scholarly journals Label-free fiber nanograting sensor for real-time in situ early monitoring of cellular apoptosis

2022 ◽  
Vol 4 (01) ◽  
Author(s):  
Danran Li ◽  
Nina Wang ◽  
Tianyang Zhang ◽  
Guangxing Wu ◽  
Yifeng Xiong ◽  
...  
Keyword(s):  
Real Time ◽  
Label Free ◽  
Cellular Apoptosis

Directly monitoring the active sites of charge transfer in heterocycles in situ and in real time

2019 ◽  
Vol 55 (4) ◽  
pp. 541-544 ◽  
Author(s):  
Shuji Ye ◽  
Junjun Tan ◽  
Kangzhen Tian ◽  
Chuanzhao Li ◽  
Jiahui Zhang ◽  
...  
Keyword(s):  
Charge Transfer ◽  
Real Time ◽  
Vibrational Spectroscopy ◽  
Active Sites ◽  
Electronic States ◽  
Sum Frequency Generation ◽  
Vibrational Modes ◽  
Label Free ◽  
Sum Frequency

Coherent degenerate infrared-infrared-visible sum frequency generation vibrational spectroscopy provides a powerful label-free probe for identifying the vibrational modes that are coupled through the electronic states in situ and in real time.

scholarly journals Cyclodextrin–polysaccharide-based, in situ-gelled system for ocular antifungal delivery

2014 ◽  
Vol 10 ◽  
pp. 2903-2911 ◽  
Author(s):  
Anxo Fernández-Ferreiro ◽  
Noelia Fernández Bargiela ◽  
María Santiago Varela ◽  
Maria Gil Martínez ◽  
Maria Pardo ◽  
...  
Keyword(s):  
Real Time ◽  
Inclusion Complexes ◽  
Gellan Gum ◽  
Effective Control ◽  
Cell Cytotoxicity ◽  
Label Free ◽  
Real Time Monitoring ◽  
Sulfobutyl Ether ◽  
Human Keratocytes

Fluconazole was studied with two different hydrophilic cyclodextrins (hydroxypropyl-β-cyclodextrin (HPBCD) and sulfobutyl ether-β-cyclodextrin (SBECD)) for the formation of inclusion complexes. HPBCD and SBECD showed low cell cytotoxicity in human keratocytes as assessed by the label-free xCELLigence system for real-time monitoring. The fluconazole–HPBCD complex was incorporated into an ion-sensitive ophthalmic gel composed of the natural polysaccharides gellan gum and κ-carrageenan. This system showed good bioadhesive properties and effective control of fluconazole release.

scholarly journals All-Fibre Label-Free Nano-Sensor for Real-Time in situ Early Monitoring of Cellular Apoptosis

2021 ◽  
Author(s):  
Dan-ran Li ◽  
Nina Wang ◽  
Tianyang Zhang ◽  
Guangxing Wu ◽  
Yifeng Xiong ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Label Free ◽  
Biochemical Engineering ◽  
Cellular Events ◽  
Environmental Variations ◽  
Cellular Apoptosis ◽  
Manufacturing Techniques ◽  
Apoptosis Process ◽  
Single Living Cells

Abstract The achievement of all-fibre functional nano-modules for subcellular label-free measurement has long been pursued due to the limitations of manufacturing techniques. In this paper, a compact all-fibre label-free nano-sensor composed of a fibre taper and zinc oxide nano-gratings is designed and applied for the early monitoring of apoptosis in single living cells. Because of its nanoscale dimensions, mechanical flexibility and minimal cytotoxicity to cells, the sensing module can be loaded in cells for long-term in situ tracking with high sensitivity. A gradual increase in the nuclear refractive index during the apoptosis process is observed, revealing the increase in molecular density and the decrease in cell volume. The strategy used in this study not only contributes to the understanding of internal environmental variations during cellular apoptosis but also provides a new platform for non-fluorescent all-fibre devices to investigate cellular events and to promote new progress in fundamental cell biochemical engineering.

Nanomechanical Microcantilevers for In-Vitro Biomolecular Detection

2007 ◽  
Author(s):  
Kilho Eom ◽  
Tae Yun Kwon ◽  
Jinsung Park ◽  
Sungsoo Na ◽  
Dae Sung Yoon ◽  
...  
Keyword(s):  
Real Time ◽  
Biological Molecules ◽  
Label Free ◽  
Biomolecular Detection ◽  
Label Free Detection ◽  
Detection Of Biomolecules ◽  
Kinetics Of ◽  
Insight Into

Nanomechanical microcantilevers have enabled the sensitive label-free detection of chemical and/or biological molecules. In recent years, resonating microcantilevers have achieved the unprecedented sensitivity in detecting molecules. In this article, we review our current works on the label-free detection of biomolecules based on resonating microcantilevers. Our piezoelectric thick film microcantilevers exhibit the relatively high quality factor in a viscous liquid, indicating the potential of our cantilever to in situ biosensor applications for real-time detection of biomolecular interactions. It is shown that our microcantilevers allow the noise-free real-time monitoring of biomolecular recognitions, providing the insight into kinetics of biomolecular recognitions.

scholarly journals Dynamic surface properties of PEG-coated CuS nanoparticles alter their interaction with cells as revealed by surface-enhanced infrared spectroscopy

2019 ◽  
Vol 1 (11) ◽  
pp. 4268-4276
Author(s):  
Fengjuan Cao ◽  
Lie Wu ◽  
Xiaofei Zhang ◽  
Shanshan Li ◽  
Chao Wang ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Real Time ◽  
Surface Properties ◽  
Cell Interaction ◽  
Label Free ◽  
Dynamic Changes ◽  
Dynamic Surface ◽  
Surface Enhanced ◽  
First Time

How the dynamic changes of the surface properties of nanoparticles affect the nano-cell interaction was revealed for the first time, by in situ real-time label-free SEIRA spectroscopy at the nanoscale.

Early apoptosis real-time detection by label-free SERS based on externalized phosphatidylserine

The Analyst ◽  
2016 ◽  
Vol 141 (14) ◽  
pp. 4293-4298 ◽  
Author(s):  
Haibo Zhou ◽  
Qiqin Wang ◽  
Detian Yuan ◽  
Jinyong Wang ◽  
Yang Huang ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Real Time ◽  
Label Free ◽  
Early Apoptosis ◽  
Real Time Detection

Early apoptosis real-time detection by label-free SERS based on externalized phosphatidylserine usingin situsynthesized silver nanoparticles.

In Situ Electrokinetic Preconcentrator for Conductive Biofluids

2009 ◽  
Author(s):  
Kai Yang ◽  
Jie Wu
Keyword(s):  
Diffusion Process ◽  
Real Time ◽  
Transport Process ◽  
Label Free ◽  
Detection Capability ◽  
Infectious Dose ◽  
Long Time

To detect and identify biomolecules at its infectious dose, all sensors require a relatively long time — days or even weeks — for reliable results. This delay occurs because the collection of analyte molecules onto the sensing elements depends solely on the diffusion process of those agents to a sensor. This work seeks to enhance the transport process, and thereby significantly improve a sensor’s detection capability. This work investigates an in-situ, label-free nanoparticle (e.g. protein) preconcentrator based on a new AC electrothermal effect (ACET) that can operate even in a highly conductive solution for which current methods such as dielectrophoresis, electrophresis or electroosmosis won’t function effectively. The proposed AC electrokinetic preconcentrator is expected to significantly increase the nanoparticle counts, realizing sub-femto molar detection in real time.

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