scholarly journals A comparison of histological and chemical analysis in mechanically separated meat

2011 ◽  
Vol 59 (1) ◽  
pp. 145-152 ◽  
Author(s):  
Petr Komrska ◽  
Bohuslava Tremlová ◽  
Pavel Štarha ◽  
Jana Simeonovová ◽  
Zdenka Randulová
Keyword(s):  
Connective Tissue ◽  
Chemical Analysis ◽  
Histological Analysis ◽  
Calcium Content ◽  
Muscle Fibres ◽  
Chemical Examination ◽  
Bone Fragments ◽  
Mechanically Separated Meat ◽  
Similar Good

The aim of this study was evaluation of quality of mechanically separated chicken meat (MSCM) samples obtained by three different separators, by means of a histological (qualitative and quantitative) and chemical examination. Histological examinations used Green Trichrome and Alizarine red staining. The examination was focused on the evaluation of muscle, fat, collagenous connective tissue, bone fragment and calcium content and on the degree of damage to the muscle fibres. Chemical analysis was focused on calcium-, fat- and collagenous connective tissue content. The product obtained by the separator 1 (hard separation) gave similar good results to the separator 3 (soft separation), while separator 2 (hard separation) gave worse results as for being bone fragments and calcium content. As demonstrated, the quality of the product obtained by the separator 1 has improved over the years. The results of the histological analysis were in accordance with the results of chemical analysis. For the quantitative determination of collagen, calcium and bone fragments, image analysis was used. In the present study, good correlation between quantitative histological analysis and chemical analysis was observed (0.673, 0.718 and 0.809, α = 0.01).

VASCULAR CONNECTIVE TISSUE UNDER THE INFLUENCE OF THYROXINE

1961 ◽  
Vol 36 (2) ◽  
pp. 197-211 ◽  
Author(s):  
lb Lorenzen
Keyword(s):  
Connective Tissue ◽  
Vessel Wall ◽  
Aortic Wall ◽  
Calcium Content ◽  
Sodium Sulphate ◽  
Collagen Content ◽  
Histological Changes ◽  
Hexosamine Content ◽  
Healing Processes

ABSTRACT Biochemical and histological changes in the aortic wall of rabbits were demonstrated following injection of epinephrine and l-thyroxine during 2 weeks. The widespread gross and microscopic changes were accompanied by an increase in hexosamine content and uptake of 35S labeled sodium sulphate, and an increased calcium content, whereas the collagen content, assessed by determination of hydroxyproline, was reduced. Comparison with the effect of epinephrine injections alone showed that thyroxine intensified the damaging effect of epinephrine on the vessel wall and induced more pronounced mucopolysaccharide changes in the aortic wall, presumably acting as a link in the healing processes.

Histology of the vertebral artery–dural junction: relevance to posterolateral approaches to the skull base

2019 ◽  
pp. 1-6
Author(s):  
Robert C. Rennert ◽  
Martin P. Powers ◽  
Jeffrey A. Steinberg ◽  
Takanori Fukushima ◽  
John D. Day ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Vertebral Artery ◽  
Skull Base ◽  
Histological Analysis ◽  
Foramen Magnum ◽  
Entry Site ◽  
Microsurgical Technique ◽  
Vessel Injury ◽  
Far Lateral ◽  
Clear Delineation

OBJECTIVEThe far-lateral and extreme-lateral infrajugular transcondylar–transtubercular exposure (ELITE) and extreme-lateral transcondylar transodontoid (ELTO) approaches provide access to lesions of the foramen magnum, inferolateral to mid-clivus, and ventral pons and medulla. A subset of pathologies in this region require manipulation of the vertebral artery (VA)–dural interface. Although a cuff of dura is commonly left on the VA to avoid vessel injury during these approaches, there are varying descriptions of the degree of VA-dural separation that is safely achievable. In this paper the authors provide a detailed histological analysis of the VA-dural junction to guide microsurgical technique for posterolateral skull base approaches.METHODSAn ELITE approach was performed on 6 preserved adult cadaveric specimens. The VA-dural entry site was resected, processed for histological analysis, and qualitatively assessed by a neuropathologist.RESULTSHistological analysis demonstrated a clear delineation between the intima and media of the VA in all specimens. No clear plane was identified between the connective tissue of the dura and the connective tissue of the VA adventitia.CONCLUSIONSThe VA forms a contiguous plane with the connective tissue of the dura at its dural entry site. When performing posterolateral skull base approaches requiring manipulation of the VA-dural interface, maintenance of a dural cuff on the VA is critical to minimize the risk of vascular injury.

scholarly journals Physico Chemical Analysis of Sapota (Manilkara zapota) Coated by Edible Aloe Vera Gel

2015 ◽  
Vol 3 (1) ◽  
pp. 20-25 ◽  
Author(s):  
N. Padmaja ◽  
S. John Don Bosco ◽  
J. Sudhakara Rao
Keyword(s):  
Chemical Analysis ◽  
Aloe Vera ◽  
Ascorbic Acid Content ◽  
Chemical Characteristics ◽  
Manilkara Zapota ◽  
Aloe Vera Gel ◽  
Physico Chemical ◽  
Dramatic Decline ◽  
Physical And Chemical

The physical and chemical characteristics of the fruit have immense significance as they ultimately affect the quality of processed productsprepared from them. Over ripening of Sapota (Manilkara zapota) fruits at the post-harvest stage usually results in dramatic decline in quality.In the present study, physico chemical analysis (which includes Weight loss, Colour, Texture, TSS, pH, TA and Ascorbic acid content) ofedible Aloe vera gel coated Sapota fruits packed in LDPE and stored at 15 ± 2? were studied at regular intervals of 5 days i.e., 0th,5th, 10th,15thand 20th days. The dip treatment of Aloe vera gel coating 1:2, 7 minutes had best retained the physico chemical characteristics than the othertreatments performed and was found to be the most effective treatment in maintaining the fruit quality attributes along with the shelf lifeextension of about 20 days.DOI: http://dx.doi.org/10.3126/ijasbt.v3i1.11703 Int J Appl Sci Biotechnol, Vol. 3(1): 20-25      

Infl uence of crossbreeding and level of feeding on microstructural indicators of lamb

2020 ◽  
pp. 32-40
Author(s):  
I. I. Dmitrik
Keyword(s):  
Muscle Tissue ◽  
Nutritional Value ◽  
Histological Analysis ◽  
Meat Industry ◽  
North Caucasus ◽  
Marbling Score ◽  
Sheep Meat ◽  
Histological Method ◽  
Meat Productivity

When studying indicators that characterize the quality of meat and its nutritional value as part of the development and improvement of existing research methods, histological analysis of the tissues included in it becomes increasingly important. Histological method has been used to study the microstructure of muscle tissue (m. longissimus dorsi) and chemical composition in sheep of diff erent genotypes and reared at diff erent feeding levels. It has been found that the muscle tissue of sheep obtained from crossbreeding of breeds North Caucasus (SC) with Texel (T) has been characterized by a large amount of muscle fi bers by 5,0 and 11,3 % (P < 0,05), the smaller amount by 5,7 and 18,6 % (P < 0,05) in diameter, a higher “marbling” score by 5,2 and 8,3 points, and the lower content of connective tissue by 0,8 and 1,3 abs.% compared to purebreds North Caucasian and from crossbreeding North Caucasian with Poll Dorset breed (PD). Chemical analysis of meat revealed a tendency to increase the content of dry matter and fat by 1,1 and 0,1 abs. % in lamb genotype T×SC and less than the amount by 1,1 and 0,1 abs.% moisture compared to purebreds and PD×SC. Increasing the energy supply of sheep, regardless of breed affi liation, contributes to the formation by 10 % more number of muscle fi bers per unit area and the decrease in their diameter, which increases the “marbling” of lamb and increases its consumer quality. The results have been obtained indicate the feasibility of using morphometric indicators of muscle tissue in the development of criteria for assessing the quality of sheep meat productivity, intended for practical use in agriculture and the meat industry.

scholarly journals Histological analysis of the association between formocresol and endotoxin in the subcutaneous tissue of mice

2008 ◽  
Vol 19 (1) ◽  
pp. 40-45 ◽  
Author(s):  
Ana Teresa Sant'anna ◽  
Luis Carlos Spolidório ◽  
Lizeti Toledo Oliveira Ramalho
Keyword(s):  
Connective Tissue ◽  
Chronic Inflammation ◽  
Histological Analysis ◽  
Subcutaneous Tissue ◽  
Experimental Period ◽  
Connective Tissues ◽  
Plastic Tube ◽  
Tissue Samples ◽  
Subcutaneous Connective Tissue ◽  
Original Formula

This study performed a histological analysis of the effect of formocresol associated to endotoxin (LPS) in the subcutaneous connective tissue of mice. Ninety mice were randomly assigned to 3 groups (n=30). Each animal received one plastic tube implant containing endotoxin solution (10 mg/mL), formocresol (original formula) or a mixture of endotoxin and formocresol. The endotoxin and formocresol groups served as controls. The periods of analysis were 7, 15 and 30 days. At each experimental period, tissue samples were collected and submitted to routine processing for histological analysis. Endotoxin and formocresol produced necrosis and chronic inflammation at 7 and 15 days. At 30 days, the endotoxin group showed no necrosis, while in the formocresol group necrosis persisted. The formocresol-endotoxin association produced necrosis and chronic inflammation in the same way as observed with formocresol at all experimental periods. In conclusion, formocresol seems not to be able to inactive the toxic effects of endotoxin in connective tissues.

V.—Manganese in the Sea

1876 ◽  
Vol 1 (2) ◽  
pp. 50-53 ◽  
Author(s):  
A. H. Church
Keyword(s):  
Chemical Analysis ◽  
Deep Sea ◽  
The Other ◽  
Manganese Nodules ◽  
Red Clay ◽  
Chemical Examination ◽  
Sea Bottom ◽  
Ample Supply

To the geologist, the mineralogist and the chemist, two of the observations made during the voyage of the Challenger are of especial interest. One of these observations is the occurrence over vast areas of the deep sea bottom of a peculiar red clay, containing silica, peroxide of iron, and alumina. The other discovery to which I refer has been described by Sir Wyville Thomson as the occurrence throughout this red clay of nodules of “nearly pure peroxide of manganese.” To these nodules, as well us to the red clay, an organic origin has been assigned. But the immediate source of so much manganese is hard to find, for this element is by no means an abundant constituent of animal or vegetable organisms. The difficulty is, however, somewhat lessened when the manganese nodules are submitted to a more minute chemical examination. From two correspondents I have received an ample supply of these curious concretions, accompanied by a suggestion that they should be submitted to chemical analysis.

scholarly journals Comparative quality of the forms of decoction pieces evaluated by multidimensional chemical analysis and chemometrics: Poria cocos, a pilot study

2019 ◽  
Vol 27 (3) ◽  
pp. 766-777 ◽  
Author(s):  
Li-Xia Zhu ◽  
Jun Xu ◽  
Yun Wu ◽  
Li-Fei Su ◽  
Kelly Yin Ching Lam ◽  
...  
Keyword(s):  
Pilot Study ◽  
Chemical Analysis ◽  
Poria Cocos

scholarly journals HISTOLOGICAL ANALYSIS AND PERFORMANCE OF SOUR PASSION FRUIT POPULATIONS UNDER DIFFERENT ROOTSTOCKS RESISTANT TO Fusarium spp

2018 ◽  
Vol 40 (1) ◽  
Author(s):  
Moisés Ambrósio ◽  
Willian Krause ◽  
Celice Alexandre Silva ◽  
Laís Alves Lage ◽  
Natan Ramos Cavalcante ◽  
...  
Keyword(s):  
Histological Analysis ◽  
Block Design ◽  
Welding Process ◽  
Anatomical Study ◽  
Optical Microscope ◽  
Passion Fruit ◽  
Randomized Block Design ◽  
Physical And Chemical ◽  
Sour Passion Fruit

ABSTRACT The present study aimed to describe histological traits of the graft welding process and evaluate the effect of rootstocks on the physical and chemical traits and the productivity of sour passion fruit populations. The experiment was arranged in a randomized block design, in a 4x2 + 4 factorial scheme (cultivars / canopy x rootstocks), three replicates and nine plants per plot. Commercial cultivars and populations and Passiflora alata and P. nitida rootstocks, in addition to ungrafted plants were used as canopy. Cleft grafting was the grafting type adopted. Traits analyzed such as productivity and physical and chemical quality of fruits were submitted to analysis of variance and compared by the Tukey test. The canopy morphology was evaluated according to descriptors of the Ministry of Agriculture, Livestock and Supply. Grafting was carried out for each graft/rootstock combination for histological analysis. Sections were obtained by freehand cuts, stained and mounted on semi-permanent slides, examined under optical microscope and photomicrographed. Rootstocks affected the early cultivation and reduced productivity and number of fruits of passion fruit populations. Grafting did not affect the quality of fruits or the canopy morphology. Considering the complete culture cycle, P. alata species is able to be used as rootstock. The anatomical study demonstrates the occurrence of better compatibility in the connection of P. edulis tissues on P. alata, compared to grafting on P. nitida.

scholarly journals A STUDY TO ACCESS THE PHYSIO-CHEMICAL PARAMETERS OF WATER QUALITY OF SELECTED PONDS FROM THREE DISTRICTS OF TAMIL NADU, INDIA

2021 ◽  
Vol 21 (2) ◽  
Author(s):  
Kamalasan Prathap- Mathan ◽  
Joseph Babila- Jasmine ◽  
Muthukumar Thilagavathi
Keyword(s):  
Tamil Nadu ◽  
Microbial Contamination ◽  
Renewable Resource ◽  
Chemical Parameters ◽  
Chemical Examination ◽  
Physiochemical Parameters ◽  
The Earth ◽  
Living Organisms ◽  
Physical And Chemical

Water is the most abundant and non-renewable resource in the earth, which play an important role in all living organisms. A study on physiochemical parameters of Manur, Vallanadu and Sundarapandiapatinam ponds from Tirunelveli, Thootukudi and Ramanathapuram districts of Tamil Nadu, India, has addressed the seasonal changes. During the month of January to April samples from Manur was chemically portable, Vallanadu sample was physically portable, Sundarapandiapatinam samples were physically and chemically not portable. During the month of May to August Vallanadu and Manur samples were portable by its physical and chemical examination, Sundarapandiapatinam samples were non portable by its physiochemical property. September to December the samples from all the three ponds were non portable because of exceeding the permissible limit. All the samples were bacteriologically unsafe in nature because of its microbial contamination. This implies the water bodies are not fit for domestic and drinking purpose, thus proper management has to be done by the society and implement government guidelines to save the natural resources from manmade activities.

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