Analysis of αv integrin protein expression in human eyelid and periorbital squamous cell carcinomas

2011 ◽  
Vol 38 (7) ◽  
pp. 570-575 ◽  
Author(s):  
Adam Hsu ◽  
Bita Esmaeli ◽  
Brent Hayek ◽  
Mohammad G. Hossain ◽  
Roman Shinder ◽  
...  
Keyword(s):  
Protein Expression ◽  
Squamous Cell ◽  
Squamous Cell Carcinomas

scholarly journals Black raspberry restores the expression of the tumor suppressor p120ctn in the oral cavity of mice treated with the carcinogen dibenzo[a,l]pyrene diol epoxide

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0259998
Author(s):  
Douglas B. Stairs ◽  
Mary E. Landmesser ◽  
Cesar Aliaga ◽  
Kun-Ming Chen ◽  
Yuan-Wan Sun ◽  
...  
Keyword(s):  
Protein Expression ◽  
Squamous Cell ◽  
Immune Cells ◽  
Active Metabolite ◽  
Squamous Cell Carcinomas ◽  
Tobacco Smoke Exposure ◽  
Black Raspberry ◽  
Chemopreventive Agent ◽  
Diol Epoxide ◽  
Egfr Expression

One of the major risk factors for head and neck squamous cell carcinoma (HNSCC) is tobacco smoke exposure, but the mechanisms that can account for disease development remain to be fully defined. Utilizing our HNSCC mouse model, we analyzed oral squamous cell carcinomas (OSCC) induced by the active metabolite of a common smoke constituent, dibenzo[a,l]pyrene diol-epoxide (DBPDE). Analyzing protein expression by either immunofluorescence or immunohistochemistry, we identified biologic processes that are dysregulated in premalignant and invasive cancer lesions induced by DBPDE. Interestingly, p120ctn expression is downregulated in both stages of the disease. In addition to decreased p120ctn expression, there was also increased proliferation (as measured by Ki67), inflammation (as measured by NFkB (p65) expression), neovascularization (as measured by CD31) and recruitment of Ly6G-positive immune cells as well as strong EGFR expression. We also examined the effect of the chemopreventive agent black raspberry (BRB) on p120ctn and EGFR protein expression in DBPDE treated mice. p120ctn, but not EGFR, protein expression increased in mice treated with BRB. Our results suggest that modulation of p120ctn may, in part, account for the mechanism by which BRB inhibits DBPDE induced OSCC in mice.

Immunohistochemical evaluation of Fhit protein expression in oral squamous cell carcinomas

2007 ◽  
Vol 28 (10) ◽  
pp. 433-437 ◽  
Author(s):  
W. F. P. Heerden ◽  
T. J. P. Swart ◽  
M. B. Heerden ◽  
E. J. Rensburg ◽  
S. Engelbrecht ◽  
...  
Keyword(s):  
Protein Expression ◽  
Squamous Cell ◽  
Squamous Cell Carcinomas ◽  
Oral Squamous Cell Carcinomas ◽  
Fhit Protein

Protein expression profiles in adenocarcinomas and squamous cell carcinomas of the lung generated using tissue microarrays

2004 ◽  
Vol 203 (3) ◽  
pp. 798-807 ◽  
Author(s):  
Reinhard Ullmann ◽  
Patrizia Morbini ◽  
Iris Halbwedl ◽  
Massimo Bongiovanni ◽  
Margit Gogg-Kammerer ◽  
...  
Keyword(s):  
Protein Expression ◽  
Squamous Cell ◽  
Expression Profiles ◽  
Tissue Microarrays ◽  
Squamous Cell Carcinomas ◽  
Protein Expression Profiles

Analysis of p53 Tumor Suppressor Gene, H-ras Protooncogene and Proliferating Cell Nuclear Antigen (PCNA) in Squamous Cell Carcinomas of HRA/Skh Mice Following Exposure to 8-Methoxypsoralen (8-MOP) and UVA Radiation (PUVA Therapy)

2005 ◽  
Vol 33 (2) ◽  
pp. 292-299 ◽  
Author(s):  
Luca Lambertini ◽  
Kezia Surin ◽  
Thai-Vu T. Ton ◽  
Natasha Clayton ◽  
June K. Dunnick ◽  
...  
Keyword(s):  
Tumor Suppressor ◽  
Protein Expression ◽  
Tumor Suppressor Gene ◽  
Squamous Cell ◽  
Suppressor Gene ◽  
Squamous Cell Carcinomas ◽  
Puva Therapy ◽  
P53 Tumor Suppressor ◽  
Ras Genes ◽  
P53 Tumor Suppressor Gene

Treatment with 8-methoxypsoralen (8-MOP) and ultraviolet radiation (primarily UVA), called PUVA therapy, has been used to treat different chronic skin diseases but led to a significant increased risk for skin cancer. The National Toxicology Program (NTP) performed a study in mice treated with PUVA that showed a significant increase in squamous cell carcinomas of the skin. In the present study, we evaluated the protein expression of p53 and PCNA and DNA mutations of p53 and H-ras genes in both hyperplastic and neoplastic squamous cell lesions from the NTP study. By immunohistochemical staining, protein expression of both p53 and PCNA was detected in 3/16 (19%) of hyperplastic lesions and 14/17 (82%) of SCCs in groups treated with both 8-MOP and UVA. The mutation frequency of p53 in SCCs from mice administered 8-MOP plus UVA was 15/17 (88%) with a predominant distribution of mutations in exon 6 (14/15 – 93%). No H-ras mutations were detected in the hyperplastic lesions/tumors. The mutagenic effect of PUVA on the p53 tumor suppressor gene may lead to a conformational modification and inactivation of the p53 protein, which are considered critical steps in PUVA-induced skin carcinogenesis. The p53 mutational frequency and patterns from our study were different from those reported in human PUVA-type tumors.

Deregulated Bag-1 protein expression in human oral squamous cell carcinomas and lymph node metastases

2002 ◽  
Vol 197 (1) ◽  
pp. 60-71 ◽  
Author(s):  
Angela Hague ◽  
Graham Packham ◽  
Suzanne Huntley ◽  
Kathryn Shefford ◽  
John W. Eveson
Keyword(s):  
Lymph Node ◽  
Protein Expression ◽  
Squamous Cell ◽  
Lymph Node Metastases ◽  
Squamous Cell Carcinomas ◽  
Oral Squamous Cell Carcinomas ◽  
Node Metastases

Cyclin D1 protein expression is related to clinical progression in laryngeal squamous cell carcinomas

1997 ◽  
Vol 111 (7) ◽  
pp. 622-626 ◽  
Author(s):  
Pasquale Capaccio ◽  
Giancarlo Pruneri ◽  
Nadia Carboni ◽  
Angelo Virgilio Pagliari ◽  
Roberto Buffa ◽  
...  
Keyword(s):  
Protein Expression ◽  
Cyclin D1 ◽  
Squamous Cell ◽  
D1 Protein ◽  
Squamous Cell Carcinomas ◽  
Low Grade ◽  
Nodal Metastases ◽  
Tumour Extension ◽  
Cyclin D1 Protein ◽  
Cyclin Dl

AbstractThe expression of cyclin D1 gene was investigated in 74 laryngeal squamous cell carcinomas (LSCCs) in order to determine its clinical and prognostic value. Overexpression of cyclin Dl was detected immunohistochemically using DCS6 monoclonal antibody on formalin-fixed, paraffin-embedded tissue sections. Cyclin D1 expression was detected in 22 of the 74 cases investigated (30 per cent), thirteen of which presented nodal metastases (59 per cent); of the patients without any detectable cyclin D1 protein expression, six presented nodal metastases (12 per cent). Cyclin D1 protein expression was found in five per cent of the specimens of normal mucosa, eight per cent of thosewith low-grade dysplasia and 20 per cent of those with high-grade dysplasia. A statistically significant association was found between cyclin D1 expression and the supraglottic site (p<0.05), tumour extension (p<0.001), the presence of lymph node metastases (p<0.001), and advanced clinical stage (p<0.001). Cyclin D2 expression analysis is an important tool in the selection of LSCC patients with an aggressive clinical course.

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