An Ultra Fast Cooling Method for Cell Vitrification Cryopreservation Utilizing Thin Film Evaporation

2016 ◽  
Author(s):  
Fengmin Su ◽  
Nannan Zhao ◽  
Yangbo Deng ◽  
Bohan Tian ◽  
Chunfeng Mu ◽  
...  
Keyword(s):  
Thin Film ◽  
Cooling Rate ◽  
Experimental Results ◽  
Thin Film Evaporation ◽  
Ultra Fast Cooling ◽  
Film Evaporation ◽  
Cooling Method ◽  
A Cell ◽  
Fast Cooling ◽  
Cryoprotective Solution

Ultra-fast cooling is the key to successful cell vitrification cryopreservation of lower concentration cryoprotective solution. This research develops a cell cryopreservation methodology which utilizes thin film evaporation and achieves vitrification of relatively low concentration cryoprotectant with an ultra-fast cooling rate. Experimental results show that the average cooling rate of dimenthylsulphoxide cryoprotective solution reaches 150,000°C/min in a temperature range from 10°C to −180°C. The ultra-fast cooling rate can remarkably improve the vitrification tendencies of the cryoprotective solution. This methodology opens the possibility for more successful cell vitrification cryopreservation.

An Ultrafast Vitrification Method for Cell Cryopreservation

2017 ◽  
Vol 140 (1) ◽  
Author(s):  
Fengmin Su ◽  
Nannan Zhao ◽  
Yangbo Deng ◽  
Hongbin Ma
Keyword(s):  
Thin Film ◽  
Cooling Rate ◽  
Experimental Results ◽  
Low Concentration ◽  
Temperature Range ◽  
Thin Film Evaporation ◽  
Film Evaporation ◽  
A Cell ◽  
Cryoprotective Solution ◽  
Ultrafast Cooling

Ultrafast cooling is the key to successful cell vitrification cryopreservation of lower concentration cryoprotective solution. This research develops a cell cryopreservation methodology which utilizes thin film evaporation and achieves vitrification of relatively low concentration cryoprotectant with an ultrafast cooling rate. Experimental results show that the average cooling rate of dimethylsulfoxide (DMSO) cryoprotective solution reaches 150,000 °C/min in a temperature range from 10 °C to −180 °C. The ultrafast cooling rate can remarkably improve the vitrification tendencies of the cryoprotective solution. This methodology opens the possibility for more successful cell vitrification cryopreservation.

Comparison of 4 Methods for Scalable Kinetic (Fast) Vitrification of Cells: From Theoretical Considerations to Feasibility and Practicality

2013 ◽  
Author(s):  
Igor I. Katkov ◽  
Vladimir F. Bolyukh ◽  
Stephen B. Jones ◽  
Vsevolod Katkov
Keyword(s):  
Thin Film ◽  
Thin Films ◽  
Heat Pipes ◽  
Thin Film Evaporation ◽  
Ultra Fast Cooling ◽  
Film Evaporation ◽  
Fast Cooling ◽  
Rate Of Cooling ◽  
Theoretical Considerations ◽  
Fast Rates

Kinetic (very rapid) vitrification (K-VF) is a promising approach for cryopreservation (CP) of cells but existing methods are not scalable due to the Liedenfrost effect (LFE), which substantially impedes the rate of cooling. Here, we compare 4 emerging approaches that discuss scalability and ultra-fast cooling, namely, cryogenic oscillating heat pipes (COHP), microstructured evaporation in thin films, K-VF in nanodroplets and thin film evaporation in microstructured with our methods of hyperfast cooling KrioBlastTM. We show that only KrioBlastTM can ensure hyper-fast rates of cooling by elimination of the LFE. The logistics and other aspects of practicality of 4 methods are also discussed.

Thin-Film Evaporation Heat Transfer of Liquid Nitrogen and Its Application in Cell Vitrification

2020 ◽  
Vol 142 (7) ◽  
Author(s):  
Fengmin Su ◽  
Yiming Fan ◽  
He Xu ◽  
Nannan Zhao ◽  
Yulong Ji ◽  
...  
Keyword(s):  
Thin Film ◽  
Heat Transfer ◽  
Survival Rate ◽  
Cell Survival ◽  
Cooling Rate ◽  
Liquid Nitrogen ◽  
Film Boiling ◽  
Thin Film Evaporation ◽  
Film Evaporation ◽  
Cell Survival Rate

Abstract Cell vitrification has been an important method of cell cryopreservation. The faster the cooling rate is, the higher the cell survival rate is. However, in conventional cell vitrification methods, film boiling forms a vapor-blanket on the surface, which hinders further improvement of the cooling rate. To eliminate the problem, this article attempted to replace film boiling with thin-film evaporation (TFE) of liquid nitrogen. The experimental system was developed to investigate the TFE heat transfer characteristics of liquid nitrogen. Then, prostate cancer cells were cryopreserved by TFE vitrification method, open pulled straw vitrification method, and equilibrium freezing method. The results showed that the vitrification method of TFE obtained a higher cooling rate and better cell survival rate than the two other cell cryopreservation methods. Thus, the feasibility of this method was preliminarily proved viable when applied to the cell vitrification process. In addition, both the cooling rate and the cell survival rate are affected by the concentration of the cryoprotectant in the cell suspension. The cooling rate decreases as the concentration of the cryoprotectant increases, but the cell survival rate increases first and decrease afterward with an increase in the cryoprotectant concentration, in which an optimum value exists. This study demonstrates the practicality of the new ultrafast cell vitrification method.

scholarly journals Ultra-high cooling rate utilizing thin film evaporation

2012 ◽  
Vol 101 (11) ◽  
pp. 113702 ◽  
Author(s):  
Fengmin Su ◽  
Hongbin Ma ◽  
Xu Han ◽  
Hsiu-hung Chen ◽  
Bohan Tian
Keyword(s):  
Thin Film ◽  
Cooling Rate ◽  
Thin Film Evaporation ◽  
Film Evaporation

Ultra-High Speed Vitrification of Prostate Cancer Cells Based on Thin Film Evaporation

2019 ◽  
Author(s):  
Fengmin Su ◽  
Yiming Fan ◽  
He Xu ◽  
Nannan Zhao ◽  
Yangbo Deng ◽  
...  
Keyword(s):  
Thin Film ◽  
Survival Rate ◽  
Cell Survival ◽  
Cooling Rate ◽  
High Speed ◽  
Thin Film Evaporation ◽  
Film Evaporation ◽  
Freezing Method ◽  
Cell Survival Rate ◽  
Ultra High Speed

Abstract Thin film evaporation is an efficient phase change heat transfer style, and could achieve ultra-high cooling rate if it was applied for cells vitrification. In this paper, an experimental study for prostate cancer cells vitrification was done. The cells ultra-high speed freezing method was based on thin film evaporation of liquid nitrogen. In order to examine the feasibility of the new method, the comparison experiments, in which the other two generic approaches of cell cryopreservation were used, were done. The methods were respectively the equilibrium freezing method and the open pulled straws vitrification method. At the same time, the influences of the concentration of cryoprotectant on cooling rate and cell survival rate were analyzed. The results showed that the ultra-high speed freezing method based on thin film evaporation can obtain higher cooling rate and better cell survival rate than the other two conventional cryopreservation methods. It preliminarily proved the feasibility of this method applied to the cells vitrification process. In addition, both the cooling rate and the cell survival rate are affected by the concentration of the cryoprotectant in the cell suspension. The cooling rate decreases with the concentration of the cryoprotectant increasing, but cell survival rate increases first and decrease afterwards with the increase of the concentration of the cryoprotectant, in which an optimum value exists. This study will promote the practicality of the new ultra-fast cell freezing method.

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