Mast cells in canine mammary gland tumour: number, distribution and EPOR positivity

Inflammatory cells have a role in tumor progression and have prognostic and therapeutic potential. The immunohistochemical expression for Mast Cell Tryptase, Macrophage Marker, CD79a, IgA, IgM and IgG on 43 cases of canine mammary gland lesions was analyzed. In hyperplasia, a few B cells (BCs) and Tumor-Associated Macrophages (TAMs) were observed, while the number of Tumor-Associated Mast Cells (TAMCs) was the highest. In the peritumoral stroma of malignant lesions, low number of TAMCs and a high number of TAMAs and BCs were present. Immune cells of each type were always lower in the intratumoral than peritumoral stroma. Positivity to CD79a was also detected in the epithelial cells of simple and micropapillay carcinomas. Immunoglobulin reactivity was mainly located in the epithelial cells where an intense positivity to IgA and IgG and a weak positivity for IgM were detectable. On the basis of our preliminary results and literature data, we suggest that such cells and molecules could be directly involved in the biology of canine mammary gland tumors. In breast cancer, stromal inflammatory cells and cancer derived immunoglobulins have been correlated with the progression, malignancy and poor prognosis of the tumor. The results herein reported show that the dog’s mammary gland epithelium also expresses immunoglobulins, and they mostly show a direct relationship with the infiltration of macrophages. In addition, this study shows that the infiltration of mast cells, B-cells and macrophages varies depending on the degree of malignancy of neoplasia.

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Expression of Tumour Endothelial Marker 8 in Canine Mammary Gland Tumour Cells

Journal of Comparative Pathology ◽

10.1016/j.jcpa.2019.10.001 ◽

2019 ◽

Vol 173 ◽

pp. 30-40

Author(s):

S. Noguchi ◽

M. Araki ◽

K. Nakajima ◽

M. Koh ◽

Y. Kokado ◽

...

Keyword(s):

Mammary Gland ◽

Tumour Cells ◽

Canine Mammary Gland ◽

Gland Tumour ◽

Endothelial Marker

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L-type amino acid transporter 1 (LAT1): A new therapeutic target for canine mammary gland tumour

The Veterinary Journal ◽

10.1016/j.tvjl.2013.06.016 ◽

2013 ◽

Vol 198 (1) ◽

pp. 164-169 ◽

Cited By ~ 6

Author(s):

Shinya Fukumoto ◽

Kiwamu Hanazono ◽

Takahiro Komatsu ◽

Hiroshi Ueno ◽

Tsuyoshi Kadosawa ◽

...

Keyword(s):

Mammary Gland ◽

Amino Acid ◽

Therapeutic Target ◽

Amino Acid Transporter ◽

Acid Transporter ◽

Canine Mammary Gland ◽

Gland Tumour

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Anti-tumour effect of metformin in canine mammary gland tumour cells

The Veterinary Journal ◽

10.1016/j.tvjl.2015.04.026 ◽

2015 ◽

Vol 205 (2) ◽

pp. 297-304 ◽

Cited By ~ 4

Author(s):

K. Saeki ◽

M. Watanabe ◽

M. Tsuboi ◽

S. Sugano ◽

R. Yoshitake ◽

...

Keyword(s):

Mammary Gland ◽

Tumour Cells ◽

Canine Mammary Gland ◽

Gland Tumour ◽

Anti Tumour Effect

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Canine pyometra associated with Bacillus species: a case report

Veterinární Medicína ◽

10.17221/36/2017-vetmed ◽

2018 ◽

Vol 63 (No. 3) ◽

pp. 143-149 ◽

Cited By ~ 2

Author(s):

MK Kim ◽

HY Yoon ◽

MH Lee ◽

JH Kim

Keyword(s):

Mammary Gland ◽

Case Report ◽

Bacillus Megaterium ◽

Fatal Outcome ◽

Bacillus Species ◽

Bacterial Isolation ◽

Sonographic Examination ◽

First Time ◽

Gland Tumour ◽

Unilateral Mastectomy

An 11-year-old, female Maltese was presented for evaluation of a mammary gland tumour. An abdominal sonographic examination showed an echogenic, fluid-filled, dilated uterus; pyometra was also diagnosed. Ovariohysterectomy and unilateral mastectomy was performed and the dog recovered uneventfully. However, 11 h later, the dog’s condition suddenly deteriorated and it died on the day after the surgery. Bacillus circulans and Bacillus megaterium were isolated from its uterine content; these species had not been reported previously in canine pyometra. The two species were resistant to various antibiotics, including cefazolin used during the treatment. We describe for the first time the presentation, diagnosis, bacterial isolation and fatal outcome of B. circulans and B. megaterium infection in a Maltese dog with pyometra.

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Fractal dimension of canine mammary gland epithelial tumors on cytologic smears

Veterinary Clinical Pathology ◽

10.1111/j.1939-165x.2006.tb00162.x ◽

2006 ◽

Vol 35 (4) ◽

pp. 446-448 ◽

Cited By ~ 12

Author(s):

Radostin Simeonov ◽

Galina Simeonova

Keyword(s):

Fractal Dimension ◽

Mammary Gland ◽

Epithelial Tumors ◽

Canine Mammary Gland

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Combined detection of CA15-3, CEA, and SF in serum and tissue of canine mammary gland tumor patients

Scientific Reports ◽

10.1038/s41598-021-85029-4 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Yuying Fan ◽

Xiaoli Ren ◽

Xuesong Liu ◽

Dongmei Shi ◽

Enshuang Xu ◽

...

Keyword(s):

Mammary Gland ◽

Malignant Tumor ◽

Reference Value ◽

Detection Sensitivity ◽

Control Group ◽

Tumor Group ◽

Increased Sensitivity ◽

Sensitivity Specificity ◽

Canine Mammary Gland ◽

Combined Detection

AbstractThe purpose of this study is to evaluate the levels and clinical diagnosis value of CA15-3, CEA, and SF in canine mammary gland tumors (CMGTs). In this study, the levels of tissues/serum CA15-3, CEA, and SF in 178 CMGT patients or healthy dogs were determined by ELISA and qRT-PCR assay. CA15-3, CEA, and SF levels of the malignant tumor group were significantly higher than that of the benign tumor group and the healthy control group. In the malignant tumor group, CA15-3 held a sensitivity of 51.8%, a specificity of 93.9%, and an accuracy of 76.8%. The sensitivity, specificity, and accuracy of CEA were 44.6%, 84.1%, and 68.1% respectively. SF held a sensitivity of 62.5%, a specificity of 85.4%, and an accuracy of 76.1%. SF showed the highest sensitivity and CA15-3 showed the highest specificity. The sensitivity, specificity, and accuracy of the combined detection of the three biomarkers in malignant tumor groups were 80.4%, 78.0%, and 80.0%, respectively, therefore combined detection increased sensitivity and accuracy but decreased specificity. In conclusion, the combined detection of serum/tissue markers CA15-3, CEA, and SF may improve the detection sensitivity of CMGTs, providing reference value for clinical application.

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