ophiopogon Japonicus ◽  
Newcastle Disease Vaccine

scholarly journals EFFECT OF VITAMIN E AND COD LIVER OIL SUPPLEMENT WITH BIVALENT OIL BASED VACCINE OF NEWCASTLE DISEASE AND INFECTIOUS BRONCHITIS DISEASE ON IMMUNE RESPONSE OF THE BROILERS.

2016 ◽  
Vol 47 (3) ◽  
Author(s):  
ABDULWAHID & et al.
Keyword(s):  
Immune Response ◽  
Vitamin E ◽  
Newcastle Disease ◽  
Challenge Test ◽  
Elisa Test ◽  
The Other ◽  
Control Group ◽  
Broiler Chicks ◽  
Cod Liver Oil ◽  
Infectious Bronchitis

This study was aimed to enhance the immune response of the broilers by vitamin E and Cod liver oil supplement with locally inactivated bivalent vaccine of Newcastle disease and Infectious bronchitis viruses. One hundred and twenty broiler chicks Ross 308 were divided to four groups at 30 chicks of each group. The 2nd group was vaccinated with 0.5ml/chick of locally inactivated ND-IB vaccine at 5 days old. The 3rd group was vaccinated with 0.5ml locally ND-IB vaccine and 0.1 ml/chick of VE. The 4th group was vaccinated with 0.5ml of locally ND-IB vaccine and 0.1ml/chick of Cod liver oil, while the first group was left without vaccination as a control group. All groups were vaccinated with attenuated ND-IB at 3 days old and attenuated IBD at 14 days old. The results of ELISA test revealed that the 3rd and 4th groups showed significantly increased (P≤0.05) in Ab titers against ND virus at aged 35 days, also, the 4th group showed significantly (P≤0.05) increased in Ab titers against IB virus at day 35 of age as compared with the other groups. The 4th group showed a significant (P≤0.05) increased in bursal index as compared with the other groups. The results of challenge test revealed that vaccinated groups resist the velogenic ND and IB viruses with relatively high productive range particularly the 4th group as compared with other groups. In conclusion, the VE and Cod liver oil supplement with local bivalent oil based vaccine of ND and IBV may enhance the immune response of the broilers particularly the 4th group which vaccinated with 0.5 ml of local bivalent oil based vaccine of ND-IB vaccine and 0.1 ml Cod liver oil.

scholarly journals Detection of the Immune Response in Broiler Breeders, and Their progeny to Newcastle Disease Vaccine

2011 ◽  
Vol 35 (1) ◽  
pp. 37-41
Author(s):  
Adil S. Ag’gar
Keyword(s):  
Immune Response ◽  
Antibody Titer ◽  
Newcastle Disease ◽  
Haemagglutination Inhibition ◽  
Blood Collection ◽  
Subcutaneous Route ◽  
Broiler Breeders ◽  
Blood Samples ◽  
Random Samples ◽  
Newcastle Disease Vaccine

This trail was suggested to evaluate the amount of inherited haemagglutination inhibition (HI) antibodies for Newcastle Disease (ND) from hens to their own progeny via yolk, blood samples were collected from broiler breeders at 51st week of age aiming the collection their sera, these hens were previously vaccinated with ND-killed vaccine at the age 5 and 120 days respectively via subcutaneous route; and ND-alive vaccine at 1and 18 days then monthly intervals by aerosol, random samples of eggs were collected from panels or hatchery machines either after 24h., to detect amount and location of HI antibodies through them, day old chicks were submitted to the same protocol of blood collection as well as mothers. The results showed that the combination manner of vaccines is an ideal way of HI antibodies peak elevation, these antibodies can pass vertically from dam to progeny through yolk, and the yolk material can be used to detect the HI antibody titer by routine process.

scholarly journals Study of Using Thymbra spicata leaves to reduce the toxic immunosuppresive effect of aflatoxin in broilers

2008 ◽  
Vol 32 (1) ◽  
pp. 140-147
Author(s):  
Hatem M. M. Al-Naemey
Keyword(s):  
Immune Response ◽  
Newcastle Disease ◽  
Suppressive Effect ◽  
The Third ◽  
The Impact ◽  
Thymbra Spicata ◽  
Newcastle Disease Vaccine ◽  
Protection Ratio

The present study performed to evaluate the impact of addition ofThymbra spicata leaves powder of aflatoxin contaminated ration inreduction of the aflatoxin immuno suppressive effect in the immuneresponse induced by Newcastle disease vaccine in broilers.The study conducted in three groups, The first group fed aflatoxin freeration, second group fed aflatoxin contaminated ration without anytreatment, while the third group fed aflatoxin contaminated rationsupplemented of 2% Thymbra spicata leaves powder.All chick groups were vaccinated twice with Newcastle disease vaccine(NDV) at 10 and 20 days of age. ELIZA test was carried out to estimate thehumeral immune response at 6, 19 and 31 days of age. Infection challengwere done at day 32 of age with velogenic ND strain (Z-2003).The results showed that the first group has high significante antibodies titerreach to 3239±249.3 at 19days of age and 10186+845.3 at 31 days of agewith significant level 0.01 and high protection ratio against challenge(100%)at 32 days of age, while the second group has the low antibodies titerreach to 2622.6±27.3 at 19 days of age and 5712.5±786.9at 31daysof ageand low protection ratio against challenge(66.67), the third group’santibodies titer and protection ratio lower than first group and higher thansecond group ,the titers are 3060±542.5 at 19 days of age and 5712.5±786.9at31 days of age while the protection ratio against challenge is 80% at 32days of age .The results showed the immuno suppressive effect of aflatoxin as in secondgroup and the role of Thymbra spicata leaves powder in reduction of theseeffect.

scholarly journals Preparation of the inactivated Newcastle disease vaccine by plasma activated water and evaluation of its protection efficacy

2019 ◽  
Vol 104 (1) ◽  
pp. 107-117 ◽  
Author(s):  
Zhou Hongzhuan ◽  
Tian Ying ◽  
Su Xia ◽  
Guo Jinsong ◽  
Zhang Zhenhua ◽  
...  
Keyword(s):  
Immune Response ◽  
Newcastle Disease ◽  
Volume Ratio ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cell Mediated Immunity ◽  
Inactivated Vaccine ◽  
Virus Inactivation ◽  
Humoral Immune ◽  
Plasma Activated Water ◽  
Newcastle Disease Vaccine

AbstractVaccination has been regarded as the most effective way to reduce death and morbidity caused by infectious diseases in the livestock industry. In this study, plasma activated water (PAW) was introduced to prepare the inactivated Newcastle disease vaccine. Humoral immune response was tested by hemagglutination inhibition (HI) assay and enzyme-linked immunosorbent assay (ELISA). In addition, cell-mediated immune response was evaluated by lymphocyte proliferation assay and flow cytometry. The results demonstrated that the vaccine prepared by PAW at appropriate volume ratio could induce similar antibody titers in specific pathogen-free (SPF) chickens compared with the formaldehyde-inactivated vaccine. The challenge experiment further confirmed that the vaccine prepared by PAW conferred solid protection against virulent NDV. Moreover, it was found that the vaccine could promote the proliferation of lymphocytes and stimulate cell-mediated immunity of SPF chickens. Furthermore, analysis of electron spin resonance (ESR) spectroscopy and physicochemical properties of PAW suggested reactive oxygen and nitrogen species (RONS) played an essential role in the virus inactivation. Therefore, this study indicated that NDV treated by PAW in an appropriate ratio retained immunogenicity on the premise of virus inactivation. PAW as a promising strategy could be used to prepare inactivated vaccine for Newcastle disease.

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