Role of Cells in Freezing-Induced Cell-Fluid Matrix Interactions Within Engineered Tissues

2012 ◽  
Author(s):  
Angela Seawright ◽  
Altug Ozcelikkale ◽  
J. Craig Dutton ◽  
Bumsoo Han
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Regenerative Medicine ◽  
Biological Tissues ◽  
Cellular Viability ◽  
Long Term Storage ◽  
Matrix Interactions ◽  
Term Storage

Cryopreservation can provide long-term storage of various biological tissues, which has significant impact on tissue engineering and regenerative medicine. For successful cryopreservation of tissues, tissue functionality must be maintained including physical properties such as mechanical, optical, and transport properties, as well as cellular viability. Such properties are associated with the extracellular matrix (ECM) microstructure. Thus, the preservation of the ECM microstructure may lead to successful cryopreservation [1,2]. Yet, there is still very little known about changes in the ECM microstructure during freezing/thawing.

Effects of Freezing-Induced Cell-Fluid-Matrix Interactions on Cells and Extracellular Matrix of Engineered Tissues

2011 ◽  
Author(s):  
Ka Yaw Teo ◽  
J. Craig Dutton ◽  
Frederick Grinnell ◽  
Bumsoo Han
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Regenerative Medicine ◽  
Tissue Level ◽  
Tissue Type ◽  
Enabling Technology ◽  
Freeze Thaw ◽  
Engineered Tissues ◽  
Matrix Interactions

Long-term cryopreservation of functional engineered tissues (ETs) is a key enabling technology for tissue engineering and regenerative medicine. However, a limited understanding of tissue-level biophysical phenomena during freeze/thaw (F/T) and their effects on cells and ECM microstructure poses significant challenges for i) preserving tissue functionality, and ii) controlling highly tissue-type dependent cryopreservation outcomes.

Spatiotemporal Deformation of Engineered Tissue During Freezing

2009 ◽  
Author(s):  
Ka Yaw Teo ◽  
J. Craig Dutton ◽  
Bumsoo Han
Keyword(s):  
Extracellular Matrix ◽  
Cellular Viability ◽  
Long Term Storage ◽  
Preservation Technology ◽  
Engineered Tissue ◽  
Frozen State ◽  
Microstructural Integrity ◽  
Long Term Preservation ◽  
Term Storage

A reliable long-term preservation technology is highly desired to provide “off-the-shelf” availability of various engineered tissues (ETs). Among the existing preservation techniques, cryopreservation, which is preserving biomaterials in the frozen state, remains the primary candidate for long-term storage of ETs. One of the most significant challenges in cryopreservation is the lack of consistency in maintaining the functionality of ETs [1]. Since the functionality is closely related to the microstructural integrity of extracellular matrix (ECM) and cellular viability, the ECM microstructure should be maintained as well as the viability of cells [2, 3].

scholarly journals Regulatory redox state in tree seeds

2017 ◽  
Vol 86 (4) ◽  
Author(s):  
Ewelina Ratajczak ◽  
Karl Josef Dietz
Keyword(s):  
Redox State ◽  
Redox Status ◽  
Long Term Storage ◽  
Tree Seeds ◽  
Term Storage ◽  
Intermediate Seeds

Peroxiredoxins (Prx) are important regulators of the redox status of tree seeds during maturation and long-term storage. Thioredoxins (Trx) are redox transmitters and thereby regulate Prx activity. Current research is focused on the association of Trx with Prx in tree seeds differing in the tolerance to desiccation. The results will allow for better understanding the regulation of the redox status in orthodox, recalcitrant, and intermediate seeds. The findings will also elucidate the role of the redox status during the loss of viability of sensitive seeds during drying and long-term storage.

Understanding Actinides through the Role of 5f Electrons

MRS Bulletin ◽  
2001 ◽  
Vol 26 (9) ◽  
pp. 684-688 ◽  
Author(s):  
T. Gouder ◽  
F. Wastin ◽  
J. Rebizant ◽  
G.H. Lander
Keyword(s):  
Predictive Power ◽  
Quality Data ◽  
Nuclear Fuels ◽  
High Quality ◽  
High Quality Data ◽  
Long Term Storage ◽  
5F Electrons ◽  
Term Storage

Studies of the actinide elements and compounds were (and are) motivated by the need to characterize their structural and thermodynamic properties for the development of nuclear fuels and the treatment of waste, whether it be for long-term storage or ideas involving transmutation in high-powered accelerators. For the most part, tables giving these data exist, although the data for transuranium compounds are rather sparse. A much more difficult task is to understand the data and develop theories that have predictive power in this part of the periodic table. In doing this, however, we are confronted with the extremely complicated electronic structure of the 5f shell and the great paucity of high-quality data on materials containing transuranium isotopes.

scholarly journals Integrated Modelling of Runoff, Alkalinity, and pH on a Daily Basis

1985 ◽  
Vol 16 (2) ◽  
pp. 89-104 ◽  
Author(s):  
S. Bergström ◽  
B. Carlsson ◽  
G. Sandberg ◽  
L. Maxe
Keyword(s):  
River System ◽  
Daily Basis ◽  
Integrated Modelling ◽  
Running Waters ◽  
Short Term ◽  
Long Term Storage ◽  
Semi Empirical ◽  
Term Storage

Based on the experience from runoff and groundwater recharge simulation a model system has been developed for terrestrial, hydrochemical, and hydrological simulations. The system emphasizes the role of temporary or long term storage in the aquifers of a basin and, separately, accounts for each rainfall or snowmelt event from its entrance into the ground until mixing in the river system. The model is primarily intended for simulation of natural short term variations in alkalinity and pH in running waters. The hydrochemical processes are modelled in a semi-empirical way without assumption of complete hydrochemichal mass-balance. In the paper a brief hydrochemical background is given, and a model with two alternative hydrochemical sub-structures is described. Examples of daily simulations of runoff alkalinity and pH from three different basins are given.

Adult Neurogenesis in the Songbird: Region-Specific Contributions of New Neurons to Behavioral Plasticity and Stability

2016 ◽  
Vol 87 (3) ◽  
pp. 191-204 ◽  
Author(s):  
Carolyn L. Pytte
Keyword(s):  
Behavioral Plasticity ◽  
Brain Regions ◽  
Long Term Storage ◽  
Behavioral Stability ◽  
New Information ◽  
Neuron Function ◽  
Motor Production ◽  
Term Storage

Our understanding of the role of new neurons in learning and encoding new information has been largely based on studies of new neurons in the mammalian dentate gyrus and olfactory bulb - brain regions that may be specialized for learning. Thus the role of new neurons in regions that serve other functions has yet to be fully explored. The song system provides a model for studying new neuron function in brain regions that contribute differently to song learning, song auditory discrimination, and song motor production. These regions subserve learning as well as long-term storage of previously learned information. This review examines the differences between learning-based and activity-based retention of new neurons and explores the potential contributions of new neurons to behavioral stability in the song motor production pathway.

scholarly journals Crosstalk between Substrates and Rho-Associated Kinase Inhibitors in Cryopreservation of Tissue-Engineered Constructs

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Arindam Bit ◽  
Awanish Kumar ◽  
Abhishek Kumar Singh ◽  
Albert A. Rizvanov ◽  
Andrey P. Kiassov ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Regenerative Medicine ◽  
Kinase Inhibitors ◽  
Cell Attachment ◽  
Human Mesenchymal Stem Cells ◽  
Cellular Viability ◽  
Technical Limitation ◽  
Engineered Tissues

It is documented that human mesenchymal stem cells (hMSCs) can be differentiated into various types of cells to present a tool for tissue engineering and regenerative medicine. Thus, the preservation of stem cells is a crucial factor for their effective long-term storage that further facilitates their continuous supply and transportation for application in regenerative medicine. Cryopreservation is the most important, practicable, and the only established mechanism for long-term preservation of cells, tissues, and organs, and engineered tissues; thus, it is the key step for the improvement of tissue engineering. A significant portion of MSCs loses cellular viability while freeze-thawing, which represents an important technical limitation to achieving sufficient viable cell numbers for maximum efficacy. Several natural and synthetic materials are extensively used as substrates for tissue engineering constructs and cryopreservation because they promote cell attachment and proliferation. Rho-associated kinase (ROCK) inhibitors can improve the physiological function and postthaw viability of cryopreserved MSCs. This review proposes a crosstalk between substrate topology and interaction of cells with ROCK inhibitors. It is shown that incorporation of ionic nanoparticles in the presence of an external electrical field improves the generation of ROCK inhibitors to safeguard cellular viability for the enhanced cryopreservation of engineered tissues.

The Role of Soil Management in Minimizing Water and Nutrient Losses from the Urban Landscape

EDIS ◽  
2013 ◽  
Vol 2013 (11) ◽  
Author(s):  
George Hochmuth ◽  
Laurie Trenholm ◽  
Esen Momol ◽  
Don Rainey ◽  
Claire Lewis ◽  
...  
Keyword(s):  
Urban Areas ◽  
Urban Landscape ◽  
Water Infiltration ◽  
Nutrient Losses ◽  
Long Term Storage ◽  
Important Building Block ◽  
Term Storage ◽  
Reduced Water

Soil is the most important building block of a healthy, attractive landscape, serving many important physical, chemical, and biological functions. Soil provides a physical substrate for plant support and holds nutrients and water for plant use. It also facilitates groundwater recharge (water moving from surface water to groundwater) and provides long-term storage for organic matter. Soil also provides a habitat for microorganisms that aid in the transformation and availability of nutrients. Soil is an integral part of any ecosystem, but urbanization often changes soils in ways that negatively affect plant development. Soils in urban areas may have reduced water infiltration, resulting in increased runoff and increased potential for nutrient losses. Homeowners in urban areas often overcompensate for poor planting conditions by applying inappropriate amounts of fertilizer and water. These practices eventually lead to nutrient losses through stormwater runoff or soil leaching, and these lost nutrients negatively impact groundwater and ecosystems in nearby springs, streams, and water bodies. This 6-page fact sheet was written by George Hochmuth, Laurie Trenholm, Esen Momol, Don Rainey, Claire Lewis, and Brian Niemann, and published by the UF Department of Soil and Water Science, November 2013. http://edis.ifas.ufl.edu/ss593

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