Non-Invasive Assessment of Lymphatic Pumping Pressure in a Rat Tail Model Utilizing Near-Infrared Imaging

2013 ◽  
Author(s):  
J. Brandon Dixon ◽  
Ryan Akin ◽  
Mike Weiler ◽  
Timothy Kassis
Keyword(s):  
Near Infrared ◽  
Infrared Imaging ◽  
Immune Cell ◽  
Vessel Diameter ◽  
Lipid Absorption ◽  
Cell Trafficking ◽  
Non Invasive ◽  
Venous Circulation ◽  
Lymphatic Vasculature ◽  
Rat Tail Model

The lymphatic vasculature consists of a network of vessels that promote unidirectional transport of fluid, proteins, and cells from the interstitium back into the blood, providing functions essential for maintaining fluid balance, immune cell trafficking, and lipid absorption from the intestine. The lymphatics generate flow through both extrinsic pumping mechanisms, such as contraction of surrounding skeletal muscle, and through the intrinsic contractility of each lymphatic vessel unit known as a lymphangion. Specialized lymphatic muscle, working in coordination with uni-directional valves separating each lymphangion, serves to contract up to 80% of the vessel diameter and drive flow from the interstitium back to the venous circulation.

Characterization of Near-Infrared Functional Lymphatic Imaging in the Rat Tail Model

2013 ◽  
Author(s):  
Michael Weiler ◽  
J. Brandon Dixon
Keyword(s):  
Near Infrared ◽  
Immune Cell ◽  
Imaging Modality ◽  
Lymphatic Vessels ◽  
The Body ◽  
Cell Trafficking ◽  
Nir Imaging ◽  
Lymphatic Vasculature ◽  
Rat Tail Model ◽  
Lymphatic Imaging

The lymphatic vasculature is present in nearly every tissue of the body to serve essential functions in fluid homeostasis, immune cell trafficking, and lipid transport, and it has been implicated in the progression of several diseases. Despite the critical roles that this system performs, very little is known about the lymphatic vasculature in comparison to the blood vasculature, which can be attributed, in part, to the difficulty associated with imaging lymphatic vessels. With the growing interest in studying lymphatics, near-infrared (NIR) imaging has emerged in the literature as a novel lymphatic imaging modality to simultaneously improve spatial resolution to visualize small initial lymphatics and increase temporal resolution to capture the dynamic lymphatic pump function responsible for fluid propulsion.

scholarly journals Non-invasive monitoring of chronic liver disease via near-infrared and shortwave-infrared imaging of endogenous lipofuscin

2020 ◽  
Vol 4 (8) ◽  
pp. 801-813 ◽  
Author(s):  
Mari Saif ◽  
Wilhelmus J. Kwanten ◽  
Jessica A. Carr ◽  
Ivy X. Chen ◽  
Jessica M. Posada ◽  
...  
Keyword(s):  
Liver Disease ◽  
Chronic Liver Disease ◽  
Near Infrared ◽  
Infrared Imaging ◽  
Chronic Liver ◽  
Invasive Monitoring ◽  
Non Invasive ◽  
Shortwave Infrared

scholarly journals Map4k4 impairs energy metabolism in endothelial cells and promotes insulin resistance in obesity

2017 ◽  
Vol 313 (3) ◽  
pp. E303-E313 ◽  
Author(s):  
Rachel J. Roth Flach ◽  
Marina T. DiStefano ◽  
Laura V. Danai ◽  
Ozlem Senol-Cosar ◽  
Joseph C. Yawe ◽  
...  
Keyword(s):  
Immune Cell ◽  
Vascular Dysfunction ◽  
Chylous Ascites ◽  
Mitogen Activated Protein Kinase ◽  
Cell Trafficking ◽  
Metabolic Dysfunction ◽  
Vascular Integrity ◽  
Adult Mice ◽  
Mitogen Activated Protein ◽  
Lymphatic Vasculature

The blood vasculature responds to insulin, influencing hemodynamic changes in the periphery, which promotes tissue nutrient and oxygen delivery and thus metabolic function. The lymphatic vasculature regulates fluid and lipid homeostasis, and impaired lymphatic function can contribute to atherosclerosis and obesity. Recent studies have suggested a role for endothelial cell (EC) mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4) in developmental angiogenesis and lymphangiogenesis as well as atherosclerosis. Here, we show that inducible EC Map4k4 deletion in adult mice ameliorates metabolic dysfunction in obesity despite the development of chylous ascites and a concomitant striking increase in adipose tissue lymphocyte content. Despite these defects, animals lacking endothelial Map4k4 were protected from skeletal muscle microvascular rarefaction in obesity, and primary ECs lacking Map4k4 displayed reduced senescence and increased metabolic capacity. Thus endothelial Map4k4 has complex and opposing functions in the blood and lymphatic endothelium postdevelopment. Whereas blood endothelial Map4k4 promotes vascular dysfunction and impairs glucose homeostasis in adult animals, lymphatic endothelial Map4k4 is required to maintain lymphatic vascular integrity and regulate immune cell trafficking in obesity.

scholarly journals Application of wide-field infrared reflectance imaging in retinoschisis, retinal detachments, and schisis detachments

2019 ◽  
Vol 5 (S1) ◽  
Author(s):  
Himanshu K. Banda ◽  
Anjali Shah ◽  
Gaurav K. Shah
Keyword(s):  
Retinal Detachment ◽  
Near Infrared ◽  
Infrared Imaging ◽  
Anterior Segment ◽  
Infrared Light ◽  
Main Body ◽  
Wide Field ◽  
Wide Angle ◽  
Infrared Reflectance ◽  
Non Invasive

Abstract Background Retinoschisis and retinal detachment are distinguished based on features in clinical examination. Even to skilled examiners, some cases may be diagnostic challenges. Infrared and wide-angle infrared reflectance imaging are relatively new modalities that can provide additional diagnostic information. Non-contact infrared reflectance imaging (also described as near-infrared imaging) highlights sub-retinal features which may otherwise be obscured by standard retinal photography. It is non-invasive and uses the retina’s ability to absorb, reflect or scatter infrared light to produce high quality images. Main body The aim of this review is to describe the role of wide-field infrared imaging in screening, diagnosing, and monitoring structural peripheral retinal disorders including retinoschisis, retinal detachment or combined retinoschisis rhegmatogenous detachments. Infrared imaging can also be used to monitor anterior segment inflammation. Heidelberg Wide-Field Module lens and Heidelberg Spectralis® HRA + OCT machine (Heidelberg Engineering, Heidelberg, Germany) were used to obtain noncontact, wide-field infrared images on each study eye. Pseudocolor photos were captured by Optos Optomap® (Optos, Inc, Massachusetts, USA). Conclusion Wide angle infrared imaging offers a quick, noncontact, and noninvasive way to help specialists accurately diagnose, monitor for progression, and educate patients about retinal detachment, retinoschisis and even anterior segment inflammation.

scholarly journals Leukocyte Trafficking via Lymphatic Vessels in Atherosclerosis

Cells ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1344
Author(s):  
Kim Pin Yeo ◽  
Hwee Ying Lim ◽  
Veronique Angeli
Keyword(s):  
Immune Cell ◽  
Lymphatic Vessels ◽  
Cardiovascular Complications ◽  
Chronic Inflammatory Disease ◽  
Cell Trafficking ◽  
Functional Roles ◽  
New Findings ◽  
Lymphatic Vasculature ◽  
Acute And Chronic Inflammation ◽  
Progression Of Atherosclerosis

In recent years, lymphatic vessels have received increasing attention and our understanding of their development and functional roles in health and diseases has greatly improved. It has become clear that lymphatic vessels are critically involved in acute and chronic inflammation and its resolution by supporting the transport of immune cells, fluid, and macromolecules. As we will discuss in this review, the involvement of lymphatic vessels has been uncovered in atherosclerosis, a chronic inflammatory disease of medium- and large-sized arteries causing deadly cardiovascular complications worldwide. The progression of atherosclerosis is associated with morphological and functional alterations in lymphatic vessels draining the diseased artery. These defects in the lymphatic vasculature impact the inflammatory response in atherosclerosis by affecting immune cell trafficking, lymphoid neogenesis, and clearance of macromolecules in the arterial wall. Based on these new findings, we propose that targeting lymphatic function could be considered in conjunction with existing drugs as a treatment option for atherosclerosis.

scholarly journals An effective method for detecting dorsal hand veins utilising near-infrared imaging technology

2021 ◽  
Vol 63 (2) ◽  
pp. 32-38
Author(s):  
Hai Thanh Le ◽  
◽  
Hien Thi Thu Pham ◽  
Keyword(s):  
Near Infrared ◽  
Infrared Imaging ◽  
Blood Collection ◽  
Imaging Device ◽  
Non Invasive ◽  
Nir Imaging ◽  
Dorsal Hand ◽  
Dorsal Hand Vein ◽  
High Level ◽  
Level Process

Intravenous access for blood collection and other related therapies is one of the most frequently practiced procedures in the modern medical system. The procedure requires complex training and experience, as it might cause dangerous nerve damage and subcutaneous bleeding. This paper proposes a dorsal hand vein detection method utilising the near-infrared (NIR) imaging device to segment and visualise the subcutaneous vein patterns on the skin directly. Applying NIR light has received substantial attention because of its non-invasive and revealing substantially more information than the visible one. The proposed method is divided into the low- and high-level processes. The captured image is smoothed and enhanced to make the vein patterns clearer in the low-level process. The pre-processed image is then segmented step by step to extract the vein features and eliminate the pseudo-vein regions precisely. Lastly, the detected veins are thinned to reduce the thickness and projected back onto the acquired image in the high-level process. The proposed method performs effectively in detecting the clear dorsal hand veins through the experiment with a processing time of 0.61s for the high-resolution image.

scholarly journals When form meets function: the cells and signals that shape the lymphatic vasculature during development

Development ◽  
2021 ◽  
Vol 148 (11) ◽  
Author(s):  
Mathias Francois ◽  
Anna Oszmiana ◽  
Natasha L. Harvey
Keyword(s):  
Network Formation ◽  
Immune Cell ◽  
Lymphatic Endothelial Cell ◽  
Dietary Lipids ◽  
Cell Trafficking ◽  
Tissue Fluid ◽  
Cellular Events ◽  
Formation Function ◽  
Lymphatic Vasculature ◽  
Lymphatic Network

ABSTRACT The lymphatic vasculature is an integral component of the cardiovascular system. It is essential to maintain tissue fluid homeostasis, direct immune cell trafficking and absorb dietary lipids from the digestive tract. Major advances in our understanding of the genetic and cellular events important for constructing the lymphatic vasculature during development have recently been made. These include the identification of novel sources of lymphatic endothelial progenitor cells, the recognition of lymphatic endothelial cell specialisation and heterogeneity, and discovery of novel genes and signalling pathways underpinning developmental lymphangiogenesis. Here, we review these advances and discuss how they inform our understanding of lymphatic network formation, function and dysfunction.

scholarly journals A pilot clinical trial of a near‐infrared laser vaccine adjuvant: safety, tolerability, and cutaneous immune cell trafficking

2018 ◽  
Vol 33 (2) ◽  
pp. 3074-3081 ◽  
Author(s):  
Jeffrey A. Gelfand ◽  
Rosalynn M. Nazarian ◽  
Satoshi Kashiwagi ◽  
Timothy Brauns ◽  
Brent Martin ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Near Infrared ◽  
Immune Cell ◽  
Infrared Laser ◽  
Vaccine Adjuvant ◽  
Cell Trafficking ◽  
Immune Cell Trafficking
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