Production of succinic acid from sugarcane molasses supplemented with a mixture of corn steep liquor powder and peanut meal as nitrogen sources byActinobacillus succinogenes

This review examines the production of the microbial polysaccharide gellan, synthesized by Sphingomonas elodea, on dairy and plant-based processing coproducts. Gellan is a water-soluble gum that structurally exists as a tetrasaccharide comprised of 20% glucuronic acid, 60% glucose and 20% rhamnose, for which various food, non-food and biomedical applications have been reported. A number of carbon and nitrogen sources have been tested to determine whether they can support bacterial gellan production, with several studies attempting to optimize gellan production by varying the culture conditions. The genetics of the biosynthesis of gellan has been explored in a number of investigations and specific genes have been identified that encode the enzymes responsible for the synthesis of this polysaccharide. Genetic mutants exhibiting overproduction of gellan have also been identified and characterized. Several dairy and plant-based processing coproducts have been screened to learn whether they can support the production of gellan in an attempt to lower the cost of synthesizing the microbial polysaccharide. Of the processing coproducts explored, soluble starch as a carbon source supported the highest gellan production by S. elodea grown at 30 °C. The corn processing coproducts corn steep liquor or condensed distillers solubles appear to be effective nitrogen sources for gellan production. It was concluded that further research on producing gellan using a combination of processing coproducts could be an effective solution in lowering its overall production costs.

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Aerobic degradation 2,4-dinitrotoluene: effect of raw organic wastes and nitrogen amendment

10.21203/rs.2.16588/v1 ◽

2019 ◽

Author(s):

Osekhokhune E Okozide ◽

Sunday Adekunle Adebusoye ◽

Oluwafemi Sunday Obayori

Keyword(s):

Corn Steep Liquor ◽

Nitrogen Sources ◽

Tween 80 ◽

16S Rrna Gene Sequencing ◽

Organic Wastes ◽

Rrna Gene ◽

Nitrogen Amendment ◽

Rrna Gene Sequencing ◽

Steep Liquor ◽

The Cost

Abstract 2,4-Dinitrotoluene (2,4-DNT), a major by-product of the synthesis of 2,4,6- trinitrotoluene, is widely used as a waterproofing, plasticizing and gelatinizing agent in propellants and explosives. Due to its toxicity, the compound is treated as a priority pollutant. Therefore, its removal from contaminated systems is a major focus of research and attention. Contaminated sites in Ibadan, Nigeria were screened for the presence of 2,4,-DNT degrading organisms. The technique of continual enrichment on NACs yielded bacterial isolates able to utilize 2,4-DNT as growth substrate. Based on phenotypic characteristics and 16S rRNA gene sequencing one of the isolates selected for further study was identified as Proteus sp. strain OSES2. Growth of the strain on 2,4-DNT resulted in exponential increase in biomass and complete substrate utilization within 72 h accompanied with NO 3 - elimination. Degradation competence enhanced in the presence of Corn steep liquor, molasses and Tween 80 compared to incubation without amendment. Conversely, amendment with nitrogen sources yielded no significant improvement in degradation. Use of this organism organic wastes as candidates in bioremediation strategy should be exploited. This would provide a cheaper organic source supplement for cleanup purposes with the ultimate aim of reducing the cost of bioremediation while reducing wastes intended for landfill.

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Biotechnological Strategies for Chitosan Production by Mucoralean Strains and Dimorphism Using Renewable Substrates

International Journal of Molecular Sciences ◽

10.3390/ijms21124286 ◽

2020 ◽

Vol 21 (12) ◽

pp. 4286

Author(s):

Adriana Ferreira de Souza ◽

Hugo Marques Galindo ◽

Marcos Antônio Barbosa de Lima ◽

Daylin Rubio Ribeaux ◽

Dayana Montero Rodríguez ◽

...

Keyword(s):

Corn Steep Liquor ◽

Nitrogen Sources ◽

Central Point ◽

Carbon And Nitrogen Sources ◽

Carbon And Nitrogen ◽

Deacetylation Degree ◽

Biomass Yields ◽

Steep Liquor ◽

Central Composite ◽

Cassava Waste

We investigated the influence of corn steep liquor (CSL) and cassava waste water (CWW) as carbon and nitrogen sources on the morphology and production of biomass and chitosan by Mucor subtilissimus UCP 1262 and Lichtheimia hyalospora UCP 1266. The highest biomass yields of 4.832 g/L (M. subtilissimus UCP 1262) and 6.345 g/L (L. hyalospora UCP 1266) were produced in assay 2 (6% CSL and 4% CWW), factorial design 22, and also favored higher chitosan production (32.471 mg/g) for M. subtilissimus. The highest chitosan production (44.91 mg/g) by L. hyalospora (UCP 1266) was obtained at the central point (4% of CWW and 6% of CSL). The statistical analysis, the higher concentration of CSL, and lower concentration of CWW significantly contributed to the growth of the strains. The FTIR bands confirmed the deacetylation degree of 80.29% and 83.61% of the chitosan produced by M. subtilissimus (UCP 1262) and L. hyalospora (UCP 1266), respectively. M. subtilissimus (UCP 1262) showed dimorphism in assay 4–6% CSL and 8% CWW and central point. L. hyalospora (UCP 1266) was optimized using a central composite rotational design, and the highest yield of chitosan (63.18 mg/g) was obtained in medium containing 8.82% CSL and 7% CWW. The experimental data suggest that the use of CSL and CWW is a promising association to chitosan production.

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Batch and continuous cultures of Mannheimia succiniciproducens MBEL55E for the production of succinic acid from whey and corn steep liquor

Bioprocess and Biosystems Engineering ◽

10.1007/s00449-003-0341-1 ◽

2003 ◽

Vol 26 (1) ◽

pp. 63-67 ◽

Cited By ~ 74

Author(s):

P. C. Lee ◽

S. Y. Lee ◽

S. H. Hong ◽

H. N. Chang

Keyword(s):

Succinic Acid ◽

Corn Steep Liquor ◽

Continuous Cultures ◽

Mannheimia Succiniciproducens ◽

Steep Liquor

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Optimization and kinetics studies on exopolysaccharide production using Klebsiella oxytoca ICCF 419 strain, and glucose as substrate

ROMANIAN BIOTECHNOLOGICAL LETTERS ◽

10.25083/rbl/26.1/2214.2222 ◽

2021 ◽

Vol 26 (1) ◽

pp. 2214-2222

Author(s):

CATERINA TOMULESCU ◽

MIȘU MOSCOVICI ◽

ROXANA MĂDĂLINA STOICA ◽

GABRIEL BUJOR ALBU ◽

CLAUDIA SEVCENCO ◽

...

Keyword(s):

Corn Steep Liquor ◽

Klebsiella Oxytoca ◽

Nitrogen Sources ◽

Carbon And Nitrogen Sources ◽

Carbon And Nitrogen ◽

Logistic Equations ◽

Maximum Cell ◽

Steep Liquor ◽

Central Composite ◽

Optimal Formula

The present work aimed to investigate the efficiency of kinetic modeling and Response Surface Methodology (RSM), by employing a Central Composite Rotatable Design (CCRD) and to evaluate the effects of carbon and nitrogen sources, inoculation volume, and bioprocess time on an exopolysaccharide (EPS) producer, Klebsiella oxytoca ICCF 419, in batch fermentation with shaken flasks. Among the kinetic models tested, logistic equations were found to fit accordingly, with a correlation coefficient (R2 ) of 0.999. The interaction between glucose and corn steep liquor was found to have a significant impact on the EPS production, and glucose (2% w/v), and a 5% v/v inoculum of 24 hours age, are sufficiently to positively affect the bioprocess, if it is desired only a maximum cell growth, but not associated with the polymer synthesis. The optimal formula predicted by the RSM design was experimentally validated by the following results: 12.16 g EPS/L and 1.65 dry biomass/L, after 48 hours fermentation.

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New Recombinant Escherichia coli Strain Tailored for the Production of Poly(3-Hydroxybutyrate) from Agroindustrial By-Products

Applied and Environmental Microbiology ◽

10.1128/aem.00044-06 ◽

2006 ◽

Vol 72 (6) ◽

pp. 3949-3954 ◽

Cited By ~ 57

Author(s):

Pablo I. Nikel ◽

Alejandra de Almeida ◽

Evelia C. Melillo ◽

Miguel A. Galvagno ◽

M. Julia Pettinari

Keyword(s):

Corn Steep Liquor ◽

Constitutive Expression ◽

Nitrogen Sources ◽

Dry Weight ◽

Coli Strain ◽

Physical Analysis ◽

Lactose Repressor ◽

Expression Of Genes ◽

Steep Liquor ◽

By Products

ABSTRACT A recombinant E. coli strain (K24K) was constructed and evaluated for poly(3-hydroxybutyrate) (PHB) production from whey and corn steep liquor as main carbon and nitrogen sources. This strain bears the pha biosynthetic genes from Azotobacter sp. strain FA8 expressed from a T5 promoter under the control of the lactose operator. K24K does not produce the lactose repressor, ensuring constitutive expression of genes involved in lactose transport and utilization. PHB was efficiently produced by the recombinant strain grown aerobically in fed-batch cultures in a laboratory scale bioreactor on a semisynthetic medium supplemented with the agroindustrial by-products. After 24 h, cells accumulated PHB to 72.9% of their cell dry weight, reaching a volumetric productivity of 2.13 g PHB per liter per hour. Physical analysis of PHB recovered from the recombinants showed that its molecular weight was similar to that of PHB produced by Azotobacter sp. strain FA8 and higher than that of the polymer from Cupriavidus necator and that its glass transition temperature was approximately 20ï¿½C higher than those of PHBs from the natural producer strains.

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