Structural Optimization of Microfluidic Gate Controlled Magnetic Bead System for DNA Sequencing-by-Synthesis

2008 ◽  
Author(s):  
Hesaam Esfandyarpour ◽  
Ronald W. Davis
Keyword(s):  
Structural Optimization ◽  
Dna Sequencing ◽  
Intermediate Layer ◽  
Detection Efficiency ◽  
Magnetic Bead ◽  
Real System ◽  
The Real ◽  
Structure Geometry ◽  
Sequencing By Synthesis ◽  
And Control

In this paper we present a structural and geometrical optimization of a microfluidic gate-controlled magnetic bead system for DNA Sequencing by Synthesis technology. To optimize the efficiency of the technology, we designed, fabricated and tested several structures of the GCMB platform. Different designs of the system were created to study the effects of the pressure, structure, geometry, materials, thickness of intermediate layer between flow and control lines; Toward optimization of the system for a better sensing and to concentrate signal (e.g. heat in Thermosequencing [4–9]) into specific regions for detection in the real system, we recommend an optimized modified gated structure for the microfluidic detection platform and show how this new platform could improve the detection efficiency.

scholarly journals Gate-Controlled Microfluidic Chamber With Magnetic Bead for DNA Sequencing-by-Synthesis Technology

2007 ◽  
Author(s):  
Hesaam Esfandyarpour ◽  
Ronald W. Davis
Keyword(s):  
Dna Sequencing ◽  
Magnetic Beads ◽  
Magnetic Bead ◽  
Synthesis Methods ◽  
Dna Polymerization ◽  
Microfluidic Chamber ◽  
Pdms Channel ◽  
Potential Applications ◽  
Sequencing By Synthesis ◽  
And Control

In this paper we present a novel microfluidic platform for DNA sequencing-by-synthesis methods (e.g. pyrosequencing). The proposed platform is based on the valve-controllable PDMS channel technology with DNA-coated magnetic beads. The encapsulation of the reaction of DNA polymerization in picoliter-sized wells provides for excellent isolation and control for detection. This separation prevents cross-talk amongst neighbor reactors which is one of the most limitations for higher integration of the current technologies. Through application of an external magnetic field the beads can be allocated with better accuracy. In addition this property can help mixing for the reaction. The proposed system is useful for a number of other bio-species detection and sorting templates. This paper illustrates the design and experimental results of a primary template as well as different advantages and potential applications of the Gate-Controlled Magnetic Bead (GCMB) platform in the world of DNA sequencing and genetics.

scholarly journals A Tool for the Analysis and Characterization of School Mathematical Models

Mathematics ◽  
2021 ◽  
Vol 9 (13) ◽  
pp. 1569
Author(s):  
Jesús Montejo-Gámez ◽  
Elvira Fernández-Ahumada ◽  
Natividad Adamuz-Povedano
Keyword(s):  
Mathematical Modeling ◽  
Mathematical Models ◽  
Educational Research ◽  
Analysis Procedure ◽  
Real System ◽  
The Real ◽  
School Model ◽  
Corresponding Analysis ◽  
Three Components

This paper shows a tool for the analysis of written productions that allows for the characterization of the mathematical models that students develop when solving modeling tasks. For this purpose, different conceptualizations of mathematical models in education are discussed, paying special attention to the evidence that characterizes a school model. The discussion leads to the consideration of three components, which constitute the main categories of the proposed tool: the real system to be modeled, its mathematization and the representations used to express both. These categories and the corresponding analysis procedure are explained and illustrated through two working examples, which expose the value of the tool in establishing the foci of analysis when investigating school models, and thus, suggest modeling skills. The connection of this tool with other approaches to educational research on mathematical modeling is also discussed.

scholarly journals Joint structure for the real-time estimation and control of automotive dry clutch engagement

2018 ◽  
Vol 51 (15) ◽  
pp. 1062-1067 ◽  
Author(s):  
Mojtaba Sharifzadeh ◽  
Mario Pisaturo ◽  
Arash Farnam ◽  
Adolfo Senatore
Keyword(s):  
Real Time ◽  
Time Estimation ◽  
The Real ◽  
Joint Structure ◽  
Clutch Engagement ◽  
Real Time Estimation ◽  
Dry Clutch ◽  
Estimation And Control ◽  
And Control

Analysis and Application of BIM Technology in the Project Goal Control

2013 ◽  
Vol 671-674 ◽  
pp. 2978-2981 ◽  
Author(s):  
Ding Tang Wang
Keyword(s):  
Project Management ◽  
Large Data ◽  
The Real ◽  
Virtual Construction ◽  
Potential Problems ◽  
Project Construction ◽  
Visualization Technology ◽  
And Control

BIM is a method which is based on the integration of digitalization technology and visualization technology to manage information related project construction. This paper states the build process of imitating reality by using BIM’s visualization technology with multi-dimension and large data, and unique features for finding the potential problems and risks under virtual construction environment by BIM’s technology, so as to find problems in advance and evaluate them preliminarily, put forward corresponding countermeasures and prevent measures, work out optimized scheme to guide the real construction, and control effectively the project’s quality, progress and cost. BIM solves fundamentally the management defects from traditional project, and will certainly bring about a series of great changes in the circles of project management.

scholarly journals Association study of RS535296987 in TEX14 gene in azoospermiamen: RFLP and DNA sequencing

Genetika ◽  
2021 ◽  
Vol 53 (2) ◽  
pp. 729-737
Author(s):  
Mohammad Vahedi ◽  
Masoud Sheidai
Keyword(s):  
Dna Sequencing ◽  
Dna Sequences ◽  
Chromosome 17 ◽  
Protein Coding ◽  
Nucleotide Variability ◽  
Functional Studies ◽  
Low Degree ◽  
Different Populations ◽  
Human Chromosome 17 ◽  
And Control

Azoospermia is one of the kinds of male infertility, with clinically the most severe phenotype as the natural conception cannot occur. It has been estimated to affect 0.1 to 1% of all men and 10-15% of men in infertile couples. TEX14 (Testis expressed 14, intercellular bridge forming factor) is a protein coding gene, which is located in human chromosome 17, (17q22). Tex14 gene appears to be crucial for perfect spermatogenesis and functional studies indicate the role of TEX14 in the intercellular bridges between developing male germ cells. The gene contains 32 exons and spans 137 kb. A heterogeneousresultis available on the association TEX14 gene and azoospermia. Therefore, it is suggested to investigate this gene in different populations. We analyzed about 200 men in two categories of azoospermia and healthy persons by RFLP as well as DNA sequencing to indicate an association between rs535296987 in TEX14 and its adjacent nucleotides to azoospermia. We found no significant association based on RFLP data and also by clustering of case and control specimens based on DNA sequencing. In general, a low level of nucleotide variability was observed in DNA sequences. Therefore, both eternity in the studied samples and low degree of mutations in this genetic region, may be the reason for heterogeneous reports on association of TEX14 and azoospermia.

scholarly journals Scaling up visual attention and visual working memory to the real world

2020 ◽  
Author(s):  
Timothy F. Brady ◽  
Viola S. Störmer ◽  
Anna Shafer-Skelton ◽  
Jamal Rodgers Williams ◽  
Angus F. Chapman ◽  
...  
Keyword(s):  
Working Memory ◽  
Visual Attention ◽  
Visual Working Memory ◽  
Real World ◽  
Scaling Up ◽  
The Real ◽  
Vision Science ◽  
Precise Understanding ◽  
Complex Settings ◽  
And Control

Both visual attention and visual working memory tend to be studied with very simple stimuli and low-level paradigms, designed to allow us to understand the representations and processes in detail, or with fully realistic stimuli that make such precise understanding difficult but are more representative of the real world. In this chapter we argue for an intermediate approach in which visual attention and visual working memory are studied by scaling up from the simplest settings to more complex settings that capture some aspects of the complexity of the real-world, while still remaining in the realm of well-controlled stimuli and well-understood tasks. We believe this approach, which we have been taking in our labs, will allow a more generalizable set of knowledge about visual attention and visual working memory while maintaining the rigor and control that is typical of vision science and psychophysics studies.

Real-Name Registration Regulation in China

2016 ◽  
pp. 61-87
Author(s):  
Kenneth C. C. Yang ◽  
Yowei Kang
Keyword(s):  
Control Systems ◽  
Political Freedom ◽  
The Internet ◽  
Registration System ◽  
The Real ◽  
Internet Users ◽  
Access Information ◽  
The Government ◽  
And Control

On February 4, 2015, China announced its new regulations that require all Chinese Internet users to register with their real names. The heightened control of Internet clearly demonstrates Chinese government's concerns over increasing social unrests and the abilities of Chinese Internet users to access information not censored by the government. However, the real-name registration regime has posed the greatest challenge to the anonymity of the Internet that many Chinese users have valued in an authoritarian society. Furthermore, the real-name registration system also impinges on Chinese Internet users' privacy, political freedom, and freedom of speech. This book chapter analyzes microblog discussions to examine existing Chinese censorship and control systems on the Internet, to investigate government's rhetoric to justify its censorship and control systems, and to identify major themes in Chinese netizens' reactions and discourses.

scholarly journals Brazilian Plants: An Unexplored Source of Endophytes as Producers of Active Metabolites

Planta Medica ◽  
2019 ◽  
Vol 85 (08) ◽  
pp. 619-636 ◽  
Author(s):  
Daiani Cristina Savi ◽  
Rodrigo Aluizio ◽  
Chirlei Glienke
Keyword(s):  
Species Diversity ◽  
Dna Sequencing ◽  
Species Identification ◽  
Plant Diversity ◽  
Active Metabolites ◽  
The Real ◽  
Host Interaction ◽  
The World

AbstractBrazil has an extraordinary biodiversity, and for many years, has been classified as the first of 17 countries with a mega diversity, with 22% of the total plants in the world (more than 55 000 species). Considering that some endophytes are host-specific, the incomparable plant diversity found in Brazil encompasses an immeasurable variety of habitats and may represent a repository of unexplored species. As a result of the endophyte-host interaction, plant-associated microorganisms have an enormous biosynthetic potential to produce compounds with novelties in structure and bioactivity. Numerous studies have been published over the years describing the endophytic species isolated in Brazil. Identification of these species is generally performed via DNA sequencing. However, many of the genera to which the described taxa belong were reviewed phylogenetically and many species were reclassified. Thus, there is a gap in the real biodiversity of endophytes isolated in Brazil in the last decade. In this scenario, the present study reviewed the biodiversity of endophytes isolated from plants found in different Brazilian biomes from 2012 to 2017, including the following topics: (i) species diversity, (ii) species identification challenges, (iii) biotechnological aspects, and (iv) identified metabolites. Endophytes of 54 species of plants were studied from 2012 to 2017, resulting in the identification of 300 genera, with Diaporthe and Bacillus being the most frequent fungal and bacterial genera, respectively.

scholarly journals A Method For Parallel, Automated, Thermal Cycling of Submicroliter Samples

2001 ◽  
Vol 11 (3) ◽  
pp. 441-447
Author(s):  
Jonathan Nakane ◽  
David Broemeling ◽  
Roger Donaldson ◽  
Andre Marziali ◽  
Thomas D. Willis ◽  
...  
Keyword(s):  
Dna Sequencing ◽  
Thermal Cycling ◽  
High Throughput ◽  
Dna Analysis ◽  
Detection Efficiency ◽  
Large Fraction ◽  
Cycle Sequencing ◽  
High Throughput Dna Sequencing ◽  
Reaction Volumes ◽  
The Cost

A large fraction of the cost of DNA sequencing and other DNA-analysis processes results from the reagent costs incurred during cycle sequencing or PCR. In particular, the high cost of the enzymes and dyes used in these processes often results in thermal cycling costs exceeding $0.50 per sample. In the case of high-throughput DNA sequencing, this is a significant and unnecessary expense. Improved detection efficiency of new sequencing instrumentation allows the reaction volumes for cycle sequencing to be scaled down to one-tenth of presently used volumes, resulting in at least a 10-fold decrease in the cost of this process. However, commercially available thermal cyclers and automated reaction setup devices have inherent design limitations which make handling volumes of <1 μL extremely difficult. In this paper, we describe a method for thermal cycling aimed at reliable, automated cycling of submicroliter reaction volumes.

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