CYP81A P450s are involved in concomitant cross‐resistance to acetolactate synthase and acetyl‐CoA carboxylase herbicides inEchinochloa phyllopogon

Diclofop-resistant Italian ryegrass is widespread in southwestern North Carolina, and growers have resorted to using acetolactate synthase (ALS) inhibitors such as mesosulfuron and pyroxsulam to control this weed in wheat. In the spring of 2007, mesosulfuron failed to control Italian ryegrass in several wheat fields. Seed were collected from six fields in two counties and greenhouse studies were conducted to determine response to mesosulfuron and the acetyl-CoA carboxylase (ACCase) inhibitors diclofop and pinoxaden. All populations were resistant to diclofop and cross-resistant to pinoxaden. Five of the six populations were resistant to diclofop, pinoxaden, and mesosulfuron. An additional study with two biotypes confirmed cross-resistance to the ALS inhibitors imazamox, mesosulfuron, and pyroxsulam. Resistance to mesosulfuron was heritable.

Download Full-text

Cross resistance patterns to acetyl-CoA carboxylase inhibiting herbicides associated with different mutations in Italian ryegrass from China

Crop Protection ◽

10.1016/j.cropro.2020.105479 ◽

2020 ◽

pp. 105479

Author(s):

Pei Zhang ◽

Yangyang Zhang ◽

Xiling Chen ◽

Liyao Dong

Keyword(s):

Italian Ryegrass ◽

Cross Resistance ◽

Acetyl Coa Carboxylase ◽

Acetyl Coa ◽

Resistance Patterns

Download Full-text

Characterization of cross-resistance patterns in acetyl-CoA carboxylase inhibitor resistant wild oat (Avena fatua)

Weed Science ◽

10.1017/s0043174500088925 ◽

1997 ◽

Vol 45 (6) ◽

pp. 750-755 ◽

Cited By ~ 34

Author(s):

Luc Bourgeois ◽

Norm C. Kenkel ◽

Ian N. Morrison

Keyword(s):

Cluster Analysis ◽

Principal Component ◽

Petri Dish ◽

Cross Resistance ◽

Wild Oat ◽

Acetyl Coa Carboxylase ◽

Acetyl Coa ◽

Pairwise Correlation ◽

Resistance Patterns

The purpose of this study was to determine cross-resistance patterns among wild oat lines resistant to acetyl-CoA carboxylase (ACCase) inhibitors and to determine which, if any, cross-resistant type was more common than another. Discriminatory concentrations of two aryloxyphenoxy-propionates (APP) and three cyclohexanediones (CHD) were determined using a petri-dish bioassay. These concentrations were then applied to 82 resistant wild oat lines identified in previous studies. In addition, two resistant standards (UM1 and UM33) and a susceptible standard (UM5) were included in the experiments. Coleoptile lengths expressed as percentages of untreated controls were used to assess the level of resistance to each herbicide. Large variations were observed among wild oat lines and herbicides. However, cluster analysis summarized the relationship between the five herbicides (variables) and the wild oat lines into three main cross-resistance types. Type A included wild oat lines with high resistance to APP herbicides and no or low resistance to CHD herbicides. Types B and C included those with low to moderate resistant and high levels of resistance to all five herbicides, respectively. Type C was the most common cross-resistance type. Relationships among herbicides were determined using pairwise correlation and principal component analysis (PCA). All correlations were high between APP herbicides and between CHD herbicides but not between APP and CHD herbicides. The first two axes of the PCA accounted for 88.4% of the total variance, with the first axis correlated to the CHD herbicides and the second axis correlated to the APP herbicides. In the PCA, wild oat lines were segregated into the three types identified in the cluster analysis. Although CHD and APP herbicides bind at the same region on the ACCase, resistant wild oat lines respond differently to them.

Download Full-text

Cross-resistance to acetyl-CoA carboxylase–inhibiting herbicides conferred by a target-site mutation in perennial ryegrass (Lolium perenne) from Argentina

Weed Science ◽

10.1017/wsc.2020.1 ◽

2020 ◽

Vol 68 (2) ◽

pp. 116-124 ◽

Cited By ~ 2

Author(s):

Marcos Yanniccari ◽

Ramón Gigón

Keyword(s):

Seed Production ◽

Lolium Perenne ◽

Perennial Ryegrass ◽

Cross Resistance ◽

Acetyl Coa Carboxylase ◽

Acetyl Coa ◽

Plant Survival ◽

Winter Cereal ◽

Susceptible Population ◽

Resistant Population

AbstractIn Argentina, Lolium spp. occur in 40% of winter cereal crops from the Pampas. Several years ago, cases of glyphosate-resistant perennial ryegrass (Lolium perenne L.) were detected, and the use of acetyl-CoA carboxylase (ACCase)-inhibiting herbicides to eradicate these plants has been considered. The aim of this study was to evaluate the sensitivity of a putative pinoxaden-resistant L. perenne population to ACCase-inhibiting herbicides. Around 80% of plants from the putative resistant population survived at a recommended dose of pinoxaden, and they produced viable seeds. The resistance indices (RIs) to pinoxaden were 5.1 and 2.8 for plant survival and seed production, respectively. A single point mutation that conferred a Asp-2078-Gly substitution in ACCase was the source of the resistance. To match the plant control achieved in the susceptible population, the resistant population required 5.4- and 10.4-fold greater doses of clethodim and quizalofop, respectively. RIs for viable seed production when treated with clethodim and quizalofop were 3.3 and 6.6, respectively. The Asp-2078-Gly mutation endowed significant levels of resistance to pinoxaden, clethodim, and quizalofop. For three herbicides, the level of resistance of a pinoxaden-resistant L. perenne population to ACCase inhibitors was evaluated, based on an evaluation of dose response for plant survival and seed production. The RIs were higher for plant survival than for seed production. In Argentina, the selection pressure associated with clethodim and haloxifop preplant application and pinoxaden use on wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) crops, would have favored the propagation of the Asp-2078-Gly mutation with its associated resistance.

Download Full-text

First Detection and Characterization of Cross- and Multiple Resistance to Acetyl-CoA Carboxylase (ACCase)- and Acetolactate Synthase (ALS)-Inhibiting Herbicides in Black-Grass (Alopecurus myosuroides) and Italian Ryegrass (Lolium multiflorum) Populations from Ireland

Agriculture ◽

10.3390/agriculture11121272 ◽

2021 ◽

Vol 11 (12) ◽

pp. 1272

Author(s):

Vijaya Bhaskar Alwarnaidu Vijayarajan ◽

Patrick D Forristal ◽

Sarah K Cook ◽

David Schilder ◽

Jimmy Staples ◽

...

Keyword(s):

Lolium Multiflorum ◽

Acetolactate Synthase ◽

Target Site ◽

Italian Ryegrass ◽

Integrated Weed Management ◽

Multiple Resistance ◽

Acetyl Coa Carboxylase ◽

Acetyl Coa ◽

Alopecurus Myosuroides ◽

Target Site Resistance

Understanding the resistance spectrum and underlying genetic mechanisms is critical for managing herbicide-resistant populations. In this study, resistance to acetyl CoA carboxylase (ACCase) and acetolactate synthase (ALS) inhibitors was investigated in four suspected resistant populations of Alopecurus myosuroides (ALOMY-001 to ALOMY-004) and Lolium multiflorum (LOLMU-001 to LOLMU-004), collected from cereal production fields in Ireland. Glasshouse assays with three ALOMY-active herbicides [propaquizafop, cycloxydim (ACCase) and mesosulfuron + iodosulfuron (ALS)] or five LOLMU-active herbicides [pinoxaden, propaquizafop, cycloxydim (ACCase) and mesosulfuron + iodosulfuron, pyroxsulam (ALS)], and target-site resistance mechanism studies, based on pyrosequencing, were carried out in each of those populations. For A. myosuroides, Ile-1781-Leu ACCase mutation contributed to propaquizafop and cycloxydim resistance (shoot dry weight GR50 resistance factor (RF) = 7.5–35.5) in all ALOMY populations, and the independent Pro-197-Thr or Pro-197-Ser ALS mutation contributed to mesosulfuron + iodosulfuron resistance (RF = 3.6–6.6), in ALOMY-002 to ALOMY-004. Most of the analyzed plants for these mutations were homo/heterozygous combinations or only heterozygous. For L. multiflorum, phenotypic resistance to mesosulfuron + iodosulfuron (RF = 11.9–14.6) and pyroxsulam (RF = 2.3–3.1) was noted in all LOLMU populations, but the Pro-197-Gln or Pro-197-Leu ALS mutation (mostly in homozygous status) was identified in LOLMU-001, LOLMU-002 and LOLMU-004 only. Additionally, despite no known ACCase mutations in any LOLMU populations, LOLMU-002 survived pinoxaden and propaquizafop application (RF = 3.4 or 1.3), and LOLMU-003 survived pinoxaden (RF = 2.3), suggesting the possibility of non-target-site resistance mechanisms for ACCase and/or ALS resistance in these populations. Different resistance levels, as evidenced by a reduction in growth as dose increased above field rates in ALOMY and LOLMU, were due to variations in mutation rate and the level of heterozygosity, resulting in an overall resistance rating of low to moderate. This is the first study confirming cross- and multiple resistance to ACCase- and ALS-inhibiting herbicides, highlighting that resistance monitoring in A. myosuroides and L. multiflorum in Ireland is critical, and the adoption of integrated weed management strategies (chemical and non-chemical/cultural strategies) is essential.

Download Full-text

Cross Resistance of Acetyl-coa Carboxylase (ACCase) Inhibitor–Resistant Wild Oat (Avena fatua) Biotypes in the Pacific Northwest

Weed Technology ◽

10.1614/we-07-105.1 ◽

2008 ◽

Vol 22 (1) ◽

pp. 142-145 ◽

Cited By ~ 19

Author(s):

Ahmet Uludag ◽

Kee Woong Park ◽

Joshua Cannon ◽

Carol A. Mallory-Smith

Keyword(s):

Pacific Northwest ◽

Avena Fatua ◽

Cross Resistance ◽

Wild Oat ◽

Acetyl Coa Carboxylase ◽

Acetyl Coa ◽

The Pacific

Download Full-text

Isolation and expression of genes for acetolactate synthase and acetyl-CoA carboxylase in Echinochloa phyllopogon, a polyploid weed species

Pest Management Science ◽

10.1002/ps.3287 ◽

2012 ◽

Vol 68 (7) ◽

pp. 1098-1106 ◽

Cited By ~ 52

Author(s):

Satoshi Iwakami ◽

Akira Uchino ◽

Hiroaki Watanabe ◽

Yuji Yamasue ◽

Tatsuya Inamura

Keyword(s):

Acetolactate Synthase ◽

Acetyl Coa Carboxylase ◽

Weed Species ◽

Acetyl Coa ◽

Expression Of Genes ◽

Echinochloa Phyllopogon

Download Full-text

Mesosulfuron-Resistant Italian Ryegrass (Lolium Multiflorum) Biotype from Texas

Weed Technology ◽

10.1614/wt-08-032.1 ◽

2008 ◽

Vol 22 (3) ◽

pp. 431-434 ◽

Cited By ~ 9

Author(s):

Andrew T. Ellis ◽

Gaylon D. Morgan ◽

Thomas C. Mueller

Keyword(s):

Winter Wheat ◽

Plant Biomass ◽

Lolium Multiflorum ◽

Acetolactate Synthase ◽

Italian Ryegrass ◽

Acetyl Coa Carboxylase ◽

Acetyl Coa ◽

Resistance Level ◽

Greenhouse Experiments

Acetolactate synthase (ALS)–inhibiting herbicides are often used to control Italian ryegrass in winter wheat in Texas. An Italian ryegrass biotype near Waco, TX was evaluated for resistance to mesosulfuron in field and greenhouse experiments. Control of the biotype in the field was less than 10% with the label rate of mesosulfuron (15 g ai/ha). Greenhouse studies confirmed that the biotype was resistant to mesosulfuron; control of the biotype was less than 35% at 120 g ai/ha mesosulfuron. The herbicide dose required to reduce plant biomass of a susceptible and the Waco biotype by 50% (GR50) was 1.3 and 31 g ai/ha, respectively, indicating a resistance level of 24-fold in the Waco biotype. However, the Waco biotype was controlled with the acetyl-CoA carboxylase inhibitors diclofop and pinoxaden.

Download Full-text

Resistance Profile of Diclofop-Resistant Italian Ryegrass (Lolium multiflorum) to ACCase- and ALS-Inhibiting Herbicides in Arkansas, USA

Weed Science ◽

10.1614/ws-08-003.1 ◽

2008 ◽

Vol 56 (4) ◽

pp. 614-623 ◽

Cited By ~ 31

Author(s):

Yong In Kuk ◽

Nilda R. Burgos ◽

Robert C. Scott

Keyword(s):

Lolium Multiflorum ◽

Acetolactate Synthase ◽

Italian Ryegrass ◽

Cross Resistance ◽

Southern United States ◽

Resistance Pattern ◽

Acetyl Coa Carboxylase ◽

Wheat Production ◽

Resistant Accession ◽

Als Inhibitors

Diclofop-resistant Italian ryegrass is a major weed problem in wheat production. This study aimed to determine the resistance pattern of diclofop-resistant Italian ryegrass accessions from the southern United States to the latest commercialized herbicides for wheat production, pinoxaden and mesosulfuron, and to other acetolactate synthase (ALS) and acetyl-CoA carboxylase (ACCase) inhibitors. Twenty-nine of 36 accessions were resistant to the commercial dose of diclofop. The majority (80%) of diclofop-resistant accessions were also resistant to clodinafop. Of 25 diclofop-resistant accessions, 5 were resistant to pinoxaden. All accessions tested were susceptible to the commercial dose of clethodim and sethoxydim. The cross-resistance pattern of diclofop-resistant Italian ryegrass to other ACCase inhibitors was 20% for pinoxaden and none with clethodim or sethoxydim. One accession was resistant to mesosulfuron but not to diclofop. This mesosulfuron-resistant accession was cross-resistant to sulfometuron but not to imazamox. All diclofop-resistant accessions tested were susceptible to ALS inhibitors, mesosulfuron, sulfometuron, and imazamox. Therefore, diclofop-resistant Italian ryegrass in Arkansas can be controlled with imazamox (in Clearfield wheat) and can mostly be controlled with mesosulfuron and pinoxaden. It could also be controlled by other selective grass herbicides in broadleaf crops.

Download Full-text

Biochemical and structural characterization of quizalofop-resistant wheat acetyl-CoA carboxylase

Scientific Reports ◽

10.1038/s41598-021-04280-x ◽

2022 ◽

Vol 12 (1) ◽

Author(s):

Raven Bough ◽

Franck E. Dayan

Keyword(s):

Amino Acid ◽

Winter Wheat ◽

Amino Acid Substitution ◽

Reference Sequence ◽

Cross Resistance ◽

Homozygous Mutation ◽

Acetyl Coa Carboxylase ◽

Acetyl Coa ◽

Nucleotide Mutation

AbstractA novel nucleotide mutation in ACC1 resulting in an alanine to valine amino acid substitution in acetyl-CoA carboxylase (ACCase) at position 2004 of the Alopecurus myosuroides reference sequence (A2004V) imparts quizalofop resistance in wheat. Genotypes endowed with the homozygous mutation in one or two ACC1 homoeologs are seven- and 68-fold more resistant to quizalofop than a wildtype winter wheat in greenhouse experiments, respectively. In vitro ACCase activities in soluble protein extracts from these varieties are 3.8- and 39.4-fold more resistant to quizalofop with the homozygous mutation in either one or two genomes, relative to the wildtype. The A2004V mutation does not alter the specific activity of wheat ACCase, suggesting that this resistance trait does not affect the catalytic functions of ACCase. Modeling of wildtype and quizalofop-resistant wheat ACCase demonstrates that the A2004V amino acid substitution causes a reduction in the volume of the binding pocket that hinders quizalofop’s interaction with ACCase. Docking studies confirm that the mutation reduces the binding affinity of quizalofop. Interestingly, the models suggest that the A2004V mutation does not affect haloxyfop binding. Follow up in vivo and in vitro experiments reveal that the mutation, in fact, imparts negative cross-resistance to haloxyfop, with quizalofop-resistant varieties exhibiting higher sensitivity to haloxyfop than the wildtype winter wheat line.

Download Full-text