Simple method of DNA stretching on glass substrate for fluorescence image and spectroscopy

Superhydrophobic self-cleaning surfaces are based on the surface micro/nanomorphologies and special chemical regents with low surface free energy. Such surface structures are highly susceptible to mechanical wear. Moreover, the use of fluorinated compounds is harmful to both human health and environment. Herein, a simple method based on spin coating and spraying is proposed to fabricate durable fluorine-free superhydrophobic surfaces. A glass substrate was coated with a layer of water-based paint via a simple spinning process. Due to the adhesive layer, modified SiO2 nanoparticles were strongly bonded to the glass substrate. After the coating was completely cured, the surfaces realized a water contact angle of 165.1° and maintained their water repellency after 50 abrasion cycles with sandpaper and 50 times of stripping test. No chemical reagents harmful for the environment were used.

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Simple Nanoimprint Lithography with 50 nm Resolution Using Vacuum Packing

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.459.111 ◽

2010 ◽

Vol 459 ◽

pp. 111-115 ◽

Cited By ~ 1

Author(s):

Kazuo Itoh ◽

Hirokazu Hirai ◽

Tetsuya Ando ◽

Kazuya Takagi ◽

Katsuya Noguchi

Keyword(s):

Aspect Ratio ◽

Line Width ◽

High Aspect Ratio ◽

Glass Substrate ◽

Atmospheric Pressure ◽

Nanoimprint Lithography ◽

Viscous Force ◽

Pressing Pressure ◽

Simple Method ◽

Fine Pattern

We demonstrated a simple UV nanoimprint method using vacuum packing. A glass substrate and a Si mold dropped with a photo-curable resist were vacuum-packed together in a packing sheet. They were pressed by atmospheric pressure and easily fixed without a complicated apparatus. We obtained a successful nanoimprint result for 100 nm width lines with high aspect ratio of 3.2. Further fine pattern of about 50 nm line width was also replicated using our simple method. The remaining thickness of the resist after imprint decreased with increasing the pressing pressure　whereas it saturated due to the viscosity of the resist. This saturation tendency is well explained by the balance between the pressing force and the viscous force of the resist.

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Preparation and characterization of silver orthophosphate photocatalytic coating on glass substrate

Scientific Reports ◽

10.1038/s41598-021-93352-z ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Masahide Hagiri ◽

Kenichi Uchida ◽

Mika Kamo Sasaki ◽

Shofiyah Sakinah

Keyword(s):

Visible Light ◽

Band Gap ◽

Glass Substrate ◽

Optical Band Gap ◽

X Ray Diffraction ◽

Simple Method ◽

Photocatalytic Ability ◽

X Ray ◽

Glass Substrates ◽

Elemental Silver

AbstractThe photocatalytic activity of silver orthophosphate Ag3PO4 has been studied and shown to have a high photo-oxidation capability. However, there is few reported example of a simple method to prepare Ag3PO4 coatings on various substrates. In this study a novel and simple method to immobilize Ag3PO4 on the surface of glass substrates has been developed. A silver phosphate paste based on a polyelectrolyte solution was applied to a smooth glass surface. The resulting dried material was calcined to obtain a coating that remained on the glass substrate. The coating layer was characterized by X-ray diffraction and energy dispersive X-ray spectrometry, and the optical band gap of the material was determined. The results indicated that an Ag3PO4 coating responsive to visible light was successfully prepared. The coating, under visible light irradiation, has the ability to decompose methylene blue. Although the coating contained some elemental silver, this did not adversely affect the optical band gap or the photocatalytic ability.

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A Simple Method to Improve Etching Uniformity when Making Phase Type CGHs on a Thick Glass Substrate

International Optical Design Conference and Optical Fabrication and Testing ◽

10.1364/oft.2010.owa2 ◽

2010 ◽

Cited By ~ 1

Author(s):

Quandou Wang ◽

Lei Chen ◽

Ulf Griesmann

Keyword(s):

Glass Substrate ◽

Simple Method ◽

Phase Type ◽

Thick Glass

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Effect of Annealing Temperature on Efficiency of Perovskite Solar Cell

Journal of Applied Science and Technology Trends ◽

10.38094/jastt1338 ◽

2020 ◽

Vol 1 (3) ◽

pp. 112-117

Author(s):

Issa Aziz ◽

Kamil Yousif ◽

Naseem Abdel

Keyword(s):

Solar Cells ◽

Glass Substrate ◽

Annealing Temperature ◽

Photovoltaic Performance ◽

Perovskite Solar Cells ◽

Lead Iodide ◽

Simple Method ◽

Coating Method ◽

The Stability ◽

Fto Glass

In this work, a simple method for producing Perovskite solar cells [PSCs] by recycling automobile batteries is used. Trying to get rid of some structures or materials which are harm to the environment (i.e. recycled car batteries) by using lead [Pb] sheets from those battery. Also, by reusing car batteries we will avoid the disposal of toxic battery elements and provide an alternative technique, readily-available Pb source for fabricating PSCs. Perovskite solar cells [PSCs] were prepared by two-step spin coating solution method grown on the FTO glass substrate. The organo-halide PSCs consists of four layers over FTO glass substrate. Lead iodide (PbI2) and methyl-ammonium iodide (CH3NH3I) used to form the structure of the precursor (CH3NH3PbI3) by the above-mentioned coating method. The photovoltaic performance of PSCs was investigated, together with the stability of PSCs, and the effect of annealing temperature of PRK layer on performance of PSCs. Characterization of PSCs achieved by using X-ray diffraction, SEM, and Spectrophotometer techniques. The effect of annealing on the optical properties of MAPbI3 films were studied by measuring spectral transmittance. The energy band gap value of the MAPbI3 film was found to be 1.60 eV.

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Benzylamine Tartrate as an Organic Glass Substrate for Carbon Films by Evaporation

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100069417 ◽

1970 ◽

Vol 28 ◽

pp. 484-485

Author(s):

A. C. Faberge

Keyword(s):

Vapor Pressure ◽

Viscous Liquid ◽

Glass Substrate ◽

Room Temperature ◽

Carbon Film ◽

Carbon Films ◽

Organic Glass ◽

Spectroscopic Examination ◽

Polymeric Substrates

Benzylamine tartrate (m.p. 63°C) seems to be a better and more convenient substrate for making carbon films than any of those previously proposed. Using it in the manner described, it is easy consistently to make batches of specimen grids as open as 200 mesh with no broken squares, and without individual handling of the grids. Benzylamine tartrate (hereafter called B.T.) is a viscous liquid when molten, which sets to a glass. Unlike polymeric substrates it does not swell before dissolving; such swelling of the substrate seems to be a principal cause of breakage of carbon film. Mass spectroscopic examination indicates a vapor pressure less than 10−9 Torr at room temperature.

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A simple way for producing holographic filters suitable for image improvement

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100108271 ◽

1978 ◽

Vol 36 (1) ◽

pp. 226-227

Author(s):

K.-H. Herrmann ◽

E. Reuber ◽

P. Schiske

Keyword(s):

Transfer Functions ◽

Diffraction Order ◽

Lateral Position ◽

Simple Method ◽

Spatial Frequencies ◽

Resolution Electron ◽

Wiener Filters ◽

Image Improvement ◽

Optical Reconstruction ◽

Set Up

Aposteriori deblurring of high resolution electron micrographs of weak phase objects can be performed by holographic filters [1,2] which are arranged in the Fourier domain of a light-optical reconstruction set-up. According to the diffraction efficiency and the lateral position of the grating structure, the filters permit adjustment of the amplitudes and phases of the spatial frequencies in the image which is obtained in the first diffraction order.In the case of bright field imaging with axial illumination, the Contrast Transfer Functions (CTF) are oscillating, but real. For different imageforming conditions and several signal-to-noise ratios an extensive set of Wiener-filters should be available. A simple method of producing such filters by only photographic and mechanical means will be described here.A transparent master grating with 6.25 lines/mm and 160 mm diameter was produced by a high precision computer plotter. It is photographed through a rotating mask, plotted by a standard plotter.

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Rotary Beveling for In Situ Thin-Section Microscopy of Cell Cultures

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100099192 ◽

1981 ◽

Vol 39 ◽

pp. 550-551

Author(s):

Dean A. Handley ◽

Jack T. Alexander ◽

Shu Chien

Keyword(s):

Cell Growth ◽

Cell Cultures ◽

Molecular Interactions ◽

Convenient Method ◽

Petri Dish ◽

Simple Method ◽

Thin Section Microscopy ◽

Thin Sectioning ◽

Organic Fluids

In situ preparation of cell cultures for ultrastructural investigations is a convenient method by which fixation, dehydration and embedment are carried out in the culture petri dish. The in situ method offers the advantage of preserving the native orientation of cell-cell interactions, junctional regions and overlapping configurations. In order to section after embedment, the petri dish is usually separated from the polymerized resin by either differential cryo-contraction or solvation in organic fluids. The remaining resin block must be re-embedded before sectioning. Although removal of the petri dish may not disrupt the native cellular geometry, it does sacrifice what is now recognized as an important characteristic of cell growth: cell-substratum molecular interactions. To preserve the topographic cell-substratum relationship, we developed a simple method of tapered rotary beveling to reduce the petri dish thickness to a dimension suitable for direct thin sectioning.

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