Cross-resistance to acetyl-CoA carboxylase–inhibiting herbicides conferred by a target-site mutation in perennial ryegrass (Lolium perenne) from Argentina

AbstractIn Argentina, Lolium spp. occur in 40% of winter cereal crops from the Pampas. Several years ago, cases of glyphosate-resistant perennial ryegrass (Lolium perenne L.) were detected, and the use of acetyl-CoA carboxylase (ACCase)-inhibiting herbicides to eradicate these plants has been considered. The aim of this study was to evaluate the sensitivity of a putative pinoxaden-resistant L. perenne population to ACCase-inhibiting herbicides. Around 80% of plants from the putative resistant population survived at a recommended dose of pinoxaden, and they produced viable seeds. The resistance indices (RIs) to pinoxaden were 5.1 and 2.8 for plant survival and seed production, respectively. A single point mutation that conferred a Asp-2078-Gly substitution in ACCase was the source of the resistance. To match the plant control achieved in the susceptible population, the resistant population required 5.4- and 10.4-fold greater doses of clethodim and quizalofop, respectively. RIs for viable seed production when treated with clethodim and quizalofop were 3.3 and 6.6, respectively. The Asp-2078-Gly mutation endowed significant levels of resistance to pinoxaden, clethodim, and quizalofop. For three herbicides, the level of resistance of a pinoxaden-resistant L. perenne population to ACCase inhibitors was evaluated, based on an evaluation of dose response for plant survival and seed production. The RIs were higher for plant survival than for seed production. In Argentina, the selection pressure associated with clethodim and haloxifop preplant application and pinoxaden use on wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) crops, would have favored the propagation of the Asp-2078-Gly mutation with its associated resistance.

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Resistance to Acetolactate Synthase and Acetyl Co-A Carboxylase Inhibitors in North Carolina Italian Ryegrass (Lolium perenne)

Weed Technology ◽

10.1614/wt-d-11-00050.1 ◽

2011 ◽

Vol 25 (4) ◽

pp. 659-666 ◽

Cited By ~ 10

Author(s):

Aman Chandi ◽

Alan C. York ◽

David L. Jordan ◽

Josh B. Beam

Keyword(s):

North Carolina ◽

Lolium Perenne ◽

Acetolactate Synthase ◽

Italian Ryegrass ◽

Cross Resistance ◽

Acetyl Coa Carboxylase ◽

Acetyl Coa ◽

Als Inhibitors

Diclofop-resistant Italian ryegrass is widespread in southwestern North Carolina, and growers have resorted to using acetolactate synthase (ALS) inhibitors such as mesosulfuron and pyroxsulam to control this weed in wheat. In the spring of 2007, mesosulfuron failed to control Italian ryegrass in several wheat fields. Seed were collected from six fields in two counties and greenhouse studies were conducted to determine response to mesosulfuron and the acetyl-CoA carboxylase (ACCase) inhibitors diclofop and pinoxaden. All populations were resistant to diclofop and cross-resistant to pinoxaden. Five of the six populations were resistant to diclofop, pinoxaden, and mesosulfuron. An additional study with two biotypes confirmed cross-resistance to the ALS inhibitors imazamox, mesosulfuron, and pyroxsulam. Resistance to mesosulfuron was heritable.

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Recurrent Selection with Glufosinate at Low Rates Reduces the Susceptibility of a Lolium perenne ssp. multiflorum Population to Glufosinate

Agronomy ◽

10.3390/agronomy10091288 ◽

2020 ◽

Vol 10 (9) ◽

pp. 1288 ◽

Cited By ~ 1

Author(s):

Maor Matzrafi ◽

Sarah Morran ◽

Marie Jasieniuk

Keyword(s):

Lolium Perenne ◽

Recurrent Selection ◽

Cross Resistance ◽

Susceptible Population ◽

Selection Cycle ◽

Herbicide Resistant ◽

Repeated Applications ◽

Resistance To Herbicides ◽

Rate Selection ◽

Low Rate

Repeated applications of herbicides at the labelled rates have often resulted in the selection and evolution of herbicide-resistant weeds capable of surviving the labelled and higher rates in subsequent generations. However, the evolutionary outcomes of recurrent herbicide selection at low rates are far less understood. In this study of a herbicide-susceptible population of Lolium perenne ssp. multiflorum, we assessed the potential for low glufosinate rates to select for reduced susceptibility to the herbicide, and cross-resistance to herbicides with other modes of action. Reduced susceptibility to glufosinate was detected in progeny in comparison with the parental population following three rounds of selection at low glufosinate rates. Differences were mainly observed at the 0.5X, 0.75X, and 1X rates. Comparing the parental susceptible population and progeny from the second and third selection cycle, the percentage of surviving plants increased to values of LD50 (1.31 and 1.16, respectively) and LD90 (1.36 and 1.26, respectively). When treated with three alternative herbicides (glyphosate, paraquat, and sethoxydim), no plants of either the parental or successive progeny populations survived treatment with 0.75X or higher rates of these herbicides. The results of this study provide clear evidence that reduced susceptibility to glufosinate can evolve in weed populations following repeated applications of glufosinate at low herbicide rates. However, the magnitude of increases in resistance levels over three generations of recurrent low-rate glufosinate selection observed is relatively low compared with higher levels of resistance observed in response to low-rate selection with other herbicides (three fold and more).

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Recurrent selection with glufosinate at low rates reduces the susceptibility of a Lolium perenne ssp. multiflorum population to glufosinate

10.1101/2020.07.04.182733 ◽

2020 ◽

Author(s):

Maor Matzrafi ◽

Sarah Morran ◽

Marie Jasieniuk

Keyword(s):

Lolium Perenne ◽

Recurrent Selection ◽

Cross Resistance ◽

Susceptible Population ◽

Selection Cycle ◽

Herbicide Resistant ◽

Repeated Applications ◽

Resistance To Herbicides ◽

Rate Selection ◽

Low Rate

ABSTRACTRepeated applications of herbicides at the labelled rates have often resulted in the selection and evolution of herbicide-resistant weeds capable of surviving the labelled and higher rates in subsequent generations. However, the evolutionary outcomes of recurrent herbicide selection at low rates are far less understood. In this study of an herbicide-susceptible population of Lolium perenne ssp. multiflorum, we assessed the potential for low glufosinate rates to select for reduced susceptibility to the herbicide, and cross-resistance to herbicides with other modes of action. Reduced susceptibility to glufosinate was detected in progeny in comparison with the parental population following three rounds of selection at low glufosinate rates. Differences were mainly observed at the 0.5X, 0.75X, and 1X rates. Comparing the parental susceptible population and progeny from the second and third selection cycle, the percentage of surviving plants increased to values of LD50 (1.31 and 1.16, respectively) and LD90 (1.36 and 1.26, respectively). When treated with three alternative herbicides (glyphosate, paraquat, and sethoxydim), no plants of either the parental or successive progeny populations survived treatment with 0.75X or higher rates of these herbicides. The results of this study provide clear evidence that reduced susceptibility to glufosinate can evolve in weed populations following repeated applications of glufosinate at low herbicide rates. However, the magnitude of increases in resistance levels over three generations of recurrent low-rate glufosinate selection observed is relatively low compared with higher levels of resistance observed in response to low-rate selection with other herbicides (three fold and more).

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Assessment of gene flow using tetraploid genotypes of perennial ryegrass (Lolium perenne L.)

Australian Journal of Agricultural Research ◽

10.1071/ar03156 ◽

2004 ◽

Vol 55 (4) ◽

pp. 389 ◽

Cited By ~ 15

Author(s):

K. V. Cunliffe ◽

A. C. Vecchies ◽

E. S. Jones ◽

G. A. Kearney ◽

J. W. Forster ◽

...

Keyword(s):

Gene Flow ◽

Seed Production ◽

Lolium Perenne ◽

Perennial Ryegrass ◽

Grass Species ◽

Numerical Representation ◽

Pasture Grass ◽

Important Species ◽

Lolium Perenne L ◽

Total Seed

Ryegrass species are among the most important species in sown pastures, turf settings, and weed populations worldwide. Perennial ryegrass (Lolium perenne L.) is an outcrossing, wind-pollinated grass. Recent research has demonstrated the feasibility of developing transgenic perennial ryegrass varieties. In order to model the consequences of gene flow from transgenic grass genotypes in a field situation, the model non-transgenic trait of fertility among autotetraploid genotypes was chosen. Gene flow over distance and direction from a donor plot to surrounding sexually compatible recipient plants was studied. Reproductive isolation was achieved through the fertility barrier that arises between tetraploid and diploid ryegrass genotypes, despite the presence of diploid plants in a meadow situation. Fertility was used as an indication of effective gene flow over distance and direction. Measures of the fertility of recipient plants included total seed production (TSP), floret site utilisation (FSU), and relative fertility of recipient plants as a percentage of those within the donor plot (RF%). A leptokurtic distribution for gene flow was identified, with differences in the rate of decline over distance depending on direction. Simple sequence repeat (SSR) polymorphism was used to identify the paternity of progeny plants. The proportional representation of parents among the progeny was not significantly different from that expected due to the numerical representation of the different donor parent genotypes. The results of this research will have important implications for risk analysis prior to the field release of transgenic ryegrasses, fescues, and other pasture grass species, and for seed production in terms of cultivar purity and optimum isolation distance.

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Cross resistance patterns to acetyl-CoA carboxylase inhibiting herbicides associated with different mutations in Italian ryegrass from China

Crop Protection ◽

10.1016/j.cropro.2020.105479 ◽

2020 ◽

pp. 105479

Author(s):

Pei Zhang ◽

Yangyang Zhang ◽

Xiling Chen ◽

Liyao Dong

Keyword(s):

Italian Ryegrass ◽

Cross Resistance ◽

Acetyl Coa Carboxylase ◽

Acetyl Coa ◽

Resistance Patterns

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Characterization of cross-resistance patterns in acetyl-CoA carboxylase inhibitor resistant wild oat (Avena fatua)

Weed Science ◽

10.1017/s0043174500088925 ◽

1997 ◽

Vol 45 (6) ◽

pp. 750-755 ◽

Cited By ~ 34

Author(s):

Luc Bourgeois ◽

Norm C. Kenkel ◽

Ian N. Morrison

Keyword(s):

Cluster Analysis ◽

Principal Component ◽

Petri Dish ◽

Cross Resistance ◽

Wild Oat ◽

Acetyl Coa Carboxylase ◽

Acetyl Coa ◽

Pairwise Correlation ◽

Resistance Patterns

The purpose of this study was to determine cross-resistance patterns among wild oat lines resistant to acetyl-CoA carboxylase (ACCase) inhibitors and to determine which, if any, cross-resistant type was more common than another. Discriminatory concentrations of two aryloxyphenoxy-propionates (APP) and three cyclohexanediones (CHD) were determined using a petri-dish bioassay. These concentrations were then applied to 82 resistant wild oat lines identified in previous studies. In addition, two resistant standards (UM1 and UM33) and a susceptible standard (UM5) were included in the experiments. Coleoptile lengths expressed as percentages of untreated controls were used to assess the level of resistance to each herbicide. Large variations were observed among wild oat lines and herbicides. However, cluster analysis summarized the relationship between the five herbicides (variables) and the wild oat lines into three main cross-resistance types. Type A included wild oat lines with high resistance to APP herbicides and no or low resistance to CHD herbicides. Types B and C included those with low to moderate resistant and high levels of resistance to all five herbicides, respectively. Type C was the most common cross-resistance type. Relationships among herbicides were determined using pairwise correlation and principal component analysis (PCA). All correlations were high between APP herbicides and between CHD herbicides but not between APP and CHD herbicides. The first two axes of the PCA accounted for 88.4% of the total variance, with the first axis correlated to the CHD herbicides and the second axis correlated to the APP herbicides. In the PCA, wild oat lines were segregated into the three types identified in the cluster analysis. Although CHD and APP herbicides bind at the same region on the ACCase, resistant wild oat lines respond differently to them.

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Target Enzyme-Based Resistance to Clethodim inLolium rigidumPopulations in Australia

Weed Science ◽

10.1614/ws-d-14-00176.1 ◽

2015 ◽

Vol 63 (4) ◽

pp. 946-953 ◽

Cited By ~ 10

Author(s):

Rupinder Kaur Saini ◽

Jenna Malone ◽

Christopher Preston ◽

Gurjeet Gill

Keyword(s):

Effective Control ◽

Cross Resistance ◽

Single Plant ◽

Susceptible Population ◽

Ambient Temperatures ◽

Resistant Population ◽

Rigid Ryegrass ◽

Moderately Resistant ◽

Two Populations ◽

Biomass Reduction

Clethodim resistance was identified in 12 rigid ryegrass populations from winter cropping regions in four different states of Australia. Clethodim had failed to provide effective control of these populations in the field and resistance was suspected. Dose–response experiments confirmed resistance to clethodim and butroxydim in all populations. During 2012, the LD50of resistant populations ranged from 10.2 to 89.3 g ha−1, making them 3 to 34–fold more resistant to clethodim than the susceptible population. Similarly, GR50of resistant population varied from 8 to 37.1 g ha−1, which is 3 to 13.9–fold higher than the susceptible population. In 2013, clethodim-resistant populations were 7.8 to 35.3–fold more resistant to clethodim than the susceptible population. The higher resistance factor in 2013, especially in moderately resistant populations, could have been associated with lower ambient temperatures during the winter of 2013. These resistant populations had also evolved cross-resistance to butroxydim. The resistant populations required 1.3 to 6.6–fold higher butroxydim dose to achieve 50% mortality and 3 to 27–fold more butroxydim for 50% biomass reduction compared to the standard susceptible population. Sequencing of the target-site ACCase gene identified five known ACCase substitutions (isoleucine-1781-leucine, isoleucine-2041-asparagine, aspartate-2078-glycine, and cysteine-2088-arginine, and glycine-2096-alanine) in these populations. In nine populations, multiple ACCase mutations were present in different individuals. Furthermore, two alleles with different mutations were present in a single plant of rigid ryegrass in two populations.

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Variation in Response and Resistance to Glyphosate and Glufosinate in California Populations of Italian Ryegrass (Lolium perennessp.multiflorum)

Weed Science ◽

10.1017/wsc.2017.71 ◽

2017 ◽

Vol 66 (2) ◽

pp. 168-179 ◽

Cited By ~ 6

Author(s):

Elizabeth Karn ◽

Roland Beffa ◽

Marie Jasieniuk

Keyword(s):

Regression Models ◽

Early Stage ◽

Italian Ryegrass ◽

Cross Resistance ◽

Susceptible Population ◽

High Frequencies ◽

Logistic Regression Models ◽

Time Period ◽

Resistant Population ◽

California Population

Reduced control of Italian ryegrass in California with herbicides has raised concerns about the evolution of populations with resistance to multiple herbicides. The goal of this study was to investigate variation among populations in plant response and resistance to glyphosate and glufosinate in Italian ryegrass from vineyards and orchards in northwest California. Population resistance screening using field-collected seed revealed up to 56.9% of individuals surviving glyphosate treatment at 1,678 g ae ha−1, and 53.5% of individuals surviving glufosinate treatment at 2,242 g ai ha−1in the same population. Frequencies of surviving plants within populations varied among screening times, particularly for glufosinate. Treating vegetatively propagated, genetically identical tillers with each herbicide pointed to separate mechanisms of resistance rather than cross-resistance to glyphosate and glufosinate. Dose–response experiments were conducted for each herbicide at two different screening times using a subset of populations, field-collected seed, and 10 herbicide rates. Plant survival and biomass were evaluated for each population at 3 wk after treatment and for plant regrowth 1 wk thereafter. Log-logistic regression models fit to the data were used to estimate LD50, GR50, and RD50values and calculate resistance indices (R/S ratios). Based on LD50values, the most highly resistant population was 14.4- to 19.2-fold more resistant to glyphosate than the most susceptible population tested but only 1.6- to 2.0-fold more resistant to glufosinate than the most susceptible population tested. Levels of resistance to both herbicides varied with screening time period and variable measured. Results indicate high frequencies of glyphosate-resistant plants but an early stage in the evolution of glufosinate resistance in some Italian ryegrass populations of northwest California.

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ACCase-Inhibitor Herbicide Resistance in Downy Brome (Bromus tectorum) in Oregon

Weed Science ◽

10.1614/ws-06-120.1 ◽

2007 ◽

Vol 55 (2) ◽

pp. 91-94 ◽

Cited By ~ 9

Author(s):

Daniel A. Ball ◽

Sandra M. Frost ◽

Larry H. Bennett

Keyword(s):

Seed Production ◽

Herbicide Resistance ◽

Bromus Tectorum ◽

Cross Resistance ◽

Acetyl Coa Carboxylase ◽

Downy Brome ◽

Plant Mortality ◽

Red Fescue ◽

Application Rates ◽

Group 1

In spring 2005, a downy brome population with possible resistance to fluazifop-P, an acetyl-CoA carboxylase (ACCase) inhibitor (group 1) herbicide was found in a commercial creeping red fescue seed production field, near La Grande, OR, where fluazifop-P had been used to control downy brome repeatedly over 7 yr. Greenhouse experiments were conducted to confirm resistance to a number of group 1 herbicides. The suspected resistant downy brome accession was tested for resistance to fluazifop-P and tested for cross-resistance to other aryloxyphenoxy propionate (APP) and cyclohexanedione (CHD) herbicides, including quizalofop-P, sethoxydim, and clethodim. Data recorded included plant-mortality counts and biomass. Tests revealed that the La Grande downy brome accession was highly resistant to fluazifop-P and sethoxydim at all tested rates. The La Grande accession suffered no mortality from fluazifop-P or sethoxydim treatments up to the maximum tested rate of eight times (8×) the labeled recommendation. The La Grande accession was resistant to quizalofop-P and clethodim at the labeled rate or less but was susceptible to application rates higher than the labeled rate. The control downy brome accession was completely susceptible to fluazifop-P, quizalofop-P, and clethodim at all rates and exhibited increasing susceptibility with increasing sethoxydim rate. This pattern of cross-resistance differs from that of a previously reported case of ACCase resistance in downy brome.

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