Production and identification of peptides with activity promoting osteoblast proliferation from meat dregs of Pinctada martensii

2021 ◽  
Author(s):  
Yufeng Tian ◽  
Pingyingzi Jiang ◽  
Xiaoyue Liu ◽  
Lulu Wei ◽  
Yunxia Bai ◽  
...  
Keyword(s):  
Osteoblast Proliferation ◽  
Pinctada Martensii

Isolation and identification of peptides from Pinctada martensii with osteogenic activity

2021 ◽  
Keyword(s):  
Mass Spectrometry ◽  
Alkaline Phosphatase ◽  
Liquid Chromatography Mass Spectrometry ◽  
Osteoblast Proliferation ◽  
Isolation And Identification ◽  
Osteogenic Activity ◽  
Alp Activity ◽  
Pinctada Martensii ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation

Abstract Marine organisms have attracted considerable attention in recent years. In this study, peptides with osteogenic activity from Pinctada martensii were isolated and identified. Additionally, the effects of the hydrolysates on MC3T3-E1 cell proliferation and differentiation were evaluated using the MTT and alkaline phosphatase (ALP) assays, respectively. First, trypsin, pancreatin, and neutral protease were used to hydrolyse the intact shellfish. The hydrolysates with the greatest effects on osteoblast proliferation and ALP activity were separated and purified. Second, fraction WP2 was isolated and purified using a Sephadex G-25 column. WP2, which had the highest osteogenic activity, increased cell growth by 48.57 ± 0.05% and ALP activity by 6.27 ± 0.07 mU. Finally, four novel peptides were identified in WP2 (FDNEGKGKLPEEY, IVLDSGDGVTH, IVLDSGDGVSH, and SSENSDLQRQ) by Orbitrap Fusion Lumos Tribrid orbital liquid chromatography-mass spectrometry. Our findings revealed that P. martensii contains peptides with potential osteogenic activity.

Enhanced osteoblast proliferation and corrosion resistance of c p-Ti through surface nanostructuring by ultrasonic shot peening and stress relieving

2012 ◽  
pp. 121030063702003 ◽  
Author(s):  
Shitu Jindal ◽  
Rajesh Bansal ◽  
B. P. Singh ◽  
Rajiv Pandey ◽  
T. S. N. Shankar Narayanan ◽  
...  
Keyword(s):  
Corrosion Resistance ◽  
Shot Peening ◽  
Osteoblast Proliferation ◽  
Surface Nanostructuring ◽  
Stress Relieving ◽  
Ultrasonic Shot Peening

CLONING AND EXPRESSION CHARACTERIZATION OF DMRT5 IN PINCTADA MARTENSII

2009 ◽  
Vol 33 (5) ◽  
pp. 844-850 ◽  
Author(s):  
Fei-Fei YU ◽  
Jian-Fang GUI ◽  
Li ZHOU ◽  
Mei-Fang WANG ◽  
Xiang-Yong YU
Keyword(s):  
Cloning And Expression ◽  
Pinctada Martensii ◽  
Expression Characterization

Effects of Colostrum Basic Protein from Colostrum Whey Protein: Increases in Osteoblast Proliferation and Bone Metabolism

2007 ◽  
Vol 12 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Jeong-Rai Lee ◽  
Hyun-Mi Kim ◽  
Hee-Sun Choi ◽  
Jeong-Hwa Hong
Keyword(s):  
Bone Metabolism ◽  
Whey Protein ◽  
Basic Protein ◽  
Osteoblast Proliferation

scholarly journals Thyroid-stimulating hormone decreases the risk of osteoporosis by regulating osteoblast proliferation and differentiation

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Tuo Deng ◽  
Wenwen Zhang ◽  
Yanling Zhang ◽  
Mengqi Zhang ◽  
Zhikun Huan ◽  
...  
Keyword(s):  
Thyroid Function ◽  
Thyroid Stimulating Hormone ◽  
Mrna Levels ◽  
Dependent Manner ◽  
Osteoblast Proliferation ◽  
Normal Thyroid ◽  
Normal Thyroid Function ◽  
Gene And Protein Expression ◽  
Proliferation And Differentiation ◽  
Stimulating Hormone

Abstract Background As the incidence of secretory osteoporosis has increased, bone loss, osteoporosis and their relationships with thyroid-stimulating hormone (TSH) have received increased attention. In this study, the role of TSH in bone metabolism and its possible underlying mechanisms were investigated. Methods We analyzed the serum levels of free triiodothyronine (FT3), free thyroxine (FT4), and TSH and the bone mineral density (BMD) levels of 114 men with normal thyroid function. In addition, osteoblasts from rat calvarial samples were treated with different doses of TSH for different lengths of time. The related gene and protein expression levels were investigated. Results A comparison of the BMD between the high-level and low-level serum TSH groups showed that the TSH serum concentration was positively correlated with BMD. TSH at concentrations of 10 mU/mL and 100 mU/mL significantly increased the mRNA levels of ALP, COI1 and Runx2 compared with those of the control (P < 0.05, P < 0.01). Bone morphogenetic protein (BMP)2 activity was enhanced with both increased TSH concentration and increased time. The protein levels of Runx2 and osterix were increased in a dose-dependent manner. Conclusions The circulating concentrations of TSH and BMD were positively correlated with normal thyroid function in males. TSH promoted osteoblast proliferation and differentiation in rat primary osteoblasts.

Nitric Oxide Mediates 17β-Estradiol-Stimulated Human and Rodent Osteoblast Proliferation and Differentiation

2000 ◽  
Vol 277 (3) ◽  
pp. 604-610 ◽  
Author(s):  
Meg C. O'Shaughnessy ◽  
Julia M. Polak ◽  
Faiza Afzal ◽  
Mika V.J. Hukkanen ◽  
Paul Huang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Osteoblast Proliferation ◽  
Proliferation And Differentiation ◽  
17Β Estradiol

scholarly journals In vitro study of human osteoblast proliferation and morphology on orthodontic mini-implants

2015 ◽  
Vol 85 (6) ◽  
pp. 920-926 ◽  
Author(s):  
Ricardo Carvalho Bueno ◽  
Roberta Tarkany Basting
Keyword(s):  
Chemical Composition ◽  
In Vitro Study ◽  
Chemical Components ◽  
Control Group ◽  
Osteoblast Proliferation ◽  
Mini Implants ◽  
Significant Difference ◽  
Implant Anchorage ◽  
Positive Effect

ABSTRACT Objective:  To evaluate the proliferation and morphology of human osteoblasts cultured on two brands of mini-implants after 24, 48, and 72 hours, in addition to the chemical composition found on their surface. Materials and Methods:  Two brands of mini-implant (Morelli and Neodent) were evaluated; polystyrene was used as a control group (n  =  3). Osteoblasts were cultured on the surface of sterilized mini-implants in a CO2 incubator at different time periods (24, 48, and 72 hours). Osteoblast proliferation was quantified by scanning electron microscopy using up to 5000× magnification, and cell morphology was analyzed by a single observer. For the chemical analysis, spectroscopy X-ray fluorescence was used to identify and quantify chemical components on the surface of the mini-implants. Results:  Two-way ANOVA showed no significant interaction between the factors studied (P  =  0.686). A Tukey test revealed no significant difference in osteoblast proliferation between the mini-implants at all studied periods; however, a difference in cell proliferation was detected between the Neodent and the control group (P  =  .025). For all groups, time had a direct and positive effect on osteoblast proliferation (P &lt; .001). The significant elements present in both brands of mini-implants were titanium, aluminum, vanadium, and iron. Conclusions:  Osteoblast proliferation was present on the mini-implants studied, which increased over time; however, no significant difference between brands was observed. No difference was seen between the mini-implants evaluated in terms of chemical composition. Cell adhesion after 72 hours suggests that areas of bone remodeling can be achieved, thus initiating the process of mini-implant anchorage.

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