Biological autoluminescence for assessing oxidative processes in yeast cell cultures

AbstractNowadays, modern medicine is looking for new, more gentle, and more efficient diagnostic methods. A pathological state of an organism is often closely connected with increased amount of reactive oxygen species. They can react with biomolecules and subsequent reactions can lead to very low endogenous light emission (biological autoluminescence—BAL). This phenomenon can be potentially used as a non-invasive and low-operational-cost tool for monitoring oxidative stress during diseases. To contribute to the understanding of the parameters affecting BAL, we analyzed the BAL from yeast Saccharomyces cerevisiae as a representative eukaryotic organism. The relationship between the BAL intensity and the amount of reactive oxygen species that originates as a result of the Fenton reaction as well as correlation between spontaneous BAL and selected physical and chemical parameters (pH, oxygen partial pressure, and cell concentration) during cell growth were established. Our results contribute to real-time non-invasive methodologies for monitoring oxidative processes in biomedicine and biotechnology.

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Biological autoluminescence for assessing oxidative processes in yeast cell cultures

10.1101/2020.11.19.388801 ◽

2020 ◽

Author(s):

Petra Vahalová ◽

Kateřina Červinková ◽

Michal Cifra

Keyword(s):

Reactive Oxygen Species ◽

Light Emission ◽

Reactive Oxygen ◽

Modern Medicine ◽

Diagnostic Methods ◽

Pathological State ◽

Oxygen Species ◽

Non Invasive ◽

Oxidative Processes ◽

Physical And Chemical

ABSTRACTNowadays, modern medicine is looking for new, more gentle, and more efficient diagnostic methods. A pathological state of an organism is often closely connected with increased amount of reactive oxygen species. They can react with biomolecules and subsequent reactions can lead to very low endogenous light emission (biological autoluminescence -BAL). This phenomenon can be potentially used as a non-invasive and low-operation-cost tool for monitoring oxidative stress during diseases. To contribute to the understanding of the parameters affecting BAL, we analyzed the BAL from yeast Saccharomyces cerevisiae as a representative eukaryotic organism. The relationship between the BAL intensity and the amount of reactive oxygen species that originates as a result of the Fenton reaction as well as correlation between spontaneous BAL and selected physical and chemical parameters (pH, oxygen partial pressure, and cell concentration) during cell growth were established. Our results contribute to real-time non-invasive methodologies for monitoring oxidative processes in biomedicine and biotechnology.

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Non-invasive Analysis of Reactive Oxygen Species Generated in Rats with Water Immersion Restraint-induced Gastric Lesions UsingIn VivoElectron Spin Resonance Spectroscopy

Free Radical Research ◽

10.1080/1071576036001641196 ◽

2004 ◽

Vol 38 (2) ◽

pp. 147-155 ◽

Cited By ~ 39

Author(s):

Keiji Yasukawa ◽

Keiko Kasazaki ◽

Fuminori Hyodo ◽

Hideo Utsumi

Keyword(s):

Reactive Oxygen Species ◽

Water Immersion ◽

Reactive Oxygen ◽

Resonance Spectroscopy ◽

Oxygen Species ◽

Gastric Lesions ◽

Non Invasive ◽

Spin Resonance

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Nanoantioxidant/Antioxidant therapy in 2019-nCoV: A new approach to reactive oxygen species mechanisms

Current Drug Therapy ◽

10.2174/1574885516666210719092931 ◽

2021 ◽

Vol 16 ◽

Author(s):

Ali Fathijouzdani ◽

Rezvan Heidarimoghadam ◽

Maryam Hazhirkamal ◽

Akram Ranjbar

Keyword(s):

Reactive Oxygen Species ◽

High Performance ◽

Membrane Lipids ◽

Reactive Oxygen ◽

Modern Medicine ◽

Multiple Organ ◽

Antioxidant Therapy ◽

Oxygen Species ◽

Medicinal Uses ◽

Organ Failures

: The COVID-19 pandemic has caused serious concerns for people around the world. The COVID-19 is associated with respiratory failure, generation of reactive oxygen species (ROS), and the lack of antioxidants among patients. Specified ROS levels have an essential role as an adjuster of immunological responses and virus cleaners. Still, excessive ROS will oxidize membrane lipids and cellular proteins and quickly destroy virus-infected cells. It can also adversely damage normal cells in the lungs and even the heart, resulting in multiple organ failures. Given the above, a highly potent antioxidant therapy can be offered to reduce cardiac loss due to COVID-19. In modern medicine, nanoparticles containing antioxidants can be used as a high-performance therapy in reducing oxidative stress in the prevention and treatment of infectious diseases. It can provide a free and interactive tool to determine whether antioxidants & nanoantioxidants can be administered for COVID-19. More research and studies are needed to investigate and make definitive opinions about their medicinal uses.

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Oxidative Processes and Paternal Contribution of Spermatozoa: Relationship Between Reactive Oxygen Species (ROS) Production and Unexplained Male Infertility

Fertility and Sterility ◽

10.1016/j.fertnstert.2005.07.185 ◽

2005 ◽

Vol 84 ◽

pp. S76-S77

Author(s):

D. Bennett ◽

M. Janes ◽

L. Sati ◽

D. Sakkas ◽

G. Huszar

Keyword(s):

Reactive Oxygen Species ◽

Male Infertility ◽

Reactive Oxygen ◽

Ros Production ◽

Oxygen Species ◽

Paternal Contribution ◽

Oxidative Processes

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Free radicals, lipid peroxidation and sperm function

Reproduction Fertility and Development ◽

10.1071/rd9950659 ◽

1995 ◽

Vol 7 (4) ◽

pp. 659 ◽

Cited By ~ 393

Author(s):

RJ Aitken

Keyword(s):

Reactive Oxygen Species ◽

Lipid Peroxidation ◽

Reactive Oxygen ◽

Human Spermatozoa ◽

Sperm Function ◽

Oxygen Species ◽

Normal Sperm ◽

Oxidative Processes ◽

Mammalian Spermatozoa

The cellular generation of reactive oxygen species was first observed in mammalian spermatozoa in the late 1940s. The field then remained dormant for 30 years until Thaddeus Mann and Roy Jones published a series of landmark papers in the 1970s in which the importance of lipid peroxidation as a mechanism for damaging mammalian spermatozoa was first intimated. The subsequent demonstration that human spermatozoa produce reactive oxygen species and are susceptible to peroxidative damage has triggered intense interest in the role of oxidative stress in the aetiology of male infertility. Moreover, data have recently been obtained to indicate that, although excessive exposure to reactive oxygen species may be harmful to spermatozoa, in physiological amounts these molecules are of importance in the control of normal sperm function. This review considers the dualistic role of reactive oxygen species and sets out the current understanding of the importance of oxidative processes in both the physiology and the pathology of the human spermatozoon. Extra keywords: human spermatozoa, reactive oxygen species.

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Non-invasive real-time imaging of reactive oxygen species (ROS) using multispectral auto-fluorescence imaging technique: a novel tool for redox biology

10.1101/2020.02.18.955112 ◽

2020 ◽

Author(s):

Abbas Habibalahi ◽

Mahdieh Dashtbani Moghari ◽

Jared M. Campbell ◽

Ayad G. Anwer ◽

Saabah B. Mahbub ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Real Time ◽

Spectral Range ◽

Imaging Technique ◽

Critical Role ◽

Reactive Oxygen ◽

Oxygen Species ◽

Redox Biology ◽

Non Invasive ◽

Entire Spectral Range

AbstractDetecting reactive oxygen species (ROS) that play a critical role as redox modulators and signalling molecules in biological systems currently requires invasive methods such as ROS - specific indicators for imaging and quantification. We developed a non-invasive, real-time, label-free imaging technique for assessing the level of ROS in live cells and thawed cryopreserved tissues that is compatible with in-vivo imaging. The technique is based on autofluorescence multispectral imaging (AFMI) carried out in an adapted fluorescence microscope with an expanded number of spectral channels spanning specific excitation (365 nm-495 nm) and emission (420 nm-700 nm) wavelength ranges. We established a strong quantitative correlation between the spectral information obtained from AFMI and the level of ROS obtained from CellROX staining. The results were obtained in several cell types (HeLa, PANC1 and mesenchymal stem cells) and in live kidney tissue. Additioanly, two spectral regimes were considered: with and without UV excitation (wavelengths > 400 nm); the latter being suitable for UV-sensitive systems such as the eye. Data were analyzed by linear regression combined with an optimization method of swarm intelligence. This allowed the calibration of AFMI signals to the level of ROS with excellent correlation (R= 0.84, p=0.00) in the entire spectral range and very good correlation (R= 0.78, p=0.00) in the limited, UV-free spectral range. We also developed a strong classifier which allowed us to distinguish moderate and high levels of ROS in these two regimes (AUC= 0.91 in the entire spectral range and AUC = 0.78 for UV-free imaging). These results indicate that ROS in cells and tissues can be imaged non-invasively, which opens the way to future clinical applications in conditions where reactive oxygen species are known to contribute to progressive disease such as in ophthalmology, diabetes, kidney disease, cancer and neurodegenerative diseases.

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Hydrogen Sulfide Prevents Formation of Reactive Oxygen Species through PI3K/Akt Signaling and Limits Ventilator-Induced Lung Injury

Oxidative Medicine and Cellular Longevity ◽

10.1155/2017/3715037 ◽

2017 ◽

Vol 2017 ◽

pp. 1-14 ◽

Cited By ~ 13

Author(s):

Sashko Georgiev Spassov ◽

Rosa Donus ◽

Paul Mikael Ihle ◽

Helen Engelstaedter ◽

Alexander Hoetzel ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Hydrogen Sulfide ◽

Lung Injury ◽

Inflammatory Responses ◽

Reactive Oxygen ◽

Akt Signaling ◽

Lung Damage ◽

Oxygen Species ◽

Ventilator Induced Lung Injury ◽

Oxidative Processes

The development of ventilator-induced lung injury (VILI) is still a major problem in mechanically ventilated patients. Low dose inhalation of hydrogen sulfide (H2S) during mechanical ventilation has been proven to prevent lung damage by limiting inflammatory responses in rodent models. However, the capacity of H2S to affect oxidative processes in VILI and its underlying molecular signaling pathways remains elusive. In the present study we show that ventilation with moderate tidal volumes of 12 ml/kg for 6 h led to an excessive formation of reactive oxygen species (ROS) in mice lungs which was prevented by supplemental inhalation of 80 parts per million of H2S. In addition, phosphorylation of the signaling protein Akt was induced by H2S. In contrast, inhibition of Akt by LY294002 during ventilation reestablished lung damage, neutrophil influx, and proinflammatory cytokine release despite the presence of H2S. Moreover, the ability of H2S to induce the antioxidant glutathione and to prevent ROS production was reversed in the presence of the Akt inhibitor. Here, we provide the first evidence that H2S-mediated Akt activation is a key step in protection against VILI, suggesting that Akt signaling limits not only inflammatory but also detrimental oxidative processes that promote the development of lung injury.

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Biomedical Applications of Reactive Oxygen Species Generation by Metal Nanoparticles

Materials ◽

10.3390/ma14010053 ◽

2020 ◽

Vol 14 (1) ◽

pp. 53

Author(s):

Roberto Canaparo ◽

Federica Foglietta ◽

Tania Limongi ◽

Loredana Serpe

Keyword(s):

Reactive Oxygen Species ◽

Materials Science ◽

Reactive Oxygen Species Generation ◽

Chemical Properties ◽

Reactive Oxygen ◽

Oxygen Species ◽

Design Synthesis ◽

Wide Range ◽

Therapeutic Procedures ◽

Physical And Chemical

The design, synthesis and characterization of new nanomaterials represents one of the most dynamic and transversal aspects of nanotechnology applications in the biomedical field. New synthetic and engineering improvements allow the design of a wide range of biocompatible nanostructured materials (NSMs) and nanoparticles (NPs) which, with or without additional chemical and/or biomolecular surface modifications, are more frequently employed in applications for successful diagnostic, drug delivery and therapeutic procedures. Metal-based nanoparticles (MNPs) including metal NPs, metal oxide NPs, quantum dots (QDs) and magnetic NPs, thanks to their physical and chemical properties have gained much traction for their functional use in biomedicine. In this review it is highlighted how the generation of reactive oxygen species (ROS), which in many respects could be considered a negative aspect of the interaction of MNPs with biological matter, may be a surprising nanotechnology weapon. From the exchange of knowledge between branches such as materials science, nanotechnology, engineering, biochemistry and medicine, researchers and clinicians are setting and standardizing treatments by tuning ROS production to induce cancer or microbial cell death.

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