scholarly journals Loop‐mediated isothermal amplification: Development, validation and application of simple and rapid assays for quantitative detection of species of Arcobacteraceae family‐ and species‐specific Aliarcobacter faecis and Aliarcobacter lanthieri

2020 ◽  
Author(s):  
I.U.H. Khan ◽  
A. Becker ◽  
M. Cloutier ◽  
M. Plötz ◽  
D.R. Lapen ◽  
...  
Keyword(s):  
Isothermal Amplification ◽  
Quantitative Detection ◽  
Loop Mediated Isothermal Amplification ◽  
Rapid Assays ◽  
Species Specific

scholarly journals Novel Multiplex and Loop-Mediated Isothermal Amplification (LAMP) Assays for Rapid Species and Mating-Type Identification of Oculimacula acuformis and O. yallundae (Causal Agents of Cereal Eyespot), and Application for Detection of Ascospore Dispersal and in planta Use

2020 ◽  
Author(s):  
Kevin M. King ◽  
Gavin J. Eyres ◽  
Jon West ◽  
Clara Siraf ◽  
Pavel Matusinsky ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Mating Type ◽  
Field Experiments ◽  
Lamp Assay ◽  
Isothermal Amplification ◽  
In Planta ◽  
Loop Mediated Isothermal Amplification ◽  
Multiplex Pcr Assay ◽  
Species Specific ◽  
Oculimacula Acuformis

Eyespot, caused by the related fungal pathogens Oculimacula acuformis (OA) and O. yallundae (OY), is an important cereal stem-base disease in temperate parts of the world. Both species are dispersed mainly by splash-dispersed conidia but are also known to undergo sexual reproduction yielding apothecia containing ascospores. Field diagnosis of eyespot can be challenging with other pathogens causing similar symptoms, which complicates eyespot management strategies. Differences between OA and OY (e.g. host pathogenicity and fungicide sensitivity) require that both be targeted for effective disease management. Here, we develop and apply two molecular methods for species-specific and mating-type (MAT1-1 or MAT1-2) discrimination of OA and OY isolates. First, a multiplex PCR-based diagnostic assay targeting the MAT idiomorph region was developed allowing simultaneous determination of both species and mating type. This multiplex-PCR assay was successfully applied to type a global collection of isolates. Second, the development of loop-mediated isothermal amplification (LAMP) assays targeting beta-tubulin sequences is described, which allow fast (<9 min) species-specific discrimination of global OA and OY isolates. The LAMP assay can detect very small amounts of target DNA (1 pg) and was successfully applied in planta. In addition, mating-type specific LAMP assays were also developed for rapid (<12 min) genotyping of OA and OY isolates. Finally, the multiplex PCR-based diagnostic was applied, in conjunction with spore trapping in field experiments, to provide evidence of the wind dispersal of ascospores from a diseased crop. The results indicate an important role of the sexual cycle in the dispersal of eyespot.

Colorimetric loop-mediated isothermal amplification (LAMP) for cost-effective and quantitative detection of SARS-CoV-2: the change in color in LAMP-based assays quantitatively correlates with viral copy number

2021 ◽  
Author(s):  
Everardo González-González ◽  
Itzel Montserrat Lara-Mayorga ◽  
Iram Pablo Rodríguez-Sánchez ◽  
Yu Shrike Zhang ◽  
Sergio O. Martínez-Chapa ◽  
...  
Keyword(s):  
Copy Number ◽  
Quantitative Method ◽  
Diagnostic Performance ◽  
Cost Effective ◽  
Isothermal Amplification ◽  
Quantitative Detection ◽  
Loop Mediated Isothermal Amplification ◽  
Viral Copy Number ◽  
Viral Copy

Colorimetric LAMP for COVID-19 intensified diagnostics: a simple and quantitative method comparable in diagnostic performance to RT-qPCR.

scholarly journals Development of a real-time fluorescence loop-mediated isothermal amplification assay for rapid and quantitative detection of Ustilago maydis

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Yanyong Cao ◽  
Lifeng Wang ◽  
Liping Duan ◽  
Jingjing Li ◽  
Juan Ma ◽  
...  
Keyword(s):  
Real Time ◽  
Ustilago Maydis ◽  
Isothermal Amplification ◽  
Quantitative Detection ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Assay

scholarly journals Development of a real-time fluorescence loop-mediated isothermal amplification assay for rapid and quantitative detection ofFusarium oxysporumf. sp.niveumin soil

2013 ◽  
Vol 349 (2) ◽  
pp. 127-134 ◽  
Author(s):  
Jun Peng ◽  
Yuanfeng Zhan ◽  
Fanyun Zeng ◽  
Haibo Long ◽  
Yuelin Pei ◽  
...  
Keyword(s):  
Real Time ◽  
Isothermal Amplification ◽  
Quantitative Detection ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Assay

Technique for Quantitative Detection of Specific DNA Sequences Using Alternately Binding Quenching Probe Competitive Assay Combined with Loop-Mediated Isothermal Amplification

2007 ◽  
Vol 79 (15) ◽  
pp. 5608-5613 ◽  
Author(s):  
Hidenori Tani ◽  
Tatsuya Teramura ◽  
Ken Adachi ◽  
Satoshi Tsuneda ◽  
Shinya Kurata ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Isothermal Amplification ◽  
Quantitative Detection ◽  
Competitive Assay ◽  
Loop Mediated Isothermal Amplification ◽  
Quenching Probe

scholarly journals Real-Time Loop-Mediated Isothermal Amplification (RealAmp) for the Species-Specific Identification of Plasmodium vivax

PLoS ONE ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. e54986 ◽  
Author(s):  
Jaymin C. Patel ◽  
Jenna Oberstaller ◽  
Maniphet Xayavong ◽  
Jothikumar Narayanan ◽  
Jeremy D. DeBarry ◽  
...  
Keyword(s):  
Plasmodium Vivax ◽  
Real Time ◽  
Isothermal Amplification ◽  
Specific Identification ◽  
Loop Mediated Isothermal Amplification ◽  
Species Specific ◽  
Time Loop
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