Pengasingan Strain Baru Kulat dari Tanah Di Malaysia untuk Penghasilan Asid γ –Linolenik (GLA)

2012 ◽  
Author(s):  
A. A. Hamid ◽  
W. M. W. Yusof ◽  
R. M. Illias ◽  
K. Nadarajah
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Fatty Acid Profiles ◽  
Forest Reserves ◽  
Evening Primrose ◽  
Oleaginous Fungi ◽  
Lipid Contents ◽  
Total Fatty Acids ◽  
The Difference

Kajian telah dijalankan untuk mendapatkan strain baru kulat yang menghasilkan GLA hasil penyaringan 21 pencilan yang diperoleh daripada tanah hutan simpan di sekitar Selangor dan Negeri Sembilan. Sebanyak 5 pencilan kulat daripada 21 pencilan yang diperoleh telah dikenalpasti menghasilkan asid γ–linolenik (GLA) (18:3, n-6) iaitu SK8, SK22, 2A1, DR dan K3, yang menghasilkan 6%, 7.6%, 6.9% 5.4% dan 13.5% GLA daripada asid lemak total masing-masing. Kelima-lima pencilan tersebut juga didapati menghasilkan peratusan lipid yang berbeza, iaitu 30%, 20%, 32%, 30% dan 8% lipid total (w/w, biojisim), masing-masing. Pencilan K3 mempunyai kandungan GLA yang setara dengan kandungan GLA dalam minyak ‘Evening Primrose’ yang dihasilkan secara komersial pada masa kini, iaitu 8-12% GLA dari asid lemak total. Pencilan 2A1 menunjukkan penghasilan GLA tertinggi (0.0096g GLA/g glukosa) berbanding SKA, SK22, DR dan K3, yang menghasilkan 0.0032. 0.0039, 0.007 dan 0.0025g, GLA/g glukosa, masing-masing. Perbezaan profil asid lemak bagi keseluruhan 21 pencilan juga dibincangkan. Kata kunci: Oleaginous; kulat; γ-linolenic; asid lemak poli-tidak-tepu. In our studies, 21 filamentous fungi were isolated from soil obtained from various sites of forest reserves in Selangor and Negeri Sembilan. Five isolates (SK8, SK22, 2A1, DR and K3) were found to produced γ–linolenic acid (GLA) (18:3, n–6) at 6%, 7.6%, 6.9%, 5.4% and 13.5% of their respective total fatty acid with lipid contents of 30%, 20%, 32%, 30% and 8% (w/w, biomass), respectively. The GLA content of K3 is comparable to that of the commercially available ‘Evening Primrose’ with GLA comprising of 8–12% of its total fatty acids. The difference in the fatty acid profiles from all 21 isolates is discussed here. Key words: Oleaginous; fungi γ-linolenic; polyunsaturated fatty acids.

scholarly journals Fatty acid profiles of muscle, liver, heart and kidney of Australian prime lambs fed different polyunsaturated fatty acids enriched pellets in a feedlot system

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Hung Van Le ◽  
Don Viet Nguyen ◽  
Quang Vu Nguyen ◽  
Bunmi Sherifat Malau-Aduli ◽  
Peter David Nichols ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Fatty Acid Profiles

Long-chain n-3 polyunsaturated fatty acids contained in liver and muscle tissues of Okinawan long-spine porcupinefish (Diodon holocanthus Linnaeus 1758)

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Yutaka Tashiro
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Ryukyu Islands ◽  
Okinawa Island ◽  
Content Type ◽  
Muscle Tissues ◽  
The Ryukyu Islands ◽  
Total Fatty Acids ◽  
Long Chain

Purpose This study aimed to analyze the lipid content and fatty acid composition in the liver and muscle of a porcupinefish species inhabiting waters around the Ryukyu Islands to investigate their potential as a source of long-chain n-3 polyunsaturated fatty acids (LC-PUFAs). Design/methodology/approach Porcupinefish were collected along the Okinawa Island coast. The composition of fatty acids and cholesterol in both liver and muscle were analyzed using a gas chromatograph mass spectrometer. Findings The liver of Okinawan long-spine porcupinefish was rich in lipids whose content correlated to the proportion of liver/body weight. Fatty acid compositions in their liver and muscles were similar to each other. LC-PUFAs occupied 44% of total fatty acids, with docosahexaenoic acid (DHA) being the dominant (42%), whereas eicosapentaenoic acid occupied 2.4%. The liver contained 1,690 mg of cholesterol and 14.8 g of DHA per 100 g, whose proportion decreased in summer compared to other seasons (p = 0.036). Originality/value The liver of Okinawan long-spine porcupinefish, which has not yet been commercially used although its non-toxicity is claimed, can be an excellent source of LC-PUFAs, especially DHA, accentuating its potential in food supplements’ production.

scholarly journals Effect of feeding tuna oil or soyabean oil as supplements to sows in late pregnancy on piglet tissue composition and viability

1998 ◽  
Vol 80 (3) ◽  
pp. 273-280 ◽  
Author(s):  
J. A. Rooke ◽  
I. M. Bland ◽  
S. A. Edwards
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Late Pregnancy ◽  
Tissue Composition ◽  
Tuna Oil ◽  
Total Fatty Acids ◽  
Effect Of Feeding ◽  
Long Chain ◽  
Viability Score

To investigate whether long-chain n-3 polyunsaturated fatty acids could cross the porcine placenta in late pregnancy and alter neonatal piglet tissue composition, multiparous sows (seven per diet) were fed on diets containing a supplement (30 g/kg) of either soyabean oil or tuna oil for the last 21 d of pregnancy and the first 7 d of lactation. The proportions of all fatty acids, except 18:1n-7, differed between diets: in particular, the tuna-oil-containing diet supplied more 22:6n-3 and less 18:2n-6 fatty acids than the soyabean-oil-containing diet. The proportions of n-3 fatty acids, particularly 22:6n-3 (g/100 g total fatty acids) in sow plasma, colostrum and milk were increased and the proportion of 18:2n-6 was decreased by feeding tuna oil. Piglet tissue n-3 fatty acid proportions (particularly 22:6n-3), obtained shortly after birth, were increased in piglets born to tuna-oil-fed sows compared with progeny of soyabean-oil-fed sows. The increase in the proportion of n-3 fatty acids (g/100 g total fatty acids) in piglet tissues as a result of tuna-oil feeding, compared with soyabean-oil-feeding, was in the order plasma>liver>erythrocytes>spleen>brain>retina. Piglets born to tuna-oil-fed sows had a lower viability score at birth than the progeny of soyabean-oil-fed sows. The proportions of long-chain n-3 fatty acid in tissues of new-born piglets were increased by feeding tuna oil to the sow in late pregnancy; however no improvements in piglet viability were observed.

scholarly journals Nutritional Enhancement of Health Beneficial Omega-3 Long-Chain Polyunsaturated Fatty Acids in the Muscle, Liver, Kidney, and Heart of Tattykeel Australian White MARGRA Lambs Fed Pellets Fortified with Omega-3 Oil in a Feedlot System

Biology ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 912
Author(s):  
Shedrach Benjamin Pewan ◽  
John Roger Otto ◽  
Robert Tumwesigye Kinobe ◽  
Oyelola Abdulwasiu Adegboye ◽  
Aduli Enoch Othniel Malau-Aduli
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Human Health ◽  
Chain Polyunsaturated Fatty Acid ◽  
Fatty Acid Profiles ◽  
Omega 3 ◽  
Pufa Composition ◽  
Nutritional Enhancement ◽  
Long Chain

The aim of this research was to evaluate the nutritional enhancement of omega-3 long-chain polyunsaturated fatty acid (n-3 LC-PUFA) composition of edible lamb Longissimus thoracis et lumborum muscle, heart, kidney, and liver in response to dietary supplementation of lot-fed lambs with or without omega-3 oil fortified pellets. The hypothesis tested was that fortifying feedlot pellets with omega-3 oil will enhance the human health beneficial n-3 LC-PUFA composition of edible lamb muscle tissue and organs. Seventy-five Tattykeel Australian White lambs exclusive to the MARGRA brand, with an average body weight of 30 kg at six months of age, were randomly assigned to the following three dietary treatments of 25 lambs each, and lot-fed as a cohort for 47 days in a completely randomized experimental design: (1) Control grain pellets without oil plus hay; (2) Omega-3 oil fortified grain pellets plus hay; and (3) Commercial whole grain pellets plus hay. All lambs had ad libitum access to the basal hay diet and water. Post-slaughter fatty acid composition of the Longissimus thoracis et lumborum muscle, liver, kidney, and heart were determined using thee gas chromatography–mass spectrophotometry technique. Results indicated significant variations (p < 0.05) in fatty acid profiles between tissues and organs. Omega-3 oil fortified pellets significantly (p < 0.05) increased ≥C20 n-3 LC-PUFA (C20:5n-3 eicosapentaenoate, EPA + C22:5n3 docosapentaenoate, DPA + C22:6n3 docosahexanoate DHA); C18:3n-3 alpha-linolenate, ALA; C18:2 conjugated linoleic acid, CLA; total monounsaturated fatty acids, MUFA; polyunsaturated fatty acids, PUFA contents; and reduced the ratio of omega-6 to omega-3 fatty acids in all lamb organs and tissues without impacting shelf-life. The findings demonstrate that the inclusion of omega-3 oil in feedlot diets of lambs enhances the human health beneficial omega-3 long-chain polyunsaturated fatty acid profiles of edible muscle tissue and organs without compromising meat quality.

Mammary gland and milk fatty acid composition of two dairy goat breeds under feed-restriction

2017 ◽  
Vol 84 (3) ◽  
pp. 264-271 ◽  
Author(s):  
Mariana Palma ◽  
Susana P. Alves ◽  
Lorenzo E. Hernández-Castellano ◽  
Juan Capote ◽  
Noemí Castro ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Mammary Gland ◽  
Fatty Acid ◽  
Dairy Goat ◽  
Feed Restriction ◽  
Fatty Acid Profiles ◽  
Total Fatty Acids ◽  
The Tropics ◽  
Different Levels ◽  
Acid Percentage

Goat dairy products are an important source of animal protein in the tropics. During the dry season, pasture scarcity leads animals to lose up to 40% of their body weight, a condition known as Seasonal Weight Loss (SWL) that is one of the major constraints in ruminant production. Breeds with high tolerance to SWL are relevant to understand the physiological responses to pasture scarcity so they could be used in programs for animal breeding. In the Canary Islands there are two dairy goat breeds with different levels of tolerance to SWL: the Palmera, susceptible to SWL; and the Majorera, tolerant to SWL. Fat is one of the milk components most affected by environmental and physiological conditions. This study hypothesises that feed-restriction affects Majorera and Palmera breeds differently, leading to different fatty acid profiles in the mammary gland and milk. An interaction between breed and feed-restriction was observed in the mammary gland. Feed-restriction was associated with an increase in oleic acid and a decrease in palmitic acid percentage in the Palmera breed whereas no differences were observed in the Majorera breed. Palmitic and oleic acids together constituted around 60% of the total fatty acids identified, which suggests that Palmera breed is more susceptible to SWL. In milk, feed-restriction affected both breeds similarly. Regarding the interaction of the breed with the treatment, we also observed similar responses in both breeds, but this influence affects only around 2% of the total fatty acids. In general, Majorera breed is more tolerant to feed-restriction.

Characterization of 9 Newly Bred Peanut Genotypes for Seed Fatty Acid Profiles Suitable for Biodiesel Production

2014 ◽  
Vol 1073-1076 ◽  
pp. 1134-1137
Author(s):  
Qing Yun Zhang ◽  
Xiu Zhen Wang ◽  
Yue Yi Tang ◽  
Qi Wu ◽  
Quan Xi Sun ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Biodiesel Production ◽  
Fatty Acid Profiles ◽  
High Productivity ◽  
Seed Fatty Acid ◽  
High Oleic ◽  
Total Fatty Acids ◽  
Palmitic Acids

Nine newly bred peanut genotypes from our research group were analyzed for their seed fatty acid composition. Oleic, linoleic and palmitic acids were 3 major fatty acids in peanut seed oil, together constituting 89.65%-91.83% of total fatty acids in peanut seeds. Five of the peanut lines/cultivars tested had an oleate to linoleate ratio (O/L) of 19.11-31.37, and may be classified as high-oleic, providing good sources for biodiesel production. Of them, 12L48 is most promising due to its high productivity and saline tolerance.

Effect of double-muscling in Belgian Blue young bulls on the intramuscular fatty acid composition with emphasis on conjugated linoleic acid and polyunsaturated fatty acids

2001 ◽  
Vol 73 (2) ◽  
pp. 253-260 ◽  
Author(s):  
K. Raes ◽  
S. de Smet ◽  
D. Demeyer
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Polyunsaturated Fatty Acids ◽  
Acid Composition ◽  
Acid Content ◽  
Fatty Acid Content ◽  
Total Fatty Acid Content ◽  
Total Fatty Acids

AbstractThe effect of double-muscling (DM) genotype (double-muscling, mh/mh; heterozygous, mh/+; normal, +/+) of Belgian Blue (BB) young bulls on the intramuscular fatty acid composition, in particular conjugated linoleic acid (CLA) and polyunsaturated fatty acids (PUFA) was examined in five different muscles. The relative fatty acid composition showed only minor differences between muscles within genotypes. However, the DM genotype had a large effect on both the intramuscular total fatty acid content and on the relative fatty acid composition. Across muscles, the mh/mh animals had a lower total fatty acid content compared with the +/+animals (907 v: 2656 mg/100 g muscle;P< 0·01) and a higher PUFA proportion in total fatty acids (27·5 v 11·3 g/100 g total fatty acids;P< 0001), resulting in a higher PUFA/saturated fatty acid ratio (0·55 v 0·18;P< 0·01) and a lower n-6/n-3 ratio (5·34 v. 6·17;P< 0·01). The heterozygous genotype was intermediate between the two homozygous genotypes. The relative CLA content was similar in the mh/mh and +/+ genotypes and approximated 0·4 to 0·5 g/100 g total fatty acids. From the data it is further suggested that differences in the metabolism of the n-3 and n-6 fatty acids could exist between DM genotypes.

Biohydrogenation of n-3 polyunsaturated fatty acids in the rumen and their effects on microbial metabolism and plasma fatty acid concentrations in sheep

2005 ◽  
Vol 81 (2) ◽  
pp. 239-248 ◽  
Author(s):  
L. A. Sinclair ◽  
S. L. Cooper ◽  
S. Chikunya ◽  
R. G. Wilkinson ◽  
K. G. Hallett ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Formic Acid ◽  
Fish Oil ◽  
Marine Algae ◽  
Linseed Oil ◽  
Fatty Acid Content ◽  
Microbial Efficiency ◽  
Total Fatty Acids

AbstractSix cannulated wether sheep weighing 57 (s.d. 4·3) kg were used to investigate the susceptibility of unprotected and protected n-3 polyunsaturated fatty acids from different sources to biohydrogenation in the rumen, their uptake into plasma and effects on ruminal metabolism. The sheep were assigned to one of six dietary treatments formulated to have a similar fatty acid content (60 g/kg DM) and containing: linseed oil (LO), linseed oil absorbed into vermiculite (VLO), formic acid-formaldehyde treated whole linseed (FLS), fish oil (FO), fat encapsulated fish oil (PFO) or a mixture of fish oil and marine algae (1: 1 on an oil basis; AF), in six periods of 28 days duration in a Latin-square design. Biohydrogenation of C20:5(n-3) and C22:6(n-3) was high in FO at approximately 870 g/kg, but reduced to 625 and 625 g/kg respectively for PFO, and 769 and 601 g/kg respectively for AF. Ruminal biohydrogenation of C18:3(n-3) was similar across treatments based on linseed, averaging 860 g/kg, but C18:2(n-6) was lower (P< 0·05) in animals given VLO or FLS at 792 and 837 g/kg respectively, compared with LO (907 g/kg). Duodenal flow of C18:1trans in animals given any of the diets containing fish oil averaged 8·4 g/day compared with 2·8 g/day in animals given diets based on linseed (P< 0·001), whilst cis-9, trans-11 conjugated linoleic acid was not significantly different among treatments. Plasma C20:5(n-3) and C22:6(n-3) proportions were highest in animals given the AF diet (11·8 and 8·2 g per 100 g of the total fatty acids respectively) and lowest in animals given LO (2·8 and 2·7 g per 100 g of the total fatty acids respectively;P< 0·001). By contrast, plasma C18:3(n-3) proportions were highest in animals given the LO or VLO diets at approximately 6·9 g per 100 g of the total fatty acids, and lowest in the AF treatment at 0·9 g per 100 g (P< 0·001). Duodenal non-ammonia-N flow was similar among treatments at 21·0 g/day except in animals given FLS which had the highest flow (25·9 g N per day;P< 0·01). Microbial N flow was also similar among treatments whilst microbial efficiency (g N per kg OM truly degraded in the rumen) was higher (P< 0·05) in animals given FLS than LO, FO or AF. By contrast, ruminal fibre digestion was higher (P< 0·05) in animals given LO or FO than those offered VLO, FLS, PFO or AF. In conclusion, compared with linseed oil, absorption of linseed oil into vermiculite improved duodenal flow but not plasma levels of C18:3(n-3), whilst formic acid-formaldehyde treatment of linseed had little effect on protecting C18:3(n-3) in the rumen, although duodenal non-ammonia nitrogen flow and microbial efficiency were improved. Compared with fish oil, the provision of marine algae or fat encapsulated fish oil resulted in a lower biohydrogenation of C22:6(n-3) and C20:5(n-3), and an increased duodenal flow and plasma concentration and offers the potential to favourably manipulate the n-3 fatty acid composition of sheep meat.

scholarly journals The fatty acid profiles in M. longissimus dorsi and M. semimembranosus of short-haired and long-haired crossbred sheep meat

2018 ◽  
Vol 192 ◽  
pp. 03051
Author(s):  
Pattraphorn Patthararangsarith ◽  
Kunya Tuntivisoottikul
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Chemical Composition ◽  
Longissimus Dorsi ◽  
Fatty Acid Profiles ◽  
Nutritional Values ◽  
Sheep Meat ◽  
Interaction Factor ◽  
Total Fatty Acids ◽  
Brachiaria Mutica

This study was conducted to investigate the effects of crossbred groups and anatomical muscle positions on the chemical composition and fatty acid profiles in the loin (M. longissimus dorsi) and chump (M. semimembranosus) muscles of the short-haired and long-haired crossbred sheep. Five sheep per group with the 25 kg initial body weight were fed with 2 kg/d TMR and free choice grass (Brachiaria mutica), and those were raised 84 days. After slaughter, loin and chump were used to examine the chemical composition and fatty acid profiles. The results showed that the loin from the short-haired and long-haired crossbreds had Palmitic acid at the highest level among the other saturated fatty acid (SFA), whereas this fatty acid was not detected in the chump. However, the highest SFA in the chump of both crossbreds was the Stearic acid. Furthermore, the fatty acids in both groups were detected in the chump more than in the loin. There was found that the nutritional values were not affected by sheep crossbred groups (P>0.05). The loin and chump had affected the percentage of crude protein (P<0.01), the amount of Lauric Acid (C12:0), and the total fatty acids (P<0.01). Interaction factor between the crossbred groups and the anatomical muscle positions was unaffected all the traits studied (P>0.05).

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