Ultra-weak photon emission as a non-invasive tool for monitoring of oxidative processes in the epidermal cells of human skin: comparative study on the dorsal and the palm side of the hand

2010 ◽  
Author(s):  
Anshu Rastogi ◽  
Pavel Pospíšil
Keyword(s):  
Comparative Study ◽  
Human Skin ◽  
Photon Emission ◽  
Epidermal Cells ◽  
Non Invasive ◽  
Oxidative Processes

scholarly journals Ultraviolet A irradiation induces ultraweak photon emission with characteristic spectral patterns from biomolecules present in human skin

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Katsuhiko Tsuchida ◽  
Masaki Kobayashi
Keyword(s):  
Oxidative Stress ◽  
Human Skin ◽  
Skin Diseases ◽  
Photon Emission ◽  
Skin Tissue ◽  
Label Free ◽  
Ultraviolet A ◽  
Living Body ◽  
Non Invasive ◽  
Spectral Patterns

AbstractOxidative stress is associated with photoaging of the skin as well as with skin cancer, and is therefore, critical to monitor. Ultraweak photon emission (UPE) is extremely weak light generated during the oxidative process in the living body and has been used as a non-invasive and label-free marker for the evaluation of oxidative stress. However, the mechanism of UPE generation is not clear. Therefore, we aimed to elucidate the molecular mechanism underlying UPE generation by analyzing the spectra of UPE generated from biomolecules in the skin during ultraviolet A (UVA) exposure. The spectra of UVA-induced UPE generated from linoleic acid, linolenic acid, elastin, phospholipids, and 5,6-dihydroxyindole-2-carboxylic acid were measured, and the spectrum of human skin tissue was also obtained. The spectral patterns varied for the different biomolecules and the peaks were distinct from those of the skin tissue. These results suggested that the UPE generated from skin tissue is a collection of light emitted by biomolecules. Moreover, we proposed that UPE is generated through a photosensitization reaction and energy transfer. The identified characteristic spectral patterns of UPE can be useful to elucidate UVA-induced oxidative stress in the skin, with implications for prevention and treatment of photoaging and skin diseases.

scholarly journals Non-Invasive Label-Free Imaging of Oxidative Processes in Human Skin

2017 ◽  
Vol 112 (3) ◽  
pp. 581a
Author(s):  
Michaela Poplova ◽  
Eduard P.A. Van Wijk ◽  
Michal Cifra
Keyword(s):  
Human Skin ◽  
Label Free ◽  
Non Invasive ◽  
Oxidative Processes

Analog Circuit Failure Analysis Using Time-Resolved Emission

2005 ◽  
Author(s):  
B.J. Cain ◽  
G.L. Woods ◽  
A. Syed ◽  
R. Herlein ◽  
Toshihiro Nomura
Keyword(s):  
Analog Circuits ◽  
Integrated Circuit ◽  
Analog Circuit ◽  
Photon Emission ◽  
Nonlinear Process ◽  
Time Resolved ◽  
Non Invasive ◽  
Highly Nonlinear ◽  
Excellent Spatial Resolution ◽  
5 Ghz

Abstract Time-Resolved Emission (TRE) is a popular technique for non-invasive acquisition of time-domain waveforms from active nodes through the backside of an integrated circuit. [1] State-of-the art TRE systems offer high bandwidths (> 5 GHz), excellent spatial resolution (0.25um), and complete visibility of all nodes on the chip. TRE waveforms are typically used for detecting incorrect signal levels, race conditions, and/or timing faults with resolution of a few ps. However, extracting the exact voltage behavior from a TRE waveform is usually difficult because dynamic photon emission is a highly nonlinear process. This has limited the perceived utility of TRE in diagnosing analog circuits. In this paper, we demonstrate extraction of voltage waveforms in passing and failing conditions from a small-swing, differential logic circuit. The voltage waveforms obtained were crucial in corroborating a theory for some failures inside an 0.18um ASIC.

A STUDY OF ENDOMETRIAL THICKNESS IN TRANS VAGINAL SONOGRAPHY IN RELATION WITH HISTOPATHOLOGY REPORT IN DILATION & CURRATAGE IN AUB WOMEN

2019 ◽  
pp. 1-2
Keyword(s):  
Comparative Study ◽  
Endometrial Thickness ◽  
P Value ◽  
First Line ◽  
Non Invasive ◽  
Dilation And Curettage ◽  
Study Results ◽  
Vaginal Sonography ◽  
In Trans

A study of of endometrial thickness on TVS in relation with histopathology report on dilation and curettage. AIM AND OBJECTIVE-To set a cut off limit of endometrial thickness on TVS for differtiating between normal and abnormal endometrium. MATERIAL AND METHOD-hospital based comparative study. RESULTS-TVS is non invasive ,simple first line procedure in AUB women. Mean endometrial thickness in normal endometrial group was 8.00±2.44 mm and in abnormal endometrial group was 15.16±33 mm.The difference was found highly significant (p value<.001)

scholarly journals Flexible Potentiometric Sensor System for Non-Invasive Determination of Antioxidant Activity of Human Skin: Application for Evaluating the Effectiveness of Phytocosmetic Products

Chemosensors ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 76
Author(s):  
Aleksey V. Tarasov ◽  
Ekaterina I. Khamzina ◽  
Maria A. Bukharinova ◽  
Natalia Yu. Stozhko
Keyword(s):  
Antioxidant Activity ◽  
Human Skin ◽  
Skin Diseases ◽  
Silver Chloride ◽  
Potentiometric Sensor ◽  
Sensor System ◽  
Non Invasive ◽  
Pet Film ◽  
The Impact

In contemporary bioanalysis, monitoring the antioxidant activity (AOA) of the human skin is used to assess stresses, nutrition, cosmetics, and certain skin diseases. Non-invasive methods for skin AOA monitoring have certain advantages over invasive methods, namely cost-effectiveness, lower labor intensity, reduced risk of infection, and obtaining results in the real-time mode. This study presents a new flexible potentiometric sensor system (FPSS) for non-invasive determination of the human skin AOA, which is based on flexible film electrodes (FFEs) and membrane containing a mediator ([Fe(CN)6]3–/4–). Low-cost available materials and scalable technologies were used for FFEs manufacturing. The indicator FFE was fabricated based on polyethylene terephthalate (PET) film and carbon veil (CV) by single-sided hot lamination. The reference FFE was fabricated based on PET film and silver paint by using screen printing, which was followed by the electrodeposition of precipitate containing a mixture of silver chloride and silver ferricyanide (SCSF). The three-electrode configuration of the FPSS, including two indicator FFEs (CV/PET) and one reference FFE (SCSF/Ag/PET), has been successfully used for measuring the skin AOA and evaluating the impact of phytocosmetic products. FPSS provides reproducible (RSD ≤ 7%) and accurate (recovery of antioxidants is almost 100%) results, which allows forecasting its broad applicability in human skin AOA monitoring as well as for evaluating the effectiveness of topically and orally applied antioxidants.

scholarly journals Biological autoluminescence for assessing oxidative processes in yeast cell cultures

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Petra Vahalová ◽  
Kateřina Červinková ◽  
Michal Cifra
Keyword(s):  
Reactive Oxygen Species ◽  
Light Emission ◽  
Reactive Oxygen ◽  
Modern Medicine ◽  
Diagnostic Methods ◽  
Pathological State ◽  
Oxygen Species ◽  
Non Invasive ◽  
Oxidative Processes ◽  
Physical And Chemical

AbstractNowadays, modern medicine is looking for new, more gentle, and more efficient diagnostic methods. A pathological state of an organism is often closely connected with increased amount of reactive oxygen species. They can react with biomolecules and subsequent reactions can lead to very low endogenous light emission (biological autoluminescence—BAL). This phenomenon can be potentially used as a non-invasive and low-operational-cost tool for monitoring oxidative stress during diseases. To contribute to the understanding of the parameters affecting BAL, we analyzed the BAL from yeast Saccharomyces cerevisiae as a representative eukaryotic organism. The relationship between the BAL intensity and the amount of reactive oxygen species that originates as a result of the Fenton reaction as well as correlation between spontaneous BAL and selected physical and chemical parameters (pH, oxygen partial pressure, and cell concentration) during cell growth were established. Our results contribute to real-time non-invasive methodologies for monitoring oxidative processes in biomedicine and biotechnology.

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