scholarly journals Automation of blood component preparation from whole blood collections

Vox Sanguinis ◽  
2014 ◽  
Vol 107 (1) ◽  
pp. 10-18 ◽  
Author(s):  
J. Cid ◽  
L. Magnano ◽  
M. Lozano
Keyword(s):  
Whole Blood ◽  
Blood Component

Prestorage Leukocyte Depletion with In-Line Filtration of Whole Blood in Comparison with Blood Component Leukocyte Depletion

Vox Sanguinis ◽  
1995 ◽  
Vol 69 (3) ◽  
pp. 201-205 ◽  
Author(s):  
J. Riggert ◽  
G. Simson ◽  
J. Dittmann ◽  
M. Köhler
Keyword(s):  
Whole Blood ◽  
Blood Component ◽  
Leukocyte Depletion

scholarly journals 1792. Viral DNA Loads in Various Blood Components of Patients with EBV-Positive T/NK Cell Lymphoproliferative Diseases

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S660-S661
Author(s):  
Jun-ichi Kawada ◽  
Yasuko Kamiya ◽  
Akihisa Sawada ◽  
Keiji Iwatsuki ◽  
Koji Izustu ◽  
...  
Keyword(s):  
Disease Activity ◽  
Infectious Mononucleosis ◽  
Whole Blood ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Nk Cell ◽  
Inactive Disease ◽  
Blood Component ◽  
Blood Components ◽  
Ebv Dna

Abstract Background Epstein–Barr virus (EBV) is associated with T- and NK-cell lymphoproliferative disorders (EBV T/NK-LPD). For diagnosis of EBV T/NK-LPD, quantification of EBV DNA loads in peripheral blood by real-time PCR has been widely used. However, optimal blood components and cut-off values for diagnosis were not fully evaluated. Methods Fifty-nine patients with EBV T/NK-LPD including chronic active EBV infection (CAEBV), severe mosquito bite allergy, hydroa vacciniforme-like lymphoproliferative disorder (HV), and EBV- hemophagocytic lymphohistiocytosis (EBV-HLH) were enrolled. EBV DNA loads were compared among disease categories in each blood component from the same whole blood sample. The association between EBV DNA loads and disease activity were evaluated in CAEBV patients. Furthermore, the diagnostic cut-off value for EBV DNA loads in whole blood from CAEBV patients as compared with infectious mononucleosis patients was determined. Results EBV DNA loads in whole blood and peripheral blood mononuclear cells (PBMCs) were not significantly different among disease categories, whereas EBV DNA loads in plasma were significantly higher in EBV- HLH patients than in HV patients. EBV DNA loads in whole blood and PBMCs showed strong correlation (Figure 1). EBV DNA loads in plasma were significantly higher in CAEBV patients with active disease than in those with inactive disease (median: 104.5 IU/mL vs. 100.8 IU/mL, P < 0.001) (Figure 2). Diagnostic cut-off values for whole blood EBV DNA loads of CAEBV patients as compared with those of infectious mononucleosis was 104.2 ( = 15,800) IU/mL (Figure 3). Conclusion Measuring EBV DNA loads in whole blood can be considered as initial evaluation for diagnosis of EBV T/NK-LPD. EBV DNA loads in plasma are more closely related to disease activity of CAEBV than EBV DNA loads in whole blood and PBMCs. Disclosures All authors: No reported disclosures.

Military Blood Banking: Criteria For Storage, Refrigeration, Transport, and Other Aspects Required In Whole Blood and Blood Component Logistics

1976 ◽  
Vol 141 (3) ◽  
pp. 157-162 ◽  
Author(s):  
Frank R. Camp ◽  
Dailey W. McPeak ◽  
Turman E. Allen
Keyword(s):  
Whole Blood ◽  
Blood Component ◽  
Blood Banking

New Options for Multicomponent Collection (MCC) with the Latest Cell Separators

1998 ◽  
Vol 21 (6_suppl) ◽  
pp. 26-30 ◽  
Author(s):  
R. Bruni ◽  
M. Valbonesi ◽  
R. Frisoni ◽  
G. Lercari ◽  
M.R. Ruzzenenti ◽  
...  
Keyword(s):  
Health Care ◽  
Whole Blood ◽  
Total Content ◽  
Blood Component ◽  
Red Cell ◽  
Preliminary Results ◽  
Original Configuration

Recently, several new cell separators have become available for high platelet low WBC contamination thrombocytapheresis. In their original configuration, these apparatuses can only collect plasma along with platelets, while no concurrent red cell collection can be carried out. In this study, we present the preliminary results of plasma and/or erythrothrombocytapheresis after the adaptation of the lines of two different cell separators, namely the Fresenius AS 204 and the Baxter Health Care Amicus. In addition, the preliminary results of multicomponent collection using the Dideco Excel will be presented; in the latter a system of plasma recirculation was introduced, to increase the purity and quality of the platelet product. The results of these studies show that erythrothrombocytapheresis can be carried out with all these apparatuses, but only with Dideco-Excel and the Fresenius AS 204 the procedure is totally automated. There are marginal differences in the quality of the RBC product in terms of percent hematocrit, from 65% with the Excel and AS 204 to ∼ 75% with the Amicus and in terms total content of hemoglobin which averages 58 g per RBC concentrate when RBC collection is carried out during the processing of ∼ 420 ml of whole blood. In terms of platelet collection, the differences are presently moderate, as the platelet yield and WBC contamination are not affected by the concurrent RBC collection. The average platelet yield was 3.98-4.1, and 4.01x1011 and the total WBC contamination was 1.2-2.9, and 1.9X105 rerspectively with the Amicus, the AS 204 and the Excel. These represent a new step in the direction of total apheresis for blood and blood component procurement.

Comparison of blood component preparation methods from whole blood bags based on buffy coat extraction

2007 ◽  
Vol 36 (3) ◽  
pp. 243-247 ◽  
Author(s):  
Joan Cid ◽  
Montse Claparols ◽  
Asunción Pinacho ◽  
José Manuel Hernández ◽  
Pilar Ortiz ◽  
...  
Keyword(s):  
Whole Blood ◽  
Buffy Coat ◽  
Blood Component ◽  
Preparation Methods

scholarly journals Pattern of blood component cross-matching and their utilization in a tertiary care hospital of Jammu region

2018 ◽  
Vol 6 (4) ◽  
pp. 1337 ◽  
Author(s):  
Irm Yasmeen ◽  
Ibrar Ahmed ◽  
Meena Sidhu
Keyword(s):  
Whole Blood ◽  
Tertiary Care ◽  
Blood Component ◽  
Care Hospital ◽  
Blood Components ◽  
Cross Sectional ◽  
Inappropriate Use ◽  
Wide Range ◽  
Surgical Conditions ◽  
Cross Matching

Background: Transfusion of donated blood remains the mainstay of treatment for a wide range of medical and surgical conditions. Although it can save life, but transfusion of blood is not without risk. Clinicians should cautiously assess the appropriateness of indications before requesting various blood components thereby preventing misuse of blood and unnecessary exposure of patient to various transfusion transmitted infections and antibodies production. This study was conducted to determine the pattern of whole blood (WB) and blood component cross-matching and their utilization and to minimize the inappropriate use of blood and its components.Methods: This cross-sectional prospective study was performed at SMGS Hospital Blood Bank, Jammu from April 2016 to September 2016. The requisition forms were analysed at the reception counter and inside the pre-transfusion testing laboratory for any error. The department wise utilization of blood and its components, Crossmatching to transfusion (C/T) ratio, transfusion probability (T%) and transfusion index (TI) were calculated.Results: A total of 14376 requests for cross-matching of blood and its components were received. All the units were cross-matched. Out of these, 12766(88.8%) units of blood and its components were issued to various departments. The most common indication for using packed red cells and whole blood was anemia and bleeding (APH/PPH/Trauma). The total C/T Ratio, transfusion probability (T%) and Transfusion index(TI)  of various blood components were 1.12:1, 88.8% and 0.88 respectively.Conclusions: Our study indicates efficient usage of blood and its component. However, awareness is still needed amongst the clinicians and residents to ensure the appropriate use of blood and its components in the future as well. Hospital transfusion committee has to develop transfusion guidelines and subsequent implementation of such guidelines to assure effective blood utilization. MSBOS (maximum surgical blood ordering schedule) should be formulated for elective procedures with regular auditing, feedback, and modifications to improve blood ordering and utilization.

scholarly journals Flow Properties of Modified Packed Red Cells

1987 ◽  
Vol 15 (4) ◽  
pp. 407-410 ◽  
Author(s):  
V. A. Lovric ◽  
M. Schuller ◽  
G. Rose ◽  
J. Raftos ◽  
M. Stewart ◽  
...  
Keyword(s):  
Blood Flow ◽  
Centrifugal Force ◽  
Whole Blood ◽  
Red Cells ◽  
Free Flow ◽  
Blood Component ◽  
Flow Properties ◽  
Blood Storage ◽  
Duration Of Storage

We investigated free gravitational blood flow of modified packed red cells. Variables affecting free flow included final haematocrit, duration of blood storage at 4–6°C and the centrifugal force required in preparation of this blood component. Irrespective of the duration of storage, free blood flow of modified packed red cells was not different from whole blood, provided the haematocrit did not exceed 0.63.

scholarly journals Blood Component Therapy

2018 ◽  
Vol 9 (2) ◽  
pp. 142-147
Author(s):  
Shanaz Karim ◽  
Ehteshamul Hoque ◽  
Md Mazharul Hoque ◽  
Syeda Masooma Rahman ◽  
Kashfia Islam
Keyword(s):  
Red Blood Cells ◽  
Whole Blood ◽  
Blood Cells ◽  
Fresh Frozen Plasma ◽  
Blood Component ◽  
Packed Red Blood Cells ◽  
Medical College ◽  
Blood Component Therapy ◽  
Fresh Frozen ◽  
Cellular Components

Transfusion medicine has undergone advancements since its initiation in the early 20th century. One of these was the discovery that blood can be divided into individual components and delivered separately. Today, blood transfusions nearly always consist of the ad-ministration of 1 or more components of blood. Whole blood transfusion is now limited to situations involving massive resuscitation (trauma ) The most familiar cellular components include packed red blood cells (PRBC), washed PRBC, leukoreduced PRBC and pooled or aphaeresis platelets. Plasma products such as FFP or cryoprecipitate, ant hemophilic factor (CRYO). The transfusion of red blood cells (RBCs), platelets, fresh-frozen plasma (FFP), and cryoprecipitate has the potential of improving clinical outcomes in perioperative and peripartum settings. These benefits include improved tissue oxygenation and decreased bleeding. However, transfusions are not without risks or costs. With the advent of blood component therapy, each unit of whole blood collected serves the specific needs of several, rather than a single patient.Anwer Khan Modern Medical College Journal Vol. 9, No. 2: Jul 2018, P 142-147

Prestorage Leukocyte Depletion with In-Line Filtration of Whole Blood in Comparison with Blood Component Leukocyte Depletion

Vox Sanguinis ◽  
1995 ◽  
Vol 69 (3) ◽  
pp. 201-205
Author(s):  
J. Riggert ◽  
G. Simson ◽  
J. Dittmann ◽  
M. Köhler
Keyword(s):  
Whole Blood ◽  
Blood Component ◽  
Leukocyte Depletion

Novel Initiation Mechanism of Blood Coagulation by Intravascular Tissue Factor.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 3967-3967
Author(s):  
Bernd Engelmann
Keyword(s):  
Plasma Membrane ◽  
Tissue Factor ◽  
Whole Blood ◽  
Thrombin Generation ◽  
Clinical Chemistry ◽  
Sandwich Elisa ◽  
Blood Component ◽  
Potential Contribution ◽  
Fibrin Formation ◽  
Activated Platelets

Abstract Bernd Engelmann Vascular Biology and Hemostasis, Inst. of Clinical Chemistry, Ludwig-Maximilians-Universität, Munich, Germany Following addition of fibrillar collagen to whole blood in order to mimick the starting process of hemostasis, we unexpectedly observed that tissue factor (TF), the central initiator of coagulation, was exposed within 3–5 min in association with CD15 and CD14 positive blood cells. A series of experiments revealed that the TF presentation was restricted to conjugates of neutrophils (and monocytes) with platelets. To verify the source of the TF, isolated neutrophils and platelets were evaluated for the presence of TF. Using a double sandwich Elisa, the washed platelets were found to contain TF. Conversely, TF was undetectable in the neutrophils. When searching for the intraplatelet location of TF by immunoelectron microscopy (IEM), TF was observed to reside in the alpha-granules and in the surface connected system. No TF was present in the cytoplasma and the dense granules. In response to activation, platelet TF was translocated to the cell surface by fusion of the alpha-granules with the plasma membrane. The externalized TF was found to cluster on platelet filopodia. Inspection of rapidly isolated buffy coat preparations confirmed the absence of TF from the neutrophils. Stimulation of TF-dependent factor Xa formation by the activated platelets was markedly amplified by the isolated neutrophils. This required neutrophil-platelet conjugate formation, as evident from inhibition by antibodies targeting PSGL-1 and CD18. To assess whether the TF triggered coagulation was connected to the platelet recruitment, we evaluated the participation of the ADP system. Disrupting the interaction of ADP with its platelet receptors P2Y12 and P2Y1 suppressed the TF activity in the neutrophil-platelet conjugates. Since the TF exposing filopodia represent preferential sites for the formation of microparticles (MP), we isolated the total pool of circulating MP from whole blood, known to be mainly derived from the platelets. Then, the MP were separated by cell sorting. In MP positive for the platelet specific CD42b, TF could be detected and quantified by western blotting and Elisa. Moderate increases in MP number excessively stimulated blood based TF activity in the presence of platelets and in whole blood. Since activated platelets are known to secrete tissue factor pathway inhibitor (TFPI), an anti-TFPI antibody targeting the Kunitz-2 domain of TFPI was included into the suspensions of the activated platelets. Thereby the TF activity of the isolated platelets was enforced, while the activity in the presence of the neutrophils remained unaffected, suggesting that TFPI partially masks the functional competence of the platelet TF. The potential contribution of platelet-collagen interactions for the activation of coagulation in vivo was analyzed by injecting collagen into the venous blood of mice. Local fibrin formation was documented in pulmonary vessels by EM, and systemic thrombin generation was revealed by increased thrombin-antithrombin complexes. In mice deficient for the P2Y1 ADP receptor, the thrombin generation was markedly reduced, indicating a basic role for the platelet-triggered coagulation during thrombus growth. In conclusion, the intravascular tissue factor enables the entire coagulation system to proceed on the plasma membrane of a single blood component, the surface of the activated platelets. Consequently the coagulation start can be regulated within the platelet aggregate, allowing fibrin formation to be flexibly adjusted to the size of the thrombus and the duration of its development.

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