Near-Infrared Reflectance Spectroscopy (NIRS) detection to differentiate morning and afternoon milk based on nutrient contents and fatty acid profiles

Milking time is one of the factors that affect milk quality. The objective of this study was to differentiate morning milk from afternoon based on milk fatty acid profile and create a prediction model using Near-Infrared Reflectance Spectroscopy (NIRS). This study used explorative research and post-observation analysis. Milk sampling was collected from three different dairy farm locations in West Java Provinces (Pangalengan district of Bandung Regency, Cibungbulang District of Bogor Regency, and Tanah Sareal District of Bogor Municipality). Milk quality observed in this study included milk fat, protein, lactose, solid non-fat (SNF), and fatty acid compositions. Milk fat, protein, lactose, and SNF were analyzed using Lactoscan. Fatty acid compositions were identified using gas chromatography (GC). Sample spectrums were collected using NIRSflex 500. The difference between morning and afternoon milking was tested using a t-test carried out by SPSS ver. 25. Qualitative calibration of milk quality was conducted using NIRSCal v5.6 by applying the cluster (CLU) method. The results from lactoscan and GC showed that milk fat, caprylic acid, and myristoleic acid, and total SFA were significantly different (Sig. (2-tailed) < 0.05) in morning and afternoon milk. However, NIRS failed to generate a sophisticated model for the milk quality differentiation, which shows a low Q-value (0.0011231). The quantitative analysis accurately produced milk fat and total SFA predictions but failed to accurately predict caprylic acid and myristoleic acid. This study concluded that morning milk could be differentiated from afternoon milk based on milk fat, caprylic acid, myristoleic acid, and total SFA content. The NIRS technology can differentiate between morning and afternoon milk based on quantitative calibration of total fat and SFA.

Effect of milking time, lactation stage and udder traits on milk yield of Red Sokoto and Boer goats in semi-arid, Nigeria

The aim of the study was to evaluate the effect of milking time, lactation stage and udder traits on milk yield of Red Sokoto and Boer goats at the farm of Centre for Dry-land Agriculture, Bayero University Kano, Nigeria. The first evaluation of milk yield was done at early stage of lactation starting after day seven of kidding to exclude colostrum. Daily collection of milk samples followed in the morning and evening until 10 weeks of lactation. The weeks were divided into 3 stages of lactation as early, mid and late lactation stages. Milk samples were measured using a 100 mL measuring cylinder and later converted into grammes. The external udder measurements were taken in cm after seven days post kidding and fortnightly for the period of lactation. The result obtained shows significant difference (p<0.05) in the total morning milk yield between the goats. The total morning milk yield was higher (2642.00 ± 3.18 g) in the Boer goats than in the Red Sokoto (2371.50± 3.18 g). However, the average daily milk yield, total milk yield and total evening milk yield were not significantly (p>0.05) affected by the breeds of goat. Mean milk yield was significantly (p<0.05) influenced by stage of lactation and time of milking. The milk yield was significantly higher (98.91 g) in the late lactation while the least was obtained in the mid stage of lactation (65.44 g). Moreover, the morning milk yield was significantly higher than the evening milk yield. A significant interaction effect (p<0.05) of lactation stage and milking time was similarly observed. The results indicated that breed significantly (p<0.05) influenced most of the udder traits except UC, UW and TFD. Significantly (p<0.05) higher values of UL, UW, TC, RTL, LTL, CH and TFD were recorded in the Red Sokoto. Furthermore, the Boer goat breed had significantly higher values of NT, UC and DBT. Le but de l'étude était d'évaluer l'effet de la traite de traite, de la phase de lactation et des traits de la piste sur le rendement du lait de Red Sokoto et des chèvres de Boer à la ferme du Centre d'agriculture à sec, de l'Université Bayero Kano, du Nigéria. La première évaluation du rendement en lait a été effectuée à un stade précoce de l'allaitement à partir de sept jours de blague pour exclure le colostrum. La collecte quotidienne d'échantillons de lait a suivi le matin et le soir jusqu'à 10 semaines de lactation. Les semaines ont été divisées en 3 étapes de la lactation comme des étapes de début et de lactation tardive. Les échantillons de lait ont été mesurés à l'aide d'un cylindre de mesure de 100 ml et convertis ultérieurement en grammes. Les mensurations externes de la mamelle ont été prises en cm après sept jours après la mise bas et tous les quinze jours pendant la période de lactation. Le résultat obtenu présente une différence significative (p <0,05) dans le rendement le lait total du matin entre les chèvres. Le rendement au lait total du matin était plus élevé (2642,00 ± 3,18 g) dans les chèvres Boer que dans le Sokoto rouge (2371.50 ± 3,18 g). Cependant, le rendement quotidien moyen quotidien, le rendement total du lait et le rendement au lait total de la soirée n'étaient pas significativement (p> 0,05) affectés par les races de chèvre. Le rendement en lait moyen était significativement (p <0,05) influencé par la phase de lactation et du temps de traite. Le rendement en lait était significativement plus élevé (98,91 g) à la fin de la lactation, tandis que le moindre a été obtenu au stade moyen de la lactation (65,44 g). De plus, le rendement au lait du matin était nettement supérieur au rendement le lait du soir. Un effet d'interaction significatif (P <0,05) de stade de lactation et de délai de traite a été observé de la même manière. Les résultats ont indiqué que la race de manière significative (p <0,05) a influencé la plupart des traits de pis de la piste, à l'exception des UC, UW et TFD. De manière significative (p <0,05) des valeurs plus élevées d'UL, UW, TC, RTL, LTL, CH et TFD ont été enregistrées dans le Red Sokoto. De plus, la race de chèvre Boer avait des valeurs significativement plus élevées de NT, UC et DBT.

Evaluation of Scenarios for Improving the Collection System for a Milk Factory in Ethiopia

The milk for a factory in Sululta (Ethiopia) is currently collected at ambient temperature. To increase milk production, the sourcing must be extended. This requires the collection of not only the morning milk but also the evening milk from smallholder farms. To accomplish this, the collection of milk from small farmers has to be improved, whereby the milk quality has to be assured with reasonable cost and environmental impact. A model predicting milk rejection was developed based on initial contamination and time and temperature profiles. With this model, different cooling scenarios we reevaluated regarding the expected effectiveness of reducing the rejection rate during collection. Second, cost estimations were made to implement the scenarios to collect morning and evening milk from smallholder farms. A third criterion was greenhouse gas (GHG) emissions per litre of collected milk. Finally, the feasibility of the scenarios was assessed in terms of technical, practical, and economic aspects. Including both quality and economics, the best scenario can be expected from a cooling centre where farmers bring their milk twice a day, except there are signals that the farmers would not be willing to deliver the evening milk to the centre at night. In that case, an additional collecting system would be needed to increase the milk supply. This would result in higher collection costs and an increased risk of milk rejection at the factory gate. Furthermore, this would reduce the value of the chilling centre, as in that case it would be better to deliver the milk directly to the factory. Both scenarios would increase GHG emissions compared with the current situation. Only the use of an off-grid solar power-driven cooling system at the farms would reduce the GHG emissions. However, this solution is less feasible economically. The applied combination of a simple model, economic analysis and the effect on GHG emissions gives valuable information on the effectiveness and limitations of different cooling scenarios for the milk factory. It can help to successfully apply a scenario for increasing the milk supply.

INFLUENCE OF THE TYPE OF NERVOUS ACTIVITY ON THE MILK PRODUCTION OF COWS

The aim of the research was to evaluate the influence of the type of the nervous system on the productive and reproductive qualities of animals. The type of nervous system (strong, weak) of cows of the Ukrainian red dairy breed was determined by the index of the nervous system, which is based on the variability of the fat content in the morning milk yield after changing the conditions of cows keeping. Under the new conditions of keeping cows with a strong type of nervous activity, the coefficients of variability of the morning milk yield and the fat content in milk practically remained at the same level (their slight increase was noted at the level of 4.5 and 3.1%). In cows of a weak type under similar conditions of keeping, a significant increase in the variability of milk yield (by 53.6%) and fat content in milk (by 266.7%) was found. In animals of both types, in the first days of summer-camp keeping, in comparison with winter-stall keeping, there was an increase in milk yield and a decrease in fat content in milk. In cows of the strong type, the fat content decreased by 0.08% and of the weak type by 0.12%. The average index of the nervous system type in animals of the strong type is 1.18, and in cows of the weak type 2.71 (P> 0.999). Before and during milking, the pulse rate in the cows of weak type was higher by 8.0 and 10.8 beats / min. (P> 0.999), compared with the strong type. The animals of strong type of the nervous system, in comparison with the weak type, had a smaller thickness of the concha and skin, a higher pulse and respiratory rate, and a lower rectal body temperature. In cows of a strong type of the nervous system in postnatal ontogenesis, the formation of productive traits took place more rapidly and they better adapted to the conditions of the external environment. This is evidenced by their high coefficient of relative decline in the growth at the age of one (85.0) than in animals with a weak type of nervous system (78.5). The advantage of cows with a strong type of nervous activity over a weak one in milk yield was 348 kg (P>0.95) and in milk fat 8.0 kg. The service period in animals with a strong type of nervous activity was 6.3 days shorter. The animals with a strong type of nervous activity during the first lactation had a higher adaptation index compared with animals of a weak type. The difference for this indicator is 0.49 (or 3.7%). They also had a higher productivity index for the first (3.1%) and second (4.0%) lactation. Thus, the typological features of the nervous activity of cows affect the formation of the most important functions of the body, which can be used in breeding work to improve the herd.

INTERDEPENDENCE AND DISTRIBUTION OF SUBCLINICAL MASTITIS AND INTRA-MAMMARY INFECTION AMONG UDDER QUARTERS IN JERSEY CROSSBRED COWS

The study was aimed to investigate the interdependence and distribution of subclinical mastitis (SCM) and intra-mammary infection (IMI) among udder quarters in Jersey crossbred cows reared in hot-humid environment. Total 366 quarter wise morning milk samples were collected aseptically after performing California mastitis test (CMT) at milking byre itself to evaluate the incidence of infection in experimental cows maintained at cattle yard, ICAR- National Dairy Research Institute, Eastern Regional Station, Kalyani, West Bengal and subjected to microscopic method of somatic cell count to estimate the level of intra-mammary infection. The overall quarter wise incidence rate of IMI and SCM in Jersey crossbred cows was 54.65% and overall arithmetical mean (±SE) of test day SCC (logarithmic) was 5.377 ± 0.039. The percent incidence of SCM in different quarter was 51.14, 59.79, 48.94 and 58.70 in left fore (LF), left hind (LH), right fore (RF) and right hind (RH) quarters respectively. The higher incidence was observed in hind (59.23%) as comparison to fore (50%) quarters while incidence was found to be slightly more distributed in left (55.56%) than right side (53.76%) quarters. Further, the analysis of variance showed that there was no significant effect of quarter on the log 10SCC level. However, the mean Log 10SCC was found higher for hind and right quarters numerically only. The trend of subclinical mastitis incidence obtained in the current study point out that, the rate of incidence of SCM in different quarters was not similar and was higher in hind quarters and therefore, hind and right side quarter required attention during different udder health management programmes.

Effects of Some Environmental Factors on Milking Time Milk Yield in Red Holstein Cows

The purpose of this study is to investigate the environmental factors on the milking time milk yield of Red Holstein cows. For this aim, 172,826 morning milk yield (MMY) and 172,771 evening milk yield (EMY) data collected between 2001 and 2010 from a dairy farm in Aydin Province, Turkey were used. The THI is over 72 from June to August in the region. The effects of calving month, calving year, parity, lactation month and milking month on MMY and EMY were found to be statistically significant. For every month, year and parity, the MMY means were higher than those of the EMY. The peak daily milk yield was determined on the 46th day of lactation (31.7±0.013 kg). For parity, the highest milk yield means were detected for the third parity: 13.69±0.023 kg for MMY and 11.70±0.021 kg for EMY. In conclusion, taking precautions to protect the cows from heat stress especially for the cows calved in summer and keeping the milking interval equal for whole year would help increasing the milk yield.

Detailed fatty acid profile of milk, cheese, ricotta and by products, from cows grazing summer highland pastures

In this research two-dimensional GC was used to analyse, for the first time, the detailed fatty acid (FA) profiles of 11 dairy matrices: raw milk (evening whole, evening partially skimmed, morning whole, and vat milk), cream, fresh cheese, whey, ricotta, scotta, 6- and 12-month-ripened cheeses, obtained across artisanal cheese- and ricotta-making trials carried out during the summer period while cows were on highland pastures. Samples were collected during 7 cheese- and ricotta-making procedures carried out at 2-week intervals from bulk milk to study possible differences in the transfer and modification of FA. Compared with morning milk, evening milk had fewer de novo synthetised FA. The detailed FA profile of partially skimmed milk differed little from that of evening whole milk before skimming, but the cream obtained differed from partially skimmed milk and from fresh cheese in about half the FA, due mainly to higher contents of all de novo FA, and lower contents of n-3 and n-6 FA. Fresh cheese and whey had similar FA profiles. The ricotta manufacturing process affected the partition of FA between ricotta and scotta, the FA profile of the latter differing in terms of groups and individual FA from the former, whereas ricotta and fresh cheese had similar composition of FA. In general, there was an increase in medium-chain saturated FA, and a decrease in many polyunsaturated FA during the first 6 months of ripening, but not during the second 6 months. Two-dimensional GC yielded a very detailed and informative FA profile on all the 11 dairy products and by-products analysed.

Study on feeding effect of dietary protein sources of on blood or milk urea nitrogen in native cows

A feeding trial with 30 Pabna milking cows of 2 to 4 parities dividing equally into 5 groups was conducted to determine the effect of feeding protein from different sources on blood or milk urea nitrogen, and milk yield or protein content in native cows. Considering a group of cows fed a diet of rice straw and concentrate as the control (T0), two out of the rests were fed either with urea-molasses straw (UMS) (T1) or Matikalai (Vigna mungo) hay ( T2) as sources of basal roughage. The rest two groups of cows were fed the control diet replacing percent (%) of feed protein by the amount of urea and molasses fed to UMS group. The amount of urea and molasses was fed daily either in two meals (T3) or fed to cows mixing with other concentrate feed (T4). Feeding a basal diet of UMS, DS or leguminous hay did not affect milk protein (%) and daily milk production Feeding urea and molasses in meals or mix (T3 and T4) did not affect significantly (p>0.05) BSU and MUN contents. It indicates that feeding urea and molasses in two meals in a day either as a single mix of the two or as a mix of the two with concentrates significantly (p>0.05) reduced the concentration of BSU or MUN without having any change in milk protein (%) of the cows. Dry matter (DM) intake was significantly (p<0.05) higher in T1 treatment group followed by T4, T0, T3 and T2, respectively. Similarly, CP intake was significantly (p<0.05) higher in T1 and T2 treatment groups followed by T3 and T4 treatment groups. The values of CP intake were 490, 770, 760, 630 and 580 g/day for treatment groups T0, T1, T2, T3, and T4, respectively. Feedings urea and molasses as meals (T3) significantly (p<0.05) reduced the fat content in milk compared with other treatment groups. Similar to T3, UMS feeding also significantly (p<0.05) reduced fat content in milk compared to Matikalai hay and T4 treatment groups. Milk urea nitrogen (MUN) content in morning milk was lower compared to evening milk. These data showed that feeding urea or protein of organic sources had effect on BSU and MUN contents in the morning milk but had no significant effect on evening milk. The lower BSU or MUN content in milk of the cows fed urea and molasses either in daily meals or as mix with concentrates may be due mainly to a lower CP intake compared to UMS and Matikalai. Therefore, it may be concluded that feeding urea or organic protein had no significant effect on milk protein percent.Bangladesh J. of Livestock Res. 19(1-2): 85-96, Jan-Dec 2012

Chemical and microbial qualities of morning and evening milk collected from selected dairy farms in Chittagong City of Bangladesh

This study was undertaken to evaluate the nutritional and microbiological qualities of raw milk in the Chittagong City. Eighty samples of raw milk of cow were collected in the morning and evening from the surrounding farms of Chittagong Veterinary and Animal Sciences University (CVASU), Chittagong, Bangladesh. The results of compositional analysis evolved that fat, SNF, protein, lactose, specific gravity, freezing point and mineral were (3.94±0.22%), (7.91±0.17%), (3.11±0.08%), (4.32±0.10%), (1.026±0.00), (-0.46±0.007), (0.68±0.01%), respectively. The fat% of evening milk was significantly higher (p‹0.001) than morning milk. Rest of the parameters were not significant. Microbiological enumeration revealed for the counts of total viable bacteria (cfu/ml) and coliform (cfu/ml) were 730500±639 and 280±1.36 in the morning milk and 628625±566 and 326±1.84 in the evening milk, respectively. The results of the current study indicated that the nutritional quality of evening raw milk was higher than in the morning raw milk.Bang. J. Anim. Sci. 2015. 44 (3):171-175

Influence of milk somatic cell content on Parmigiano-Reggiano cheese yield

The aim of this study was to determine the influence of the somatic cell content (SCC) of milk on Parmigiano-Reggiano cheese yield, produced in commercial cheese factories under field conditions. The study was carried out following the production of 56 batches of Parmigiano-Reggiano in 13 commercial cheese factories by processing milk collected from Italian Friesian cattle herds. The vat-milk (V-milk) used for making each cheese batch was obtained by mixing evening milk (partially skimmed following spontaneous separation of fat overnight, natural creaming) and morning milk. The batches of cheese produced were divided into 5 classes according to the SCC value of the evening milk determined prior to natural creaming (class 1, from 0 to 200 000; 2, 201 000–300 000; 3, 301 000–400 000; 4, 401 000–500 000; 5, over 501 000 cells/ml). The cheese yield was calculated as the amount of 24-h cheese, expressed in kilograms, obtained from 100 kg of V-milk (24 h ACY). The values of fat, crude protein, true protein, casein and 24 h ACY of V-milk were negatively correlated with the somatic cell score (SCS) of the evening milk. Conversely, a positive correlation was observed between chloride and SCS. Fat, protein fractions (crude protein, casein and whey proteins), P and titratable acidity of V-milk were positively correlated with its 24 h ACY, while chloride, pH and SCS showed a negative correlation. A significant drop in 24 h ACY was observed in classes 3, 4 and 5, therefore when the SCC of the evening milk exceeded 300 000 cells/ml. Finally a lower recovery of milk fat in cheese was observed as SCC of evening milk increase.