scholarly journals Molecular phylogeny of fucoxanthin‐chlorophyll a / c proteins from Chaetoceros gracilis and Lhcq/Lhcf diversity

2021 ◽  
Author(s):  
Minoru Kumazawa ◽  
Hiroyo Nishide ◽  
Ryo Nagao ◽  
Natsuko Inoue‐Kashino ◽  
Jian‐Ren Shen ◽  
...  
Keyword(s):  
Molecular Phylogeny ◽  
Chlorophyll A ◽  
Chaetoceros Gracilis ◽  
C Proteins

scholarly journals Spatio-temporal distribution of microalgae producing chlorophyll and carotenoid pigments in Bali Strait, Indonesia

2018 ◽  
Vol 20 (1) ◽  
pp. 61-67
Author(s):  
ANNA FAUZIAH ◽  
DIETRIECH G BENGEN ◽  
MUJIZAT KAWAROE ◽  
HEFNI EFFENDI ◽  
MAJARIANA KRISANTI
Keyword(s):  
Principal Component Analysis ◽  
Water Resources ◽  
Chlorophyll A ◽  
Correspondence Analysis ◽  
Temporal Distribution ◽  
Principal Component ◽  
Carotenoid Pigments ◽  
Chlorophyll B ◽  
Chaetoceros Gracilis ◽  
Spatio Temporal

Fauziah A, Bengen DG, Kawaroe M, Effendi H, Krisanti M. 2019. Spatio-temporal distribution of microalgae producing chlorophyll and carotenoid pigments in Bali Strait, Indonesia. Biodiversitas 20: 61-67. The Bali Strait waters become a place for various development activities that connect Java Island and Bali Island so that it is thought to have an impact in the life of microalgae that play an important role in the wealth of their water resources. This study aims to explore the spatiotemporal distribution of marine microalgae, which has the potential to produce chlorophyll and carotenoid pigments, as well as their relationship with the environmental characteristics of the Bali Strait waters. The research was conducted at the 5 stations in the morning, midday and afternoon. The data obtained were analyzed using Principal component analysis (PCA) and Correspondence analysis (CA). The results showed that chlorophyll-a, chlorophyll-b and carotenoid were mostly contained by the species Chaetoceros gracilis, Tripos lunula at station 5 (Pang-pang Bay) in the morning, midday and afternoon, and contained by the species Fragilariopsis cylindrus, Thalassiothrix fravenfeldii at station 3 (Ketapang-Gilimanuk ferriage) in the midday and afternoon. Spatially, salinity, ammonia, nitrate, and chlorophyll-a have significantly different values between stations, while temporally the content of chlorophyll-b and carotenoid are influenced by time (in the morning, midday and afternoon).

scholarly journals Respon Pertumbuhan dan Konsentrasi Klorofil–a pada Kultur Mikroalga Chaetoceros gracilis terhadap Perbedaan Suhu Kultur

2021 ◽  
Vol 4 (1) ◽  
pp. 37
Author(s):  
Ni Luh Putu Citra Adriyanti ◽  
I Wayan Arthana ◽  
Widiastuti Widiastuti
Keyword(s):  
Chlorophyll A ◽  
Growth Rates ◽  
Lag Phase ◽  
Growth Trend ◽  
Chlorophyll A Concentration ◽  
Chaetoceros Gracilis ◽  
Different Temperatures ◽  
The Difference ◽  
Doubling Times ◽  
One Way Anova

Microalgae Chaetoceros gracilis is widely used in aquaculture. Its culture highly depends on environmental factors, particularly temperature. This study aimed to examine the growth rate and its growth trend of C. gracilis, also chlorophyll-a concentration at different temperature. cultures were incubated at 25, 30 dan 340C for seven days using Na media with three replicates. Cell density was counted every day using a hemocytometer, whereas the concentration of chlorophyll-a was measured in spectrophotometry method. The differences in growth rates and chlorophyll-a concentration at different temperatures were statistically analyzed with One-Way ANOVA. The difference variables were further tested with the Tukey HSD test. The trend of the growth and duration of C. gracilis at 25 and 30 0C were similar, with the lag phase (three days), logarithmic (two days), stationary (one day), and declination (one day), in contrast at 34 0C that had lower duration and missed the stationary phase. The growth rates of microalgae C. gracilis was insignificantly differences at the temperature of 25 and 30 0C (0.7±0.05 cell/ml/day), but significantly lower at 340C (0.28±0.03 cell/ml/day). The chlorophyll-a concentrations at the temperature of 25 and 30 0C were not significantly different (0.37±0.05 and 0.39±0.03 mg/l), while the chlorophyll-a concentration at 34 0C was significantly lower (0.26±0.04 mg/l). This result indicates the high temperature (34 0C) inhibited the metabolism of C. gracilis as shown by doubling times that slower (2.5 day) than the temperature at 25 and 30 0C. It is concluded that the optimum temperatures for the C. gracilis were 25 and 30 0C, where as 340C was the inhibited temperature.

scholarly journals Chemical compositon of three microalgae species for possible use in mariculture

2007 ◽  
Vol 50 (3) ◽  
pp. 461-467 ◽  
Author(s):  
Alfredo Matos Moura Junior ◽  
Egídio Bezerra Neto ◽  
Maria Luise Koening ◽  
Enide Eskinazi Leça
Keyword(s):  
Chlorophyll A ◽  
Soluble Carbohydrates ◽  
Exponential Growth Phase ◽  
Constant Illumination ◽  
Cylindrotheca Closterium ◽  
Cellular Density ◽  
Chaetoceros Gracilis ◽  
Almost All ◽  
Total Amino Acids ◽  
Made In

The purpose of this study was to determine the chemical composition of Chaetoceros gracilis, Cylindrotheca closterium and Tetraselmis gracilis, species frequently used as food for aquaculture in the northeast of Brazil. The species were grown in f/2 medium, in carboys, aerated from one liter, with constant illumination and analyses were made in late exponential growth phase. Cellular density (8,447 x 10 6 cell.L-1), soluble carbohydrates (0.10±0.01 mg.L-1) and magnesium (14.78±0.08 mg.L-1) were the highest in C. gracilis. C . closterium had the highest amount of chlorophyll a (13.76±1.11 mg.L-1), soluble proteins (0.62±0.04 mg.L-1), total amino acids (23.82±0.84 mg .L-1), nitrate (0.36±0.02 mg.L-1), sodium (0.46±0.05 mg.L-1) and phosphorus (2.80±0.38 mg.L-1). Both the species had potassium levels of 0.02 mg.L-1 and sulfur levels of 0.03 mg.L-1. T. gracilis had the lowest values in almost all the analyzed variables, except for chlorophyll a. Therefore, among the analyzed species and tested conditions, C. closterium represented the best nutritional option for aquaculture projects.

scholarly journals Proteases are associated with a minor fucoxanthin chlorophyll a/c-binding protein from the diatom, Chaetoceros gracilis

2012 ◽  
Vol 1817 (12) ◽  
pp. 2110-2117 ◽  
Author(s):  
Ryo Nagao ◽  
Tatsuya Tomo ◽  
Eri Noguchi ◽  
Takehiro Suzuki ◽  
Akinori Okumura ◽  
...  
Keyword(s):  
Chlorophyll A ◽  
Binding Protein ◽  
Chaetoceros Gracilis ◽  
A Minor

scholarly journals Effects of iron availability on pigment signature and biogenic silica production in the coastal diatom Chaetoceros gracilis

2013 ◽  
Vol 4 (1) ◽  
pp. 20 ◽  
Author(s):  
Haimanti Biswas ◽  
Debasmita Bandyopadhyaya
Keyword(s):  
Chlorophyll A ◽  
Biogenic Silica ◽  
Light Harvesting ◽  
Silica Content ◽  
Supplementary Information ◽  
Iron Availability ◽  
Iron Stress ◽  
Iron Supply ◽  
Chaetoceros Gracilis ◽  
Pigment Ratios

The effects of iron availability on pigment signature and biogenic silica production were investigated for the first time in the coastal diatom Chaetoceros gracilis (isolated from the SW coast of the Bay of Bengal, India). Results revealed that increase in iron supply considerably increases chlorophyll a based specific growth rates, whereas, it decreases the values of diatoxanthin index and photoprotective to light harvesting pigment ratios. It is likely that, under iron stress C. gracilis activated a strong photoprotection mechanism by maximising the conversion of diadinoxanthin to diatoxanthin and by increasing overall amount of photoprotective pigments relative to light harvesting pigments. The xanthophyll cycle comprising of diadinoxanthin and diatoxanthin seems to be the principal photoprotective mechanism in C. gracilis and is consistent with some earlier studies. It is suggested that under iron limited conditions the diatoxanthin index and the ratio of photoprotective to light harvesting pigments can be used as physiological markers for iron stress in C. gracilis. The ratios of biogenic silica to chlorophyll a were considerably decreased with increasing iron supply indicating the possibility of reduced cellular silica content under iron enriched conditions; however, supplementary information is required to confirm this observation.

High-Resolution electron crystallography of the light-harvesting chlorophyll-a/b protein complex from chloroplast membranes

1990 ◽  
Vol 48 (1) ◽  
pp. 610-610
Author(s):  
Werner Kühlbrandt ◽  
Da Neng Wang ◽  
K.H. Downing
Keyword(s):  
High Resolution ◽  
Electron Diffraction ◽  
Chlorophyll A ◽  
Protein Complex ◽  
Structural Integrity ◽  
Light Harvesting ◽  
Lhc Ii ◽  
Diffraction Patterns ◽  
Difference Fourier ◽  
2D Crystals

The light-harvesting chlorophyll-a/b protein complex (LHC-II) is the most abundant membrane protein in the chloroplasts of green plants where it functions as a molecular antenna of solar energy for photosynthesis. We have grown two-dimensional (2d) crystals of the purified, detergent-solubilized LHC-II . The crystals which measured 5 to 10 μm in diameter were stabilized for electron microscopy by washing with a 0.5% solution of tannin. Electron diffraction patterns of untilted 2d crystals cooled to 130 K showed sharp spots to 3.1 Å resolution. Spot-scan images of 2d crystals were recorded at 160 K with the Berkeley microscope . Images of untilted crystals were processed, using the unbending procedure by Henderson et al . A projection map of the complex at 3.7Å resolution was generated from electron diffraction amplitudes and high-resolution phases obtained by image processing .A difference Fourier analysis with the same image phases and electron diffraction amplitudes recorded of frozen, hydrated specimens showed no significant differences in the 3.7Å projection map. Our tannin treatment therefore does not affect the structural integrity of the complex.

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