Mesosulfuron-Resistant Italian Ryegrass (Lolium Multiflorum) Biotype from Texas

2008 ◽  
Vol 22 (3) ◽  
pp. 431-434 ◽  
Author(s):  
Andrew T. Ellis ◽  
Gaylon D. Morgan ◽  
Thomas C. Mueller
Keyword(s):  
Winter Wheat ◽  
Plant Biomass ◽  
Lolium Multiflorum ◽  
Acetolactate Synthase ◽  
Italian Ryegrass ◽  
Acetyl Coa Carboxylase ◽  
Acetyl Coa ◽  
Resistance Level ◽  
Greenhouse Experiments

Acetolactate synthase (ALS)–inhibiting herbicides are often used to control Italian ryegrass in winter wheat in Texas. An Italian ryegrass biotype near Waco, TX was evaluated for resistance to mesosulfuron in field and greenhouse experiments. Control of the biotype in the field was less than 10% with the label rate of mesosulfuron (15 g ai/ha). Greenhouse studies confirmed that the biotype was resistant to mesosulfuron; control of the biotype was less than 35% at 120 g ai/ha mesosulfuron. The herbicide dose required to reduce plant biomass of a susceptible and the Waco biotype by 50% (GR50) was 1.3 and 31 g ai/ha, respectively, indicating a resistance level of 24-fold in the Waco biotype. However, the Waco biotype was controlled with the acetyl-CoA carboxylase inhibitors diclofop and pinoxaden.

Control of Italian Ryegrass (Lolium multiflorum) in Winter Wheat

2005 ◽  
Vol 19 (2) ◽  
pp. 261-265 ◽  
Author(s):  
Aaron J. Hoskins ◽  
Bryan G. Young ◽  
Ronald F. Krausz ◽  
John S. Russin
Keyword(s):  
Winter Wheat ◽  
Yield Loss ◽  
Lolium Multiflorum ◽  
Wheat Yield ◽  
Acetolactate Synthase ◽  
Field Studies ◽  
Italian Ryegrass ◽  
Acetyl Coa Carboxylase ◽  
Application Timing ◽  
Foliar Symptoms

Field studies were established in 1999 and 2000 to evaluate Italian ryegrass, wheat, and double-crop soybean response to fall and spring postemergence applications of flucarbazone, sulfosulfuron, clodinafop, diclofop, and tralkoxydim applied alone and in combination with thifensulfuron + tribenuron to winter wheat. Fall-applied herbicides caused 5% or less wheat injury. Spring-applied herbicides caused 3 to 45% wheat injury, and the greatest injury occurred with the combination of flucarbazone with thifensulfuron + tribenuron in the spring of 2001. Spring-applied sulfosulfuron, tralkoxydim, diclofop, and clodinafop caused 3 to 6% and 16 to 26% wheat injury in 2000 and 2001, respectively. Herbicide injury to wheat did not reduce wheat grain yield compared with the hand-weeded treatment. Italian ryegrass competition in the nontreated plots reduced wheat yield by as much as 33% compared with herbicide-treated plots. Italian ryegrass control was 89 to 99% from clodinafop and diclofop and 78 to 97% from flucarbazone, with no differences because of application timing in either year of the study. Italian ryegrass control from sulfosulfuron and tralkoxydim was greater from the spring of 2000 applications (94 to 99%) compared with the fall of 1999 applications (65 to 88%). However, in 2001, application timing (fall vs. spring) for sulfosulfuron and tralkoxydim did not affect Italian ryegrass control. Thifensulfuron + tribenuron combined with tralkoxydim reduced control of Italian ryegrass control compared with tralkoxydim alone in both years of the study. Italian ryegrass control was not reduced when thifensulfuron + tribenuron was combined with sulfosulfuron, flucarbazone, diclofop, or clodinafop. Italian ryegrass was controlled effectively by the acetyl-CoA carboxylase–inhibiting herbicides diclofop, clodinafop, and tralkoxydim. However, control of Italian ryegrass with the acetolactate synthase–inhibiting herbicides flucarbazone and sulfosulfuron was inconsistent. Double-crop soybean after wheat did not have foliar symptoms or yield loss from fall- or spring-applied herbicides.

scholarly journals First Detection and Characterization of Cross- and Multiple Resistance to Acetyl-CoA Carboxylase (ACCase)- and Acetolactate Synthase (ALS)-Inhibiting Herbicides in Black-Grass (Alopecurus myosuroides) and Italian Ryegrass (Lolium multiflorum) Populations from Ireland

Agriculture ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1272
Author(s):  
Vijaya Bhaskar Alwarnaidu Vijayarajan ◽  
Patrick D Forristal ◽  
Sarah K Cook ◽  
David Schilder ◽  
Jimmy Staples ◽  
...  
Keyword(s):  
Lolium Multiflorum ◽  
Acetolactate Synthase ◽  
Target Site ◽  
Italian Ryegrass ◽  
Integrated Weed Management ◽  
Multiple Resistance ◽  
Acetyl Coa Carboxylase ◽  
Acetyl Coa ◽  
Alopecurus Myosuroides ◽  
Target Site Resistance

Understanding the resistance spectrum and underlying genetic mechanisms is critical for managing herbicide-resistant populations. In this study, resistance to acetyl CoA carboxylase (ACCase) and acetolactate synthase (ALS) inhibitors was investigated in four suspected resistant populations of Alopecurus myosuroides (ALOMY-001 to ALOMY-004) and Lolium multiflorum (LOLMU-001 to LOLMU-004), collected from cereal production fields in Ireland. Glasshouse assays with three ALOMY-active herbicides [propaquizafop, cycloxydim (ACCase) and mesosulfuron + iodosulfuron (ALS)] or five LOLMU-active herbicides [pinoxaden, propaquizafop, cycloxydim (ACCase) and mesosulfuron + iodosulfuron, pyroxsulam (ALS)], and target-site resistance mechanism studies, based on pyrosequencing, were carried out in each of those populations. For A. myosuroides, Ile-1781-Leu ACCase mutation contributed to propaquizafop and cycloxydim resistance (shoot dry weight GR50 resistance factor (RF) = 7.5–35.5) in all ALOMY populations, and the independent Pro-197-Thr or Pro-197-Ser ALS mutation contributed to mesosulfuron + iodosulfuron resistance (RF = 3.6–6.6), in ALOMY-002 to ALOMY-004. Most of the analyzed plants for these mutations were homo/heterozygous combinations or only heterozygous. For L. multiflorum, phenotypic resistance to mesosulfuron + iodosulfuron (RF = 11.9–14.6) and pyroxsulam (RF = 2.3–3.1) was noted in all LOLMU populations, but the Pro-197-Gln or Pro-197-Leu ALS mutation (mostly in homozygous status) was identified in LOLMU-001, LOLMU-002 and LOLMU-004 only. Additionally, despite no known ACCase mutations in any LOLMU populations, LOLMU-002 survived pinoxaden and propaquizafop application (RF = 3.4 or 1.3), and LOLMU-003 survived pinoxaden (RF = 2.3), suggesting the possibility of non-target-site resistance mechanisms for ACCase and/or ALS resistance in these populations. Different resistance levels, as evidenced by a reduction in growth as dose increased above field rates in ALOMY and LOLMU, were due to variations in mutation rate and the level of heterozygosity, resulting in an overall resistance rating of low to moderate. This is the first study confirming cross- and multiple resistance to ACCase- and ALS-inhibiting herbicides, highlighting that resistance monitoring in A. myosuroides and L. multiflorum in Ireland is critical, and the adoption of integrated weed management strategies (chemical and non-chemical/cultural strategies) is essential.

scholarly journals The Effect of Foliar and Soil Application of Flufenacet and Prosulfocarb on Italian Ryegrass (Lolium multiflorum L.) Control

Agriculture ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 552
Author(s):  
Christian Andreasen ◽  
Kasper Lund Høgh ◽  
Signe Marie Jensen
Keyword(s):  
Foliar Application ◽  
Lolium Multiflorum ◽  
Acetolactate Synthase ◽  
Italian Ryegrass ◽  
High Yield ◽  
Acetyl Coa Carboxylase ◽  
Yield Losses ◽  
Soil Application ◽  
Greenhouse Experiments ◽  
Foliar Biomass

Italian ryegrass (Lolium multiflorum Lam.) can be a troublesome weed that may causes high yield losses to several crops. Ryegrass resistance to the typically used acetolactate synthase (ALS) and acetyl-CoA carboxylase (ACCase) inhibiting herbicides complicates the control. As an alternative, we evaluated and compared the effects of two soil-acting herbicides, flufenacet and prosulfocarb, on susceptible L. multiflorum. The herbicides were applied in two doses in three different methods of applications: (1) soil and foliar application (2) foliar application, and (3) soil application only. Two greenhouse experiments separated in time showed that both herbicides reduced root and foliar biomass significantly as compared to the nontreated plants. In experiment 1, both herbicides resulted in lower efficacy when they only were applied to the leaves compared to the nontreated plants. Especially the foliar effect of flufenacet was small. The highest dose of prosulfocarb (4200 g ai ha−1) reduced the fresh foliar weight by 61% in experiment 1 and by 95% in experiment 2. The lowest dose of prosulfocarb (2100 g ai ha−1) reduced the weights by 73% (experiment 1) and 98% (experiment 2), respectively. For both herbicides the soil and foliar application applied postemergence were effective in reducing growth of L. multiflorum significantly in both experiments. Foliar application showed inconsistent results, showing that soil absorption plays an important role on herbicide efficacy even when the herbicides are applied postemergence. Postemergence application of prosulfocarb and flufenacet were effective to reduce L. multiflorum growth having apparently good root and leaf absorption.

Resistance to Acetolactate Synthase and Acetyl Co-A Carboxylase Inhibitors in North Carolina Italian Ryegrass (Lolium perenne)

2011 ◽  
Vol 25 (4) ◽  
pp. 659-666 ◽  
Author(s):  
Aman Chandi ◽  
Alan C. York ◽  
David L. Jordan ◽  
Josh B. Beam
Keyword(s):  
North Carolina ◽  
Lolium Perenne ◽  
Acetolactate Synthase ◽  
Italian Ryegrass ◽  
Cross Resistance ◽  
Acetyl Coa Carboxylase ◽  
Acetyl Coa ◽  
Als Inhibitors

Diclofop-resistant Italian ryegrass is widespread in southwestern North Carolina, and growers have resorted to using acetolactate synthase (ALS) inhibitors such as mesosulfuron and pyroxsulam to control this weed in wheat. In the spring of 2007, mesosulfuron failed to control Italian ryegrass in several wheat fields. Seed were collected from six fields in two counties and greenhouse studies were conducted to determine response to mesosulfuron and the acetyl-CoA carboxylase (ACCase) inhibitors diclofop and pinoxaden. All populations were resistant to diclofop and cross-resistant to pinoxaden. Five of the six populations were resistant to diclofop, pinoxaden, and mesosulfuron. An additional study with two biotypes confirmed cross-resistance to the ALS inhibitors imazamox, mesosulfuron, and pyroxsulam. Resistance to mesosulfuron was heritable.

Resistance Profile of Diclofop-Resistant Italian Ryegrass (Lolium multiflorum) to ACCase- and ALS-Inhibiting Herbicides in Arkansas, USA

Weed Science ◽  
2008 ◽  
Vol 56 (4) ◽  
pp. 614-623 ◽  
Author(s):  
Yong In Kuk ◽  
Nilda R. Burgos ◽  
Robert C. Scott
Keyword(s):  
Lolium Multiflorum ◽  
Acetolactate Synthase ◽  
Italian Ryegrass ◽  
Cross Resistance ◽  
Southern United States ◽  
Resistance Pattern ◽  
Acetyl Coa Carboxylase ◽  
Wheat Production ◽  
Resistant Accession ◽  
Als Inhibitors

Diclofop-resistant Italian ryegrass is a major weed problem in wheat production. This study aimed to determine the resistance pattern of diclofop-resistant Italian ryegrass accessions from the southern United States to the latest commercialized herbicides for wheat production, pinoxaden and mesosulfuron, and to other acetolactate synthase (ALS) and acetyl-CoA carboxylase (ACCase) inhibitors. Twenty-nine of 36 accessions were resistant to the commercial dose of diclofop. The majority (80%) of diclofop-resistant accessions were also resistant to clodinafop. Of 25 diclofop-resistant accessions, 5 were resistant to pinoxaden. All accessions tested were susceptible to the commercial dose of clethodim and sethoxydim. The cross-resistance pattern of diclofop-resistant Italian ryegrass to other ACCase inhibitors was 20% for pinoxaden and none with clethodim or sethoxydim. One accession was resistant to mesosulfuron but not to diclofop. This mesosulfuron-resistant accession was cross-resistant to sulfometuron but not to imazamox. All diclofop-resistant accessions tested were susceptible to ALS inhibitors, mesosulfuron, sulfometuron, and imazamox. Therefore, diclofop-resistant Italian ryegrass in Arkansas can be controlled with imazamox (in Clearfield wheat) and can mostly be controlled with mesosulfuron and pinoxaden. It could also be controlled by other selective grass herbicides in broadleaf crops.

Italian Ryegrass (Lolium Multiflorum) Management Options in Winter Wheat in Oklahoma

2007 ◽  
Vol 21 (1) ◽  
pp. 151-158 ◽  
Author(s):  
Chad S. Trusler ◽  
Thomas F. Peeper ◽  
Amanda E. Stone
Keyword(s):  
Winter Wheat ◽  
Plant Density ◽  
Control Strategies ◽  
Lolium Multiflorum ◽  
Wheat Yield ◽  
Italian Ryegrass ◽  
Wheat Crop ◽  
Management Options ◽  
Spike Density ◽  
Herbicide Treatments

An experiment was conducted at three sites in central Oklahoma to compare the efficacy of Italian ryegrass management options in no-till (NT) and conventional tillage (CT) winter wheat. The Italian ryegrass management options included selected herbicide treatments, wheat-for-hay, and a rotation consisting of double-crop soybean seeded immediately after wheat harvest, followed by early season soybean, and then by wheat. In continuous wheat, before application of glyphosate or tillage, Italian ryegrass plant densities in mid-September were 12,300 to 15,000 plants/m2in NT plots vs. 0 to 500 plants/m2in CT plots. When applied POST, diclofop controlled more Italian ryegrass than tralkoxydim or sulfosulfuron. In continuous wheat, yields were greater in CT plots than in NT plots at two of three sites. None of the Italian ryegrass management options consistently reduced Italian ryegrass density in the following wheat crop. Of the Italian ryegrass control strategies applied to continuous wheat, three herbicide treatments in NT at Chickasha and all treatments in NT at Perry reduced Italian ryegrass density in the following wheat crop. Italian ryegrass plant density in November and spike density were highly related to wheat yield at two and three sites, respectively. No management options were more profitable than rotation to soybean.

scholarly journals Impact of a Novel W2027L Mutation and Non-Target Site Resistance on Acetyl-CoA Carboxylase-Inhibiting Herbicides in a French Lolium multiflorum Population

Genes ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1838
Author(s):  
Shiv Shankhar Kaundun ◽  
Joe Downes ◽  
Lucy Victoria Jackson ◽  
Sarah-Jane Hutchings ◽  
Eddie Mcindoe
Keyword(s):  
Lolium Multiflorum ◽  
Restriction Enzymes ◽  
Target Site ◽  
Cereal Crops ◽  
Acetyl Coa Carboxylase ◽  
Wild Type ◽  
Resistance Mutations ◽  
Acetyl Coa ◽  
Pcr Product ◽  
Target Site Resistance

Herbicides that inhibit acetyl-CoA carboxylase (ACCase) are among the few remaining options for the post-emergence control of Lolium species in small grain cereal crops. Here, we determined the mechanism of resistance to ACCase herbicides in a Lolium multiflorum population (HGR) from France. A combined biological and molecular approach detected a novel W2027L ACCase mutation that affects aryloxyphenoxypropionate (FOP) but not cyclohexanedione (DIM) or phenylpyraxoline (DEN) subclasses of ACCase herbicides. Both the wild-type tryptophan and mutant leucine 2027-ACCase alleles could be positively detected in a single DNA-based-derived polymorphic amplified cleaved sequence (dPACS) assay that contained the targeted PCR product and a cocktail of two discriminating restriction enzymes. Additionally, we identified three well-characterised I1781L, I2041T, and D2078G ACCase target site resistance mutations as well as non-target site resistance in HGR. The non-target site component endowed high levels of resistance to FOP herbicides whilst partially impacting on the efficacy of pinoxaden and cycloxydim. This study adequately assessed the contribution of the W2027L mutation and non-target site mechanism in conferring resistance to ACCase herbicides in HGR. It also highlights the versatility and robustness of the dPACS method to simultaneously identify different resistance-causing alleles at a single ACCase codon.

Continuous Use of Tribenuron-Methyl Selected for Cross-Resistance to Acetolactate Synthase–inhibiting Herbicides in Wild Mustard (Sinapis arvensis)

Weed Science ◽  
2018 ◽  
Vol 66 (4) ◽  
pp. 424-432 ◽  
Author(s):  
Javid Gherekhloo ◽  
Zahra M. Hatami ◽  
Ricardo Alcántara-de la Cruz ◽  
Hamid R. Sadeghipour ◽  
Rafael De Prado
Keyword(s):  
Winter Wheat ◽  
Acetolactate Synthase ◽  
Dry Weight ◽  
Resistance Mechanisms ◽  
Cross Resistance ◽  
Resistance Level ◽  
Sinapis Arvensis ◽  
Wild Mustard ◽  
Golestan Province ◽  
Tribenuron Methyl

AbstractWild mustard (Sinapis arvensis L.) is a weed that frequently infests winter wheat (Triticum aestivum L.) fields in Golestan province, Iran. Tribenuron-methyl (TM) has been used recurrently to control this species, thus selecting for resistant S. arvensis populations. The objectives were: (1) to determine the resistance level to TM of 14 putatively resistant (PR) S. arvensis populations, collected from winter wheat fields in Golestan province, Iran, in comparison to one susceptible (S) population; and (2) to characterize the resistance mechanisms and the potential evolution of cross-resistance to other classes of acetolactate synthase (ALS)-inhibiting herbicides in three populations (AL-3, G-5, and Ag-Sr) confirmed as being resistant (R) to TM. The TM doses required to reduce the dry weight of the PR populations by 50% were between 2.2 and 16.8 times higher than those needed for S plants. The ALS enzyme activity assays revealed that the AL-3, G-5, and Ag-Sr populations evolved cross-resistance to the candidate ALS-inhibiting herbicides from the sulfonylureas (SU), triazolopyrimidines (TP), pyrimidinyl-thiobenzoates (PTB), sulfonyl-aminocarbonyl-triazolinone (SCT), and imidazolinones (IMI) classes. No differences in absorption, translocation, or metabolism of [14C]TM between R and S plants were observed, suggesting that these non-target mechanisms were not responsible for the resistance. The ALS gene of the R populations contained the Trp-574-Leu mutation, conferring cross-resistance to the SU, SCT, PTB, TP, and IMI classes. The Trp-574-Leu mutation in the ALS gene conferred cross-resistance to ALS-inhibiting herbicides in S. arvensis from winter wheat fields in Golestan province. This is the first TM resistance case confirmed in this species in Iran.

Effect of Wheat Herbicide Carryover on Double-Crop Cotton and Soybean

2012 ◽  
Vol 26 (2) ◽  
pp. 207-212 ◽  
Author(s):  
Timothy L. Grey ◽  
L. Bo Braxton ◽  
John S. Richburg
Keyword(s):  
Winter Wheat ◽  
Sandy Loam ◽  
Residual Activity ◽  
Stand Density ◽  
Acetolactate Synthase ◽  
Italian Ryegrass ◽  
Visible Injury ◽  
Soft Red Winter Wheat ◽  
Herbicide Treatments ◽  
Strip Tillage

In the southeastern United States many farmers double-crop winter wheat with soybean or cotton. However, there is little information about residual injury of herbicides used in wheat to these rotational crops. Experiments were conducted from 2007 to 2008 and 2008 to 2009 in soft red winter wheat to evaluate response of rotational crops of soybean and cotton after application of various acetolactate synthase herbicides in wheat. Pyroxsulam, mesosulfuron, sulfosulfuron, propoxycarbazone, or chlorsulfuron plus metsulfuron at multiple rates were applied to wheat approximately 110 to 120 d before planting rotational crops. Soils were Tift loamy sand at Ty Ty, GA and Faceville sandy loam at Plains, GA. After wheat harvest, soybean (‘Pioneer 97M50’) and cotton (‘DP 0949 B2RF’) were strip-tillage planted and evaluated for injury, stand density, height over time, and yields. For both locations, wheat was tolerant to all herbicide treatments with little to no visible injury 7 to 90 d after application. Pyroxsulam injury was less than sulfosulfuron or mesosulfuron. At recommended use rates, wheat injury was transient with no effect on yield. Double-crop soybean for both locations had no differences in stand establishment for any herbicide treatments. There was significant carryover injury to soybean and cotton for sulfosulfuron applied to wheat for the Faceville sandy loam. There was no effect of herbicide treatment on cotton stand. There was little to no difference in residual activity on rotational crops between pyroxsulam and other wheat herbicides when labeled rates were applied. This is significant as pyroxsulam is used to control Italian ryegrass and wild radish in this region.

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