Effects of Colostrum Basic Protein from Colostrum Whey Protein: Increases in Osteoblast Proliferation and Bone Metabolism

The beneficial effect of bovine whey protein (WP) on bone metabolism has been shown in adult human subjects and ovariectomised rats. However, its effect on bone formation in earlier life, particularly during periods of bone mineral accrual, has not been investigated. Twenty-one male rats (4 weeks old, Wistar strain) were randomised by weight into three groups of seven rats each and fedad libitumon a semi-purified low-Ca diet (3·0 g Ca/kg diet) containing 0 (control), 10 (diet WP1) or 20 (diet WP2) g bovine WP/kg for 47 d. On day 34 of the dietary intervention, all rats had two gelatine capsules containing demineralised bone powder implanted subcutaneously in the thorax region (a well-establishedin vivomodel of ectopic bone formation). At 14 d after implantation, alkaline phosphatase activity (reflective of bone formation) in the bone implants from animals fed WP1 and -2 diets was almost 2-fold (P<0·01) that of control animals. Insulin-like growth factor (IGF)-I mRNA levels were about 3-fold (P<0·05) higher in implants from animals fed the WP diets compared with those from control animals. Serum- and urine-based biomarkers of bone metabolism and bone mineral composition in intact femora were unaffected by WP supplementation. In conclusion, the present findings suggest that bovine WP can enhance the rate of ectopic bone formation in young growing rats fed a Ca-restricted diet. This effect may be mediated by an increased synthesis of IGF-I in growing bone. The effect of WP on bone formation warrants further investigation.

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Milk basic protein increases bone mineral density and improves bone metabolism in healthy young women

Osteoporosis International ◽

10.1007/s00198-006-0228-5 ◽

2006 ◽

Vol 18 (3) ◽

pp. 385-390 ◽

Cited By ~ 55

Author(s):

K. Uenishi ◽

H. Ishida ◽

Y. Toba ◽

S. Aoe ◽

A. Itabashi ◽

...

Keyword(s):

Bone Mineral Density ◽

Bone Metabolism ◽

Bone Mineral ◽

Young Women ◽

Basic Protein ◽

Mineral Density ◽

Milk Basic Protein

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Evaluation of milk basic protein supplementation on bone density and bone metabolism in Chinese young women

European Journal of Nutrition ◽

10.1007/s00394-009-0014-1 ◽

2009 ◽

Vol 48 (5) ◽

pp. 301-306 ◽

Cited By ~ 16

Author(s):

Zhi-Yong Zou ◽

Xiao-Ming Lin ◽

Xian-Rong Xu ◽

Rui Xu ◽

Le Ma ◽

...

Keyword(s):

Bone Density ◽

Bone Metabolism ◽

Young Women ◽

Basic Protein ◽

Protein Supplementation ◽

Milk Basic Protein

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Controlled Trial of the Effects of Milk Basic Protein (MBP) Supplementation on Bone Metabolism in Healthy Adult Women

Bioscience Biotechnology and Biochemistry ◽

10.1271/bbb.65.913 ◽

2001 ◽

Vol 65 (4) ◽

pp. 913-918 ◽

Cited By ~ 69

Author(s):

Seichiro AOE ◽

Yasuhiro TOBA ◽

Jun-ichi YAMAMURA ◽

Hiroshi KAWAKAMI ◽

Masatoshi YAHIRO ◽

...

Keyword(s):

Bone Metabolism ◽

Basic Protein ◽

Healthy Adult ◽

Controlled Trial ◽

Adult Women ◽

Milk Basic Protein

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A controlled trial of the effect of milk basic protein (MBP) supplementation on bone metabolism in healthy menopausal women

Osteoporosis International ◽

10.1007/s00198-005-2012-3 ◽

2005 ◽

Vol 16 (12) ◽

pp. 2123-2128 ◽

Cited By ~ 58

Author(s):

Seiichiro Aoe ◽

Takao Koyama ◽

Yasuhiro Toba ◽

Akira Itabashi ◽

Yukihiro Takada

Keyword(s):

Bone Metabolism ◽

Basic Protein ◽

Controlled Trial ◽

Menopausal Women ◽

Milk Basic Protein

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Markers of Bone Health and Impact of Whey Protein Supplementation in Army Initial Entry Training Soldiers: A Double-Blind Placebo-Controlled Study

Nutrients ◽

10.3390/nu12082225 ◽

2020 ◽

Vol 12 (8) ◽

pp. 2225

Author(s):

JoEllen M. Sefton ◽

Kaitlin D. Lyons ◽

Darren T. Beck ◽

Cody T. Haun ◽

Matthew A. Romero ◽

...

Keyword(s):

Vitamin D ◽

Bone Resorption ◽

Bone Formation ◽

Bone Metabolism ◽

Whey Protein ◽

Fat Mass ◽

Bone Health ◽

Double Blind ◽

Stress Injuries ◽

Initial Entry

Training civilians to be soldiers is a challenging task often resulting in musculoskeletal injuries, especially bone stress injuries. This study evaluated bone health biomarkers (P1NP/CTX) and whey protein or carbohydrate supplementations before and after Army initial entry training (IET). Ninety male IET soldiers participated in this placebo-controlled, double-blind study assessing carbohydrate and whey protein supplementations. Age and fat mass predicted bone formation when controlling for ethnicity, explaining 44% (p < 0.01) of bone formation variations. Age was the only significant predictor of bone resorption (p = 0.02) when controlling for run, fat, and ethnicity, and these factors together explained 32% of the variance in bone resorption during week one (p < 0.01). Vitamin D increased across training (p < 0.01). There was no group by time interaction for supplementation and bone formation (p = 0.75), resorption (p = 0.73), Vitamin D (p = 0.36), or calcium (p = 0.64), indicating no influence of a supplementation on bone biomarkers across training. Age, fitness, fat mass, and ethnicity were important predictors of bone metabolism. The bone resorption/formation ratio suggests IET soldiers are at risk of stress injuries. Male IET soldiers are mildly to moderately deficient in vitamin D and slightly deficient in calcium throughout training. Whey protein or carbohydrate supplementations did not affect the markers of bone metabolism.

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