Effects of Freezing-Induced Cell-Fluid-Matrix Interactions on Cells and Extracellular Matrix of Engineered Tissues

2011 ◽  
Author(s):  
Ka Yaw Teo ◽  
J. Craig Dutton ◽  
Frederick Grinnell ◽  
Bumsoo Han
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Regenerative Medicine ◽  
Tissue Level ◽  
Tissue Type ◽  
Enabling Technology ◽  
Freeze Thaw ◽  
Engineered Tissues ◽  
Matrix Interactions

Long-term cryopreservation of functional engineered tissues (ETs) is a key enabling technology for tissue engineering and regenerative medicine. However, a limited understanding of tissue-level biophysical phenomena during freeze/thaw (F/T) and their effects on cells and ECM microstructure poses significant challenges for i) preserving tissue functionality, and ii) controlling highly tissue-type dependent cryopreservation outcomes.

Role of Cells in Freezing-Induced Cell-Fluid Matrix Interactions Within Engineered Tissues

2012 ◽  
Author(s):  
Angela Seawright ◽  
Altug Ozcelikkale ◽  
J. Craig Dutton ◽  
Bumsoo Han
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Regenerative Medicine ◽  
Biological Tissues ◽  
Cellular Viability ◽  
Long Term Storage ◽  
Matrix Interactions ◽  
Term Storage

Cryopreservation can provide long-term storage of various biological tissues, which has significant impact on tissue engineering and regenerative medicine. For successful cryopreservation of tissues, tissue functionality must be maintained including physical properties such as mechanical, optical, and transport properties, as well as cellular viability. Such properties are associated with the extracellular matrix (ECM) microstructure. Thus, the preservation of the ECM microstructure may lead to successful cryopreservation [1,2]. Yet, there is still very little known about changes in the ECM microstructure during freezing/thawing.

Functional Imaging of Matrix Structure of Cryopreserved Engineered Tissues Using Back-Directional Gated Mesoscopic Imaging

2012 ◽  
Author(s):  
Young L. Kim ◽  
Zhengbin Xu ◽  
Altug Ozcelikkale ◽  
Bumsoo Han
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Three Dimensional ◽  
Clinical Use ◽  
Matrix Structure ◽  
Non Invasive ◽  
Engineered Tissues ◽  
Cell Tissue ◽  
Matrix Interactions ◽  
Non Destructive

Successful cryopreservation of engineered tissues (ETs) can greatly advance the access and availability of cell/tissue engineering products for clinical use. One of the key challenges in cryopreserving ETs is that the functionality of ETs should be maintained throughout the preservation process. Many of the functionalities are associated with the extracellular matrix (ECM) microstructure, which in turn can be a crucial marker for the post-thaw functionality. Recent studies also reported that the ECM microstructure can be affected by freezing-induced cell-fluid-matrix interactions.1–3 Thus, it is critical to assess three-dimensional (3-D) matrix structure of cryopreserved ETs in a non-destructive, non-invasive, and rapid manner.

scholarly journals Crosstalk between Substrates and Rho-Associated Kinase Inhibitors in Cryopreservation of Tissue-Engineered Constructs

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Arindam Bit ◽  
Awanish Kumar ◽  
Abhishek Kumar Singh ◽  
Albert A. Rizvanov ◽  
Andrey P. Kiassov ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Regenerative Medicine ◽  
Kinase Inhibitors ◽  
Cell Attachment ◽  
Human Mesenchymal Stem Cells ◽  
Cellular Viability ◽  
Technical Limitation ◽  
Engineered Tissues

It is documented that human mesenchymal stem cells (hMSCs) can be differentiated into various types of cells to present a tool for tissue engineering and regenerative medicine. Thus, the preservation of stem cells is a crucial factor for their effective long-term storage that further facilitates their continuous supply and transportation for application in regenerative medicine. Cryopreservation is the most important, practicable, and the only established mechanism for long-term preservation of cells, tissues, and organs, and engineered tissues; thus, it is the key step for the improvement of tissue engineering. A significant portion of MSCs loses cellular viability while freeze-thawing, which represents an important technical limitation to achieving sufficient viable cell numbers for maximum efficacy. Several natural and synthetic materials are extensively used as substrates for tissue engineering constructs and cryopreservation because they promote cell attachment and proliferation. Rho-associated kinase (ROCK) inhibitors can improve the physiological function and postthaw viability of cryopreserved MSCs. This review proposes a crosstalk between substrate topology and interaction of cells with ROCK inhibitors. It is shown that incorporation of ionic nanoparticles in the presence of an external electrical field improves the generation of ROCK inhibitors to safeguard cellular viability for the enhanced cryopreservation of engineered tissues.

Freezing-Induced Cell-Fluid-Matrix Interaction in Engineered Tissue

2008 ◽  
Author(s):  
Junkyu Jung ◽  
Ka Yaw Teo ◽  
J. Craig Dutton ◽  
Bumsoo Han
Keyword(s):  
Extracellular Matrix ◽  
Biological Tissues ◽  
Tissue Level ◽  
Cellular Level ◽  
Tissue Type ◽  
Freezing And Thawing ◽  
Matrix Interaction ◽  
Engineered Tissues ◽  
Quantitative Understanding ◽  
Engineered Tissue

Freezing of biological tissues occurs in cryomedicine applications such as cryosurgery and cryopreservation. Although cellular level biophysics during freezing and thawing (F/T) has been extensively studied, tissue level biophysics is not fully understood yet. Especially, the effects of F/T on the functionalities of tissue are not well understood so that the outcomes of cryomedicine applications are highly tissue-type dependent [1]. Although many of the functionalities are associated with the extracellular matrix (ECM), the effect of F/T on ECM microstructure has been overlooked. Quantitative understanding on the post-thaw ECM structure is rarely available, but it is essential to design and improve cryopresevation and cryotherapy protocols for a wide variety of native and engineered tissues.

BioMEMS Technologies for Regenerative Medicine

2008 ◽  
Vol 1139 ◽  
Author(s):  
Jeffrey T. Borenstein
Keyword(s):  
Drug Delivery ◽  
Regenerative Medicine ◽  
Drug Delivery Systems ◽  
Ex Vivo ◽  
Delivery Systems ◽  
Organ Shortage ◽  
Engineered Tissues ◽  
Drug Concentrations

AbstractThe emergence of BioMEMS fabrication technologies such as soft lithography, micromolding and assembly of 3D structures, and biodegradable microfluidics, are already making significant contributions to the field of regenerative medicine. Over the past decade, BioMEMS have evolved from early silicon laboratory devices to polymer-based structures and even biodegradable constructs suitable for a range of ex vivo and in vivo applications. These systems are still in the early stages of development, but the long-term potential of the technology promises to enable breakthroughs in health care challenges ranging from the systemic toxicity of drugs to the organ shortage. Ex vivo systems for organ assist applications are emerging for the liver, kidney and lung, and the precision and scalability of BioMEMS fabrication techniques offer the promise of dramatic improvements in device performance and patient outcomes.Ultimately, the greatest benefit from BioMEMS technologies will be realized in applications for implantable devices and systems. Principal advantages include the extreme levels of achievable miniaturization, integration of multiple functions such as delivery, sensing and closed loop control, and the ability of precision microscale and nanoscale features to reproduce the cellular microenvironment to sustain long-term functionality of engineered tissues. Drug delivery systems based on BioMEMS technologies are enabling local, programmable control over drug concentrations and pharmacokinetics for a broad spectrum of conditions and target organs. BioMEMS fabrication methods are also being applied to the development of engineered tissues for applications such as wound healing, microvascular networks and bioartificial organs. Here we review recent progress in BioMEMS-based drug delivery systems, engineered tissue constructs and organ assist devices for a range of ex vivo and in vivo applications in regenerative medicine.

3D bioprinting for meniscus tissue engineering: a review of key components, recent developments and future opportunities

2021 ◽  
Author(s):  
Xavier Barceló ◽  
Stefan Scheurer ◽  
Rajesh Lakshmanan ◽  
Cathal J Moran ◽  
Fiona Freeman ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Meniscal Repair ◽  
Spatial Patterning ◽  
3D Bioprinting ◽  
Research Areas ◽  
Engineered Tissues ◽  
Meniscal Tissue ◽  
Meniscus Tissue ◽  
Recent Developments

3D bioprinting has the potential to transform the field of regenerative medicine as it enables the precise spatial patterning of biomaterials, cells and biomolecules to produce engineered tissues. Although numerous tissue engineering strategies have been developed for meniscal repair, the field has yet to realize an implant capable of completely regenerating the tissue. This paper first summarized existing meniscal repair strategies, highlighting the importance of engineering biomimetic implants for successful meniscal regeneration. Next, we reviewed how developments in 3D (bio)printing are accelerating the engineering of functional meniscal tissues and the development of implants targeting damaged or diseased menisci. Some of the opportunities and challenges associated with use of 3D bioprinting for meniscal tissue engineering are identified. Finally, we discussed key emerging research areas with the capacity to enhance the bioprinting of meniscal grafts.

scholarly journals Nanoengineering in Cardiac Regeneration: Looking Back and Going Forward

Nanomaterials ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1587
Author(s):  
Caterina Cristallini ◽  
Emanuela Vitale ◽  
Claudia Giachino ◽  
Raffaella Rastaldo
Keyword(s):  
Tissue Engineering ◽  
Experimental Study ◽  
Extracellular Matrix ◽  
Regenerative Medicine ◽  
Cardiac Tissue ◽  
Cardiac Regeneration ◽  
Cardiac Cells ◽  
Integration Process ◽  
Specific Interest ◽  
Looking Back

To deliver on the promise of cardiac regeneration, an integration process between an emerging field, nanomedicine, and a more consolidated one, tissue engineering, has begun. Our work aims at summarizing some of the most relevant prevailing cases of nanotechnological approaches applied to tissue engineering with a specific interest in cardiac regenerative medicine, as well as delineating some of the most compelling forthcoming orientations. Specifically, this review starts with a brief statement on the relevant clinical need, and then debates how nanotechnology can be combined with tissue engineering in the scope of mimicking a complex tissue like the myocardium and its natural extracellular matrix (ECM). The interaction of relevant stem, precursor, and differentiated cardiac cells with nanoengineered scaffolds is thoroughly presented. Another correspondingly relevant area of experimental study enclosing both nanotechnology and cardiac regeneration, e.g., nanoparticle applications in cardiac tissue engineering, is also discussed.

scholarly journals Cell-Electrospinning and Its Application for Tissue Engineering

2019 ◽  
Vol 20 (24) ◽  
pp. 6208 ◽  
Author(s):  
Jiyoung Hong ◽  
Miji Yeo ◽  
Gi Hoon Yang ◽  
GeunHyung Kim
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Regenerative Medicine ◽  
Basic Process ◽  
Future Perspectives ◽  
Viable Cells ◽  
Materials Used

Electrospinning has gained great interest in the field of regenerative medicine, due to its fabrication of a native extracellular matrix-mimicking environment. The micro/nanofibers generated through this process provide cell-friendly surroundings which promote cellular activities. Despite these benefits of electrospinning, a process was introduced to overcome the limitations of electrospinning. Cell-electrospinning is based on the basic process of electrospinning for producing viable cells encapsulated in the micro/nanofibers. In this review, the process of cell-electrospinning and the materials used in this process will be discussed. This review will also discuss the applications of cell-electrospun structures in tissue engineering. Finally, the advantages, limitations, and future perspectives will be discussed.

scholarly journals Nanotechnology in the Regeneration of Complex Tissues

2014 ◽  
Vol 5 ◽  
pp. BTRI.S12331 ◽  
Author(s):  
John W. Cassidy
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Extracellular Matrix ◽  
Regenerative Medicine ◽  
Modern Medicine ◽  
The Self ◽  
Mechanical Support ◽  
Related Field ◽  
Biomimetic Scaffolds ◽  
Repair Mechanisms

Modern medicine faces a growing crisis as demand for organ transplantations continues to far outstrip supply. By stimulating the body's own repair mechanisms, regenerative medicine aims to reduce demand for organs, while the closely related field of tissue engineering promises to deliver “of-the-self” organs grown from patients' own stem cells to improve supply. To deliver on these promises, we must have reliable means of generating complex tissues. Thus far, the majority of successful tissue engineering approaches have relied on macroporous scaffolds to provide cells with both mechanical support and differentiative cues. In order to engineer complex tissues, greater attention must be paid to nanoscale cues present in a cell's microenvironment. As the extracellular matrix is capable of driving complexity during development, it must be understood and reproduced in order to recapitulate complexity in engineered tissues. This review will summarize current progress in engineering complex tissue through the integration of nanocomposites and biomimetic scaffolds.

scholarly journals Cell Biological Techniques and Cell-Biomaterial Interactions

Cells ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 2094
Author(s):  
Yunqing Kang
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
The Body ◽  
The Self ◽  
Self Healing ◽  
Special Issue ◽  
Underlying Mechanisms

Biomaterials play a key role in modern tissue engineering and regenerative medicine. They are expected to take over the function of a damaged tissue in the long term, trigger the self-healing potential of the body, and biodegrade at an appropriate rate. To meet these requirements, it is imperative to understand the cell-biomaterial interactions and develop new cell biotechnologies. The collection of this Special Issue brings together a number of studies portraying the underlying mechanisms of cell-biomaterial interactions.

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