scholarly journals Predicting plant immunity gene expression by identifying the decoding mechanism of calcium signatures

2017 ◽  
Vol 217 (4) ◽  
pp. 1598-1609 ◽  
Author(s):  
Gioia Lenzoni ◽  
Junli Liu ◽  
Marc R. Knight
Keyword(s):  
Gene Expression ◽  
Plant Immunity ◽  
Immunity Gene

scholarly journals HOS15 is a transcriptional corepressor of NPR1-mediated gene activation of plant immunity

2020 ◽  
Vol 117 (48) ◽  
pp. 30805-30815
Author(s):  
Mingzhe Shen ◽  
Chae Jin Lim ◽  
Junghoon Park ◽  
Jeong Eun Kim ◽  
Dongwon Baek ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ubiquitin Ligase ◽  
Transcriptional Activation ◽  
Plant Immunity ◽  
Gene Activation ◽  
Transcriptional Corepressor ◽  
Defense Gene Expression ◽  
Dynamic Regulation ◽  
Defense Gene ◽  
Transcriptional Corepressors

Transcriptional regulation is a complex and pivotal process in living cells. HOS15 is a transcriptional corepressor. Although transcriptional repressors generally have been associated with inactive genes, increasing evidence indicates that, through poorly understood mechanisms, transcriptional corepressors also associate with actively transcribed genes. Here, we show that HOS15 is the substrate receptor for an SCF/CUL1 E3 ubiquitin ligase complex (SCFHOS15) that negatively regulates plant immunity by destabilizing transcriptional activation complexes containing NPR1 and associated transcriptional activators. In unchallenged conditions, HOS15 continuously eliminates NPR1 to prevent inappropriate defense gene expression. Upon defense activation, HOS15 preferentially associates with phosphorylated NPR1 to stimulate rapid degradation of transcriptionally active NPR1 and thus limit the extent of defense gene expression. Our findings indicate that HOS15-mediated ubiquitination and elimination of NPR1 produce effects contrary to those of CUL3-containing ubiquitin ligase that coactivate defense gene expression. Thus, HOS15 plays a key role in the dynamic regulation of pre- and postactivation host defense.

scholarly journals Using FIBexDB for In-Depth Analysis of Flax Lectin Gene Expression in Response to Fusarium oxysporum Infection

Plants ◽  
2022 ◽  
Vol 11 (2) ◽  
pp. 163
Author(s):  
Natalia Petrova ◽  
Natalia Mokshina
Keyword(s):  
Gene Expression ◽  
Fusarium Oxysporum ◽  
Essential Role ◽  
Protein Biosynthesis ◽  
Plant Immunity ◽  
Gene Clustering ◽  
Root Tips ◽  
Lectin Gene ◽  
Depth Analysis ◽  
Lectin Genes

Plant proteins with lectin domains play an essential role in plant immunity modulation, but among a plurality of lectins recruited by plants, only a few members have been functionally characterized. For the analysis of flax lectin gene expression, we used FIBexDB, which includes an efficient algorithm for flax gene expression analysis combining gene clustering and coexpression network analysis. We analyzed the lectin gene expression in various flax tissues, including root tips infected with Fusarium oxysporum. Two pools of lectin genes were revealed: downregulated and upregulated during the infection. Lectins with suppressed gene expression are associated with protein biosynthesis (Calreticulin family), cell wall biosynthesis (galactose-binding lectin family) and cytoskeleton functioning (Malectin family). Among the upregulated lectin genes were those encoding lectins from the Hevein, Nictaba, and GNA families. The main participants from each group are discussed. A list of lectin genes, the expression of which can determine the resistance of flax, is proposed, for example, the genes encoding amaranthins. We demonstrate that FIBexDB is an efficient tool both for the visualization of data, and for searching for the general patterns of lectin genes that may play an essential role in normal plant development and defense.

scholarly journals The Arabidopsis ORA59 coordinates jasmonic acid- and ethylene-responsive gene expression to regulate plant immunity

2021 ◽  
Author(s):  
Young Nam Yang ◽  
Youngseong Kim ◽  
Hyeri Kim ◽  
Su Jin Kim ◽  
Kwang-Moon Cho ◽  
...  
Keyword(s):  
Gene Expression ◽  
Jasmonic Acid ◽  
Plant Immunity ◽  
Gene Promoters ◽  
Response Factors ◽  
Cis Element ◽  
Transcriptional Reprogramming ◽  
Gcc Box ◽  
Gene Expression Dynamics ◽  
Direct Binding

Jasmonic acid (JA) and ethylene (ET) signaling modulate plant defense against necrotrophic pathogens. These hormone pathways lead to transcriptional reprogramming, which is a major part of plant immunity and requires the roles of transcription factors. ET response factors are responsible for the transcriptional regulation of JA/ET-responsive defense genes, among which ORA59 functions as a key regulator of this process and has been implicated in the JA-ET crosstalk. Here, we identified the ERELEE4 as an ORA59-binding cis-element, in addition to the well-characterized GCC box, demonstrating that ORA59 regulates JA/ET-responsive genes through direct binding to these elements in the gene promoters. Notably, ORA59 exhibited differential preference for the GCC box and ERELEE4, depending on whether ORA59 activation is achieved by JA and ET, respectively. Our results provide insights into how ORA59 can generate specific patterns of gene expression dynamics through JA and ET hormone pathways.

scholarly journals Ulvan, a Sulfated Polysaccharide from Green Algae, Activates Plant Immunity through the Jasmonic Acid Signaling Pathway

2010 ◽  
Vol 2010 ◽  
pp. 1-11 ◽  
Author(s):  
Valérie Jaulneau ◽  
Claude Lafitte ◽  
Christophe Jacquet ◽  
Sylvie Fournier ◽  
Sylvie Salamagne ◽  
...  
Keyword(s):  
Gene Expression ◽  
Jasmonic Acid ◽  
Green Algae ◽  
Plant Immunity ◽  
Gene Promoter ◽  
Gene Expression Signature ◽  
Sulfated Polysaccharide ◽  
Promoter Gene ◽  
Potential Reservoir ◽  
Hormonal Mutants

The industrial use of elicitors as alternative tools for disease control needs the identification of abundant sources of them. We report on an elicitor obtained from the green algaeUlvaspp. A fraction containing most exclusively the sulfated polysaccharide known as ulvan-induced expression of a GUS gene placed under the control of a lipoxygenase gene promoter. Gene expression profiling was performed upon ulvan treatments onMedicago truncatulaand compared to phytohormone effects. Ulvan induced a gene expression signature similar to that observed upon methyl jasmonate treatment (MeJA). Involvement of jasmonic acid (JA) in ulvan response was confirmed by detecting induction of protease inhibitory activity and by hormonal profiling of JA, salicylic acid (SA) and abscisic acid (ABA). Ulvan activity on the hormonal pathway was further consolidated by usingArabidopsishormonal mutants. Altogether, our results demonstrate that green algae are a potential reservoir of ulvan elicitor which acts through the JA pathway.

scholarly journals Prior exposure of Arabidopsis seedlings to mechanical stress heightens jasmonic acid-mediated defense against necrotrophic pathogens

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Eric Brenya ◽  
Zhong-Hua Chen ◽  
David Tissue ◽  
Alexie Papanicolaou ◽  
Christopher Ian Cazzonelli
Keyword(s):  
Gene Expression ◽  
Cell Wall ◽  
Jasmonic Acid ◽  
Mechanical Stress ◽  
Plant Immunity ◽  
Alternaria Brassicicola ◽  
Marker Genes ◽  
Subsequent Challenge ◽  
Short Period ◽  
Wall Appositions

Abstract Background Prolonged mechanical stress (MS) causes thigmomorphogenesis, a stress acclimation response associated with increased disease resistance. What remains unclear is if; 1) plants pre-exposed to a short period of repetitive MS can prime defence responses upon subsequent challenge with necrotrophic pathogens, 2) MS mediates plant immunity via jasmonic acid (JA) signalling, and 3) a short period of repetitive MS can cause long-term changes in gene expression resembling a stress-induced memory. To address these points, 10-days old juvenile Arabidopsis seedlings were mechanically stressed for 7-days using a soft brush and subsequently challenged with the necrotrophic pathogens, Alternaria brassicicola, and Botrytis cinerea. Here we assessed how MS impacted structural cell wall appositions, disease symptoms and altered gene expression in response to infection. Results The MS-treated plants exhibited enhanced cell wall appositions and jasmonic acid (JA) accumulation that correlated with a reduction in disease progression compared to unstressed plants. The expression of genes involved in JA signalling, callose deposition, peroxidase and phytoalexin biosynthesis and reactive oxygen species detoxification were hyper-induced 4-days post-infection in MS-treated plants. The loss-of-function in JA signalling mediated by the JA-insensitive coronatine-insensitive 1 (coi1) mutant impaired the hyper-induction of defense gene expression and promoted pathogen proliferation in MS-treated plants subject to infection. The basal expression level of PATHOGENESIS-RELATED GENE 1 and PLANT DEFENSIN 1.2 defense marker genes were constitutively upregulated in rosette leaves for 5-days post-MS, as well as in naïve cauline leaves that differentiated from the inflorescence meristem well after ceasing MS. Conclusion This study reveals that exposure of juvenile Arabidopsis plants to a short repetitive period of MS can alter gene expression and prime plant resistance upon subsequent challenge with necrotrophic pathogens via the JA-mediated COI1 signalling pathway. MS may facilitate a stress-induced memory to modulate the plant’s response to future stress encounters. These data advance our understanding of how MS primes plant immunity against necrotrophic pathogens and how that could be utilised in sustainable agricultural practices.

scholarly journals DEAF-1 regulates immunity gene expression in Drosophila

2008 ◽  
Vol 105 (24) ◽  
pp. 8351-8356 ◽  
Author(s):  
D. E. Reed ◽  
X. M. Huang ◽  
J. A. Wohlschlegel ◽  
M. S. Levine ◽  
K. Senger
Keyword(s):  
Gene Expression ◽  
Immunity Gene

Write 'systemic small RNAs': read 'systemic immunity'

2011 ◽  
Vol 38 (10) ◽  
pp. 747
Author(s):  
Alireza Seifi
Keyword(s):  
Gene Expression ◽  
Small Rnas ◽  
Rna Silencing ◽  
Mammalian Cells ◽  
Systemic Acquired Resistance ◽  
Plant Immunity ◽  
Acquired Resistance ◽  
Plant Molecular Biology ◽  
Potential Contribution ◽  
Systemic Immunity

About 50 years ago, it was reported that pathogen-infected plants are less susceptible to a broad spectrum of the subsequent pathogen attacks. This form of induced resistance, which resembles the immunisation in mammalian cells, is called systemic acquired resistance (SAR). In the last 10 years, plant molecular biology has been revolutionised by the discovery of RNA silencing, which is also a systemic phenomenon and also contributes to plant immunity. Here, I review these two systemic phenomena in a comparative way to highlight the possibility that systemic silencing contributes to systemic immunity. This potential contribution could be in the process of gene expression reprogramming, which is needed for SAR induction, and/or in SAR signal complex, and/or in establishing SAR in remote tissues and forming priming status.

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