scholarly journals Taxonomic identity of Crepidiastrum ×nakaii recorded on Hongdo Island

2021 ◽  
Vol 51 (3) ◽  
pp. 198-204
Author(s):  
Young-Jong JANG ◽  
Byoung-Hee CHOI
Keyword(s):  
Geographical Distribution ◽  
Related Species ◽  
Internal Transcribed Spacer ◽  
Morphological Traits ◽  
Its Sequences ◽  
Flowering Period ◽  
Morphological Examination

The plant known as “Hong-do-go-deul-ppae-gi” on Hongdo Island, belonging to the genus Crepidiastrum, has been recorded as C. ×nakaii, a hybrid between C. denticulatum and C. platyphyllum. During a survey of the flora of Hongdo Island, we reexamined the taxonomic identity of “Hong-do-go-deul-ppae-gi”. The morphological traits, geographical distribution, and internal transcribed spacer (ITS) sequences of “Hong-do-go-deul-ppae-gi” were compared to related species within the genus. A morphological examination revealed that the plant was similar to C. lanceolatum in that it has subshrub stems with caudex stout, 8 inner involucrals bracts, and 10 to 12 florets. In contrast, similarities to C. denticulatum were observed in terms of radical leaves deciduous in the flowering period, cauline leaves membranous with acute serrations, and beaked achenes. Furthermore, C. denticulatum and C. lanceolatum grew on Hongdo Island and nearby areas, but C. platyphyllum was not distributed in these areas. The results of morphological and distributional investigations revealed that the Hongdo Island plant should be regarded as C. ×muratagenii, a hybrid between C. denticulatum and C. lanceolatum, not C. ×nakaii, which is a hybrid between C. denticulatum and C. platyphyllum. However, the resolution of the ITS sequences was insufficient such that we could not separate the examined species.

Analysis of Genetic Relationships in Germplasms of Mugua in China Revealed by Internal Transcribed Spacer and its Taxonomic Significance Analysis of Genetic Relationships in Germplasms of Mugua in China Revealed by Internal Transcribed Spacer and its Taxonomic Signifi cance

2010 ◽  
Vol 65 (7-8) ◽  
pp. 495-500 ◽  
Author(s):  
Bangxing Han ◽  
Huasheng Peng ◽  
Qin Yao ◽  
Yang Zhou ◽  
Ming’en Cheng ◽  
...  
Keyword(s):  
Genetic Distance ◽  
Related Species ◽  
Internal Transcribed Spacer ◽  
Genetic Relationships ◽  
Distance Matrix ◽  
Its Sequences ◽  
Taxonomic Significance ◽  
Significance Analysis ◽  
Its Analysis ◽  
Genetic Distance Matrix

Genetic relationships were studied among eight species of three taxa in the genus Chaenomeles by nuclear ribosomal internal transcribed spacer (ITS) analysis. A genetic distance matrix based on ITS sequences was estimated according to the formula of Kimura-2 parameter and a neighbour-joining phenogram, which were obtained with Clustalx4.1 software. The results showed that the germplasms of Mugua originate from Ch. speciosa (Sweet) Nakai, not including Ch. sinensis (Thouin) Kochne and Ch. cathayensis (Hemsl.) Schneid. The results also showed that ‘Yao Mugua’ and ‘Ornamental Mugua’ are the most distantly related species in germplasms.

scholarly journals Cortinarius alpinus as an example for morphological and phylogenetic species concepts in ectomycorrhizal fungi

Sommerfeltia ◽  
2008 ◽  
Vol 31 (1) ◽  
pp. 161-177 ◽  
Author(s):  
U. Peintner
Keyword(s):  
Ectomycorrhizal Fungi ◽  
Related Species ◽  
Dry Weight ◽  
Species Concepts ◽  
Its Sequences ◽  
Phylogenetic Species ◽  
Closely Related Species ◽  
Rdna Its ◽  
Rdna Its Sequences ◽  
Distinguishing Character

Cortinarius alpinus as an example for morphological and phylogenetic species concepts in ectomycorrhizal fungiExtensive morphological and molecular analyses of closely related species from alpine, subalpine and montane habitats should enable a comparison of ecological, morphological and phylogenetic species concepts in ectomycorrhizal mushrooms. One fundamental question of this study was whether alpine species really exist, and which criteria, besides the specific habitat, could reliably be used for the de-limitation of such taxa. For this reason, 56 rDNA ITS sequences were generated or downloaded from GenBank for 10 closely related species of Cortinarius subgenus Myxacium, section Myxacium. Several collections were sequenced for each of the following taxa: Cortinarius absarokensis, C. alpinus, C. favrei, C. fennoscandicus, C. grallipes, C. mucosus, C. muscigenus, C. septentrionalis, C. trivialis and C. vernicosus. Moreover, spore statistics were carried out for 38 collections of alpine and subalpine taxa. These data provide clear evidence for C. favrei being a synonym of C. alpinus. C. absarokensis and C. alpinus can clearly be delimited based on pileus diameter and average dry weight per basidiome, even in overlapping habitats, but spore size and shape is not a good distinguishing character. Phylograms have very short branches, and base differences between ITS sequences are generally very low in this group, and give no resolution for the included taxa of this section. Based on these results, species concepts of ectomycorrhizal mushrooms are discussed in detail.

scholarly journals Endangered Uyghur Medicinal PlantFerulaIdentification through the Second Internal Transcribed Spacer

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Congzhao Fan ◽  
Xiaojin Li ◽  
Jun Zhu ◽  
Jingyuan Song ◽  
Hui Yao
Keyword(s):  
Medicinal Plant ◽  
Related Species ◽  
Internal Transcribed Spacer ◽  
Morphological Characteristics ◽  
Its2 Sequence ◽  
Sequence Length ◽  
Local Alignment ◽  
Accurate Identification ◽  
Distance Method ◽  
Closely Related Species

The medicinal plantFerulahas been widely used in Asian medicine, especially in Uyghur medicine in Xinjiang, China. Given that various substitutes and closely related species have similar morphological characteristics,Ferulais difficult to distinguish based on morphology alone, thereby causing confusion and threatening the safe use ofFerula. In this study, internal transcribed spacer 2 (ITS2) sequences were analyzed and assessed for the accurate identification of two salableFerulaspecies (Ferula sinkiangensisandFerula fukangensis) and eight substitutes or closely related species. Results showed that the sequence length of ITS2 ranged from 451 bp to 45 bp, whereas guanine and cytosine contents (GC) were from 53.6% to 56.2%. A total of 77 variation sites were detected, including 63 base mutations and 14 insertion/deletion mutations. The ITS2 sequence correctly identified 100% of the samples at the species level using the basic local alignment search tool 1 and nearest-distance method. Furthermore, neighbor-joining tree successfully identified the genuine plantsF. sinkiangensisandF. fukangensisfrom their succedaneum and closely related species. These results indicated that ITS2 sequence could be used as a valuable barcode to distinguish Uyghur medicineFerulafrom counterfeits and closely related species. This study may broaden DNA barcoding application in the Uyghur medicinal plant field.

Pimpinella ibradiensis (Apiaceae), an unusual new species from Turkey

Phytotaxa ◽  
2015 ◽  
Vol 217 (2) ◽  
pp. 164 ◽  
Author(s):  
İlker Çinbilgel ◽  
özkan Eren ◽  
Hayri Duman ◽  
Mustafa Gökceoğlu
Keyword(s):  
New Species ◽  
Geographical Distribution ◽  
Related Species ◽  
Conservation Status ◽  
Identification Key ◽  
The Other ◽  
Site Conditions ◽  
Closely Related Species

Pimpinella ibradiensis, an unusual new species found in the Toka Yayla (İbradı, Antalya) in southern Anatolia, is described and illustrated. Site conditions, synecology and conservation status of P. ibradiensis are considered. In light of the comparison with the other closely related four species, namely P. nephrophylla, P. flabellifolia, P. sintenisii and P. paucidentata, its similarity within the genus are discussed. P. ibradiensis is easly distinguished from its relatives by its white petals, presence of bracts and bracteoles, larger fruits (4–5.5 × 1–2 mm), and having serrulate basal leaves with 60–95 strongly cartilaginous teeth along margins. The geographical distribution of P. ibradiensis and closely related species are mapped and the identification key of those species is updated.

Fritillaria arsusiana (Lilieae, Liliaceae), a new species from southern Anatolia

Phytotaxa ◽  
2021 ◽  
Vol 502 (2) ◽  
pp. 149-159
Author(s):  
HASAN YILDIRIM ◽  
MEHTAP TEKŞEN
Keyword(s):  
New Species ◽  
Geographical Distribution ◽  
Related Species ◽  
Conservation Status ◽  
Flower Colour ◽  
Identification Key ◽  
Critically Endangered ◽  
Diagnostic Characters ◽  
A New Species ◽  
Shape And Size

In this study, Fritillaria arsusiana (Liliaceae) is described and illustrated as a new species from Hatay province in southern Anatolia, Turkey. It is related to F. amana and F. hermonis by habitus features and broadly campanulate flowers, but differs mainly by its bulb shape and size, smaller leaf and flower features, and flower colour. It has also been morphologically compared with F. wendelboi, F. pinardii, and F. latakiensis. The detailed description, diagnostic characters, original photographs, geographical distribution, habitat and phenology, etymology, conservation status, and identification key of the new and the related species are presented in this study. IUCN conservation status of F. arsusiana is suggested as Critically Endangered (CR).

Trogia benghalensis (Marasmiaceae, Basidiomycota), a new species from India

Phytotaxa ◽  
2017 ◽  
Vol 331 (2) ◽  
pp. 273 ◽  
Author(s):  
ARUN KUMAR DUTTA ◽  
SUDESHNA NANDI ◽  
ENTAJ TARAFDER ◽  
RIMPA SIKDER ◽  
ANIRBAN ROY ◽  
...  
Keyword(s):  
New Species ◽  
Related Species ◽  
Internal Transcribed Spacer ◽  
West Bengal ◽  
Internal Transcribed Spacer Region ◽  
Comprehensive Description ◽  
Closely Related Species ◽  
Molecular Sequence ◽  
Region Data ◽  
A New Species

A new species of Trogia (Marasmiaceae, Basidiomycota) is described from West Bengal, India. Analysis of the molecular sequence (nuclear ribosomal internal transcribed spacer region) data suggests that T. benghalesis is phylogenetically distinct from its closely related species. Comprehensive description based on macro- and microscopic characters, photographs and comparisons with morphologically similar and phylogenetically related species are provided.

Group I Intron Versus its Sequences in Phylogeny of Cetrarioid Lichens

1999 ◽  
Vol 31 (5) ◽  
pp. 441-449 ◽  
Author(s):  
Arne Thell
Keyword(s):  
Ribosomal Rna ◽  
Internal Transcribed Spacer ◽  
Phylogenetic Trees ◽  
Group I Intron ◽  
Ribosomal Genes ◽  
Its Sequences ◽  
Its Sequence ◽  
Ribosomal Rna Gene ◽  
18S Ribosomal Rna Gene ◽  
Group I

AbstractPhylogenetic trees based on group I intron sequences and on internal transcribed spacer (ITS) sequences of mycobiont ribosomal genes were calculated and compared. Eight cetrarioid and four non-cetrarioid species of the Parmeliaceae were compared. The phylogeny based on group I intron sequences is partly congruent with the ITS sequence phylogeny. Group I intron sequences are presumably less informative for infragenic studies. The introns have a length of 214–233 nucleotides, and differ at up to 33% of the bases between species. All introns analysed are located between the positions 1516 and 1517 of the fungal 18S ribosomal RNA gene. Cetrarioid lichens form a non-homogeneous group within the Parmeliaceae according to both group I intron and ITS sequences.

scholarly journals Application of internal transcribed spacer (ITS) sequences for identifying Anoectochilus setaceus Blume in Thanh Hoa, Vietnam

2020 ◽  
Vol 181 (2) ◽  
pp. 108-116
Author(s):  
B. B. Thinh ◽  
L. D. Chac ◽  
L. T.M. Thu
Keyword(s):  
Internal Transcribed Spacer ◽  
Molecular Taxonomy ◽  
Gene Segment ◽  
Dna Barcode ◽  
Its Sequences ◽  
Gene Region ◽  
Its Gene ◽  
Ctab Method ◽  
Young Leaves ◽  
Total Dna

Background. The term “DNA barcode” is used extensively in molecular taxonomy. Basically, this technique is based on the use of a DNA sequence (about 400–800 bp) as a standard to identify and determine the species relation of organisms quickly and accurately. Therefore, DNA barcodes not only help taxonomists in classifying and identifying species, but also improve their ability to control, understand and utilize biodiversity. In this study, the authors conducted identification of samples of Anoectochilus setaceus Blume collected in Thanh Hoa through the isolated sequence of ITS gene regions.Materials and methods. Total DNA was extracted from young leaves of A. setaceus samples using CTAB method. The ITS gene segment was amplified by PCR and sequenced. This genetic sequence was analyzed, compared and used to establish a phylogenetic tree using BioEdit, BLAST and DNASTAR programs.Results and conclusion. We isolated 4 sequences of the ITS gene region in 4 A. setaceus samples collected at Xuan Lien and Pu Luong of Thanh Hoa province; the ITS gene region was 667 nucleotide long. The findings identified the samples as the same species and showed 99% similarity to the ITS gene sequence of A. roxburghii (Wall.) Lindl. published in GenBank, GQ328774. This study also demonstrates that the method employing internal transcribed spacer (ITS) sequences is an effective tool to identify A. setaceus taxa.

scholarly journals Heterorhabditis noenieputensis n. sp. (Rhabditida: Heterorhabditidae), a new entomopathogenic nematode from South Africa

2012 ◽  
Vol 88 (2) ◽  
pp. 139-151 ◽  
Author(s):  
A.P. Malan ◽  
R. Knoetze ◽  
L. Tiedt
Keyword(s):  
South Africa ◽  
New Species ◽  
Internal Transcribed Spacer ◽  
Morphological Traits ◽  
Sequence Data ◽  
Entomopathogenic Nematode ◽  
The Body ◽  
Species Comparison ◽  
Citrus Orchard ◽  
A New Species

AbstractA new entomopathogenic nematode in the genus Heterorhabditis is described from South Africa, from two singular isolates found 1000 km from each other, from beneath a fig tree and in a citrus orchard, respectively. Morphological and molecular studies indicate both isolates to be the same and a new undescribed Heterorhabditis species. Comparison of sequences of the internal transcribed spacer (ITS) rDNA and the D2D3 region of the 28S rDNA gene with available sequences of other described species within the genus, indicate the two isolates as a new species. Phylogenetic analysis of the sequence data concerned placed the new species, H. noenieputensis n. sp., closest to H. indica and H. gerrardi in the indica-group. The new species, H. noenieputensis n. sp., is distinguished from other species in the genus by a combination of several morphological traits of the males and the infective juveniles (IJs). The new species differs from all other species previously described, as regards the body length of the IJs, except for H. indica and H. taysearae, in which the IJ is smaller. The IJ also differs from that of H. indica in the length of the oesophagus, the body diameter, the length of the tail and the E%. In addition, males of H. noenieputensis n. sp. differ from their closest relative, H. indica, in the position of the excretory pore, SW% and D%; and from H. gerrardi in the length of the oesophagus and SW%. The seventh pair of genital papillae of H. noenieputensis n. sp. are normally developed, while for H. indica they are often branched or swollen at the base, while 8 and 9 are usually absent in both species.

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