The Activation of Zebrafish Sperm Cells in a Micromixer

The freshwater fish, Danio rerio (zebrafish), have become widely used as a model organism for vertebrate development, DNA mutation, and human disease studies [1]. Maintaining live colonies of the numerous developed strains of zebrafish under investigation can be prohibitively costly. As such, there is a growing need to catalog their reproductive cells and have them available on demand [2]. Thus cryopreservation of model strain gametes has become an important endeavor, where evaluation of freezing and thawing techniques is currently a bottleneck to these procedures.

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The Importance of Zebrafish in Biomedical Research

Acta Médica Portuguesa ◽

10.20344/amp.4628 ◽

2013 ◽

Vol 26 (5) ◽

pp. 583

Author(s):

Bárbara Tavares ◽

Susana Santos Lopes

Keyword(s):

Biomedical Research ◽

Drug Screening ◽

Danio Rerio ◽

Scientific Literature ◽

Model Organism ◽

Toxicity Testing ◽

Vertebrate Development ◽

Automated Systems ◽

Ideal Model ◽

Research Drug

Introduction: Zebrafish (Danio rerio) is an ideal model organism for the study of vertebrate development. This is due to the large clutches that each couple produces, with up to 200 embryos every 7 days, and to the fact that the embryos and larvae are small, transparent and undergo rapid external development.Material and Methods: Using scientific literature research tools available online and the keywords Zebrafish, biomedical research, human disease, and drug screening, we reviewed original studies and reviews indexed in PubMed.Results: In this review we summarized work conducted with this model for the advancement of our knowledge related to several human diseases. We also focused on the biomedical research being performed in Portugal with the zebrafish model.Discussion: Powerful live imaging and genetic tools are currently available for zebrafish making it a valuable model in biomedicalresearch. The combination of these properties with the optimization of automated systems for drug screening has transformed the zebrafish into a “top model” in biomedical research, drug discovery and toxicity testing. Furthermore, with the optimization of xenografts technology it will be possible to use zebrafish to aide in the choice of the best therapy for each patient.Conclusion: Zebrafish is an excellent model organism in biomedical research, drug development and in clinical therapy.

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Antigenicity of sperm cells after freezing and thawing

Fertility and Sterility ◽

10.1016/s0015-0282(16)47787-3 ◽

1984 ◽

Vol 41 (4) ◽

pp. 615-619 ◽

Cited By ~ 5

Author(s):

Moshe Phillip ◽

Dita Kleinman ◽

Gad Potashnik ◽

Vaclav Insler

Keyword(s):

Sperm Cells ◽

Freezing And Thawing

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Differences in shoaling behavior in two species of freshwater fish (Danio rerio and Hyphessobrycon herbertaxelrodi).

Journal of Comparative Psychology ◽

10.1037/com0000041 ◽

2016 ◽

Vol 130 (4) ◽

pp. 358-368 ◽

Cited By ~ 6

Author(s):

Elisabet Gimeno ◽

Vicenç Quera ◽

Francesc S. Beltran ◽

Ruth Dolado

Keyword(s):

Freshwater Fish ◽

Danio Rerio ◽

Shoaling Behavior

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Toxicity and chronic effects of deltamethrin exposure on zebrafish (Danio rerio) as a reference model for freshwater fish community

Ecotoxicology and Environmental Safety ◽

10.1016/j.ecoenv.2019.01.057 ◽

2019 ◽

Vol 171 ◽

pp. 854-862 ◽

Cited By ~ 16

Author(s):

Stefan-Adrian Strungaru ◽

Gabriel Plavan ◽

Alin Ciobica ◽

Mircea Nicoara ◽

Madalina Andreea Robea ◽

...

Keyword(s):

Freshwater Fish ◽

Danio Rerio ◽

Fish Community ◽

Reference Model ◽

Chronic Effects

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Repression of Inappropriate Gene Expression in the Vertebrate Embryonic Ectoderm

Genes ◽

10.3390/genes10110895 ◽

2019 ◽

Vol 10 (11) ◽

pp. 895 ◽

Cited By ~ 1

Author(s):

Shoshana Reich ◽

Daniel C. Weinstein

Keyword(s):

Gene Expression ◽

Xenopus Laevis ◽

Cell Fate ◽

Regulation Of Gene Expression ◽

Model Organism ◽

Cell Fate Determination ◽

Vertebrate Development ◽

Germ Layers ◽

Inappropriate Gene Expression ◽

Embryonic Ectoderm

During vertebrate embryogenesis, precise regulation of gene expression is crucial for proper cell fate determination. Much of what we know about vertebrate development has been gleaned from experiments performed on embryos of the amphibian Xenopus laevis; this review will focus primarily on studies of this model organism. An early critical step during vertebrate development is the formation of the three primary germ layers—ectoderm, mesoderm, and endoderm—which emerge during the process of gastrulation. While much attention has been focused on the induction of mesoderm and endoderm, it has become clear that differentiation of the ectoderm involves more than the simple absence of inductive cues; rather, it additionally requires the inhibition of mesendoderm-promoting genes. This review aims to summarize our current understanding of the various inhibitors of inappropriate gene expression in the presumptive ectoderm.

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A highly divergent picornavirus infecting the gut epithelia of zebrafish (Danio rerio) in research institutions world-wide

10.1101/463083 ◽

2018 ◽

Author(s):

Eda Altan ◽

Steven V. Kubiski ◽

Ákos Boros ◽

Gábor Reuter ◽

Mohammadreza Sadeghi ◽

...

Keyword(s):

World Wide ◽

Natural Infection ◽

Model Organism ◽

Asymptomatic Infection ◽

Viral Metagenomics ◽

Vertebrate Development ◽

Wild Type ◽

Host Interactions ◽

Vertebrate Model

AbstractZebrafish have been extensively used as a model system for research in vertebrate development and pathogen-host interactions. We describe the complete genome of a novel picornavirus identified during a viral metagenomics analysis of zebrafish gut tissue. The closest relatives of this virus showed identity of ≤19.8% in their P1 capsids and ≤35.4% in their RdRp qualifying zebrafish picornavirus 1 (ZfPV1) as member of a novel genus with a proposed name of Cyprivirus. RT-PCR testing of zebrafish from 41 institutions from North America, Europe, and Asia showed ZfPV1 to be highly prevalent world-wide. In situ hybridization of whole zebrafish showed viral RNA was restricted to a subset of enterocytes and cells in the subjacent lamina propria of the intestine and the intestinal mucosa. This naturally occurring and apparently asymptomatic infection (in wild type zebrafish lineage AB) provides a natural infection system to study picornavirus-host interactions in an advanced vertebrate model organism. Whether ZfPV1 infection affects any immunological, developmental or other biological processes in wild type or mutant zebrafish lineages remains to be determined.

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Liver Alterations in Two Freshwater Fish Species (Carassius auratus and Danio rerio) Following Exposure to Different TiO2 Nanoparticle Concentrations

Microscopy and Microanalysis ◽

10.1017/s1431927613013238 ◽

2013 ◽

Vol 19 (5) ◽

pp. 1131-1140 ◽

Cited By ~ 23

Author(s):

Mário S. Diniz ◽

António P. Alves de Matos ◽

Joana Lourenço ◽

Luísa Castro ◽

Isabel Peres ◽

...

Keyword(s):

Oxidative Stress ◽

Freshwater Fish ◽

Fish Species ◽

Danio Rerio ◽

Carassius Auratus ◽

Tap Water ◽

Light And Electron Microscopy ◽

Partial Recovery ◽

Activity Increase ◽

Freshwater Fish Species

AbstractThe toxicity of titanium dioxide nanoparticles (TIO2 NPs) and oxidative stress effects were studied in two freshwater fish species (Carassius auratus and Danio rerio) exposed for 21 days to different concentrations (0.01, 0.1, 1, 10, 100/mgL) of TiO2 NPs and to a control (tap water). Additional fish were transferred to clean water for 14 days to assess the ability to recover from exposure to TiO2 NPs. Activities of the enzyme glutathione-S-transferase (GST) and lipid peroxidation (LPO) (malondialdheyde) were measured as indicators of oxidative stress. Histological and ultra-structural changes in livers from bothspecies of fish were evaluated by light and electron microscopy. Results show a general GST activity increase according to TiO2 NPs concentrations, which is in agreement with data from LPO. After 21 days, GST activities decreased possibly caused by suppression of GST synthesis as a result of severe stress. Histological and ultra-structural analysis of livers from exposed fish show degeneration of the hepatic tissue and alterations in hepatocytes such as glycogen depletion and an increase in lipofucsin lysosome-like granules. After a depuration period a partial recovery for biochemical markers and cells was observed. The results suggest that TiO2 promotes alterations in hepatic tissues compatible with oxidative stress.

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Copy number variation in the mouse genome: implications for the mouse as a model organism for human disease

Cytogenetic and Genome Research ◽

10.1159/000184721 ◽

2008 ◽

Vol 123 (1-4) ◽

pp. 297-306 ◽

Cited By ~ 16

Author(s):

G. Cutler ◽

P.D. Kassner

Keyword(s):

Copy Number Variation ◽

Human Disease ◽

Copy Number ◽

Mouse Genome ◽

Model Organism ◽

Number Variation

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The zebrafish klf gene family

Blood ◽

10.1182/blood.v98.6.1792 ◽

2001 ◽

Vol 98 (6) ◽

pp. 1792-1801 ◽

Cited By ~ 77

Author(s):

Andrew C. Oates ◽

Stephen J. Pratt ◽

Brenda Vail ◽

Yi-lin Yan ◽

Robert K. Ho ◽

...

Keyword(s):

Blood Vessel ◽

Gene Family ◽

Danio Rerio ◽

Expression Patterns ◽

Regulatory Proteins ◽

Developmental Expression ◽

Vertebrate Development ◽

Functional Conservation ◽

Transcriptional Regulatory ◽

High Degree

Abstract The Krüppel-like factor(KLF) family of genes encodes transcriptional regulatory proteins that play roles in differentiation of a diverse set of cells in mammals. For instance, the founding memberKLF1 (also known as EKLF) is required for normal globin production in mammals. Five new KLF genes have been isolated from the zebrafish, Danio rerio, and the structure of their products, their genetic map positions, and their expression during development of the zebrafish have been characterized. Three genes closely related to mammalian KLF2 andKLF4 were found, as was an ortholog of mammalianKLF12. A fifth gene, apparently missing from the genome of mammals and closely related to KLF1 and KLF2,was also identified. Analysis demonstrated the existence of novel conserved domains in the N-termini of these proteins. Developmental expression patterns suggest potential roles for these zebrafish genes in diverse processes, including hematopoiesis, blood vessel function, and fin and epidermal development. The studies imply a high degree of functional conservation of the zebrafish genes with their mammalian homologs. These findings further the understanding of theKLF genes in vertebrate development and indicate an ancient role in hematopoiesis for the Krüppel-like factorgene family.

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