scholarly journals Arabidopsis SnRK1 negatively regulates phenylpropanoid metabolism via Kelch domain‐containing F‐box proteins

2020 ◽  
Author(s):  
Bin Wang ◽  
Xianhai Zhao ◽  
Yunjun Zhao ◽  
John Shanklin ◽  
Qiao Zhao ◽  
...  
Keyword(s):  
Phenylpropanoid Metabolism ◽  
Kelch Domain

scholarly journals Study on browning mechanism of fresh-cut eggplant (Solanum melongena L.) based on metabolomics, enzymatic assays and gene expression

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Xiaohui Liu ◽  
Aidong Zhang ◽  
Jing Shang ◽  
Zongwen Zhu ◽  
Ye Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fruits And Vegetables ◽  
Solanum Melongena ◽  
Downward Trend ◽  
Glutathione Metabolism ◽  
Phenylpropanoid Metabolism ◽  
Kegg Pathways ◽  
Maximum Value ◽  
Cut Time ◽  
Fresh Cut

AbstractEnzymatic browning is one of the crucial problems compromising the flavor and texture of fresh-cut fruit and vegetables. In this study, an untargeted metabolomics approach based on liquid chromatography-mass spectrometry (LC–MS) was used to explore the browning mechanism in fresh-cut eggplant. Metabolomics studies showed that with the increase of fresh-cut time, the contents of 946 metabolites changed dynamically. The metabolites having the same trend share common metabolic pathways. As an important browning substrate, the content of chlorogenic acid increased significantly, suggesting that may be more important to fresh-cut eggplant browning; all 119 common differential metabolites in 5 min/CK and 3 min/CK contrastive groups were mapped onto 31 KEGG pathways including phenylpropanol metabolism, glutathione metabolism pathway, et al. In physiological experiments, results showed that the Phenylpropanoid-Metabolism-Related enzymes (PAL, C4H, 4CL) were changed after fresh-cut treatment, the activities of three enzymes increased first and then decreased, and reached the maximum value at 5 min, indicating the accumulation of phenolic substances. At the same time, ROS were accumulated when plant tissue damaged by cutting, the activities of related antioxidant enzymes (SOD, APX and CAT) changed dynamically after oxidative damage. SOD and APX content increased significantly and reached the maximum value at 10 min after cutting, and then showed a downward trend. However, CAT activity increased sharply and reached the maximum value within 3 min after cutting, then maintained the same activity, and showed a downward trend after 30 min. These data fully demonstrated that the activities of browning related enzymes and gene expression increased with the prolonging of fresh cutting time. We explained the browning mechanism of fresh-cut eggplant by combining metabolomics and physiology, which may lay the foundation for better understanding the mechanism of browning during the fruits and vegetables during processing.

A R2R3-MYB transcriptional activator LmMYB15 regulates chlorogenic acid biosynthesis and phenylpropanoid metabolism in Lonicera macranthoides

Plant Science ◽  
2021 ◽  
Vol 308 ◽  
pp. 110924
Author(s):  
Ning Tang ◽  
Zhengyan Cao ◽  
Cheng Yang ◽  
Dongsheng Ran ◽  
Peiyin Wu ◽  
...  
Keyword(s):  
Chlorogenic Acid ◽  
Transcriptional Activator ◽  
Phenylpropanoid Metabolism ◽  
Acid Biosynthesis ◽  
Lonicera Macranthoides ◽  
R2r3 Myb

scholarly journals R2R3-MYB transcription factors, StmiR858 and sucrose mediate potato flavonol biosynthesis

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Sen Lin ◽  
Rajesh K. Singh ◽  
Moehninsi ◽  
Duroy A. Navarre
Keyword(s):  
Gene Expression ◽  
Abiotic Stress ◽  
Feedback Loop ◽  
Regulatory Mechanisms ◽  
Phenylpropanoid Metabolism ◽  
Biotic And Abiotic Stress ◽  
Health Promoting ◽  
Transient Overexpression ◽  
R2r3 Myb ◽  
R2r3 Myb Transcription Factors

AbstractFlavonols and other phenylpropanoids protect plants from biotic and abiotic stress and are dietarily desirable because of their health-promoting properties. The ability to develop new potatoes (Solanum tuberosum) with optimal types and amounts of phenylpropanoids is limited by lack of knowledge about the regulatory mechanisms. Exogenous sucrose increased flavonols, whereas overexpression of the MYB StAN1 induced sucrolytic gene expression. Heterologous StAN1 protein bound promoter fragments from sucrolytic genes (SUSY1 and INV1). Two additional MYBs and one microRNA were identified that regulated potato flavonols. Overexpression analysis showed MYB12A and C increased amounts of flavonols and other phenylpropanoids. Endogenous flavonol amounts in light-exposed organs were much higher those in the dark. Expression levels of StMYB12A and C were high in flowers but low in tubers. Transient overexpression of miR858 altered potato flavonol metabolism. Endogenous StmiR858 expression was much lower in flowers than leaves and correlated with flavonol amounts in these organs. Collectively, these findings support the hypothesis that sucrose, MYBs, and miRNA control potato phenylpropanoid metabolism in a finely tuned manner that includes a feedback loop between sucrose and StAN1. These findings will aid in the development of potatoes with phenylpropanoid profiles optimized for crop performance and human health.

Alterations in peroxidase activity and phenylpropanoid metabolism induced by Nacobbus aberrans Thorne and Allen, 1944 in chilli (Capsicum annuum L.) CM334 resistant to Phytophthora capsici Leo.

2010 ◽  
Vol 338 (1-2) ◽  
pp. 399-409 ◽  
Author(s):  
Noé López-Martínez ◽  
Ma. Teresa Colinas-León ◽  
Cecilia B. Peña-Valdivia ◽  
Yolanda Salinas-Moreno ◽  
Patricia Fuentes-Montiel ◽  
...  
Keyword(s):  
Capsicum Annuum ◽  
Peroxidase Activity ◽  
Phytophthora Capsici ◽  
Phenylpropanoid Metabolism ◽  
Capsicum Annuum L ◽  
Nacobbus Aberrans

Tissue distribution of phenylpropanoid metabolism in cotyledons of Raphanus sativus L.

Planta ◽  
1985 ◽  
Vol 164 (4) ◽  
pp. 507-511 ◽  
Author(s):  
D. Strack ◽  
M. Pieroth ◽  
H. Scharf ◽  
V. Sharma
Keyword(s):  
Tissue Distribution ◽  
Raphanus Sativus ◽  
Phenylpropanoid Metabolism ◽  
Raphanus Sativus L

Regulation of phenylpropanoid metabolism by exogenous precursors in axenic cultures of Sphagnum fallax

1995 ◽  
Vol 95 (1) ◽  
pp. 83-90 ◽  
Author(s):  
Susanne Rasmussen ◽  
Gotje Peters ◽  
Hansjorg Rudolph
Keyword(s):  
Phenylpropanoid Metabolism ◽  
Axenic Cultures

scholarly journals Comprehensive Analysis of Glucan Elicitor-Regulated Gene Expression in Tobacco BY-2 Cells Reveals a Novel MYB Transcription Factor Involved in the Regulation of Phenylpropanoid Metabolism

2007 ◽  
Vol 48 (10) ◽  
pp. 1404-1413 ◽  
Author(s):  
Tomonori Shinya ◽  
Ivan Gális ◽  
Tomoko Narisawa ◽  
Mami Sasaki ◽  
Hiroo Fukuda ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Comprehensive Analysis ◽  
Myb Transcription Factor ◽  
Phenylpropanoid Metabolism ◽  
Regulated Gene Expression

scholarly journals Identification of a PGXPP degron motif in dishevelled and structural basis for its binding to the E3 ligase KLHL12

Open Biology ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 200041 ◽  
Author(s):  
Zhuoyao Chen ◽  
Gregory A. Wasney ◽  
Sarah Picaud ◽  
Panagis Filippakopoulos ◽  
Masoud Vedadi ◽  
...  
Keyword(s):  
Ubiquitin Ligase ◽  
Molecular Mechanisms ◽  
Hydrophobic Interactions ◽  
E3 Ubiquitin Ligase ◽  
Structural Basis ◽  
E3 Ligases ◽  
Kelch Domain ◽  
Ring E3 Ligases ◽  
Ring E3 ◽  
New Protein

Wnt signalling is dependent on dishevelled proteins (DVL1-3), which assemble an intracellular Wnt signalosome at the plasma membrane. The levels of DVL1-3 are regulated by multiple Cullin-RING E3 ligases that mediate their ubiquitination and degradation. The BTB-Kelch protein KLHL12 was the first E3 ubiquitin ligase to be identified for DVL1-3, but the molecular mechanisms determining its substrate interactions have remained unknown. Here, we mapped the interaction of DVL1-3 to a ‘PGXPP' motif that is conserved in other known partners and substrates of KLHL12, including PLEKHA4, PEF1, SEC31 and DRD4. To determine the binding mechanism, we solved a 2.4 Å crystal structure of the Kelch domain of KLHL12 in complex with a DVL1 peptide that bound with low micromolar affinity. The DVL1 substrate adopted a U-shaped turn conformation that enabled hydrophobic interactions with all six blades of the Kelch domain β-propeller. In cells, the mutation or deletion of this motif reduced the binding and ubiquitination of DVL1 and increased its stability confirming this sequence as a degron motif for KLHL12 recruitment. These results define the molecular mechanisms determining DVL regulation by KLHL12 and establish the KLHL12 Kelch domain as a new protein interaction module for a novel proline-rich motif.

Overexpression of Kelch domain containing-2 (mKlhdc2) inhibits differentiation and directed migration of C2C12 myoblasts

2006 ◽  
Vol 312 (16) ◽  
pp. 3049-3059 ◽  
Author(s):  
Petra Neuhaus ◽  
Benjamin Jaschinsky ◽  
Sebastian Schneider ◽  
Herbert Neuhaus ◽  
Annelies Wolter ◽  
...  
Keyword(s):  
C2c12 Myoblasts ◽  
Directed Migration ◽  
Kelch Domain

scholarly journals Crystal-contact engineering to obtain a crystal form of the Kelch domain of human Keap1 suitable for ligand-soaking experiments

2013 ◽  
Vol 69 (6) ◽  
pp. 592-596 ◽  
Author(s):  
Stefan Hörer ◽  
Dirk Reinert ◽  
Katja Ostmann ◽  
Yvette Hoevels ◽  
Herbert Nar
Keyword(s):  
Crystal Form ◽  
Kelch Domain ◽  
Contact Engineering ◽  
Crystal Contact
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