Nanomechanical Microcantilevers for In-Vitro Biomolecular Detection

2007 ◽  
Author(s):  
Kilho Eom ◽  
Tae Yun Kwon ◽  
Jinsung Park ◽  
Sungsoo Na ◽  
Dae Sung Yoon ◽  
...  
Keyword(s):  
Real Time ◽  
Biological Molecules ◽  
Label Free ◽  
Biomolecular Detection ◽  
Label Free Detection ◽  
Detection Of Biomolecules ◽  
Kinetics Of ◽  
Insight Into

Nanomechanical microcantilevers have enabled the sensitive label-free detection of chemical and/or biological molecules. In recent years, resonating microcantilevers have achieved the unprecedented sensitivity in detecting molecules. In this article, we review our current works on the label-free detection of biomolecules based on resonating microcantilevers. Our piezoelectric thick film microcantilevers exhibit the relatively high quality factor in a viscous liquid, indicating the potential of our cantilever to in situ biosensor applications for real-time detection of biomolecular interactions. It is shown that our microcantilevers allow the noise-free real-time monitoring of biomolecular recognitions, providing the insight into kinetics of biomolecular recognitions.

scholarly journals Noise Immune Dielectric Modulated Dual Trench Transparent Gate Engineered MOSFET as a Label Free Biosensor: Proposal and Investigation

2021 ◽  
Author(s):  
DIPANJAN SEN ◽  
Arpan De ◽  
Bijoy Goswami ◽  
Sharmistha Shee ◽  
Subir Kumar Sarkar
Keyword(s):  
High Frequency ◽  
Label Free ◽  
Threshold Voltage Shift ◽  
Enhanced Sensitivity ◽  
Label Free Detection ◽  
Drain Bias ◽  
Overall Performance ◽  
Detection Of Biomolecules ◽  
Insight Into ◽  
Positively Charged

Abstract In this work, we have examined and proposed a dielectrically modulated biosensor based on the dual trench transparent gate engineered MOSFET (DM DT GE-MOSFET) for label-free detection of biomolecules with enhanced sensitivity and efficiency. Different sensing parameters such as the ION/IOFF, threshold voltage shift have been evaluated to validate the sensing metric for the proposed device. Additionally, the SVth (Vth Sensitivity) has been also analyzed by considering the charged (positive and negative) biomolecules. In addition to this, the RF sensing parameters such as the transconductance gain and cut-off frequency have been also taken into account to provide a better insight into the sensitivity analysis of the proposed device. Furthermore, the linearity, distortion and noise immunity of the device has been evaluated to check the overall performance of the biosensor at high frequency (GHz). Moreover, the results indicate that, the proposed biosensor exhibits a SVth of 0.68 for the positively charged biomolecules at a very low drain bias (0.2 V). Therefore, the proposed device can be used as an alternative to the conventional FET-based biosensors.

scholarly journals Genetically Encoded Fluorescent Biosensors for Biomedical Applications

Biomedicines ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 1528
Author(s):  
Vera S. Ovechkina ◽  
Suren M. Zakian ◽  
Sergey P. Medvedev ◽  
Kamila R. Valetdinova
Keyword(s):  
Real Time ◽  
Biomedical Applications ◽  
Tissue Type ◽  
Drug Bioavailability ◽  
Modern Biology ◽  
Fluorescent Biosensors ◽  
Non Invasive ◽  
Insight Into

One of the challenges of modern biology and medicine is to visualize biomolecules in their natural environment, in real-time and in a non-invasive fashion, so as to gain insight into their physiological behavior and highlight alterations in pathological settings, which will enable to devise appropriate therapeutic strategies. Genetically encoded fluorescent biosensors constitute a class of imaging agents that enable visualization of biological processes and events directly in situ, preserving the native biological context and providing detailed insight into their localization and dynamics in cells. Real-time monitoring of drug action in a specific cellular compartment, organ, or tissue type; the ability to screen at the single-cell resolution; and the elimination of false-positive results caused by low drug bioavailability that is not detected by in vitro testing methods are a few of the obvious benefits of using genetically encoded fluorescent biosensors in drug screening. This review summarizes results of the studies that have been conducted in the last years toward the fabrication of genetically encoded fluorescent biosensors for biomedical applications with a comprehensive discussion on the challenges, future trends, and potential inputs needed for improving them.

Understanding the phase formation kinetics of nano-crystalline kesterite deposited on mesoscopic scaffolds via in situ multi-wavelength Raman-monitored annealing

2016 ◽  
Vol 18 (42) ◽  
pp. 29435-29446 ◽  
Author(s):  
Zhuoran Wang ◽  
Samir Elouatik ◽  
George P. Demopoulos
Keyword(s):  
Real Time ◽  
Phase Formation ◽  
Formation Kinetics ◽  
Nano Crystalline ◽  
Multi Wavelength ◽  
Real Time Information ◽  
Kinetics Of ◽  
Time Information ◽  
Annealing Method

The in situ Raman monitored annealing method is developed in this work to provide real-time information on phase formation and crystallinity evolution of kesterite deposited on a TiO2 mesoscopic scaffold.

Optimization of the nanofluidic design for label-free detection of biomolecules using a nanowall array

2017 ◽  
Vol 250 ◽  
pp. 39-43 ◽  
Author(s):  
Taiga Ajiri ◽  
Takao Yasui ◽  
Masatoshi Maeki ◽  
Akihiko Ishida ◽  
Hirofumi Tani ◽  
...  
Keyword(s):  
Label Free ◽  
Label Free Detection ◽  
Detection Of Biomolecules

scholarly journals Biologically sensitive field-effect transistors: from ISFETs to NanoFETs

2016 ◽  
Vol 60 (1) ◽  
pp. 81-90 ◽  
Author(s):  
Vivek Pachauri ◽  
Sven Ingebrandt
Keyword(s):  
Field Effect ◽  
Gate Dielectric ◽  
Gate Dielectrics ◽  
Field Effect Transistors ◽  
Silicon Nanowire ◽  
Label Free ◽  
Biomolecular Detection ◽  
New Device ◽  
Label Free Detection ◽  
History Of

Biologically sensitive field-effect transistors (BioFETs) are one of the most abundant classes of electronic sensors for biomolecular detection. Most of the time these sensors are realized as classical ion-sensitive field-effect transistors (ISFETs) having non-metallized gate dielectrics facing an electrolyte solution. In ISFETs, a semiconductor material is used as the active transducer element covered by a gate dielectric layer which is electronically sensitive to the (bio-)chemical changes that occur on its surface. This review will provide a brief overview of the history of ISFET biosensors with general operation concepts and sensing mechanisms. We also discuss silicon nanowire-based ISFETs (SiNW FETs) as the modern nanoscale version of classical ISFETs, as well as strategies to functionalize them with biologically sensitive layers. We include in our discussion other ISFET types based on nanomaterials such as carbon nanotubes, metal oxides and so on. The latest examples of highly sensitive label-free detection of deoxyribonucleic acid (DNA) molecules using SiNW FETs and single-cell recordings for drug screening and other applications of ISFETs will be highlighted. Finally, we suggest new device platforms and newly developed, miniaturized read-out tools with multichannel potentiometric and impedimetric measurement capabilities for future biomedical applications.

Y-shaped probe for convenient and label-free detection of microRNA-21 in vitro

2016 ◽  
Vol 499 ◽  
pp. 8-14 ◽  
Author(s):  
Kui He ◽  
Rong Liao ◽  
Changqun Cai ◽  
Caishuang Liang ◽  
Chan Liu ◽  
...  
Keyword(s):  
Label Free ◽  
Label Free Detection
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