scholarly journals Characterization of pKP-M1144, a Novel ColE1-Like Plasmid Encoding IMP-8, GES-5, and BEL-1 β-Lactamases, from a Klebsiella pneumoniae Sequence Type 252 Isolate

2015 ◽  
Vol 59 (8) ◽  
pp. 5065-5068 ◽  
Author(s):  
Costas C. Papagiannitsis ◽  
Monika Dolejska ◽  
Radoslaw Izdebski ◽  
Hana Dobiasova ◽  
Vendula Studentova ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Gene Cassette ◽  
Interesting Case ◽  
Sequence Type ◽  
Gram Negative Bacteria ◽  
Content Type ◽  
Resistance Determinants ◽  
Evolution Of Resistance ◽  
Replication Region

ABSTRACTIMP-8 metallo-β-lactamase was identified inKlebsiella pneumoniaesequence type 252 (ST252), isolated in a Portuguese hospital in 2009.blaIMP-8was the first gene cassette of a novel class 3 integron, In1144, also carrying theblaGES-5,blaBEL-1, andaacA4cassettes. In1144 was located on a ColE1-like plasmid, pKP-M1144 (12,029 bp), with a replication region of limited nucleotide similarity to those of other RNA-priming plasmids, such as pJHCMW1. In1144 and pKP-M1144 represent an interesting case of evolution of resistance determinants in Gram-negative bacteria.

scholarly journals Characterization of Metallo-β-Lactamase VIM-27, an A57S Mutant of VIM-1 Associated with Klebsiella pneumoniae ST147

2011 ◽  
Vol 55 (7) ◽  
pp. 3570-3572 ◽  
Author(s):  
C. C. Papagiannitsis ◽  
S. D. Kotsakis ◽  
E. Petinaki ◽  
A. C. Vatopoulos ◽  
E. Tzelepi ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Kinetic Parameters ◽  
Sequence Type ◽  
Class 1 Integron ◽  
Content Type ◽  
Class 1

ABSTRACTVIM-27 metallo-β-lactamase, an Ala57→ Ser variant of VIM-1, was identified in threeKlebsiella pneumoniaeisolates belonging to sequence type 147.blaVIM-27was part of a class 1 integron carried by non-self-transferable plasmids. Kinetic parameters and MIC determinations indicated that VIM-27 hydrolyzed most β-lactams, especially imipenem and cefoxitin, less effectively than VIM-1.

scholarly journals Characterization of pKP1433, a Novel KPC-2-Encoding Plasmid from Klebsiella pneumoniae Sequence Type 340

2013 ◽  
Vol 57 (7) ◽  
pp. 3427-3429 ◽  
Author(s):  
C. C. Papagiannitsis ◽  
V. Miriagou ◽  
P. Giakkoupi ◽  
L. S. Tzouvelekis ◽  
A. C. Vatopoulos
Keyword(s):  
Nucleotide Sequence ◽  
Klebsiella Pneumoniae ◽  
Sequence Type ◽  
Content Type ◽  
Extensive Sequence

ABSTRACTThe nucleotide sequence of pKP1433 (55,417 bp), ablaKPC-2-carrying plasmid fromKlebsiella pneumoniaesequence type 340, was determined. pKP1433 displayed extensive sequence and structural similarities with the IncN plasmids possessing the KPC-2-encoding Tn4401bisoform. However, the replication, partitioning, and stability of pKP1433 were determined by sequences related to diverse non-IncN plasmids.

scholarly journals Extended-Spectrum β-Lactamase CTX-M-15-Producing Klebsiella pneumoniae of Sequence Type ST274 in Companion Animals

2013 ◽  
Vol 57 (5) ◽  
pp. 2372-2375 ◽  
Author(s):  
Laurent Poirel ◽  
Patrice Nordmann ◽  
Sébastien Ducroz ◽  
Henri-Jean Boulouis ◽  
Pascal Arné ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Companion Animals ◽  
Sequence Type ◽  
High Rate ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Content Type ◽  
Extended Spectrum ◽  
Paris Area

ABSTRACTScreening of extended-spectrum β-lactamase (ESBL)-producing Gram-negative bacteria in companion animals living in the Paris area in France identified a high rate of CTX-M-15-producingKlebsiella pneumoniae. Those isolates were recovered during the 2010-2011 period from both infections and asymptomatic colonizations. Sequence typing revealed that most of these isolates belonged to sequence type ST274. Interestingly, theblaCTX-M-15gene was located on a specific and novel plasmid scaffold. These findings highlight that companion animals may be reservoirs for CTX-M-15-producingK. pneumoniaeevolving separately from the human reservoir of CTX-M-15 producers.

scholarly journals Carbapenemase-Producing Gram-Negative Bacteria from American Crows in the United States

2020 ◽  
Vol 65 (1) ◽  
pp. e00586-20
Author(s):  
Iva Kutilova ◽  
Adam Valcek ◽  
Costas C. Papagiannitsis ◽  
Darina Cejkova ◽  
Martina Masarikova ◽  
...  
Keyword(s):  
United States ◽  
Klebsiella Pneumoniae ◽  
Gene Cassette ◽  
The United States ◽  
Gram Negative Bacteria ◽  
Providencia Rettgeri ◽  
Gram Negative ◽  
Fecal Samples ◽  
Content Type ◽  
American Crows

ABSTRACTWild corvids were examined for the presence of carbapenemase-producing Gram-negative bacteria in the United States. A total of 13 isolates were detected among 590 fecal samples of American crow; 11 Providencia rettgeri isolates harboring blaIMP-27 on the chromosome as a class 2 integron gene cassette within the Tn7 transposon, 1 Klebsiella pneumoniae ST258 isolate carrying blaKPC-2 on a pKpQIL-like plasmid as a part of Tn4401a, and 1 Enterobacter bugandensis isolate with blaIMI-1 located within EcloIMEX-2.

scholarly journals Whole-Genome Sequence of a Novel Sequence Type 3136 Carbapenem-Resistant Klebsiella pneumoniae Strain Isolated from a Hospitalized Patient in Durban, South Africa

2018 ◽  
Vol 7 (23) ◽  
Author(s):  
Yogandree Ramsamy ◽  
Koleka P. Mlisana ◽  
Mushal Allam ◽  
Arshad Ismail ◽  
Ravesh Singh ◽  
...  
Keyword(s):  
South Africa ◽  
Klebsiella Pneumoniae ◽  
Virulence Factors ◽  
Genome Sequence ◽  
Sequence Type ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Content Type ◽  
Resistance Determinants ◽  
Carbapenem Resistant

Here, we describe the genome sequence of a novel sequence type 3136 (ST3136) Klebsiella pneumoniae strain isolated in South Africa. The 5,574,236-bp genome harbored 23 resistance determinants and 12 virulence factors that are of cardinal importance to infections.

scholarly journals Complete Sequence of a Novel IncR-F33:A–:B– Plasmid, pKP1034, HarboringfosA3,blaKPC-2,blaCTX-M-65,blaSHV-12, andrmtBfrom an Epidemic Klebsiella pneumoniae Sequence Type 11 Strain in China

2015 ◽  
Vol 60 (3) ◽  
pp. 1343-1348 ◽  
Author(s):  
Dai-Rong Xiang ◽  
Jun-Jie Li ◽  
Zi-Ke Sheng ◽  
Hai-Ying Yu ◽  
Mei Deng ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Mainland China ◽  
Complete Sequence ◽  
Resistance Rate ◽  
Sequence Type ◽  
University Hospital ◽  
Content Type ◽  
Resistance Determinants ◽  
Fosfomycin Resistance ◽  
Positive Isolate

A high fosfomycin resistance rate was observed inKlebsiella pneumoniaecarbapenemase (KPC)-producingK. pneumoniae(KPC-KP) in our previous study, but little is known about its mechanisms. In this study, we explored the prevalence of plasmid-mediated fosfomycin resistance determinants among fosfomycin-resistant KPC-KP strains from a Chinese university hospital and determined the complete sequence of a novelfosA3-carrying plasmid isolated from an epidemicK. pneumoniaesequence type (ST) 11 strain. A total of 97 KPC-KP strains were studied, of which 57 (58.8%) were resistant to fosfomycin, including 44 (45.4%) harboringfosA3and 1 harboringfosA. AllfosA3-positive strains belonged to the dominant ST11-pulse type (PT) A clone according to multilocus sequence typing and pulsed-field gel electrophoresis, suggesting clonal dissemination. ThefosA-positive isolate belonged to ST11-PTE. ThefosA3-carrying plasmid pKP1034 is 136,848 bp in length and is not self-transmissible. It is a multireplicon plasmid belonging to IncR-F33:A−: B−. BesidesfosA3, a variety of other resistance determinants, includingblaKPC-2,rmtB,blaCTX-M-65, andblaSHV-12, are identified in pKP1034, which would allow for coselection offosA3by most β-lactams and/or aminoglycosides and facilitate its dissemination despite limited use of fosfomycin in China. Detailed comparisons with related plasmids revealed that pKP1034 is highly mosaic and might have evolved from alarming recombination of theblaKPC-2-carrying plasmid pKPC-LK30 from Taiwan and the epidemicfosA3-carrying plasmid pHN7A8 from mainland China.

scholarly journals Characterization of a Multidrug-Resistant Porcine Klebsiella pneumoniae Sequence Type 11 Strain Coharboring blaKPC-2 and fosA3 on Two Novel Hybrid Plasmids

mSphere ◽  
2019 ◽  
Vol 4 (5) ◽  
Author(s):  
Wanjiang Zhang ◽  
Yao Zhu ◽  
Changzhen Wang ◽  
Wenyu Liu ◽  
Ruichao Li ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Resistance Genes ◽  
Plasmid Stability ◽  
Sequence Type ◽  
Animal Origin ◽  
Target Sequence ◽  
Human Origin ◽  
Content Type ◽  
Isolation And Characterization

ABSTRACT The occurrence of carbapenemase-producing Enterobacteriaceae (CPE) poses a considerable risk for public health. The gene for Klebsiella pneumoniae carbapenemase-2 (KPC-2) has been reported in many countries worldwide, and KPC-2-producing strains are mainly of human origin. In this study, we identified two novel hybrid plasmids that carry either blaKPC-2 or the fosfomycin resistance gene fosA3 in the multiresistant K. pneumoniae isolate K15 of swine origin in China. The blaKPC-2-bearing plasmid pK15-KPC was a fusion derivative of an IncF33:A−:B− incompatibility group (Inc) plasmid and chromosomal sequences of K. pneumoniae (CSKP). A 5-bp direct target sequence duplication (GACTA) was identified at the boundaries of the CSKP, suggesting that the integration might have been due to a transposition event. The blaKPC-2 gene on pK15-KPC was in a derivative of ΔTn6296-1. The multireplicon fosA3-carrying IncN-IncR plasmid pK15-FOS also showed a mosaic structure, possibly originating from a recombination between an epidemic fosA3-carrying pHN7A8-like plasmid and a pKPC-LK30-like IncR plasmid. Stability tests demonstrated that both novel hybrid plasmids were stably maintained in the original host without antibiotic selection but were lost from the transformants after approximately 200 generations. This is apparently the first description of a porcine sequence type 11 (ST11) K. pneumoniae isolate coproducing KPC-2 and FosA3 via pK15-KPC and pK15-FOS, respectively. The multidrug resistance (MDR) phenotype of this high-risk K. pneumoniae isolate may contribute to its spread and its persistence. IMPORTANCE The global dissemination of carbapenem resistance genes is of great concern. Animals are usually considered a reservoir of resistance genes and an important source of human infection. Although carbapenemase-producing Enterobacteriaceae strains of animal origin have been reported increasingly, blaKPC-2-positive strains from food-producing animals are still rare. In this study, we first describe the isolation and characterization of a carbapenem-resistant Klebsiella pneumoniae ST11 isolate, strain K15, which is of pig origin and coproduces KPC-2 and FosA3 via two novel hybrid plasmids. Furthermore, our findings highlight that this ST11 Klebsiella pneumoniae strain K15 is most likely of human origin and could be easily transmitted back to humans via direct contact or food intake. In light of our findings, significant attention must be paid to monitoring the prevalence and further evolution of blaKPC-2-carrying plasmids among the Enterobacteriaceae strains of animal origin.

scholarly journals Third-Generation-Cephalosporin-Resistant Klebsiella pneumoniae Isolates from Humans and Companion Animals in Switzerland: Spread of a DHA-Producing Sequence Type 11 Clone in a Veterinary Setting

2015 ◽  
Vol 59 (5) ◽  
pp. 2949-2955 ◽  
Author(s):  
Nadia Wohlwend ◽  
Andrea Endimiani ◽  
Thierry Francey ◽  
Vincent Perreten
Keyword(s):  
Klebsiella Pneumoniae ◽  
Companion Animals ◽  
Generation Cephalosporin ◽  
Sequence Type ◽  
Third Generation ◽  
Content Type ◽  
Human Infections ◽  
Veterinary Hospital ◽  
Animal Isolates

ABSTRACTCharacterization of third-generation-cephalosporin-resistantKlebsiella pneumoniaeisolates originating mainly from one human hospital (n= 22) and one companion animal hospital (n= 25) in Bern (Switzerland) revealed the absence of epidemiological links between human and animal isolates. Human infections were not associated with the spread of any specific clone, while the majority of animal infections were due toK. pneumoniaesequence type 11 isolates producing plasmidic DHA AmpC. This clonal dissemination within the veterinary hospital emphasizes the need for effective infection control practices.

scholarly journals Biochemical Characterization of VIM-39, a VIM-1-Like Metallo-β-Lactamase Variant from a Multidrug-Resistant Klebsiella pneumoniae Isolate from Greece

2015 ◽  
Vol 59 (12) ◽  
pp. 7811-7814 ◽  
Author(s):  
Costas C. Papagiannitsis ◽  
Simona Pollini ◽  
Filomena De Luca ◽  
Gian Maria Rossolini ◽  
Jean-Denis Docquier ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Clinical Isolate ◽  
Biochemical Characterization ◽  
Multidrug Resistant ◽  
Sequence Type ◽  
Turnover Rates ◽  
Content Type

ABSTRACTVIM-39, a VIM-1-like metallo-β-lactamase variant (VIM-1 Thr33Ala His224Leu) was identified in a clinical isolate ofKlebsiella pneumoniaebelonging to sequence type 147. VIM-39 hydrolyzed ampicillin, cephalothin, and imipenem more efficiently than did VIM-1 and VIM-26 (a VIM-1 variant with the His224Leu substitution) because of higher turnover rates.

scholarly journals Characterization of Tn6238with a New Allele ofaac(6′)-Ib-cr

2015 ◽  
Vol 59 (5) ◽  
pp. 2893-2897 ◽  
Author(s):  
María P. Quiroga ◽  
Betina Orman ◽  
Laura Errecalde ◽  
Sara Kaufman ◽  
Daniela Centrón
Keyword(s):  
Genetic Structure ◽  
Homologous Recombination ◽  
Klebsiella Pneumoniae ◽  
Clinical Isolate ◽  
Bioinformatic Analysis ◽  
Fluoroquinolone Resistance ◽  
Content Type ◽  
Gene Cassettes ◽  
Resistance Determinants

ABSTRACTHere, we report that the genetic structure of Tn1331remained conserved in Argentina from 1989 to 2013 (72 of 73 isolates), with the exception being the plasmid-borne Tn1331-like transposon Tn6238containing a newaac(6′)-Ib-crallele recovered from a colistin-resistantKlebsiella pneumoniaeclinical isolate. A bioinformatic analysis ofaac(6′)-Ib-like gene cassettes suggests that this newaac(6′)-Ib-crallele emerged through mutation or homologous recombination in the Tn1331genetic platform. Tn6238is a novel platform for the dissemination of aminoglycoside and fluoroquinolone resistance determinants.

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