scholarly journals The relationship between freezing point of milk and milk components and its changes during lactation in Czech Pied and Holstein cows

Author(s):  
Gustav Chládek ◽  
Vladimír Čejna

The freezing point of milk (FPM) is an instant indicator of violated technological quality of raw milk, especially of dilution. FPM can also vary due to numerous effects associated with changes in milk composition and milk characteristics. Beside the effect of season, phase of lactation, breed, milk yield, sub-clinical mastitis etc. the impacts of nutrition and dietary or metabolic disorders are the most significant and the most frequent (GAJDŮŠEK, 2003). FPM is a relatively stable physical characteristic and due to osmotically active elements it ranges from – 0.510 to – 0.535 °C (HANUŠ et al., 2003b). Recently ŠUSTOVÁ (2001) studied the freezing point of milk in pool samples; she observed seasonal changes in FPM of mixed milk and the effect of different diets on FPM values. KOLOŠTA (2003) looked into the effect of grazing season on FPM. HANUŠ et al. (2003a) analysed possible effects of handling of milk components on FPM.The aim of this work was to describe the relationship between FPM and milk components and the impact of breed, number and phase of lactation on FPM. We analysed 328 milk samples in total, out of which 137 samples were of Czech Pied cows and 191 samples of Holstein cows. The effect of number and phase of lactation was evaluated for both breeds together. The greatest coefficients of correlation in total were found between FPM and lactose content (r = 0.600) and solids non fat (r = 0.523). Lower coefficients of correlation were found between FPM and milk fat content (r = 0.235), milk protein content (r = 0.260) and urea concentration (r = 0.256). These coefficients were considerably lower in Holstein cows than in Czech Pied cows. The coefficients of correlation between FPM and number and phase of lactation and somatic cells count were insignificant. The total mean value of FPM was – 0.534 °C. Breed statistically significantly (P<0.01) affected FPM (+0.006 °C in C breed) and milk fat content (+0.19 % in H breed). Breed highly significantly (P<0.001) affected daily milk yield (+4.9 kg milk in H), milk protein content (+0.27 % in C) and solids non fat (+0.37 % in C). On the contrary, breed had no significant effect on lactose content, urea concentration and somatic cells count.Variability of FPM was greater in Czech Pied cows (5.9 %) than in Holstein cows (0.9 %). Number of lactation had no significant effect on FPM (maximum difference between lactations was 0.008 °C). Phase of lactation had no significant effect on FPM either. Our study revealed the fact that FPM was most of all affected by lactose content and solids non fat. However, the decrease of lactose content was compensated by a tendency of mammary glad to keep constant osmotic pressure. As the somatic sells count was low, there was no decline in lactose content during later lactations so no significant decrease of FPM occurred.

Author(s):  
Martin Skýpala ◽  
Gustav Chládek

Milk yield varies during lactation, following what is termed a lactation curve. ŽIŽLAVSKÝ and MIKŠÍK (1988) recorded changes in milk yield within a day, too. TEPLÝ et al. (1979) a KOUŘIMSKÁ et al. (2007) published variation within a day ± 1.10 kg in milk yield, ± 0.75 % in milk fat content and ± 0.20 % in milk protein content. Milk yield of cows can be expressed in many different ways, for instance, in kilograms per lactation or in kilograms per day. A practical parameter describing milk production is milk yield (kg) per milking.The object of experiment were 12 cows of Holstein cattle on the first lactation from the 100-day of lactation to 200-day of lactation. The samples of milk were collected from January to May 2007, once a month from the morning and evening milking (milking interval 12 h ± 15 min.). The following parameters were monitored: milk production – milk yield (kg), milk protein production (kg), milk fat production (kg); milk composition – milk protein content (%), milk fat content (%), lactose content (%), milk solids-not-fat content (%), milk total solids content (%); technological properties of milk – ti­tra­tab­le acidity (SH), active acidity (pH), rennet coagulation time (s), quality of curd (class) and somatic cell count as a parameter of udder health.Highly significant differences were found (P < 0.01) between morning milk yield (15.7 kg) and evening milk yield (13.8 kg), between morning milk protein production (0.51 kg) and evening milk protein production (0.45 kg) and between evening milk fat content (4.41 %) and morning milk fat content (3.95 %). A significant difference (P < 0.05) was found between morning milk total solids content (12.62 %) and evening milk total solids content (12.07 %). No significant differences were found between morning (M) and evening (E) values of the remaining parameters: milk fat production (M 0.62 kg; E 0.60 kg), milk protein content (M 3.24 %; E 3.27 %), milk lactose content (M 4.78 %; E 4.86 %), milk solids-not-fat content (M 7.69 %; E 7.71 %), somatic cell count (M 80 000/1 mL; E 101 000/1 mL), titratable aci­di­ty (M 7.75 SH; E 7.64 SH), active acidity (M pH 6.58; E pH 6.61), rennet coagulation time (M 189 s.; E 191 s.), quality of curd (M 1.60 class; E 1.57 class).


Author(s):  
A Gavelis ◽  
V. Þilaitis ◽  
A Juozaitis ◽  
V. Juozaitienë ◽  
G. Urbonavièius ◽  
...  

The aim of this study was to evaluate relationship between milk progesterone concentration (P4) and milk traits at the start of estrus time and 12h after start of the estrus in dairy cows. The 96 milk samples of 48 Lithuanian dairy cows without reproduction disorders and 90–100 days after calving were evaluated. Cows were classified into two groups based on milk yield per day: less than 30 kg (n=20) and e”30 kg (n=28). Data were categorized by milk fat and protein content at the start estrus and 12h after start of estrus to evaluate relationship between P4 and milk traits examined. P4 at estrus time in dairy cows was significantly positively correlated with milk yield (P less than 0.001), whereas it was negatively correlated with milk protein (P less than 0.05-P less than 0.01) and fat at 12h after start of estrus. Dairy cows with F/P from 1.0 to 1.5 had the lowest P4 in milk. Results of the pregnancy in dairy cows were related with lower P4 and milk yield level (P less than 0.001), higher milk fat (P less than 0.05) and milk protein content (P less than 0.001). These cows had 1.90 times lower prevalence of the signs of subclinical ketosis (P less than 0.05) at estrus time when compared with non-pregnant cows. As a result, it was clearly demonstrated that P4 in dairy cows can help to evaluate and improve the reproductive properties of cows.


1998 ◽  
Vol 1998 ◽  
pp. 201-201
Author(s):  
R. H. Phipps ◽  
A. K. Jones ◽  
J.G. Perrot

An earlier study conducted at the Centre for Dairy Research (Phipps et al. 1996) using a total mixed ration (TMR) based on maize silage, showed that when distillers grains wheat and molassed sugar beet feed (MSBF) replaced cereal and protein supplements, milk yield and dry matter (DM) intake were maintained but there was a large and significant reduction in milk fat and a small but significant reduction in milk protein. This depression in milk protein was attributed to the high oil content of distillers grains, which would have reduced fermentable metabolisable energy intake which in turn may have adversely affected microbial protein synthesis and reduced milk protein content. The aim of the current study was to devise a nutritional regime using distillers grains maize (DGM) and MSBF in a TMR which would allow milk fat content to be substantially reduced without compromising either DM intake, milk yield or milk protein content.


Nativa ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. 145
Author(s):  
Maura Frare Menegon ◽  
Claudineli Cassia Bueno da Rosa ◽  
Carmen Wobeto ◽  
Marcia Rodrigues Carvalho Oliveira ◽  
Henrique Melo da Silva ◽  
...  

Objetivou-se avaliar o método de espectroscopia por ultrassom (EU) e o efeito do congelamento do leite nas análises de gordura e proteína do leite de vaca. Cem amostras foram analisadas imediatamente após a coleta em tanque de expansão (in natura), congeladas a -20ºC por 30 dias e novamente analisadas por métodos oficiais (gordura por butirômetro de Gerber e proteína por micro-Kjeldahl) e por EU. Observou-se efeito de interação (P < 0,01) entre o método analítico e congelamento do leite. A EU superestimou (P < 0,01) o teor de gordura e subestimou (P < 0,01) o teor de proteína no leite in natura ou congelado. O congelamento não afetou (P = 0,15) o teor de gordura obtido pelo método oficial, mas reduziu o teor de gordura (P < 0,01) obtido com a EU. O congelamento aumentou (P < 0,01) o teor de proteína obtido pelo método oficial, mas reduziu (P < 0,01) quando obtido por EU. Os teores de gordura e proteína do leite obtidos pelo método de EU são divergentes daqueles obtidos por métodos oficiais, independente da amostra ser in natura ou congelada.Palavras-chave: método analítico; análise química; correlação. EFFICACY OF ULTRASONIC SPECTROSCOPY AND FREEZING IN EVALUATION OF MILK CHEMICAL COMPOSITION ABSTRACT: The objective was to evaluate the ultrasound spectroscopy (US) method and the effect of milk freezing on the cow's milk fat and protein analysis. One hundred samples were collected from one milk tank, immediately analyzed, and frozen at -20ºC for 30 days and analyzed again by official method (fat: Gerber butyrometer, protein: micro-Kjeldahl) and US. It was observed effect of interaction (P < 0.01) between analytical method and milk freezing. Ultrasound spectroscopy overestimated milk fat content and underestimated milk protein content on in natura or frozen. The milk freezing for 30 days did not affect the milk fat content obtained by official method (P = 0.15), but it reduced the milk fat content (P < 0.01) obtained by US. The milk freezing increased (P < 0.01) the milk protein content obtained by official method, but reduced (P <0.01) when obtained from US. The milk fat and protein content obtained by US are different from those obtained by official methods, regardless of the sample is in natura or frozen.Keywords: analytical method; chemical analysis; correlation.


Author(s):  
T. V. Pidpala ◽  
Yu. S. Matashnyuk

Under the conditions of intensive technologies used in production of livestock products, the main selection feature that characterizes the economic feasibility of dairy farming and breeding value of animals is milk productivity. One of the factors that affects, not only the economy of production, but also the improvement of herds and breeds of cattle, is the use of highly productive cows. Therefore, the aim of our research was to assess the level of development of productive traits in Holstein cows under the conditions of intensive milk production technology, according to selection and genetic parameters. To conduct the study, a group of 1089 first-born cows was formed with the help of the Dairy Comp program and Microsoft Excel. The material for the research was the milk productivity of Holstein cows during the first three lactations. The level of development of selection traits in animals was determined by selection and genetic parameters. It was found that from the sample n = 1089 to the group of highly productive animals (“>10560”) were included 266 cows, and low-productive animals (“<8706”) – 249 first-borns. They had an average milk yield for the first lactation, 11439 kg of milk with a fat content of 3.96 % and 7737 kg of milk and 3.95 %, respectively. There is a difference in milk yield, milk fat and protein between the groups of cows “>10614” and “<8706”, but there is a general tendency towards changes in productivity with age. Based on the data of milk recurrence and the amount of milk fat of high-yielding cows (group “>10614”), it was found that higher values of the coefficient are characteristic of lactations I–III (rw= 0.105; rw= 0.135). As a result of comparative analysis it was found that low-yielding animals (group “<8706”) were characterized by higher recurrence rates for I–II and I–III lactation (rw = 0.345;rw = 0.316;rw = 0.320 and rw = 0.664;rw = 0.646;rw = 0.651, respectively). Higher rates of recurrence of traits of milk productivity are the characteristic of low-yielding cows (group “<8706”), i.e. they had more consistency of traits during different lactations and animals with a high level of productivity did not differ in age constancy. At a high level of milk yield in cows of group “>10614” appeared a negative correlation of low and medium level (r = -0.423). It was also found a negative correlation of low and medium level between milk yield and protein content in milk (r = -0.007… -0.332). At lower milk yields, there is no negative correlation between milk yield and fat content in milk. Thus, the existence of a negative correlation between milk yield and fat content in milk at a high level of animal productivity, and between milk yield and protein content in milk at both high and low levels of cow productivity was proved.


1998 ◽  
Vol 1998 ◽  
pp. 201-201
Author(s):  
R. H. Phipps ◽  
A. K. Jones ◽  
J.G. Perrot

An earlier study conducted at the Centre for Dairy Research (Phipps et al. 1996) using a total mixed ration (TMR) based on maize silage, showed that when distillers grains wheat and molassed sugar beet feed (MSBF) replaced cereal and protein supplements, milk yield and dry matter (DM) intake were maintained but there was a large and significant reduction in milk fat and a small but significant reduction in milk protein. This depression in milk protein was attributed to the high oil content of distillers grains, which would have reduced fermentable metabolisable energy intake which in turn may have adversely affected microbial protein synthesis and reduced milk protein content. The aim of the current study was to devise a nutritional regime using distillers grains maize (DGM) and MSBF in a TMR which would allow milk fat content to be substantially reduced without compromising either DM intake, milk yield or milk protein content.


Author(s):  
М.Ю. САННИКОВ ◽  
С.И. НОВОПАШИНА ◽  
С.А. ХАТАТАЕВ ◽  
Е.И. КИЗИЛОВА

Изучена молочная продуктивность потомства I, II и III поколений при скрещивании молочных коз в типе зааненской породы с козлами нубийской породы. Установлено, что самый высокий удой за лактацию (576,5±11,62) был у чистопородных зааненских коз (n=72) — на 10,8—25,3% (P≦0,05) больше, чем у помесных животных за счет более длительной лактации (на 13,0%). Среднесуточный удой за лактацию был на 2,1% выше у помесей I поколения (n=26), чем у зааненских, на 4,9% — чем у помесей II (n=24) и на 13,5% — чем у помесей III поколений (n=21) (P≦0,05). Козоматки III поколения по содержанию жира превосходили сверстниц на 0,14—0,50 абс. процента, а белка — на 0,18—0,37 абс. процента. Среднесуточное количество молочного жира было выше у помесей I поколения на 0,9—7,3%, молочного белка — на 1,5—5,1%. За счет более продолжительного периода лактации козоматки зааненской породы превосходили своих помесных сверстниц по количеству молочного жира на 5,3—11,8% (1,2—2,5 кг) и по количеству молочного белка — на 7,7—11,3% (1,2—1,7 кг). Таким образом, поглотительное скрещивание является эффективным приемом для получения чистопородных животных нубийской породы. И может быть использовано в качестве селекционного приема в товарных хозяйствах для повышения качественных показателей молока коз в типе зааненской породы. Milk productivity of the offspring of generations I, II, and III during milk goat breeding in the type of the Saanen breed with goats of the Nubian breed was studied. It was found that the highest milk yield per lactation (576.5±11.62) was demonstrated by purebred Saanen goats (n=72) — by 10.8—25.3% (P≦0.05) more than in crossbred animals due to prolonged lactation (by 13.0%). Average daily milk yield per lactation was 2.1% higher in crossbred animals of generation I (n=26) compared to the Saanen breed, 4.9% higher than in crossbred animals of generation II (n=24), and 13.5% higher than in crossbred animals of generation III (n-21) (P≦0.05). By fat content the breeding goats of generation III are superior to their age-mates by 0.14—0.50 absolute percent, and by protein — by 0.18—0.37 absolute percent. Average daily milk fat content was 0.9—7.3% higher in crossbred animals of generation I; average daily milk protein content was 1.5—5.1% higher. Due to prolonged lactation breeding goats of the Saanen breed were superior to their crossbred age-mates in milk fat quantity by 5.3—11.8% (1.2—2.5 kg), and in milk protein quantity — by 7.7—11.3% (1.2—1.7 kg). Accordingly, accumulation cross breeding is an effective method of obtaining purebred animals of the Nubian breed. It can be used as a selection technique in commercial farming to increase qualitative characteristics of goat milk in the type of the Saanen breed.


2000 ◽  
Vol 25 ◽  
pp. 175-177 ◽  
Author(s):  
J.K. Margerison ◽  
C.J.C. Phillips

AbstractSuckling following mechanical milking is common practice in organic dairy production systems and in developing countries. The objective of the experimental work was to assess the effect of suckling and suckling frequency following mechanical milking on milk yield, milk composition and somatic cell count. Two experiments were completed using multiparous dairy cows allocated at 3 days post partum to their respective treatment groups. In experiment one, twenty–four multiparous dairy cows were allocated to one of two treatments for 305 days; 12 cows not suckled (NS) and 12 cows, which were suckled twice daily following mechanical milking (S2). Daily milk yield was significantly greater (P<0.05) in suckled cows, NS 8.0, S2 8.9 (sem 0.18) kg/d. Milk fat content was significantly lower (P<0.05) in suckled cows (NS 32.0, S2 30.7 (sem 0.56) g/kg). However, milk protein was not significantly different in suckled cows, NS 29.2, S2 27.6 (sem 10.79) g/kg compared with non suckled cows. Somatic cell count was significantly lower (P<0.05) in suckled cows NS 106, S2 85 (sem 2.85) 000/ml, compared with non suckled cows. In experiment two, thirty-eight cows were allocated one of two treatments for 120 days; 19 cows not suckled (NS) and 19 cows, which were suckled once daily following the afternoon milking (S1). Suckling took place for fifteen minutes daily following machine milking only. The calves were weaned at 6 months of age. Total daily milk yield was significantly greater (P<0.05) in suckled cows, NS 11.7, S1 12.5 (sem 0.04) (kg/d) compared with non suckled cows. The milk fat and protein content were not significantly different in suckled and non suckled cows. Milk fat content NS 33.4, S1 32.9 (sem 0.14) g/kg and milk protein content NS 29.8, S1 30.0 (sem 0.07) g/kg. In conclusion, suckled cows had significantly higher milk yields. Cows suckled twice daily had significantly lower milk fat content. Suckling did not affect milk protein content. Suckling cows twice daily significantly reduced SCC.


1999 ◽  
Vol 66 (4) ◽  
pp. 475-487 ◽  
Author(s):  
ZYGMUNT M. KOWALSKI ◽  
PAWEŁ M. PISULEWSKI ◽  
MAURO SPANGHERO

The objective of this study was to determine the effects of supplementing the diets of dairy cows with Ca soaps of rapeseed fatty acids (CSRFA) and rumen-protected (RP) methionine on their milk yield and composition, including milk protein fractions and fatty acids. Twelve Polish Red Lowland cows were used in a complete balanced two period changeover experiment. The four treatment diets were a control consisting of a total mixed ration of grass silage and concentrates, and the total mixed ration supplemented with RP methionine, CSRFA or RP methionine plus CSRFA. Dry matter intake was not affected by diet. Milk yield increased when cows were given the diet with CSRFA, but supplementation of diets with RP methionine did not affect milk yield. Milk protein content, but not milk protein yield, decreased when CSRFA was given. The addition of RP methionine to the control diet and the CSRFA diet produced similar increases in the milk protein content. Supplementation of the diet with CSRFA significantly changed the milk fatty acid profile: the proportions of 10:0, 12:0, 14:0, 15:0 and 16:0 in milk fat decreased, but those of 18:0 and cis-18:1 increased. We conclude that CSRFA can be used in practical dairy diets to increase milk yield and manipulate its fatty acid composition.


1999 ◽  
Vol 66 (2) ◽  
pp. 177-191 ◽  
Author(s):  
RAMON CASALS ◽  
GERARDO CAJA ◽  
XAVIER SUCH ◽  
CELINA TORRE ◽  
SERGIO CALSAMIGLIA

Forty-eight Manchega dairy ewes were used during a complete lactation in a 2×2 factorial design to determine the effects of supplementing diets with fat (calcium soaps of palm oil fatty acids, CSFA) and rumen undegradable protein (RUP) on milk production and composition. Factors tested were amounts of CSFA (0 or 200 g/kg) and RUP (300 or 450 g/kg crude protein) in the concentrate. RUP was altered by adding a mixture of maize gluten meal and blood meal. Lactation was divided into one nursing period (period 1, weeks 1–4), and three milking periods (periods 2–4, weeks 5–8, 9–14 and 15–21). Concentrates were given at 0–8 kg/d during periods 1 and 2, and at 0–6 kg/d in periods 3 and 4. Ewes grazed rotationally in an Italian rye-grass pasture and received a daily supplement of 0·8 kg vetch–oat hay during period 1, and 0·3 kg lucerne hay during periods 2–4. For the whole lactation, supplemental fat markedly increased milk fat content (+23%) and yield (+16%), and decreased milk protein content (−9%). The positive effect of feeding CSFA on milk fat content was more evident at the beginning of lactation; however, its negative effect on milk protein was more pronounced in late lactation. Supplementary RUP had little effect, increasing milk protein content only in period 3, when the crude protein content of pasture was lower. Milk yield and lamb growth were not affected by dietary treatments. The results indicated that CSFA can be useful for increasing the milk fat content of dairy ewes at pasture, which may help farmers to produce milk reaching the minimum requirements of fat content for the cheese industry.


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