A novel cytological technique for bacterial detection on canine skin

: The prevention and treatment of various infections caused by microbes through antibiotics are becoming less effective due to antimicrobial resistance. Researches are focused on antimicrobial nanomaterials to inhibit bacterial growth and destroy the cells, to replace conventional antibiotics. Recently, carbon dots (C-Dots) become attractive candidates for a wide range of applications, including the detection and treatment of pathogens. In addition to low toxicity, ease of synthesis and functionalization, and high biocompatibility, C-Dots show excellent optical properties such as multi-emission, high brightness, and photostability. C-Dots have shown great potential in various fields, such as biosensing, nanomedicine, photo-catalysis, and bioimaging. This review focuses on the origin and synthesis of various C-Dots with special emphasis on bacterial detection, the antibacterial effect of CDots, and their mechanism.

Download Full-text

Differentially Enhancing Effects of Long-term Treatment with Serrazyme, Boswellia and Pine on Seminal Bacterial Detection in Patients with Chronic Bacterial or Inflammatory Prostatitis, Probably Related to Several Degrees of Bacterial Adherence

Current Clinical Pharmacology ◽

10.2174/1574884713666180803114654 ◽

2018 ◽

Vol 13 (3) ◽

pp. 183-189

Author(s):

Enzo Vicari ◽

Giulia Malaguarnera ◽

Beatrice Ornella Vicari ◽

Mario Salmeri ◽

Michele Salemi ◽

...

Keyword(s):

Term Treatment ◽

Bacterial Adherence ◽

Bacterial Detection ◽

Long Term Treatment

Download Full-text

Biosensors Based on Isothermal DNA Amplification for Bacterial Detection in Food Safety and Environmental Monitoring

Sensors ◽

10.3390/s21020602 ◽

2021 ◽

Vol 21 (2) ◽

pp. 602

Author(s):

Sandra Leonardo ◽

Anna Toldrà ◽

Mònica Campàs

Keyword(s):

Food Safety ◽

Environmental Monitoring ◽

Rolling Circle Amplification ◽

Dna Amplification ◽

Isothermal Amplification ◽

In Situ Monitoring ◽

Strand Displacement ◽

Strand Displacement Amplification ◽

Bacterial Detection ◽

Efficient Detection

The easy and rapid spread of bacterial contamination and the risk it poses to human health makes evident the need for analytical methods alternative to conventional time-consuming laboratory-based techniques for bacterial detection. To tackle this demand, biosensors based on isothermal DNA amplification methods have emerged, which avoid the need for thermal cycling, thus facilitating their integration into small and low-cost devices for in situ monitoring. This review focuses on the breakthroughs made on biosensors based on isothermal amplification methods for the detection of bacteria in the field of food safety and environmental monitoring. Optical and electrochemical biosensors based on loop mediated isothermal amplification (LAMP), rolling circle amplification (RCA), recombinase polymerase amplification (RPA), helicase dependent amplification (HDA), strand displacement amplification (SDA), and isothermal strand displacement polymerisation (ISDPR) are described, and an overview of their current advantages and limitations is provided. Although further efforts are required to harness the potential of these emerging analytical techniques, the coalescence of the different isothermal amplification techniques with the wide variety of biosensing detection strategies provides multiple possibilities for the efficient detection of bacteria far beyond the laboratory bench.

Download Full-text

The potential of spectral and hyperspectral-imaging techniques for bacterial detection in food: A case study on lactic acid bacteria

Talanta ◽

10.1016/j.talanta.2016.02.059 ◽

2016 ◽

Vol 153 ◽

pp. 111-119 ◽

Cited By ~ 17

Author(s):

Giorgia Foca ◽

Carlotta Ferrari ◽

Alessandro Ulrici ◽

Giorgia Sciutto ◽

Silvia Prati ◽

...

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Hyperspectral Imaging ◽

Imaging Techniques ◽

Bacterial Detection

Download Full-text

Rapid Bacterial Detection during Endodontic Treatment

Journal of Dental Research ◽

10.1177/0022034517691723 ◽

2017 ◽

Vol 96 (6) ◽

pp. 626-632 ◽

Cited By ~ 9

Author(s):

D.B. Herzog ◽

N.A. Hosny ◽

S.A. Niazi ◽

G. Koller ◽

R.J. Cook ◽

...

Keyword(s):

Endodontic Treatment ◽

Bacterial Detection

Download Full-text

A simple nanostructured impedimetric biosensor based on clavanin a peptide for bacterial detection

Sensors and Actuators B Chemical ◽

10.1016/j.snb.2017.09.153 ◽

2018 ◽

Vol 255 ◽

pp. 3267-3274 ◽

Cited By ~ 4

Author(s):

Alberto G. Silva Junior ◽

Maria D.L. Oliveira ◽

Idjane S. Oliveira ◽

Reginaldo G. Lima-Neto ◽

Sandra R. Sá ◽

...

Keyword(s):

Bacterial Detection ◽

Impedimetric Biosensor

Download Full-text

Evaluation of the CosmosID Bioinformatics Platform for Prosthetic Joint-Associated Sonicate Fluid Shotgun Metagenomic Data Analysis

Journal of Clinical Microbiology ◽

10.1128/jcm.01182-18 ◽

2018 ◽

Vol 57 (2) ◽

Cited By ~ 8

Author(s):

Qun Yan ◽

Yu Mi Wi ◽

Matthew J. Thoendel ◽

Yash S. Raval ◽

Kerryl E. Greenwood-Quaintance ◽

...

Keyword(s):

Antibiotic Resistance ◽

Metagenomic Data ◽

Metagenomic Sequencing ◽

Antibacterial Resistance ◽

Sequencing Data ◽

Bacterial Detection ◽

Shotgun Metagenomic Sequencing ◽

Prosthetic Joint ◽

Validation Set ◽

Fluid Culture

ABSTRACT We previously demonstrated that shotgun metagenomic sequencing can detect bacteria in sonicate fluid, providing a diagnosis of prosthetic joint infection (PJI). A limitation of the approach that we used is that data analysis was time-consuming and specialized bioinformatics expertise was required, both of which are barriers to routine clinical use. Fortunately, automated commercial analytic platforms that can interpret shotgun metagenomic data are emerging. In this study, we evaluated the CosmosID bioinformatics platform using shotgun metagenomic sequencing data derived from 408 sonicate fluid samples from our prior study with the goal of evaluating the platform vis-à-vis bacterial detection and antibiotic resistance gene detection for predicting staphylococcal antibacterial susceptibility. Samples were divided into a derivation set and a validation set, each consisting of 204 samples; results from the derivation set were used to establish cutoffs, which were then tested in the validation set for identifying pathogens and predicting staphylococcal antibacterial resistance. Metagenomic analysis detected bacteria in 94.8% (109/115) of sonicate fluid culture-positive PJIs and 37.8% (37/98) of sonicate fluid culture-negative PJIs. Metagenomic analysis showed sensitivities ranging from 65.7 to 85.0% for predicting staphylococcal antibacterial resistance. In conclusion, the CosmosID platform has the potential to provide fast, reliable bacterial detection and identification from metagenomic shotgun sequencing data derived from sonicate fluid for the diagnosis of PJI. Strategies for metagenomic detection of antibiotic resistance genes for predicting staphylococcal antibacterial resistance need further development.

Download Full-text