<title>Three-dimensional display and quantitative analysis of multidimensional light microscope images</title>

Interpretation of electron microscope images of crystalline lattice defects can be greatly aided by computer simulation of theoretical contrast from continuum models of such defects in thin foils. Several computer programs exist at the present time, but none are sufficiently general to permit their use as an aid in the identification of the range of defect types encountered in electron microscopy. This paper presents progress in the development of a more general computer program for this purpose which eliminates a number of restrictions contained in other programs. In particular, the program permits a variety of foil geometries and defect types to be simulated.The conventional approximation of non-interacting columns is employed for evaluation of the two-beam dynamical scattering equations by a piecewise solution of the Howie-Whelan equations.

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Improved 3-D reconstruction using stereo computer graphics and multiple tilt EM images

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100139524 ◽

1995 ◽

Vol 53 ◽

pp. 630-631

Author(s):

Lee D. Peachey ◽

Lou Fodor ◽

John C. Haselgrove ◽

Stanley M. Dunn ◽

Junqing Huang

Keyword(s):

Computer Graphics ◽

High Performance ◽

Transverse Section ◽

Three Dimensional ◽

Stereo Pair ◽

3D Reconstructions ◽

Video Cameras ◽

Biological Objects ◽

Microscope Images ◽

High Performance Computer

Stereo pairs of electron microscope images provide valuable visual impressions of the three-dimensional nature of specimens, including biological objects. Beyond this one seeks quantitatively accurate models and measurements of the three dimensional positions and sizes of structures in the specimen. In our laboratory, we have sought to combine high resolution video cameras with high performance computer graphics systems to improve both the ease of building 3D reconstructions and the accuracy of 3D measurements, by using multiple tilt images of the same specimen tilted over a wider range of angles than can be viewed stereoscopically. Ultimately we also wish to automate the reconstruction and measurement process, and have initiated work in that direction.Figure 1 is a stereo pair of 400 kV images from a 1 micrometer thick transverse section of frog skeletal muscle stained with the Golgi stain. This stain selectively increases the density of the transverse tubular network in these muscle cells, and it is this network that we reconstruct in this example.

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Study on erythrocytes by SEM photogrammetry (SEMP) technique

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100161187 ◽

1990 ◽

Vol 48 (3) ◽

pp. 724-725

Author(s):

Tong Wensheng ◽

Lu Lianhuang ◽

Zhang Zhijun

Keyword(s):

Quantitative Analysis ◽

Cell Membrane ◽

Clinical Diagnosis ◽

Human Body ◽

Blood Cells ◽

Three Dimensional ◽

Normal Person ◽

Blood Disease ◽

Computation Technique ◽

Important Basis

This is a combined study of two diffirent branches, photogrammetry and morphology of blood cells. The three dimensional quantitative analysis of erythrocytes using SEMP technique, electron computation technique and photogrammetry theory has made it possible to push the study of mophology of blood cells from LM, TEM, SEM to a higher stage, that of SEM P. A new path has been broken for deeply study of morphology of blood cells.In medical view, the abnormality of the quality and quantity of erythrocytes is one of the important changes of blood disease. It shows the abnormal blood—making function of the human body. Therefore, the study of the change of shape on erythrocytes is the indispensable and important basis of reference in the clinical diagnosis and research of blood disease.The erythrocytes of one normal person, three PNH Patients and one AA patient were used in this experiment. This research determines the following items: Height;Length of two axes (long and short), ratio; Crevice in depth and width of cell membrane; Circumference of erythrocytes; Isoline map of erythrocytes; Section map of erythrocytes.

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Three-Dimensional Subcellular Organization of Hepatocytes in Intact Liver: Simultaneous Visualization of Cytoskeletal and Membrane Markers by Confocal Microscopy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100163903 ◽

1996 ◽

Vol 54 ◽

pp. 288-289

Author(s):

Greg V. Martin ◽

Ann L. Hubbard

Keyword(s):

Three Dimensional ◽

Integral Membrane Proteins ◽

Polarized Secretion ◽

Microscope Images ◽

Computer Aided ◽

Intracellular Organelles ◽

Cytoskeletal Elements ◽

Simultaneous Visualization

The microtubule (MT) cytoskeleton is necessary for many of the polarized functions of hepatocytes. Among the functions dependent on the MT-based cytoskeleton are polarized secretion of proteins, delivery of endocytosed material to lysosomes, and transcytosis of integral plasma membrane (PM) proteins. Although microtubules have been shown to be crucial to the establishment and maintenance of functional and structural polarization in the hepatocyte, little is known about the architecture of the hepatocyte MT cytoskeleton in vivo, particularly with regard to its relationship to PM domains and membranous organelles. Using an in situ extraction technique that preserves both microtubules and cellular membranes, we have developed a protocol for immunofluorescent co-localization of cytoskeletal elements and integral membrane proteins within 20 µm cryosections of fixed rat liver. Computer-aided 3D reconstruction of multi-spectral confocal microscope images was used to visualize the spatial relationships among the MT cytoskeleton, PM domains and intracellular organelles.

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Dynamic simulation of wind field in three-dimensional display space considering particle swarm algorithm fuzzy logic

Journal of Intelligent & Fuzzy Systems ◽

10.3233/jifs-189977 ◽

2021 ◽

pp. 1-6

Author(s):

Honglei Xu ◽

Linhuan Wang

Keyword(s):

Fuzzy Logic ◽

Dynamic Simulation ◽

Wind Field ◽

Three Dimensional ◽

Measurement Data ◽

Particle Swarm ◽

Particle Swarm Algorithm ◽

Three Dimensional Display ◽

Swarm Algorithm ◽

Synchronous Measurement

In order to improve the accuracy of dynamic detection of wind field in the three-dimensional display space, system software is carried out on the actual scene and corresponding airborne radar observation information data, and the particle swarm algorithm fuzzy logic algorithm is introduced into the wind field dynamic simulation process in three-dimensional display space, to analyze the error of the filtering result in detail, to process the hurricane Lily Doppler radar measurement data with the optimal adaptive filtering according to the error data. The three-dimensional wind field synchronous measurement data obtained by filtering was compared with three-dimensional wind field synchronous measurement data of the GPS dropsonde in this experiment, the sea surface wind field measurement data of the multi-band microwave radiometer, and the wind field data at aircraft altitude.

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