Serological Evidence of Leishmania donovani Infection in Apparently Healthy Dogs using Direct Agglutination Test (DAT) and rk39 Dipstick Tests in Kafta Humera, north-west Ethiopia

2011 ◽  
Vol 58 (3) ◽  
pp. 255-262 ◽  
Author(s):  
S. Kalayou ◽  
H. Tadelle ◽  
A. Bsrat ◽  
N. Abebe ◽  
M. Haileselassie ◽  
...  
Keyword(s):  
Leishmania Donovani ◽  
Agglutination Test ◽  
Direct Agglutination Test ◽  
Serological Evidence ◽  
North West ◽  
North West Ethiopia ◽  
Healthy Dogs ◽  
Apparently Healthy

scholarly journals Performance of an indigenous β-mercaptoethanol-modified antigen in comparison with a commercial reference in direct agglutination test for detection of canine visceral leishmaniasis

2014 ◽  
Vol 63 (1) ◽  
pp. 106-110
Author(s):  
Saul José Semião-Santos ◽  
Laura Barral Veloso ◽  
Paulo Paes de Andrade ◽  
Marcia Almeida de Melo ◽  
Luis Miguel Lourenço Martins ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Leishmania Infantum ◽  
Leishmania Donovani ◽  
Agglutination Test ◽  
Canine Visceral Leishmaniasis ◽  
Direct Agglutination Test ◽  
Serum Samples

We compared the performance of a locally produced β-mercaptoethanol-modified promastigote antigen (β-ME-Ag) of an indigenous Leishmania infantum strain against that of a trypsinized Leishmania donovani reference (REF-Ag) in the direct agglutination test (DAT) for detection of canine visceral leishmaniasis (CVL). One hundred and fifty-one serum samples collected from dogs belonging to four groups with different conditions were included. At a DAT titre of 1 : 320, statistically determined as optimal cut-off value for β-ME-Ag, and 1 : 160 for REF-Ag, a sensitivity and a specificity of 100 % were estimated for β-ME-Ag in comparison with 96.6 % and 100 %, respectively, for REF-Ag. Overall, levels of agglutination titres recorded for the two antigens were highly concordant (Cohen’s κ = 0.879) in both the CVL and non-CVL groups. Based on current results, and ease experienced in processing the antigen and reading the test outcome, we recommend incorporation of β-ME-Ag in DAT for confirmation or exclusion of suspected CVL in dogs.

scholarly journals Use of a Newly Developed β-Mercaptoethanol Enzyme-Linked Immunosorbent Assay To Diagnose Visceral Leishmaniasis in Patients in Eastern Sudan

2007 ◽  
Vol 14 (12) ◽  
pp. 1592-1595 ◽  
Author(s):  
Durria Mansour ◽  
Elfadil M. Abass ◽  
Mohamed el Mutasim ◽  
Abdelhafeiz Mahamoud ◽  
Abdallah el Harith
Keyword(s):  
Visceral Leishmaniasis ◽  
Immunosorbent Assay ◽  
Leishmania Donovani ◽  
Clinical Evidence ◽  
High Reliability ◽  
Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Direct Agglutination Test ◽  
Symptomatic Group ◽  
Eastern Sudan

ABSTRACT Corroboration of serology results is essential for restricting the risk of inappropriate antileishmanial prescription. A direct agglutination test (DAT) and a recently developed β-mercaptoethanol-modified enzyme-linked immunosorbent assay (β-ME ELISA) based on the use of antigen prepared as described for the DAT were applied to 416 sera from two Sudanese populations with and without clinical evidence of visceral leishmaniasis (VL). Of 285 sera with the lowest antileishmanial DAT titers (≤1:100 to 1:1,600), 270 (94.7%) scored comparable minimum β-ME ELISA absorbance values (≤0.1 to 0.26). In 117 sera that demonstrated the highest DAT titers (1:12,800 to ≥1:25,600), 86 (73.5%) scored maximum (0.81 to ≥1.35) and 30 (25.6%) medium (0.27 to 0.80) β-ME ELISA absorbance values. VL diagnosis was established for 142 (44.1%) patients in the VL-symptomatic group (n = 322), based on positive microscopy for Leishmania donovani in lymph node aspirates or positive DAT (titer, ≥1:3,200). Of the 125 sera from the symptomatic patients for whom microscopy was positive for VL, 111 (88.8%) had comparable positive DAT and β-ME ELISA readings. In all 17 sera from the symptomatic DAT-positive patients for whom leishmaniasis was not established by microscopy but who responded favorably to antileishmanial therapy, absorbance values (≥0.27) indicative of VL were obtained by β-ME ELISA. Of 197 symptomatic patients for whom microscopy was negative for VL, 172 (87.3%) tested negative in β-ME ELISA and 180 (91.4%) in DAT. Based on the high reliability demonstrated here for VL detection, β-ME ELISA fulfills the requirement of confirming DAT results in patients manifesting suspected VL.

scholarly journals Potential of Direct Agglutination Test Based on Promastigote and Amastigote Antigens for Serodiagnosis of Post-Kala-Azar Dermal Leishmaniasis

2005 ◽  
Vol 12 (10) ◽  
pp. 1191-1194 ◽  
Author(s):  
Ruchi Singh ◽  
B. V. Subba Raju ◽  
R. K. Jain ◽  
Poonam Salotra
Keyword(s):  
Sensitivity And Specificity ◽  
Leishmania Donovani ◽  
Parasite Burden ◽  
Agglutination Test ◽  
Field Conditions ◽  
Antibody Detection ◽  
Direct Agglutination Test ◽  
Kala Azar ◽  
Potential Applicability ◽  
Strip Test

ABSTRACT Post-kala-azar dermal leishmaniasis (PKDL) is a dermal complication, a sequel to kala-azar. Diagnosis of PKDL presents a challenge due to the low parasite burden in the lesions. The direct agglutination test (DAT) based on promastigote and amastigote antigens of Leishmania donovani of indigenous isolates was developed to diagnose PKDL, and the results were compared with those of the rk39 strip test. The sensitivities of DAT for antileishmanial antibody detection, based on promastigote and amastigote antigens at a cutoff titer of 1:800 were 98.5% and 100%, respectively, with corresponding specificities of 96.5% and 100%. DAT could correctly detect 100% polymorphic cases and 95.4% macular PKDL cases. In comparison, the rk39 strip test was able to correctly diagnose 95.6% of polymorphic and 86.0% macular PKDL cases. DAT based on axenic amastigote antigen provided 100% sensitivity and specificity, making it particularly useful for macular PKDL cases, which are often missed by the rk39 strip test. Thus, DAT provides a simple, reliable, and inexpensive test for PKDL diagnosis with potential applicability in field conditions.

scholarly journals Serological markers for Leishmania donovani infection in Nepal: agreement between direct agglutination test and rK39 ELISA

2010 ◽  
Vol 15 (11) ◽  
pp. 1390-1394 ◽  
Author(s):  
Basudha Khanal ◽  
Suman Rijal ◽  
Bart Ostyn ◽  
Albert Picado ◽  
Kamlesh Gidwani ◽  
...  
Keyword(s):  
Leishmania Donovani ◽  
Agglutination Test ◽  
Direct Agglutination Test ◽  
Serological Markers

Successful substitution of fetal calf serum by human plasma for bulk cultivation of Leishmania donovani promastigotes

2013 ◽  
Vol 62 (8) ◽  
pp. 1165-1169 ◽  
Author(s):  
Abdelhafeiz Mahamoud ◽  
Hussam Ali Osman ◽  
Durria Mansour ◽  
Abdallah el Harith
Keyword(s):  
Visceral Leishmaniasis ◽  
Human Plasma ◽  
Leishmania Donovani ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Diagnostic Procedures ◽  
Agglutination Test ◽  
Direct Agglutination Test ◽  
Standard Medium ◽  
Liver Infusion Tryptose

The potential of human plasma (HP) or serum (HS) as a replacement for fetal calf serum (FCS) was evaluated in a liver infusion tryptose (LIT) medium for bulk cultivation of Leishmania donovani promastigotes. The promastigote yield with the LIT-FCS standard medium was 0.4–1.8×107 ml−1, and yields of 0.5–3.4×107 (P = 0.527) and 0.4–2.4×107 (P = 0.062) were recorded for two LIT medium variants containing HP or HS as supplement instead of FCS. Significantly, higher promastigote yields of 1.3–4.9×107 ml−1 were demonstrated when LIT medium was supplemented with HP of blood group O but not A, B, AB or equally pooled ABO (P = 0.007–0.020). Matching (P = 0.56) strong positive (1 : 10 2400 to ≥1 : 262 144 00) and weak negative (1 : 5–1 : 160) direct agglutination test (DAT) titres, respectively, were demonstrated in 24 visceral leishmaniasis (VL) and 45 non-VL sera for both standard LIT-FCS and alternative LIT-HP derived antigens. Our findings indicate strong potential for sustainable production of promastigotes for important diagnostic procedures such as DAT in the VL affected areas.

scholarly journals Persistence ofLeishmania donovaniAntibodies in Past Visceral Leishmaniasis Cases in India

2010 ◽  
Vol 18 (2) ◽  
pp. 346-348 ◽  
Author(s):  
Kamlesh Gidwani ◽  
Albert Picado ◽  
Bart Ostyn ◽  
Shri Prakash Singh ◽  
Rajiv Kumar ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Immunosorbent Assay ◽  
Leishmania Donovani ◽  
Extended Period ◽  
Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Direct Agglutination Test ◽  
History Of ◽  
Antibody Titers

ABSTRACTThe persistence of anti-Leishmania donovaniantibodies in past visceral leishmaniasis (VL) cases was retrospectively assessed by means of the direct agglutination test (DAT) and the rK39 enzyme-linked immunosorbent assay (ELISA). Antibody titers remained high for an extended period of time in past cases of VL. These results highlight the need to carefully elicit the history of patients with VL symptoms.

scholarly journals Hematological Reference Ranges for Apparently Healthy Blood Donors in Debre Markos, North West Ethiopia, 2016

2019 ◽  
Author(s):  
Mekuriaw Mesfin Birhan ◽  
Tigist Tadele ◽  
Diresbachew Haile ◽  
Dessalegn Demeke ◽  
Biruk Getahun ◽  
...  
Keyword(s):  
Blood Donors ◽  
White Blood Cells ◽  
Absolute Lymphocyte Count ◽  
Reference Ranges ◽  
North West ◽  
North West Ethiopia ◽  
Debre Markos ◽  
Wbc Count ◽  
Rbc Count ◽  
Apparently Healthy

Abstract Objective The aim of this study was to determine hematological reference ranges for apparently healthy blood donors in Debre Markos, North West Ethiopia. Result A total of 250 adults with the median age of 20 (range 18 to 48) years were recruited. The mean ± standard deviation of hematological values were white blood cells (WBC) count 6.34±1.82×103/µl (males) and 6.96±1.89×103/µl (females); absolute neutrophil count 3.57±1.47×103/µl (male) and 3.99±1.38×103/µl (females); absolute lymphocyte count, 2.09±0.64×103/µl (males) and 2.27±0.68×103/µl (females); absolute mid-sized cell count 0.68±0.36× 103/µl (males) and 0.70±0.37×103/µl (females); red blood cell (RBC) count 5.13±0.49×106/µL (males) and 4.57±0.36×106/µL (females) platelets, 255.86±65.96 × 103/µl (male) and 280.67±65.71× 103/µl (female).

scholarly journals Seroprevalence of Brucella canis in dogs and at-risk humans in Jordan

2019 ◽  
Vol 64 (No. 6) ◽  
pp. 260-265 ◽  
Author(s):  
M Alshehabat ◽  
M Obaidat ◽  
W Hayajneh
Keyword(s):  
Risk Factors ◽  
At Risk ◽  
Preventive Measures ◽  
Agglutination Test ◽  
Stray Dogs ◽  
Brucella Canis ◽  
Slide Agglutination Test ◽  
Healthy Dogs ◽  
Sera Samples ◽  
Apparently Healthy

Brucella canis infection is a neglected zoonotic disease and its seroprevalence in dogs and at-risk humans has not been previously studied in several countries including Jordan. The main aim of this study was to determine the seroprevalence and identify risk factors of B. canis infection in police, breeding and stray dogs and in at-risk humans in Jordan. A total of 169 sera samples from apparently healthy dogs and 185 samples from apparently healthy people (85 from dog handlers and 100 from the general population) were tested in the study. Antibodies against B. canis were tested using the canine D-Tec<sup>®</sup> CB Rapid Slide Agglutination Test (RSAT) kit with secondary 2-mercaptoethanol (ME-RSAT). Overall, 8.3% of the dog sera samples tested positive to antibodies against B. canis, and 37.8% of stray dogs tested positive. Seroprevalence was higher in male dogs than in females. Furthermore, none of the tested human samples was positive to antibodies against B. canis. There was a significant association between seropositivity and the type of dog. The study reports preliminary findings that suggest the presence of B. canis among stray dogs in Jordan. Thus, preventive measures should be taken to control the transmission of this pathogen from stray dogs to other dogs and humans as well.

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