scholarly journals Adaptational changes in cellular phospholipids and fatty acid composition of the food pathogen Listeria monocytogenes as a stress response to disinfectant sanitizer benzalkonium chloride

2011 ◽  
Vol 52 (3) ◽  
pp. 275-280 ◽  
Author(s):  
P. Bisbiroulas ◽  
M. Psylou ◽  
I. Iliopoulou ◽  
I. Diakogiannis ◽  
A. Berberi ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Listeria Monocytogenes ◽  
Stress Response ◽  
Acid Composition ◽  
Benzalkonium Chloride ◽  
Food Pathogen

Influence of Altered Fatty Acid Composition on Resistance of Listeria monocytogenes to Antimicrobials

1993 ◽  
Vol 56 (4) ◽  
pp. 302-305 ◽  
Author(s):  
V. K. JUNEJA ◽  
P. M. DAVIDSON
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Listeria Monocytogenes ◽  
Acid Composition ◽  
Lipid Composition ◽  
Antimicrobial Agents ◽  
Food Preservation ◽  
Gas Liquid Chromatography ◽  
Antimicrobial Compounds

The sensitivity of Listeria monocytogenes Scott A and ATCC 19114 to antimicrobial compounds was altered when bacterial membrane lipid composition was modified by growth in the presence of added fatty acids. Analysis of cellular fatty acid composition by gas-liquid chromatography indicated that L. monocytogenes Scott A cells contained 0.97, 2.32, 0.81, and 0.72% (relative) of C14:0, C16:0, C18:0, and C18:l, respectively. In the presence of exogenously supplied C14:0, C16:0, C18:0, and C18:l, the percentages increased to 14.03, 30.92, 16.30, and 27.90%. Average MICs for L. monocytogenes Scott A and ATCC 19114 to sodium chloride, tertiary butylhydroquinone, methyl paraben, and propyl paraben were 10.0%, 81, 1406, and 544 μg/ml, respectively. Growing either strain in the presence of 50 μg/ml of either exogenously added C14:0 or C18:0 fatty acids increased their resistance to the four antimicrobial compounds. However, growth in the presence of C18:1 led to increased sensitivity to the antimicrobial agents. The results indicate that the susceptibility of L. monocytogenes to antimicrobial agents is related to the lipid composition of the cell membrane. Consequently, food preservation processes which alter fatty acid composition of L. monocytogenes could result in changes in antimicrobial susceptibility.

scholarly journals Differential Adaptive Response and Survival of Salmonella enterica Serovar Enteritidis Planktonic and Biofilm Cells Exposed to Benzalkonium Chloride

2008 ◽  
Vol 52 (10) ◽  
pp. 3669-3680 ◽  
Author(s):  
Anil K. Mangalappalli-Illathu ◽  
Sinisa Vidović ◽  
Darren R. Korber
Keyword(s):  
Surface Roughness ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Cell Surface ◽  
Acid Composition ◽  
Salmonella Enterica ◽  
Adaptive Response ◽  
Benzalkonium Chloride ◽  
Planktonic Cell ◽  
Planktonic Cells

ABSTRACT This study examined the adaptive response and survival of planktonic and biofilm phenotypes of Salmonella enterica serovar Enteritidis adapted to benzalkonium chloride (BC). Planktonic cells and biofilms were continuously exposed to 1 μg ml−1 of BC for 144 h. The proportion of BC-adapted biofilm cells able to survive a lethal BC treatment (30 μg ml−1) was significantly higher (4.6-fold) than that of BC-adapted planktonic cells. Similarly, there were 18.3-fold more survivors among the BC-adapted biofilm cells than among their nonadapted (i.e., without prior BC exposure) cell counterparts at the lethal BC concentration, and this value was significantly higher than the value for BC-adapted planktonic cells versus nonadapted cells (3.2-fold). A significantly higher (P < 0.05) proportion of surviving cells was noticed among BC-adapted biofilm cells relative to BC-adapted planktonic cells following a 10-min heat shock at 55°C. Fatty acid composition was significantly influenced by phenotype (planktonic cells or biofilm) and BC adaptation. Cell surface roughness of biofilm cells was also significantly greater (P < 0.05) than that of planktonic cells. Key proteins upregulated in BC-adapted planktonic and biofilm cells included CspA, TrxA, Tsf, YjgF, and a probable peroxidase, STY0440. Nine and 17 unique proteins were upregulated in BC-adapted planktonic and biofilm cells, respectively. These results suggest that enhanced biofilm-specific upregulation of 17 unique proteins, along with the increased expression of CspA, TrxA, Tsf, YjgF, and a probable peroxidase, phenotype-specific alterations in cell surface roughness, and a shift in fatty acid composition conferred enhanced survival to the BC-adapted biofilm cell population relative to their BC-adapted planktonic cell counterparts.

scholarly journals An integrative approach points to membrane composition as a key factor in E. coli persistence

2020 ◽  
Author(s):  
SJ Cañas-Duarte ◽  
MI Perez-Lopez ◽  
C Herrfurth ◽  
Lei Sun ◽  
LM Contreras ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Stress Response ◽  
Acid Composition ◽  
Integrative Approach ◽  
Composition Analysis ◽  
List Type ◽  
Membrane Composition ◽  
Bacterial Persistence ◽  
E Coli

SummaryIn spite of its medical importance, the genetic mechanisms of bacterial persistence, particularly spontaneous (type II) persistence, remain largely unknown. We use an integrative approach, combining mutant genome analysis, transcriptomics and lipid membrane composition analysis, to elucidate said mechanisms. In particular, we analyzed the genome of the high persistence mutant E. coli DS1 (hipQ), to identify candidate mutations responsible for the high persistence phenotype. Contrary to a recent study, we find no mutation in ydcI. We compared the expression of spontaneous persistent and growing cells using RNAseq, and find that the activation of stress response mechanisms is likely less important in spontaneous persistence than recent reports suggest. It also indicated that modifications in the cell membrane could play an important role. This hypothesis was then validated by the analysis of the fatty acid composition of persister cells of both types, which have markedly different saturation from growing cells and between each other. Taken together, our results indicate that changing membrane composition might be a key process in persistence.HighlightsRNAseq analysis of spontaneous persistence shows no evidence of stress responseIdentification of candidate SNPs for hipQ phenotype, excludes ydcIMembrane fatty acid composition is involved in both types of bacterial persistence

scholarly journals The role of FabH in Listeria monocytogenes fatty acid composition

2013 ◽  
Vol 27 (S1) ◽  
Author(s):  
Lauren P Saunders ◽  
Suranjana Sen ◽  
Brian J Wilkinson ◽  
Craig Gatto
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Listeria Monocytogenes ◽  
Acid Composition

scholarly journals Influence of Fatty Acid Precursors, Including Food Preservatives, on the Growth and Fatty Acid Composition of Listeria monocytogenes at 37 and 10�C

2010 ◽  
Vol 76 (5) ◽  
pp. 1423-1432 ◽  
Author(s):  
Mudcharee Julotok ◽  
Atul K. Singh ◽  
Craig Gatto ◽  
Brian J. Wilkinson
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Listeria Monocytogenes ◽  
Carboxylic Acid ◽  
Acid Composition ◽  
Acyl Carrier Protein ◽  
Straight Chain ◽  
Food Preservatives ◽  
Homeoviscous Adaptation ◽  
Branched Chain

ABSTRACT Listeria monocytogenes is a food-borne pathogen that grows at refrigeration temperatures and increases its content of anteiso-C15:0 fatty acid, which is believed to be a homeoviscous adaptation to ensure membrane fluidity, at these temperatures. As a possible novel approach for control of the growth of the organism, the influences of various fatty acid precursors, including branched-chain amino acids and branched- and straight-chain carboxylic acids, some of which are also well-established food preservatives, on the growth and fatty acid composition of the organism at 37�C and 10�C were studied in order to investigate whether the organism could be made to synthesize fatty acids that would result in impaired growth at low temperatures. The results indicate that the fatty acid composition of L. monocytogenes could be modulated by the feeding of branched-chain amino acid, C4, C5, and C6 branched-chain carboxylic acid, and C3 and C4 straight-chain carboxylic acid fatty acid precursors, but the growth-inhibitory effects of several preservatives were independent of effects on fatty acid composition, which were minor in the case of preservatives metabolized via acetyl coenzyme A. The ability of a precursor to modify fatty acid composition was probably a reflection of the substrate specificities of the first enzyme, FabH, in the condensation of primers of fatty acid biosynthesis with malonyl acyl carrier protein.

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