Isolation of VIM-2-Producing Pseudomonas monteilii Clinical Strains Disseminated in a Tertiary Hospital in Northern Spain

ABSTRACTWe describe here the occurrence ofblaVIM-2in 10 carbapenem-resistantPseudomonas monteiliistrains isolated from different clinical samples from patients at the University Hospital Marqués de Valdecilla in northern Spain. All theblaVIM-2-harboringP. monteiliiisolates possessed a class 1 integron, with the cassette array [intI1_blaVIM-2_aac(6′)-Ib_qacEΔ1_sul1]. Our results show the emergence of VIM-2-producing multidrug-resistant species other thanPseudomonas aeruginosaorPseudomonas putidain a Spanish hospital.P. monteilii, although sporadically isolated, should also be considered an important metallo-β-lactamase (MBL) reservoir.

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Dissemination of Multidrug-Resistant Proteus mirabilis Clones Carrying a Novel Integron-Borne blaIMP-1 in a Tertiary Hospital

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.01321-17 ◽

2017 ◽

Vol 62 (2) ◽

Cited By ~ 3

Author(s):

Ana Carolina Ramos ◽

Rodrigo Cayô ◽

Cecilia Godoy Carvalhaes ◽

Thomas Jové ◽

Greice Pereira da Silva ◽

...

Keyword(s):

Infection Control ◽

Proteus Mirabilis ◽

Tertiary Hospital ◽

Multidrug Resistant ◽

Conjugative Plasmid ◽

Class 1 Integron ◽

Content Type ◽

Class 1

ABSTRACT This study aimed to characterize multidrug-resistant Proteus mirabilis clones carrying a novel class 1 integron-borne bla IMP-1. In1359 was inserted into a large conjugative plasmid that also carried bla CTX-M-2. The production of carbapenemases in Enterobacteriaceae that are intrinsically resistant to polymyxins and tigecycline is very worrisome, representing a serious challenge to clinicians and infection control teams.

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A Multispecies Cluster of VIM-1 Carbapenemase-Producing Enterobacterales Linked by a Novel, Highly Conjugative, and Broad-Host-Range IncA Plasmid Forebodes the Reemergence of VIM-1

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.02435-19 ◽

2020 ◽

Vol 64 (4) ◽

Cited By ~ 2

Author(s):

Gabriele Arcari ◽

Federica Maria Di Lella ◽

Giulia Bibbolino ◽

Fabio Mengoni ◽

Marzia Beccaccioli ◽

...

Keyword(s):

Host Range ◽

Bacterial Species ◽

Klebsiella Oxytoca ◽

Gene Cassette ◽

University Hospital ◽

Broad Host Range ◽

Class 1 Integron ◽

Content Type ◽

Carbapenem Resistant ◽

Class 1

ABSTRACT In this study, we investigated VIM-1-producing Escherichia coli, Klebsiella oxytoca, Klebsiella pneumoniae, Citrobacter freundii, and Enterobacter cloacae strains, isolated in 2019 during a period of active surveillance of carbapenem-resistant Enterobacterales in a large university hospital in Italy. VIM-1-producing strains colonized the gut of patients, with up to three different VIM-1-positive bacterial species isolated from a single rectal swab, but also caused bloodstream infection in one colonized patient. In the multispecies cluster, blaVIM-1 was identified in a 5-gene cassette class 1 integron, associated with several genetic determinants, including the blaSHV-12, qnrS1, and mph(A) genes, located on a highly conjugative and broad-host-range IncA plasmid. The characteristics and origin of this IncA plasmid were studied.

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Diversity and Evolution of AbaR Genomic Resistance Islands in Acinetobacter baumannii Strains of European Clone I

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.00221-11 ◽

2011 ◽

Vol 55 (7) ◽

pp. 3201-3206 ◽

Cited By ~ 54

Author(s):

Lenka Krizova ◽

Lenie Dijkshoorn ◽

Alexandr Nemec

Keyword(s):

Acinetobacter Baumannii ◽

Selective Advantage ◽

Multidrug Resistant ◽

Original Form ◽

Class 1 Integron ◽

Content Type ◽

Atpase Gene ◽

Class 1 ◽

Major Clone ◽

Derivatives Of

ABSTRACTTo assess the diversity of AbaR genomic resistance islands inAcinetobacter baumanniiEuropean clone I (MLST clonal complex 1), we investigated 26 multidrug-resistant strains of this major clone isolated from hospitals in 21 cities of 10 European countries between 1984 and 2005. Each strain harbored an AbaR structure integrated at the same position in the chromosomal ATPase gene. AbaR3, including four subtypes based on variations in class 1 integron cassettes, and AbaR10 were found in 15 and 2 strains, respectively, whereas a new, unique AbaR variant was discovered in each of the other 9 strains. These new variants, designated AbaR11 to AbaR19 (19.8 kb to 57.5 kb), seem to be truncated derivatives of AbaR3, likely resulting from the deletions of its internal parts mediated by either IS26elements (AbaR12 to AbaR19) or homologous recombination (AbaR11). AbaR3 was detected in all 10 strains isolated in 1984 to 1991, while AbaR11 to AbaR19 were carried only by strains isolated since 1997. Our results and those from previous publications suggest that AbaR3 is the original form of AbaR in European clone I, which may have provided strains of the lineage with a selective advantage facilitating their spread in European hospitals in the 1980s or before.

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Comparative Sequence Analysis of a Multidrug-Resistant Plasmid from Aeromonas hydrophila

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.01239-12 ◽

2012 ◽

Vol 57 (1) ◽

pp. 120-129 ◽

Cited By ~ 56

Author(s):

Carmelo S. del Castillo ◽

Jun-ichi Hikima ◽

Ho-Bin Jang ◽

Seong-Won Nho ◽

Tae-Sung Jung ◽

...

Keyword(s):

Drug Resistance ◽

Aeromonas Hydrophila ◽

Multidrug Resistant ◽

Comparative Sequence Analysis ◽

Human Pathogen ◽

Class 1 Integron ◽

Content Type ◽

Coding Regions ◽

Class 1 ◽

Complete Set

ABSTRACTAeromonas hydrophilais a pathogenic bacterium that has been implicated in fish, animal, and human disease. Recently, a multidrug resistance (MDR) plasmid, pR148, was isolated fromA. hydrophilaobtained from a tilapia (Oreochromis niloticus) farm in Thailand. pR148 is a 165,906-bp circular plasmid containing 147 coding regions showing highest similarity to pNDM-1_Dok1, an MDR plasmid isolated from a human pathogen. pR148 was also very similar to other IncA/C plasmids isolated from humans, animals, food, and fish. pR148 contains a mercuric resistance operon and encodes the complete set of genes for the type 4 secretion system. pR148 encodes a Tn21type transposon. This transposon contains the drug resistance genesqacH,blaOXA-10,aadA1, andsul1in a class 1 integron;tetAandtetRin transposon Tn1721; andcatA2and a duplicatesul1in a locus showing 100% similarity to IncU plasmids isolated from fish. TheblaOXA-10andaadA1genes showed 100% similarity to those from theAcinetobacter baumanniiAYE genome. The similarity of pR148 to a human pathogen-derived plasmid indicates that the plasmids were either transferred between different genera or that they are derived from a common origin. Previous studies have shown that IncA/C plasmids retain a conserved backbone, while the accessory region points to lateral gene transfer. These observations point out the dangers of indiscriminate use of antibiotics in humans and in animals and the necessity of understanding how drug resistance determinants are disseminated and transferred.

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Environmental Microbiota Represents a Natural Reservoir for Dissemination of Clinically Relevant Metallo-β-Lactamases

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.00716-11 ◽

2011 ◽

Vol 55 (11) ◽

pp. 5376-5379 ◽

Cited By ~ 33

Author(s):

Claudia Scotta ◽

Carlos Juan ◽

Gabriel Cabot ◽

Antonio Oliver ◽

Jorge Lalucat ◽

...

Keyword(s):

Sewage Water ◽

Ochrobactrum Anthropi ◽

Class 1 Integron ◽

Content Type ◽

Natural Reservoir ◽

Brevundimonas Diminuta ◽

Pseudomonas Monteilii ◽

Class 1 ◽

Enterobacter Ludwigii ◽

Environmental Bacteria

ABSTRACTA total of 10 metallo-β-lactamase-producing isolates of six different species, includingBrevundimonas diminuta(n= 3),Rhizobium radiobacter(n= 2),Pseudomonas monteilii(n= 1),Pseudomonas aeruginosa(n= 2),Ochrobactrum anthropi(n= 1), andEnterobacter ludwigii(n= 1), were detected in the sewage water of a hospital. The presence ofblaVIM-13associated with a Tn1721-class 1 integron structure was detected in all but one of the isolates (E. ludwigii, which produced VIM-2), and in two of them (R. radiobacter), this structure was located on a plasmid, suggesting that environmental bacteria represent a reservoir for the dissemination of clinically relevant metallo-β-lactamase genes.

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First IncHI2 Plasmid Carrying mcr-9.1 , bla VIM-1 , and Double Copies of bla KPC-3 in a Multidrug-Resistant Escherichia coli Human Isolate

mSphere ◽

10.1128/msphere.00302-21 ◽

2021 ◽

Author(s):

Serena Simoni ◽

Marina Mingoia ◽

Andrea Brenciani ◽

Maria Carelli ◽

Maria M. Lleò ◽

...

Keyword(s):

Escherichia Coli ◽

Resistance Genes ◽

Multidrug Resistant ◽

Human Isolate ◽

Colistin Resistance ◽

Class 1 Integron ◽

Content Type ◽

Single Plasmid ◽

Class 1

We report a novel IncHI2 plasmid coharboring bla VIM-1 , two copies of bla KPC-3 and mcr-9.1 resistance genes in a human Escherichia coli of the new serogroup O188. The bla VIM-1 gene was included in a class 1 integron, mcr-9.1 in a cassette bracketed by IS 903 and ΔIS1R, and bla KPC-3 in two copies within a new composite Tn 4401 -like transposon. The emergence of carbapenem and colistin resistance genes in a single plasmid is of great concern for upcoming clinical therapies.

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Complete Genome Sequencing of Acinetobacter baumannii Strain K50 Discloses the Large Conjugative Plasmid pK50a Encoding Carbapenemase OXA-23 and Extended-Spectrum β-Lactamase GES-11

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.00212-18 ◽

2018 ◽

Vol 62 (5) ◽

Cited By ~ 12

Author(s):

Daniel Wibberg ◽

Ileana P. Salto ◽

Felix G. Eikmeyer ◽

Irena Maus ◽

Anika Winkler ◽

...

Keyword(s):

Acinetobacter Baumannii ◽

Genome Sequencing ◽

Antibiotic Resistance Gene ◽

Insertion Site ◽

Multidrug Resistant ◽

Conjugative Plasmid ◽

Class 1 Integron ◽

Content Type ◽

Carbapenemase Gene ◽

Class 1

ABSTRACT Multidrug-resistant (MDR) Acinetobacter baumannii strains appeared as serious emerging nosocomial pathogens in clinical environments and especially in intensive care units (ICUs). A. baumannii strain K50, recovered from a hospitalized patient in Kuwait, exhibited resistance to carbapenems and additionally to ciprofloxacin, chloramphenicol, sulfonamides, amikacin, and gentamicin. Genome sequencing revealed that the strain possesses two plasmids, pK50a (79.6 kb) and pK50b (9.5 kb), and a 3.75-Mb chromosome. A. baumannii K50 exhibits an average nucleotide identity (ANI) of 99.98% to the previously reported Iraqi clinical isolate AA-014, even though the latter strain lacked plasmid pK50a. Strain K50 belongs to sequence type 158 (ST158) (Pasteur scheme) and ST499 (Oxford scheme). Plasmid pK50a is a member of the Aci6 (replication group 6 [RG6]) group of Acinetobacter plasmids and carries a conjugative transfer module and two antibiotic resistance gene regions. The transposon Tn 2008 carries the carbapenemase gene bla OXA-23 , whereas a class 1 integron harbors the resistance genes bla GES-11 , aacA4 , dfrA7 , qacE Δ 1 , and sul1 , conferring resistance to all β-lactams and reduced susceptibility to carbapenems and resistance to aminoglycosides, trimethoprim, quaternary ammonium compounds, and sulfamethoxazole, respectively. The class 1 integron is flanked by MITEs (miniature inverted-repeat transposable elements) delimiting the element at its insertion site.

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Emergence of Multidrug-Resistant Klebsiella pneumoniae Isolates Producing VIM-4 Metallo-β-Lactamase, CTX-M-15 Extended-Spectrum β-Lactamase, and CMY-4 AmpC β-Lactamase in a Tunisian University Hospital

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.00663-06 ◽

2006 ◽

Vol 50 (12) ◽

pp. 4198-4201 ◽

Cited By ~ 69

Author(s):

Sonia Ktari ◽

Guillaume Arlet ◽

Basma Mnif ◽

Valérie Gautier ◽

Fouzia Mahjoubi ◽

...

Keyword(s):

Klebsiella Pneumoniae ◽

Gel Electrophoresis ◽

Multidrug Resistant ◽

Pulsed Field Gel Electrophoresis ◽

Clinical Isolates ◽

University Hospital ◽

Class 1 Integron ◽

Pulsed Field ◽

Extended Spectrum ◽

Class 1

ABSTRACT Klebsiella pneumoniae clinical isolates resistant to carbapenems were recovered from 11 patients in the hospital of Sfax, Tunisia. The isolates were closely related as shown by pulsed-field gel electrophoresis, and they produced VIM-4 metallo-enzyme, CTX-M-15 extended-spectrum β-lactamase, and CMY-4 AmpC enzyme. The bla VIM-4 gene is part of a class 1 integron.

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PGI2 Is a Novel SGI1-Relative Multidrug-Resistant Genomic Island Characterized in Proteus mirabilis

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.00019-18 ◽

2018 ◽

Vol 62 (5) ◽

Cited By ~ 10

Author(s):

Chang-Wei Lei ◽

Yan-Peng Chen ◽

Ling-Han Kong ◽

Jin-Xin Zeng ◽

Yong-Xiang Wang ◽

...

Keyword(s):

Escherichia Coli ◽

Multidrug Resistance ◽

Proteus Mirabilis ◽

Genomic Island ◽

Multidrug Resistant ◽

Complex Class ◽

Helper Plasmid ◽

Class 1 Integron ◽

Content Type ◽

Class 1

ABSTRACT A novel 61,578-bp genomic island named Proteus genomic island 2 (PGI2) was characterized in Proteus mirabilis of swine origin in China. The 23.85-kb backbone of PGI2 is related to those of Salmonella genomic island 1 and Acinetobacter genomic island 1. The multidrug resistance (MDR) region of PGI2 is a complex class 1 integron containing 14 different resistance genes. PGI2 was conjugally mobilized in trans to Escherichia coli in the presence of a conjugative IncC helper plasmid.

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Antimicrobial Resistance and Presence of Class 1 Integrons Among Different Serotypes of Salmonella spp. Recovered From Children with Diarrhea in Tehran, Iran

Infectious Disorders - Drug Targets ◽

10.2174/1871526519666190130171020 ◽

2020 ◽

Vol 20 (2) ◽

pp. 160-166

Author(s):

Seyedeh Hanieh Eshaghi Zadeh ◽

Hossein Fahimi ◽

Fatemeh Fardsanei ◽

Mohammad Mehdi Soltan Dallal

Keyword(s):

Antibiotic Resistance ◽

Antimicrobial Resistance ◽

Multidrug Resistant ◽

Salmonella Spp ◽

Class 1 Integron ◽

Class 1 Integrons ◽

Food Borne ◽

Class 1 ◽

Resistance Patterns ◽

C 30

Background: Salmonellosis is a major food-borne disease worldwide. The increasing prevalence of antimicrobial resistance among food-borne pathogens such as Salmonella spp. is concerning. Objective: The main objective of this study is to identify class 1 integron genes and to determine antibiotic resistance patterns among Salmonella isolates from children with diarrhea. Methods: A total of 30 Salmonella isolates were recovered from children with diarrhea. The isolates were characterized for antimicrobial susceptibility and screened for the presence of class 1 integron genes (i.e. intI1, sulI1, and qacEΔ1). Results: The most prevalent serotype was Enteritidis 36.7%, followed by Paratyphi C (30%), and Typhimurium (16.7%). The highest rates of antibiotic resistance were obtained for nalidixic acid (53.3%), followed by streptomycin (40%), and tetracycline (36.7%). Regarding class 1 integrons, 36.7%, 26.7%, and 33.3% of the isolates carried intI1, SulI, and qacEΔ1, respectively, most of which (81.8%) were multidrug-resistant (MDR). Statistical analysis revealed that the presence of class 1 integron was significantly associated with resistance to streptomycin and tetracycline (p = 0.042). However, there was no association between class 1 integron and other antibiotics used in this study (p > 0.05). Conclusion: The high frequency of integron class 1 gene in MDR Salmonella strains indicates that these mobile genetic elements are versatile among different Salmonella serotypes, and associated with reduced susceptibility to many antimicrobials.

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